FastQCFastQC Report
Tue 7 Mar 2017
1HL_11A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_11A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3568503
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG132050.079.872018
CACCGGC127350.078.50749
CTTCACC146700.074.455416
CGTAGTG27150.070.710424
CCTTCAC164250.069.929195
GTGTGGC50700.067.584157
TTCACCG161600.066.719027
CGGGGCA14050.065.8142494
CGGGCCC11550.064.210357
AGGCAGC452.2230051E-762.5909162
GGCCCGA11850.061.792569
GGGCCCG11950.061.6682628
GCGTAGT18350.060.6236723
ACGGGCC12250.060.1580166
ACCTTCA69200.059.0123754
CAGCGTA13650.058.1816251
AACGGGC12700.058.0264325
TGTGGCA61200.055.2985578
GCCTTCA51150.054.692854
TGTGTGG59750.054.5194556
Produced by FastQC (version 0.11.5)