FastQCFastQC Report
Tue 7 Mar 2017
1HL_11A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_11A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3568503
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA169020.4736439902110212No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG102580.28745947530378985No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG80900.2267057082479684No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT74640.2091633382401528No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA65710.1841388391714957No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61000.17094002723270796No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA60640.16993120084248214No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG58160.1629815079320376No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC54550.15286522107449538No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC53460.14981071894853387No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50030.14019884528610457No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA49850.13969443209099164No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT48340.13546296584310002No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA47090.1319600964325937No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA44780.12548679376197808No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA41840.11724804490846723No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38760.10861697468097967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGCC356.6393113E-1086.999912
AGCAGCC1100.086.999912
GCTGCTA402.0008883E-1186.99993
GCTGCCT500.086.99993
GCTGCAA850.086.99993
AGCTGCA1600.086.99992
GCAGCCC257.804101E-786.99993
GCTGCTT302.2702807E-886.999893
GCAGCCT302.2702807E-886.999893
GCAGCCG159.388781E-486.999893
CAGCAGC5100.084.441081
CTGCTGC1450.083.999911
CAGCTGC5500.083.8362661
AGCTGCT1850.082.29722
CAGCCCT186550.081.217011
AGCAGCA2250.081.199912
AGCAGCT700.080.785632
AGCTGCC1350.080.5554662
GCTGCAT650.080.30763
CAGCGTA82050.079.842681
Produced by FastQC (version 0.11.5)