##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_11A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3568503 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.814801612889214 12.0 12.0 12.0 2.0 32.0 2 14.661666530755333 12.0 12.0 12.0 12.0 22.0 3 13.703805489304619 12.0 12.0 12.0 12.0 22.0 4 13.763441140444607 12.0 12.0 12.0 12.0 22.0 5 22.457888083602565 22.0 22.0 22.0 22.0 27.0 6 26.860016090780924 27.0 22.0 32.0 22.0 32.0 7 35.04840853433499 37.0 37.0 37.0 27.0 41.0 8 37.658307138875884 41.0 37.0 41.0 32.0 41.0 9 38.58901449711546 41.0 41.0 41.0 32.0 41.0 10-11 38.9419587149009 41.0 41.0 41.0 37.0 41.0 12-13 39.11774096869191 41.0 41.0 41.0 37.0 41.0 14-15 39.07675711635944 41.0 41.0 41.0 37.0 41.0 16-17 38.986943544674055 41.0 41.0 41.0 37.0 41.0 18-19 38.928459356766695 41.0 41.0 41.0 37.0 41.0 20-21 38.944368689055324 41.0 41.0 41.0 37.0 41.0 22-23 38.92529556511512 41.0 41.0 41.0 37.0 41.0 24-25 38.94829344405764 41.0 41.0 41.0 37.0 41.0 26-27 38.915802789012645 41.0 41.0 41.0 37.0 41.0 28-29 38.86906218097617 41.0 41.0 41.0 37.0 41.0 30-31 38.747031178059814 41.0 41.0 41.0 32.0 41.0 32-33 38.787961366432924 41.0 41.0 41.0 34.5 41.0 34-35 38.76463043466686 41.0 41.0 41.0 32.0 41.0 36-37 38.68778658726082 41.0 41.0 41.0 32.0 41.0 38-39 38.60648456789864 41.0 41.0 41.0 32.0 41.0 40-41 38.64463908255087 41.0 41.0 41.0 32.0 41.0 42-43 38.38251011698743 41.0 41.0 41.0 32.0 41.0 44-45 38.486050733318706 41.0 41.0 41.0 32.0 41.0 46-47 38.50221633553342 41.0 41.0 41.0 32.0 41.0 48-49 38.44955812002961 41.0 41.0 41.0 32.0 41.0 50-51 38.336601090149 41.0 41.0 41.0 32.0 41.0 52-53 38.309903340420334 41.0 41.0 41.0 32.0 41.0 54-55 38.184623776412685 41.0 41.0 41.0 32.0 41.0 56-57 37.97812486076094 41.0 37.0 41.0 29.5 41.0 58-59 37.977718247679775 41.0 37.0 41.0 29.5 41.0 60-61 37.98075705695077 41.0 37.0 41.0 32.0 41.0 62-63 37.976483416155176 41.0 37.0 41.0 32.0 41.0 64-65 37.95590447871278 41.0 37.0 41.0 32.0 41.0 66-67 37.8336235110353 41.0 37.0 41.0 29.5 41.0 68-69 37.84516434482471 41.0 37.0 41.0 29.5 41.0 70-71 37.83676110122368 41.0 37.0 41.0 29.5 41.0 72-73 37.695518821197574 41.0 37.0 41.0 27.0 41.0 74-75 37.61403983687278 41.0 37.0 41.0 27.0 41.0 76-77 36.578397299932206 39.0 34.5 41.0 24.5 41.0 78-79 37.09483136205854 41.0 37.0 41.0 27.0 41.0 80-81 37.72932753594434 41.0 37.0 41.0 29.5 41.0 82-83 37.922482620863704 41.0 37.0 41.0 32.0 41.0 84-85 37.836429309433115 41.0 37.0 41.0 29.5 41.0 86-87 37.70098918229857 41.0 37.0 41.0 27.0 41.0 88-89 37.62623122356909 41.0 37.0 41.0 27.0 41.0 90-91 37.464111001167716 41.0 37.0 41.0 27.0 41.0 92-93 37.34923481919449 41.0 37.0 41.0 27.0 41.0 94-95 37.18980620164814 41.0 37.0 41.0 27.0 41.0 96-97 37.12930127843524 41.0 37.0 41.0 27.0 41.0 98-99 37.01746474642168 41.0 37.0 41.0 27.0 41.0 100 36.8956918349235 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5490245191244814 1101 2 -0.36837646699967586 1101 3 -0.13496150907749005 1101 4 0.17191691428254252 1101 5 -1.1381529076135166 1101 6 -0.912746435445758 1101 7 -0.2567172014950927 1101 8 -0.31980152768682757 1101 9 -0.20516733554521238 1101 10-11 -0.19664405544197905 1101 12-13 -0.14658656091922495 1101 14-15 -0.022469342717144514 1101 16-17 -0.12044457411437293 1101 18-19 0.045015429860939093 1101 20-21 0.07168121544155781 1101 22-23 0.006854404822220772 1101 24-25 -0.08475236159969057 1101 26-27 -0.03308099310089574 1101 28-29 -0.001504724878593322 1101 30-31 -0.055694720500639505 1101 32-33 -0.17846728193381978 1101 34-35 -0.03975763001713517 1101 36-37 -0.10296785551956589 1101 38-39 -0.08738330927740634 1101 40-41 -0.05856061113066602 1101 42-43 0.021119758648438847 1101 44-45 -0.011890454131169292 1101 46-47 0.059230746124001143 1101 48-49 0.03565801679894065 1101 50-51 0.01786946432590497 1101 52-53 -0.04237792252517636 1101 54-55 -0.0065799543713538355 1101 56-57 0.08692702293328125 1101 58-59 0.08439441771082556 1101 60-61 -0.015947689001102106 1101 62-63 -0.13091224406959867 1101 64-65 -0.16365908839483723 1101 66-67 -0.0289915636500524 1101 68-69 -0.12955287751700695 1101 70-71 -0.07282955125548796 1101 72-73 0.10628905415845935 1101 74-75 0.0065312551588263545 1101 76-77 -0.2520342653305647 1101 78-79 -0.08254878808113375 1101 80-81 -0.09605568013990506 1101 82-83 -0.018218634587356064 1101 84-85 -0.13206413789281868 1101 86-87 -0.1713461577510671 1101 88-89 -0.30492101129320304 1101 90-91 -0.1037648760421277 1101 92-93 -0.05375879446072673 1101 94-95 0.0714482399958527 1101 96-97 -0.09300143356218626 1101 98-99 -0.33345911744331147 1101 100 -0.3929071921385727 1102 1 1.641864422222877 1102 2 0.7101632499722594 1102 3 -0.0906239059441365 1102 4 0.07877669208107818 1102 5 -0.7669370812760725 1102 6 -0.0336866208543114 1102 7 0.252870269171531 1102 8 -0.11869205000344607 1102 9 0.023965432623946015 1102 10-11 -0.14114309666431524 1102 12-13 -0.1737063446976279 1102 14-15 -0.16925277725818688 1102 16-17 -0.11034064636880458 1102 18-19 -0.0685648980039062 1102 20-21 0.0055507959819323105 1102 22-23 0.03488136644133988 1102 24-25 -0.038956247779871944 1102 26-27 -0.009287817003567511 1102 28-29 -0.1457683852677576 1102 30-31 -0.1497961167958124 1102 32-33 -0.06965468554107446 1102 34-35 0.021049697075540053 1102 36-37 -0.13322044173102654 1102 38-39 -0.2120588218797934 1102 40-41 0.029925684732496904 1102 42-43 0.07418527736530933 1102 44-45 0.050668921351935126 1102 46-47 -0.2390255879067098 1102 48-49 -0.18007419686590964 1102 50-51 -0.08342684345212348 1102 52-53 -0.1333699198623748 1102 54-55 -0.12617997215595267 1102 56-57 -0.0945446956751681 1102 58-59 -0.10976494170088102 1102 60-61 0.05198535890840361 1102 62-63 -0.18685649219904832 1102 64-65 -0.014167734117819464 1102 66-67 -0.16941741103941155 1102 68-69 -0.27503627196110614 1102 70-71 -0.2987844946201008 1102 72-73 -0.186033045778764 1102 74-75 -0.23702697656229788 1102 76-77 0.027612940794185192 1102 78-79 -0.08273198783280122 1102 80-81 -0.2685672329926021 1102 82-83 -0.23310409114785102 1102 84-85 -0.25703094472880395 1102 86-87 -0.16118053457005743 1102 88-89 -0.2065014953851616 1102 90-91 -0.2865111917538812 1102 92-93 -0.1520695530433258 1102 94-95 -0.08716797115794606 1102 96-97 -0.19426128494654904 1102 98-99 -0.1380982266795101 1102 100 -0.32705042507728876 1103 1 1.610315334644934 1103 2 0.7573453075802874 1103 3 0.49766034501617007 1103 4 1.1211400369693294 1103 5 2.9076879044104054 1103 6 0.03473764238960442 1103 7 -0.5178580028999207 1103 8 -0.20712890914796844 1103 9 -0.25661689355783324 1103 10-11 -0.3197087775195939 1103 12-13 -0.47144772275161273 1103 14-15 -0.3661938471810515 1103 16-17 -0.41402421860785665 1103 18-19 -0.29169560389482996 1103 20-21 -0.3072640755086482 1103 22-23 -0.26014616757279896 1103 24-25 -0.29214982907548404 1103 26-27 -0.31507313497198197 1103 28-29 -0.2883881764464604 1103 30-31 -0.29246993501926966 1103 32-33 -0.4982276012318181 1103 34-35 -0.5547912614620856 1103 36-37 -0.41878533143882635 1103 38-39 -0.2172396407174304 1103 40-41 -0.3526723498906179 1103 42-43 -0.27960202888163366 1103 44-45 -0.5700287458039881 1103 46-47 -0.5440793547055733 1103 48-49 -0.4068706741776751 1103 50-51 -0.2093886081273908 1103 52-53 -0.2551925779097033 1103 54-55 -0.3686146244154074 1103 56-57 -0.3328719913040743 1103 58-59 -0.5089268979171138 1103 60-61 -0.2714542067932655 1103 62-63 -0.40333000437335187 1103 64-65 -0.5341479033817436 1103 66-67 -0.39061069044980457 1103 68-69 -0.5261462206406264 1103 70-71 -0.6667922083079958 1103 72-73 -0.44625753387511935 1103 74-75 -0.47247272570608345 1103 76-77 -0.6151769470918893 1103 78-79 -0.5561017035402003 1103 80-81 -0.40147074423001783 1103 82-83 -0.4169360035386802 1103 84-85 -0.5353999317477403 1103 86-87 -0.405579782979963 1103 88-89 -0.3394986951394898 1103 90-91 -0.6011972311238836 1103 92-93 -0.3660592785359711 1103 94-95 -0.4069277121612487 1103 96-97 -0.43516923077376646 1103 98-99 -0.4728157558010295 1103 100 -0.6176196348666991 1104 1 1.4686759239979548 1104 2 -0.5516351615347492 1104 3 -0.04844438039889987 1104 4 0.04054632298621286 1104 5 -1.1412872692493785 1104 6 -0.4613994787517228 1104 7 -0.3417819352426932 1104 8 -0.2535635102755833 1104 9 -0.1497038324732003 1104 10-11 -0.24699111873380986 1104 12-13 -0.15300536533953135 1104 14-15 -0.12609556976148895 1104 16-17 -0.156173550446006 1104 18-19 -0.19465623062753679 1104 20-21 -0.1362719563920507 1104 22-23 -0.04149822189793895 1104 24-25 -0.11952312752111283 1104 26-27 -0.1436427390588335 1104 28-29 -0.13266176458463974 1104 30-31 -0.13825771186255764 1104 32-33 -0.0670685195260603 1104 34-35 -0.16966962173994204 1104 36-37 -0.13419526197490939 1104 38-39 -0.28606155767223385 1104 40-41 -0.2082515792461308 1104 42-43 -0.1751033838951841 1104 44-45 -0.16579534082625713 1104 46-47 -0.5034825257487796 1104 48-49 -0.37637118814833315 1104 50-51 -0.18213367868154506 1104 52-53 -0.14950463597281782 1104 54-55 -0.21975392603830812 1104 56-57 -0.262528565456023 1104 58-59 -0.08197162893913656 1104 60-61 -0.11800047972726446 1104 62-63 -0.2265569613555698 1104 64-65 -0.24839563723838864 1104 66-67 -0.700369435738402 1104 68-69 -0.5270184132706035 1104 70-71 -0.33754182672371513 1104 72-73 -0.2849199641775826 1104 74-75 -0.3252084513392006 1104 76-77 -0.3344453955235025 1104 78-79 -0.4008120109881048 1104 80-81 -0.4171068218551568 1104 82-83 -0.4489682123665162 1104 84-85 -0.3265250602403995 1104 86-87 -0.39377481148196836 1104 88-89 -0.23473714637457732 1104 90-91 -0.31764943185279293 1104 92-93 -0.31108871503723634 1104 94-95 -0.37016775203291985 1104 96-97 -0.3899969727293495 1104 98-99 -0.4128620131601579 1104 100 -0.2084849745545796 1105 1 1.7253413075106288 1105 2 0.5216188969409856 1105 3 -0.11364263771538319 1105 4 -0.162156921588954 1105 5 -1.114085908304176 1105 6 0.37092888436481886 1105 7 0.11827478417362869 1105 8 0.05346406350075483 1105 9 -0.20040114647100893 1105 10-11 -0.11796712776400398 1105 12-13 -0.08671798094869843 1105 14-15 -0.1368834363375413 1105 16-17 -0.19832997092754567 1105 18-19 -0.13175176144228118 1105 20-21 -0.12164781024081606 1105 22-23 -0.15597315127435252 1105 24-25 -0.2070670605084004 1105 26-27 -0.10996586129328279 1105 28-29 -0.20695181953971797 1105 30-31 -0.22878217098308085 1105 32-33 -0.20042029819803986 1105 34-35 -0.053138187474516485 1105 36-37 -0.004403383537706418 1105 38-39 -0.09996583438439188 1105 40-41 -0.11880832773390892 1105 42-43 0.032501709424195724 1105 44-45 -0.047935090330547325 1105 46-47 -0.17682099169808652 1105 48-49 -0.25371755551933006 1105 50-51 -0.21808690170373524 1105 52-53 -0.2450484827481887 1105 54-55 -0.2330477522631682 1105 56-57 -0.029970688193714068 1105 58-59 -0.13535489500713282 1105 60-61 -0.07643436020547512 1105 62-63 -0.18288687027168038 1105 64-65 -0.4497921197925052 1105 66-67 -0.16690352653357365 1105 68-69 -0.2578931032585885 1105 70-71 -0.31227216389248014 1105 72-73 -0.24604260362534802 1105 74-75 -0.28640873162814984 1105 76-77 -0.3199133256958646 1105 78-79 -0.20226984434248862 1105 80-81 -0.4796851894827867 1105 82-83 -0.36123486379572967 1105 84-85 -0.26236806233955434 1105 86-87 -0.34695768498503554 1105 88-89 -0.14611992524503847 1105 90-91 -0.2203033050731591 1105 92-93 -0.30930773739418527 1105 94-95 -0.2152752763276311 1105 96-97 -0.13461429210036613 1105 98-99 -0.28419991171480063 1105 100 -0.5576561372725379 1106 1 1.4013402929294507 1106 2 -0.6314663210225859 1106 3 -0.03359355392331764 1106 4 -0.2864945303308044 1106 5 -1.1674405387018503 1106 6 -0.6084264452712347 1106 7 -0.05222009836117536 1106 8 0.06462719855659316 1106 9 -0.16554000013137937 1106 10-11 -0.09070300074940718 1106 12-13 0.005781659149199925 1106 14-15 -0.0060117191588915375 1106 16-17 0.10308770060865413 1106 18-19 -0.020034034378319632 1106 20-21 -0.08001090690709134 1106 22-23 0.013003972662225749 1106 24-25 -0.13103002829544863 1106 26-27 -0.11543911542530338 1106 28-29 -0.05296993118478355 1106 30-31 0.006057723994061348 1106 32-33 -0.052647920856102814 1106 34-35 -0.0013808369024488343 1106 36-37 -0.19908745804087857 1106 38-39 -0.049889398032227916 1106 40-41 -0.190935109249331 1106 42-43 0.028594543601869304 1106 44-45 -0.08057786863769678 1106 46-47 -0.4014900531419414 1106 48-49 -0.24201970876222134 1106 50-51 -0.3364011202362889 1106 52-53 -0.3486608716069952 1106 54-55 -0.2234599601426268 1106 56-57 -0.19374499872410667 1106 58-59 -0.23507725623915832 1106 60-61 -0.12286036090471697 1106 62-63 -0.25173614326942584 1106 64-65 -0.4197879380728011 1106 66-67 -0.320003140581413 1106 68-69 -0.3172251935014714 1106 70-71 -0.5451901816202351 1106 72-73 -0.439991792303168 1106 74-75 -0.3685428482912769 1106 76-77 -0.38829746806628407 1106 78-79 -0.32846591901017774 1106 80-81 -0.3411472146690784 1106 82-83 -0.2692116655342289 1106 84-85 -0.4826140982749294 1106 86-87 -0.45889546647990187 1106 88-89 -0.5443677161316316 1106 90-91 -0.48976475441608613 1106 92-93 -0.41731196024556283 1106 94-95 -0.42739472269536094 1106 96-97 -0.4448313987616359 1106 98-99 -0.7838770281105738 1106 100 -0.7399304443752897 1107 1 1.4556559747601963 1107 2 -0.6209401714770344 1107 3 -0.09474471827496878 1107 4 -0.2001216693186194 1107 5 -1.2190230145975214 1107 6 -0.2604856775651143 1107 7 -0.28669560709874986 1107 8 -0.29397001236912246 1107 9 -0.2232480554390719 1107 10-11 -0.27463107864134173 1107 12-13 -0.30265900195301043 1107 14-15 -0.28633895369540596 1107 16-17 -0.3146820862624864 1107 18-19 -0.3078469366106873 1107 20-21 -0.3278668850177553 1107 22-23 -0.2129806329427737 1107 24-25 -0.3005099196384151 1107 26-27 -0.4078157510057423 1107 28-29 -0.2901592992209814 1107 30-31 -0.46637134491945176 1107 32-33 -0.3756833488905613 1107 34-35 -0.3971458976666753 1107 36-37 -0.2531537064516911 1107 38-39 -0.30612355550594117 1107 40-41 -0.3780237958101367 1107 42-43 -0.30824332763639006 1107 44-45 -0.12167199989475819 1107 46-47 -0.4490749809276622 1107 48-49 -0.40724501811099145 1107 50-51 -0.36860853197219257 1107 52-53 -0.38254798595745854 1107 54-55 -0.3656044360856754 1107 56-57 -0.41877401867878206 1107 58-59 -0.34244881826290197 1107 60-61 -0.299527428981186 1107 62-63 -0.3626597872281465 1107 64-65 -0.3973031982258277 1107 66-67 -0.3518502705671196 1107 68-69 -0.6462977591813299 1107 70-71 -0.4790838106543944 1107 72-73 -0.36545147233348985 1107 74-75 -0.435865490962847 1107 76-77 -0.5060421832758948 1107 78-79 -0.767231939209644 1107 80-81 -0.5387466524321312 1107 82-83 -0.36748349762122245 1107 84-85 -0.2687240029631326 1107 86-87 -0.24256779368285208 1107 88-89 -0.17325885774111782 1107 90-91 -0.2590942157899363 1107 92-93 -0.32041324580982433 1107 94-95 -0.2996201583895086 1107 96-97 -0.37035267216514 1107 98-99 -0.5699877224574053 1107 100 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14-15 0.16887897479367098 2223 16-17 0.32346705614946814 2223 18-19 0.09714154428392163 2223 20-21 0.18992039419119067 2223 22-23 0.3731607815364839 2223 24-25 0.2920121053632556 2223 26-27 0.2596403662195996 2223 28-29 0.2430505472040636 2223 30-31 0.10058702103987116 2223 32-33 0.24629527141375718 2223 34-35 0.37025080419014955 2223 36-37 0.2632886606408533 2223 38-39 0.27485752601115365 2223 40-41 0.3230240394637107 2223 42-43 0.3829044218589672 2223 44-45 0.3862554759751262 2223 46-47 0.30216344586355603 2223 48-49 0.360254431018042 2223 50-51 0.34399264138296104 2223 52-53 0.2591286980354113 2223 54-55 0.3851136635668837 2223 56-57 0.3245309316838103 2223 58-59 0.4900767185411823 2223 60-61 0.4369404749154384 2223 62-63 0.2280567089964194 2223 64-65 0.2239824559137702 2223 66-67 0.4868585469096587 2223 68-69 0.37409917286087335 2223 70-71 0.48657140117516207 2223 72-73 0.5710431881529274 2223 74-75 0.38062350342219986 2223 76-77 0.45604377817100783 2223 78-79 0.4759765595489256 2223 80-81 0.332982452835374 2223 82-83 0.2554124703967915 2223 84-85 0.31162898728251776 2223 86-87 0.38167264378738963 2223 88-89 0.5216859666294127 2223 90-91 0.47919357887685265 2223 92-93 0.522628712851251 2223 94-95 0.6531357211743583 2223 96-97 0.616369419334525 2223 98-99 0.5073557416553243 2223 100 0.5942765164230721 2224 1 1.196379867165259 2224 2 -0.930789855021338 2224 3 -0.06327715498382958 2224 4 -0.31185030855912643 2224 5 -1.0828481728253685 2224 6 0.6817295123083014 2224 7 0.3821572410025169 2224 8 0.21850625345366126 2224 9 0.15901064709257184 2224 10-11 0.18763751313081656 2224 12-13 0.12025832399636016 2224 14-15 0.15460645769699966 2224 16-17 0.1895149074084017 2224 18-19 0.1107408798450038 2224 20-21 0.17428699156452154 2224 22-23 0.21696003161419242 2224 24-25 0.15661033352613885 2224 26-27 0.2032601983613489 2224 28-29 0.1634423621714305 2224 30-31 0.2971769145742229 2224 32-33 0.18657930933728295 2224 34-35 0.1945926308143342 2224 36-37 0.11029368278882146 2224 38-39 0.15774511538013058 2224 40-41 0.17399558710125262 2224 42-43 0.2126743938825939 2224 44-45 0.33381652858625444 2224 46-47 0.3142082469204368 2224 48-49 0.16309121606622767 2224 50-51 0.09972216423033586 2224 52-53 0.2941074729033062 2224 54-55 0.2666483329016671 2224 56-57 0.31492653511868696 2224 58-59 0.3292857545219121 2224 60-61 0.2934670330441307 2224 62-63 0.3594723330250176 2224 64-65 0.294045694179232 2224 66-67 0.32005241930630035 2224 68-69 0.2930179053756419 2224 70-71 0.5113137368139533 2224 72-73 0.4642696510842299 2224 74-75 0.32195689827765506 2224 76-77 0.4663055224203134 2224 78-79 0.3628451968663029 2224 80-81 0.36630872731470276 2224 82-83 0.3714049517458733 2224 84-85 0.12039783247990243 2224 86-87 0.2978475544184178 2224 88-89 0.45345989225888417 2224 90-91 0.35208655952651924 2224 92-93 0.24052227634208379 2224 94-95 0.5045115662940347 2224 96-97 0.3305294480880221 2224 98-99 0.40750436615018515 2224 100 0.45944845456419614 2225 1 1.2944382847391314 2225 2 1.54637285882003 2225 3 0.9555141552805573 2225 4 0.8913305817985719 2225 5 3.167382678652082 2225 6 -0.2172129449234248 2225 7 0.20998480709894096 2225 8 0.4414174120850731 2225 9 0.18411999779241484 2225 10-11 0.1454449100981563 2225 12-13 0.1929549122545211 2225 14-15 0.1383997119738254 2225 16-17 0.0956691647956518 2225 18-19 0.07056640472836762 2225 20-21 0.03262364591910938 2225 22-23 0.21767299817717856 2225 24-25 0.2178272853146339 2225 26-27 0.20267699462884536 2225 28-29 0.18527921092725563 2225 30-31 0.12662627305012109 2225 32-33 0.026266323334169783 2225 34-35 0.08075830927467109 2225 36-37 0.03325174072038806 2225 38-39 0.1320248587704853 2225 40-41 0.14353850117590383 2225 42-43 0.3406215167441786 2225 44-45 0.27965755696883576 2225 46-47 0.32569444443210216 2225 48-49 0.16978979693714535 2225 50-51 0.11285494028010135 2225 52-53 0.19526197329210504 2225 54-55 0.2976069740369738 2225 56-57 0.39735193364123234 2225 58-59 0.44433411183140237 2225 60-61 0.33549998405501924 2225 62-63 0.27686395433139666 2225 64-65 0.3017046171963358 2225 66-67 0.3785142194618203 2225 68-69 0.3840448299479533 2225 70-71 0.241211433159215 2225 72-73 0.2533348241013371 2225 74-75 0.22091320659802705 2225 76-77 0.5859344590455038 2225 78-79 0.47245372136086417 2225 80-81 0.22120472264906965 2225 82-83 0.18741049218132844 2225 84-85 0.23225144834304956 2225 86-87 0.3243497900327199 2225 88-89 0.28539199762435885 2225 90-91 0.45101710968204856 2225 92-93 0.2489852915053774 2225 94-95 0.445420149108962 2225 96-97 0.5480979745265842 2225 98-99 0.4095504392450593 2225 100 0.497544294377569 2226 1 1.436301622157579 2226 2 1.4900866810769724 2226 3 0.8598723265829911 2226 4 1.141349096202779 2226 5 3.20127001073838 2226 6 -0.20327816818324962 2226 7 -0.004619832730206497 2226 8 0.1601604393215581 2226 9 0.2550671947116214 2226 10-11 0.1110229297974783 2226 12-13 0.11876470840496012 2226 14-15 0.06037690700575382 2226 16-17 0.14651903567261115 2226 18-19 0.04517300350092057 2226 20-21 0.09866751191782441 2226 22-23 0.19926561917179697 2226 24-25 0.15363414304994905 2226 26-27 0.10821411541203929 2226 28-29 0.19741677653394873 2226 30-31 0.17075362348359135 2226 32-33 0.12232239689488722 2226 34-35 0.14218599678975608 2226 36-37 0.17290624999311888 2226 38-39 0.21001374057214406 2226 40-41 0.19011285860663207 2226 42-43 0.29576195148628415 2226 44-45 0.0696760235939351 2226 46-47 0.25304177226605873 2226 48-49 0.1699417344994103 2226 50-51 0.15153380017503792 2226 52-53 0.2937827321198938 2226 54-55 0.2669404315959838 2226 56-57 0.29710515455033004 2226 58-59 0.25265341005186315 2226 60-61 0.07687669126225671 2226 62-63 0.31226851198047 2226 64-65 0.1988954445632629 2226 66-67 0.29276123267035814 2226 68-69 0.35504219493017075 2226 70-71 0.31385669956357276 2226 72-73 0.3234903212071174 2226 74-75 0.307321961641712 2226 76-77 0.37344093908697573 2226 78-79 0.28817089771115434 2226 80-81 0.16368679951131782 2226 82-83 0.2096062766306659 2226 84-85 0.12169871573643576 2226 86-87 0.19613240464882864 2226 88-89 0.21201379970896284 2226 90-91 0.2578908510011786 2226 92-93 0.13952837273225072 2226 94-95 0.4825691219775976 2226 96-97 0.5249289296548483 2226 98-99 0.32792007275541124 2226 100 0.4197412733045951 2227 1 1.2265865860121288 2227 2 1.533583595071656 2227 3 0.7527130418543599 2227 4 -0.0430160323656974 2227 5 3.190695883281265 2227 6 0.08531289730520086 2227 7 0.18087818024616098 2227 8 0.30526268556773317 2227 9 0.16349278441433057 2227 10-11 0.08480883597644606 2227 12-13 0.05964058918544879 2227 14-15 0.1470899899350684 2227 16-17 0.08591776698683162 2227 18-19 -0.030810770805949517 2227 20-21 0.15309172265565962 2227 22-23 0.09348610879955999 2227 24-25 0.19274641536123482 2227 26-27 0.16459001857214162 2227 28-29 -6.547201286366544E-4 2227 30-31 0.11756690992450558 2227 32-33 0.03566873897150913 2227 34-35 0.12822745334997876 2227 36-37 0.10513898269582 2227 38-39 0.07675821209432598 2227 40-41 0.12857488421843044 2227 42-43 0.21922740070218083 2227 44-45 0.2698436325850153 2227 46-47 0.2674657639694118 2227 48-49 0.11832854557645334 2227 50-51 0.015950078055503525 2227 52-53 0.10731277746003087 2227 54-55 0.1838591783759398 2227 56-57 0.3089426347777078 2227 58-59 0.2021911520730626 2227 60-61 0.12466220508381554 2227 62-63 0.19942409216947254 2227 64-65 0.14441166597092092 2227 66-67 0.10619256964727697 2227 68-69 0.18093773643577293 2227 70-71 0.21874602525164732 2227 72-73 0.2216773347482146 2227 74-75 0.14282397437808925 2227 76-77 0.37980589052322244 2227 78-79 0.3354671551738022 2227 80-81 0.18307368391730705 2227 82-83 0.18483547716434146 2227 84-85 0.3222131610601906 2227 86-87 0.24297716539175696 2227 88-89 0.1807321527982495 2227 90-91 0.25643879526737834 2227 92-93 0.2602871748231692 2227 94-95 0.41379134388237304 2227 96-97 0.28956133742465795 2227 98-99 0.3431425403597359 2227 100 0.4124641861203031 2228 1 1.4363832062601674 2228 2 1.4595640890390005 2228 3 -0.10743079263310662 2228 4 -0.15279055840199263 2228 5 3.0917619387396833 2228 6 0.16152838094437882 2228 7 0.28814129191388815 2228 8 0.299904352133737 2228 9 0.23868636205171612 2228 10-11 0.21814633226971836 2228 12-13 0.20433643917549205 2228 14-15 0.23547560446003502 2228 16-17 0.1627257417892949 2228 18-19 0.17620069285569429 2228 20-21 0.2094338135318381 2228 22-23 0.1400113751088483 2228 24-25 0.2208859463482682 2228 26-27 0.18888244851848413 2228 28-29 0.19941138715571327 2228 30-31 0.14726628446737067 2228 32-33 0.30502289827505535 2228 34-35 0.2878901834541807 2228 36-37 0.27909563517725644 2228 38-39 0.23663535736630337 2228 40-41 0.07418522805034655 2228 42-43 0.4183563549133922 2228 44-45 0.3502041189382368 2228 46-47 0.24273943799523323 2228 48-49 0.27759683360236664 2228 50-51 0.23159952123850758 2228 52-53 0.26288369461920524 2228 54-55 0.1561397101801063 2228 56-57 0.3610933523719808 2228 58-59 0.46773435210204894 2228 60-61 0.3021838385123914 2228 62-63 0.24825395621797952 2228 64-65 0.3451619339655281 2228 66-67 0.38653713803665823 2228 68-69 0.3515716844454033 2228 70-71 0.4803441225776268 2228 72-73 0.3755476405562632 2228 74-75 0.5228451957320885 2228 76-77 0.6255046306855476 2228 78-79 0.6122261875954109 2228 80-81 0.47121483982255796 2228 82-83 0.39841646180244084 2228 84-85 0.267088200173184 2228 86-87 0.5814284390821456 2228 88-89 0.5584586155461224 2228 90-91 0.43581564107901016 2228 92-93 0.5819898085545248 2228 94-95 0.8002609966837255 2228 96-97 0.618530901576392 2228 98-99 0.7136659783437764 2228 100 0.6629765029614347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 30.0 13 39.0 14 162.0 15 598.0 16 2278.0 17 5640.0 18 9201.0 19 11847.0 20 14088.0 21 16252.0 22 18254.0 23 21189.0 24 24093.0 25 27326.0 26 30833.0 27 34961.0 28 39742.0 29 45243.0 30 51306.0 31 59631.0 32 70222.0 33 83576.0 34 102395.0 35 132438.0 36 186281.0 37 308214.0 38 761108.0 39 1509613.0 40 1943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.4252500997112 18.34550199770048 20.67340299316907 35.55584490941925 2 18.028818398576792 50.47148819205689 14.715761897342022 16.783931512024296 3 42.36361858179746 15.446449113255614 23.182382080104738 19.00755022484218 4 17.286828678580346 16.69394701363569 21.265471823899265 44.7537524838847 5 17.563499316099776 34.70603779792255 26.521064995601794 21.209397890375882 6 17.693890127036465 28.997173324500498 29.376379955404268 23.93255659305877 7 20.224867402381335 25.838706034435166 35.906429110470135 18.029997452713364 8 21.708794976492943 28.167777916958453 32.92125577588137 17.20217133066723 9 21.123367417653846 28.383330489003374 27.12050403208292 23.37279806125986 10-11 19.859588180253738 30.198741601170013 28.43346355600654 21.50820666256971 12-13 24.195580051354867 26.638789430750094 27.36480815624927 21.800822361645768 14-15 21.835052401525235 27.46433168194058 28.575806157371876 22.124809759162314 16-17 20.792794625645545 29.532860137710408 28.338227542473692 21.336117694170355 18-19 21.32803307157091 29.457632514250378 29.66141544507599 19.55291896910273 20-21 22.840249819041766 28.6583337606834 29.873521193620967 18.627895226653866 22-23 21.661548273884033 30.231262240777156 28.740006663858768 19.367182821480043 24-25 22.22048012850206 28.440273134140565 26.620028062187423 22.71921867516995 26-27 20.301089840753953 26.537990860593364 30.0288664462381 23.132052852414585 28-29 19.612631781893242 28.027463148989305 30.615013460871836 21.744891608245613 30-31 20.25594905121223 29.82049150648014 28.23449356694203 21.6890658753656 32-33 20.857331491664713 29.86844903871455 28.608452900277793 20.665766569342946 34-35 21.420845099471684 29.08984523762485 29.842205541091037 19.647104121812422 36-37 21.733441165665266 28.13646226442853 28.7822933033824 21.347803266523805 38-39 21.54167795183125 29.2146183993697 28.852250772073397 20.391452876725648 40-41 21.019959332551668 29.700842605317256 29.731710297114493 19.547487765016577 42-43 20.49436198445929 29.70025067966261 29.32549922607506 20.479888109803042 44-45 20.459461572541763 30.13403659741914 28.78243341815882 20.624068411880277 46-47 19.908312657816186 28.37676721551191 28.817535099104468 22.897385027567434 48-49 20.96247361989047 28.005272799266244 30.15118664605298 20.8810669347903 50-51 21.78561990840417 30.532565055991267 28.22961056779271 19.452204467811853 52-53 20.4693599233618 30.24187915472114 28.237160306468724 21.051600615448336 54-55 22.240705279845017 28.457940284183785 28.111119021299206 21.19023541467199 56-57 21.490293489279498 28.49242825741318 29.34269208358919 20.674586169718133 58-59 21.139908179419674 27.91842007277876 29.117544552712754 21.824127195088813 60-61 19.740052952280177 30.211971654164664 29.497454950208716 20.55052044334644 62-63 20.547118433539026 30.860649971665545 27.539324916888404 21.05290667790703 64-65 19.767583115770023 30.32960753974743 28.649994962871673 21.25281438161088 66-67 20.183239302306877 30.31733474793212 29.305243683415704 20.1941822663453 68-69 20.884807999320724 29.91762932523806 29.19268948351732 20.004873191923895 70-71 19.730766654812957 30.924606200415134 28.96672638358438 20.377900761187533 72-73 20.13837455089711 30.69013813355348 28.487603905615323 20.683883409934083 74-75 20.194560576241635 29.205313824872782 30.277135818577143 20.322989780308436 76-77 19.560583247372918 30.532537033035982 28.551202002632476 21.355677716958624 78-79 19.62853891393674 29.430324144326065 28.49151310787745 22.449623833859743 80-81 20.99078182819368 29.465559010170956 28.372198924196333 21.17146023743903 82-83 20.349359381230727 28.267329465605044 29.590405276386207 21.792905876778022 84-85 20.936524363297437 28.87731914475061 28.498462800787895 21.687693691164053 86-87 21.220018590428534 28.09306871817118 29.704654865079277 20.98225782632101 88-89 20.490118685622516 27.29325153993145 30.555824108877026 21.660805665569008 90-91 20.832447107372477 27.63522967474036 29.764441840177803 21.767881377709365 92-93 20.733105730890518 28.102680591833607 30.645021175546162 20.519192501729716 94-95 21.1164177247434 28.373844718639724 29.562340286669226 20.94739726994765 96-97 20.309426669950955 28.77353612985613 29.945946521552596 20.971090678640316 98-99 22.1909859680656 28.578440315168574 29.03420565990837 20.196368056857455 100 20.70506876412882 30.156314846869964 28.894469193384452 20.244147195616762 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 6.0 2 17.5 3 25.0 4 31.0 5 36.5 6 44.5 7 53.0 8 67.0 9 76.5 10 90.5 11 109.5 12 128.5 13 153.5 14 175.0 15 228.0 16 297.0 17 386.0 18 587.0 19 895.0 20 1425.5 21 2347.5 22 3625.0 23 5527.0 24 8536.5 25 12895.0 26 18449.5 27 24947.5 28 32353.0 29 41087.5 30 51507.0 31 63058.5 32 75655.5 33 89614.5 34 104615.0 35 121826.5 36 139241.5 37 152274.5 38 161723.5 39 169064.5 40 174113.0 41 175325.0 42 172516.0 43 168597.0 44 165544.5 45 160866.0 46 150014.0 47 136324.0 48 127766.5 49 124023.5 50 119751.5 51 111001.0 52 96418.5 53 80429.0 54 67370.5 55 55970.5 56 44335.0 57 33325.0 58 24065.5 59 17583.0 60 14027.0 61 11994.5 62 9818.5 63 7498.5 64 5724.5 65 4679.5 66 4418.0 67 4253.0 68 3553.5 69 2971.0 70 2985.0 71 2788.0 72 1920.0 73 982.5 74 495.5 75 322.0 76 236.5 77 168.0 78 133.0 79 113.0 80 83.5 81 66.5 82 52.5 83 37.5 84 31.5 85 28.5 86 24.0 87 20.5 88 21.5 89 21.5 90 22.0 91 20.5 92 19.0 93 20.5 94 20.0 95 21.5 96 22.5 97 27.5 98 57.0 99 122.0 100 121.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.331502313435074 2 0.00636121084947946 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.362754634086058E-4 30-31 0.01039651641038273 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.706312703113883E-4 40-41 0.0013170788983503728 42-43 1.2610329877822717E-4 44-45 0.0 46-47 0.0020036413028096094 48-49 0.0 50-51 0.0 52-53 0.0013310903759923979 54-55 0.018803402995597872 56-57 0.01216196259327791 58-59 0.015875004168414596 60-61 1.961606869883534E-4 62-63 0.011461388711176647 64-65 4.2034432926075724E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2418364227240389E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3568503.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.13019385718326 #Duplication Level Percentage of deduplicated Percentage of total 1 84.93889935361823 61.2665927639226 2 9.172111636079281 13.231723807802496 3 2.6509063099019445 5.736311580913727 4 1.139562168015832 3.287873603651761 5 0.599591402504055 2.162432204885894 6 0.3660490320577089 1.58419125861341 7 0.2365395064164161 1.1943148316888978 8 0.16992524297327352 0.9805392573512949 9 0.13146150646493168 0.8534109551465585 >10 0.5576233040744376 6.765748586787219 >50 0.026031779455124332 1.2754188111143858 >100 0.01070485459673346 1.3520634214221279 >500 4.75123077700928E-4 0.2125070197869742 >1k 1.1878076438851735E-4 0.0968718969126298 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4011477642025241E-5 1.4011477642025241E-5 16-17 0.0 0.0 0.0 2.8022955284050482E-5 2.8022955284050482E-5 18-19 0.0 0.0 0.0 2.8022955284050482E-5 2.8022955284050482E-5 20-21 0.0 0.0 0.0 2.8022955284050482E-5 2.8022955284050482E-5 22-23 0.0 1.4011477642025241E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 24-25 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 26-27 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 28-29 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 30-31 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 32-33 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 34-35 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 36-37 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 38-39 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 40-41 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 42-43 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 44-45 0.0 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 46-47 2.8022955284050482E-5 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 48-49 2.8022955284050482E-5 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 50-51 2.8022955284050482E-5 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 52-53 7.00573882101262E-5 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 54-55 1.1209182113620193E-4 2.8022955284050482E-5 0.0 2.8022955284050482E-5 2.8022955284050482E-5 56-57 1.1209182113620193E-4 2.8022955284050482E-5 0.0 5.6045910568100965E-5 2.8022955284050482E-5 58-59 2.2418364227240386E-4 2.8022955284050482E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 60-61 2.5220659755645434E-4 2.8022955284050482E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 62-63 2.802295528405048E-4 2.8022955284050482E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 64-65 3.2226398576658055E-4 2.8022955284050482E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 66-67 3.5028694105063103E-4 4.2034432926075724E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 68-69 4.06332851618732E-4 5.6045910568100965E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 70-71 4.483672845448077E-4 7.00573882101262E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 72-73 5.464476280389844E-4 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 74-75 6.725509268172116E-4 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 76-77 7.986542255954388E-4 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 78-79 8.967345690896154E-4 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 80-81 0.0011489411666460699 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 82-83 0.001499228107696701 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 84-85 0.001611319928832903 8.406886585215145E-5 0.0 8.406886585215145E-5 2.8022955284050482E-5 86-87 0.001625331406474928 1.401147764202524E-4 0.0 8.406886585215145E-5 2.8022955284050482E-5 88 0.001625331406474928 1.401147764202524E-4 0.0 8.406886585215145E-5 2.8022955284050482E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 13205 0.0 79.87201 8 CACCGGC 12735 0.0 78.5074 9 CTTCACC 14670 0.0 74.45541 6 CGTAGTG 2715 0.0 70.71042 4 CCTTCAC 16425 0.0 69.92919 5 GTGTGGC 5070 0.0 67.58415 7 TTCACCG 16160 0.0 66.71902 7 CGGGGCA 1405 0.0 65.81424 94 CGGGCCC 1155 0.0 64.21035 7 AGGCAGC 45 2.2230051E-7 62.590916 2 GGCCCGA 1185 0.0 61.79256 9 GGGCCCG 1195 0.0 61.668262 8 GCGTAGT 1835 0.0 60.623672 3 ACGGGCC 1225 0.0 60.158016 6 ACCTTCA 6920 0.0 59.012375 4 CAGCGTA 1365 0.0 58.181625 1 AACGGGC 1270 0.0 58.026432 5 TGTGGCA 6120 0.0 55.298557 8 GCCTTCA 5115 0.0 54.69285 4 TGTGTGG 5975 0.0 54.519455 6 >>END_MODULE