FastQCFastQC Report
Mon 6 Mar 2017
1HL_12A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_12A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2524813
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA115290.4566278769952468No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG82390.3263211968569553No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA78690.3116666462031049No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG69560.2755055522923876No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC63440.2512661333730458No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG55310.21906572882823402No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG51360.20342100583290723No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT51190.2027476886407033No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA48620.19256871697032613No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA45190.1789835524452702No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG43960.17411190452520642No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA43410.1719335253739584No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA43110.17074531856418673No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG42230.16725991192218986No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG38700.1532786784605434No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT35880.14210953444868987No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC34720.13751513478423946No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA34080.13498029359005995No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA33980.1345842246534694No Hit
CAGCGGTTCTGACGTGCAAATCGATCGTCAAACTTGAGCATAGGGGCGAA33650.13327719716272057No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC33130.1312176386924497No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA33070.13097999733049537No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT33020.1307819628622001No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA31720.1256330666865229No Hit
CAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTTGGCACTCTCATGCGA28680.11359257101417015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAC302.2700988E-887.03
GCAGCTT202.699153E-587.03
AGCAGCC402.0008883E-1187.02
CAGCAGC2450.085.224491
CAGCTGC4100.082.7560961
AGCAGCG1000.082.652
AGCTGCA850.081.8823552
AGCTGCC1650.081.727272
AGCAGCA800.081.56252
CAGCCCT136200.081.283041
CAGCGTA51600.080.845931
AGCCCTT114200.080.0293352
GCTGCAA550.079.0909043
AGCTGCT1100.079.0909042
GCCCTTC105600.078.308243
CAGCTAG39200.078.0114751
GCTGCCC1400.077.6785663
CAGCGAT48400.076.844011
CGTAGTG29600.076.4189154
CTGCTGC1200.076.1251
Produced by FastQC (version 0.11.5)