##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1HL_12A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2524813
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.80113537121363	12.0	12.0	12.0	2.0	32.0
2	14.403352248265515	12.0	12.0	12.0	12.0	22.0
3	13.682106754044755	12.0	12.0	12.0	12.0	22.0
4	13.860278365170014	12.0	12.0	12.0	12.0	22.0
5	22.43072892923159	22.0	22.0	22.0	22.0	27.0
6	26.93187455863068	27.0	22.0	32.0	22.0	32.0
7	34.96146486888336	37.0	37.0	37.0	27.0	41.0
8	37.572746971755926	41.0	37.0	41.0	27.0	41.0
9	38.511894148200284	41.0	37.0	41.0	32.0	41.0
10-11	38.86916575603817	41.0	41.0	41.0	37.0	41.0
12-13	39.05592315153637	41.0	41.0	41.0	37.0	41.0
14-15	39.009757752356315	41.0	41.0	41.0	37.0	41.0
16-17	38.906081163238625	41.0	41.0	41.0	37.0	41.0
18-19	38.834673498591776	41.0	41.0	41.0	37.0	41.0
20-21	38.84979184597037	41.0	41.0	41.0	37.0	41.0
22-23	38.83344489275048	41.0	41.0	41.0	34.5	41.0
24-25	38.85038594937526	41.0	41.0	41.0	37.0	41.0
26-27	38.80986710698971	41.0	41.0	41.0	37.0	41.0
28-29	38.750166051901665	41.0	41.0	41.0	32.0	41.0
30-31	38.63720402263455	41.0	41.0	41.0	32.0	41.0
32-33	38.70327743084339	41.0	41.0	41.0	32.0	41.0
34-35	38.66834137815355	41.0	41.0	41.0	32.0	41.0
36-37	38.596995104191876	41.0	41.0	41.0	32.0	41.0
38-39	38.497219398030666	41.0	41.0	41.0	32.0	41.0
40-41	38.54156248403347	41.0	41.0	41.0	32.0	41.0
42-43	38.281487381441714	41.0	41.0	41.0	32.0	41.0
44-45	38.396892759978655	41.0	41.0	41.0	32.0	41.0
46-47	38.40045104330499	41.0	41.0	41.0	32.0	41.0
48-49	38.3459640377327	41.0	41.0	41.0	32.0	41.0
50-51	38.236954380383814	41.0	41.0	41.0	32.0	41.0
52-53	38.201836334017614	41.0	41.0	41.0	32.0	41.0
54-55	38.0705709690183	41.0	37.0	41.0	32.0	41.0
56-57	37.86544983727508	41.0	37.0	41.0	29.5	41.0
58-59	37.84655774506865	41.0	37.0	41.0	29.5	41.0
60-61	37.873784117873285	41.0	37.0	41.0	29.5	41.0
62-63	37.8696315331076	41.0	37.0	41.0	29.5	41.0
64-65	37.83501847463555	41.0	37.0	41.0	27.0	41.0
66-67	37.712417117624156	41.0	37.0	41.0	27.0	41.0
68-69	37.707795389203085	41.0	37.0	41.0	27.0	41.0
70-71	37.67568073358304	41.0	37.0	41.0	27.0	41.0
72-73	37.565020855009855	41.0	37.0	41.0	27.0	41.0
74-75	37.44974875367007	41.0	37.0	41.0	27.0	41.0
76-77	36.37911164113936	39.0	34.5	41.0	24.5	41.0
78-79	36.91258263483276	41.0	37.0	41.0	27.0	41.0
80-81	37.5828235596062	41.0	37.0	41.0	27.0	41.0
82-83	37.76631140603284	41.0	37.0	41.0	27.0	41.0
84-85	37.69120980444889	41.0	37.0	41.0	27.0	41.0
86-87	37.55943846138308	41.0	37.0	41.0	27.0	41.0
88-89	37.477302279416335	41.0	37.0	41.0	27.0	41.0
90-91	37.32389151196544	41.0	37.0	41.0	27.0	41.0
92-93	37.212774569839425	41.0	37.0	41.0	27.0	41.0
94-95	37.054752965863216	41.0	37.0	41.0	27.0	41.0
96-97	36.978021738639654	41.0	37.0	41.0	27.0	41.0
98-99	36.86159668062545	41.0	37.0	41.0	27.0	41.0
100	36.72464614211032	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5441005393009348
1101	2	-0.5136323213407401
1101	3	-0.027590825890751347
1101	4	0.11134666256205072
1101	5	-1.0873024599524825
1101	6	-0.7823937451293652
1101	7	-0.11970682423795864
1101	8	0.09853304739071689
1101	9	0.07087486828272205
1101	10-11	-0.0570480793226551
1101	12-13	-0.09815189468618968
1101	14-15	-0.06708117261589308
1101	16-17	-0.012523350824935164
1101	18-19	0.10792652772428823
1101	20-21	0.14172716380574002
1101	22-23	0.01837232128939803
1101	24-25	0.02061288783597348
1101	26-27	0.038641321267263606
1101	28-29	0.008253594860185842
1101	30-31	-0.01231719017085453
1101	32-33	-0.07545840997668307
1101	34-35	0.05894710690604654
1101	36-37	0.06503322232959619
1101	38-39	0.06587455123709418
1101	40-41	-0.008493961119370397
1101	42-43	0.12076010925407843
1101	44-45	0.06194868083017013
1101	46-47	0.06832978229905962
1101	48-49	0.20927079533237958
1101	50-51	0.14605820385557422
1101	52-53	0.17748649677798767
1101	54-55	0.14460926695392828
1101	56-57	0.17239299264905128
1101	58-59	0.008946485378913849
1101	60-61	0.07439497737622247
1101	62-63	0.0993350790434846
1101	64-65	0.09877426547912194
1101	66-67	0.009312450859717103
1101	68-69	-0.020167808259380138
1101	70-71	0.12139001403509297
1101	72-73	0.3188812899210447
1101	74-75	0.18156442324027466
1101	76-77	-0.24139035943961318
1101	78-79	-0.02628821139916937
1101	80-81	0.06103296456210927
1101	82-83	-0.048957465393712596
1101	84-85	0.1667957152877051
1101	86-87	0.10211944743809909
1101	88-89	-0.18080500582467351
1101	90-91	0.01343685094984437
1101	92-93	0.1261838159468951
1101	94-95	0.15946736828374242
1101	96-97	0.09372143227963647
1101	98-99	-0.07693680268849334
1101	100	-0.2778199975704112
1102	1	1.3836499614845366
1102	2	0.7898061765769953
1102	3	-0.13257967476445742
1102	4	0.04066967072607852
1102	5	-0.8028361273449143
1102	6	0.05458731239857073
1102	7	-0.028621483631191325
1102	8	-0.18904663200707716
1102	9	-0.36439618155122133
1102	10-11	-0.35224872182790534
1102	12-13	-0.3181939863752987
1102	14-15	-0.3888094972038587
1102	16-17	-0.2702258693574535
1102	18-19	-0.2859841554001079
1102	20-21	-0.29304404974504905
1102	22-23	-0.17938367740248395
1102	24-25	-0.2411786126646689
1102	26-27	-0.32441323289877033
1102	28-29	-0.3259525582668701
1102	30-31	-0.2891406432285635
1102	32-33	-0.4031274034221539
1102	34-35	-0.1417107499567578
1102	36-37	-0.28463240547749535
1102	38-39	-0.2524810651593157
1102	40-41	-0.29091186384309253
1102	42-43	-0.33012393135415863
1102	44-45	-0.20798957540071683
1102	46-47	-0.6116391549312326
1102	48-49	-0.5094677225222526
1102	50-51	-0.227267182999924
1102	52-53	-0.3013390889414609
1102	54-55	-0.30955304924842153
1102	56-57	-0.26523877112361305
1102	58-59	-0.23154536402745407
1102	60-61	-0.23564673254259105
1102	62-63	-0.6377925459554845
1102	64-65	-0.4325964453872544
1102	66-67	-0.4936731341810301
1102	68-69	-0.6365516922206069
1102	70-71	-0.4761024015628763
1102	72-73	-0.36078000913618524
1102	74-75	-0.4081447263795397
1102	76-77	-0.3016461334122198
1102	78-79	-0.4165206691049761
1102	80-81	-0.372552943145223
1102	82-83	-0.42235486341711237
1102	84-85	-0.3746000005224914
1102	86-87	-0.37305386920237993
1102	88-89	-0.4278712829644391
1102	90-91	-0.6121250735036767
1102	92-93	-0.4955851500070736
1102	94-95	-0.3486877702332194
1102	96-97	-0.27073456223095604
1102	98-99	-0.4713572198442435
1102	100	-0.7997056357560055
1103	1	1.4763704746334003
1103	2	0.6227764754760319
1103	3	0.3158560962558319
1103	4	1.0461067732146585
1103	5	2.987503996312533
1103	6	0.09731784029045087
1103	7	-0.2846366912647227
1103	8	-0.3194798353129258
1103	9	0.09684840474137957
1103	10-11	-0.005677868341962267
1103	12-13	-0.13617367204370368
1103	14-15	-0.22733120621154512
1103	16-17	-0.1864563406085793
1103	18-19	-0.05194125289774121
1103	20-21	-0.047811416294486264
1103	22-23	0.009772243937455016
1103	24-25	-0.09095048995287414
1103	26-27	0.05050724429552389
1103	28-29	0.036434509088024924
1103	30-31	0.001340569277715531
1103	32-33	-0.2765631128763957
1103	34-35	-0.1649562408597589
1103	36-37	-0.1678287954089015
1103	38-39	2.2004237276007643E-4
1103	40-41	-0.27577554431039886
1103	42-43	0.049930640786470804
1103	44-45	-0.3096920611912779
1103	46-47	-0.255402330064463
1103	48-49	-0.1376099507533155
1103	50-51	-0.11763724827635258
1103	52-53	0.04986050488682281
1103	54-55	-0.03497547439231852
1103	56-57	0.04911067281188508
1103	58-59	-0.06056404644846225
1103	60-61	-0.00838276443872843
1103	62-63	0.10199077440793758
1103	64-65	-0.05500061578892712
1103	66-67	-0.14942311530077745
1103	68-69	-0.18768013622505464
1103	70-71	-0.3310960027987875
1103	72-73	0.03220845995438282
1103	74-75	-0.17813224489039925
1103	76-77	-0.165512222343132
1103	78-79	-0.14616006037277884
1103	80-81	-0.04339809715883547
1103	82-83	-0.048636360604781714
1103	84-85	-0.1436848582310546
1103	86-87	0.07225403899690264
1103	88-89	0.08157048540932266
1103	90-91	-0.251600276343261
1103	92-93	-0.1733410923615537
1103	94-95	-0.1837706052920467
1103	96-97	0.03338855672120644
1103	98-99	-0.11068230414867486
1103	100	0.06942999341612222
1104	1	1.5047454514469099
1104	2	-0.5181687116592908
1104	3	0.0899128942095615
1104	4	0.005889601218083129
1104	5	-1.1294222234596027
1104	6	-0.5467430824179935
1104	7	-0.0840094763446686
1104	8	-0.08998299843843682
1104	9	0.01132523614874259
1104	10-11	0.06730506965492111
1104	12-13	0.06329004882673672
1104	14-15	0.025566291599972146
1104	16-17	0.16000932629999198
1104	18-19	0.1389803816441102
1104	20-21	0.0920105480650264
1104	22-23	0.13894581231400593
1104	24-25	0.025450169357036145
1104	26-27	-0.004256094852060244
1104	28-29	-0.0036674423196032535
1104	30-31	0.014884835950027764
1104	32-33	0.04831898178306204
1104	34-35	-0.018293346404668398
1104	36-37	0.12799655824610312
1104	38-39	-0.023595738108930675
1104	40-41	0.050917537328828644
1104	42-43	0.1699178841486244
1104	44-45	0.07634501360102064
1104	46-47	-0.15356777015220047
1104	48-49	-0.1716819907740188
1104	50-51	-0.13833353046489094
1104	52-53	-0.029282514270903448
1104	54-55	0.06432006306240368
1104	56-57	0.03816278465375689
1104	58-59	-0.0492552422530963
1104	60-61	0.037788149287997896
1104	62-63	-0.06939481775063427
1104	64-65	-0.18724964846479253
1104	66-67	-0.545057668541844
1104	68-69	-0.40675474709878756
1104	70-71	-0.18159591574775646
1104	72-73	-0.19781586958993103
1104	74-75	-0.30007010265389056
1104	76-77	-0.26288195900967537
1104	78-79	0.060402387762700016
1104	80-81	-0.07296351723297079
1104	82-83	-0.05330447242712921
1104	84-85	0.10553432651565231
1104	86-87	-0.02505793483246066
1104	88-89	-0.1543166704136567
1104	90-91	-0.19677419129684637
1104	92-93	-0.1104324813055868
1104	94-95	-0.12674953243277542
1104	96-97	-0.15293808480099358
1104	98-99	-0.2204114574593845
1104	100	-0.23341201952926127
1105	1	1.7356359213281678
1105	2	0.1092252777788989
1105	3	0.05851827370005935
1105	4	-0.28641160346879957
1105	5	-1.1160865249170335
1105	6	0.40737297034082687
1105	7	0.04570668284856794
1105	8	-0.06189463432598785
1105	9	0.10832419378219527
1105	10-11	0.008631528183173032
1105	12-13	-0.07384394550931717
1105	14-15	-0.1173570363750116
1105	16-17	-0.04941029609330627
1105	18-19	-0.07150181884818352
1105	20-21	-0.03346667999347375
1105	22-23	-0.08159232119054138
1105	24-25	-0.16283641315632735
1105	26-27	-0.032038440396569
1105	28-29	-0.201879269569325
1105	30-31	-0.034100406871147015
1105	32-33	-0.08433079561516621
1105	34-35	0.018653171086370435
1105	36-37	-0.029508912608456228
1105	38-39	-0.17194410124809423
1105	40-41	0.024480829700081586
1105	42-43	0.05563962094478825
1105	44-45	0.00266979241209242
1105	46-47	-0.19452314069467036
1105	48-49	-0.0800109351267082
1105	50-51	-0.053534107534090936
1105	52-53	-0.0772100847091437
1105	54-55	-0.004199348659824409
1105	56-57	0.15913303325189787
1105	58-59	0.09429003240421707
1105	60-61	-0.0501624112979826
1105	62-63	-0.2846809897536744
1105	64-65	-0.17844084658978687
1105	66-67	-0.1432884514784334
1105	68-69	-0.18599507145688676
1105	70-71	-0.4472000173973285
1105	72-73	-0.17766161817865367
1105	74-75	-0.18814785864592665
1105	76-77	-0.29363657980174906
1105	78-79	-0.28796251745101387
1105	80-81	-0.19344917919851667
1105	82-83	-0.06680421811480386
1105	84-85	-0.12925177249033482
1105	86-87	-0.17880454792609157
1105	88-89	0.007660556859924839
1105	90-91	-0.021104619922986956
1105	92-93	0.026839461766130057
1105	94-95	0.09353419063680946
1105	96-97	-0.02359201282746426
1105	98-99	-0.2067952500723962
1105	100	-0.16769347733539064
1106	1	1.285937732193359
1106	2	-0.671898967693938
1106	3	-0.1889666357566817
1106	4	-0.14339726745270198
1106	5	-1.0946453780357466
1106	6	-0.761402180564609
1106	7	-0.28859915216149545
1106	8	-0.2469172701408766
1106	9	-0.3598284722137777
1106	10-11	-0.2912552927423775
1106	12-13	-0.21779515275277816
1106	14-15	-0.17415406856817128
1106	16-17	-0.2943486763750016
1106	18-19	-0.07040775446186842
1106	20-21	-0.14354590534558298
1106	22-23	-0.33877604855472043
1106	24-25	-0.3255910660738337
1106	26-27	-0.3870937723885177
1106	28-29	-0.2694000190663033
1106	30-31	-0.24647779620716648
1106	32-33	-0.2930581598002746
1106	34-35	-0.377011441589687
1106	36-37	-0.4495887667331999
1106	38-39	-0.3787505650307921
1106	40-41	-0.36769095098401294
1106	42-43	-0.21672896557431898
1106	44-45	-0.203347561712647
1106	46-47	-0.2945137091051393
1106	48-49	-0.3120657641005593
1106	50-51	-0.4392955511960608
1106	52-53	-0.4163103110672921
1106	54-55	-0.4631656464465479
1106	56-57	-0.40192393354890754
1106	58-59	-0.45043142131811464
1106	60-61	-0.17929973524164922
1106	62-63	-0.3428801849247023
1106	64-65	-0.5004353133905894
1106	66-67	-0.5260915198888867
1106	68-69	-0.7404939396661376
1106	70-71	-0.8411732316830438
1106	72-73	-0.6575660447380116
1106	74-75	-0.6775310326901902
1106	76-77	-0.504913617025089
1106	78-79	-0.5784837699661054
1106	80-81	-0.5015006128001289
1106	82-83	-0.4806749424608796
1106	84-85	-0.8623566517659853
1106	86-87	-0.42544375818766156
1106	88-89	-0.5259536282507398
1106	90-91	-0.7407914911503539
1106	92-93	-0.4790615694209919
1106	94-95	-0.5645644009124879
1106	96-97	-0.5388774756041315
1106	98-99	-0.8789060398108219
1106	100	-0.8430015759269409
1107	1	1.445770586724569
1107	2	-0.43431245685559894
1107	3	-0.16267060546156564
1107	4	-0.3288365139922682
1107	5	-1.3366880131885281
1107	6	-0.4730076911417811
1107	7	-0.44788029606329616
1107	8	-0.44194860991627394
1107	9	-0.16892108777920356
1107	10-11	-0.15331294487087632
1107	12-13	-0.2565144658893601
1107	14-15	-0.24394138248825215
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2221	3	-0.11848956498056751
2221	4	-0.2983666252480166
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2221	8	0.4052808905501095
2221	9	0.1843745586070753
2221	10-11	0.08373422133206532
2221	12-13	0.23319401955816232
2221	14-15	0.1922216460282371
2221	16-17	0.2533350991795871
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2221	60-61	0.23468596506283035
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2221	64-65	0.2652673601320572
2221	66-67	0.25744868392643383
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2221	76-77	0.41479691317437073
2221	78-79	0.468605900232113
2221	80-81	0.1586869493386871
2221	82-83	0.19294052699788722
2221	84-85	0.2633194962486769
2221	86-87	0.21459665146249307
2221	88-89	0.3568679012962761
2221	90-91	0.30931977617083817
2221	92-93	0.10963897196346295
2221	94-95	0.4340735938678506
2221	96-97	0.43662798484451315
2221	98-99	0.47866936176128405
2221	100	0.5071873649169021
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2222	2	1.9011677480322486
2222	3	0.5163670211607094
2222	4	0.8077744852246944
2222	5	3.224632272743989
2222	6	0.32184999579914475
2222	7	0.4111933887040422
2222	8	0.7072178209845745
2222	9	0.35307646453075847
2222	10-11	0.42535826960496337
2222	12-13	0.35694376188366306
2222	14-15	0.40474166168798575
2222	16-17	0.42699064848252277
2222	18-19	0.33050721860758614
2222	20-21	0.2973393971533156
2222	22-23	0.398142772294527
2222	24-25	0.4411494182062441
2222	26-27	0.40567275388442425
2222	28-29	0.5839632653640834
2222	30-31	0.41999768838606144
2222	32-33	0.3967074505210064
2222	34-35	0.44292019475402356
2222	36-37	0.38732183403713805
2222	38-39	0.4637312920807588
2222	40-41	0.34661453063431935
2222	42-43	0.6298594081026323
2222	44-45	0.4771095921045969
2222	46-47	0.48326086110217403
2222	48-49	0.49563742842151726
2222	50-51	0.4616792131616734
2222	52-53	0.5373265845611854
2222	54-55	0.5684971189445776
2222	56-57	0.6717564502113476
2222	58-59	0.5680348881013089
2222	60-61	0.6763269087741364
2222	62-63	0.4394572140857633
2222	64-65	0.565324339330644
2222	66-67	0.6789086366840422
2222	68-69	0.7349784212970931
2222	70-71	0.7534795091261302
2222	72-73	0.7166127302353757
2222	74-75	0.7169508479072917
2222	76-77	0.8228603660337157
2222	78-79	0.7565137052953688
2222	80-81	0.6482227424012237
2222	82-83	0.5553137373161547
2222	84-85	0.5594112914919833
2222	86-87	0.5283311173368546
2222	88-89	0.6336765856278888
2222	90-91	0.533790263405912
2222	92-93	0.6083616119844066
2222	94-95	0.8397566205693181
2222	96-97	0.8849343315606504
2222	98-99	0.7598689531433891
2222	100	0.7116797590138759
2223	1	1.3835849382505518
2223	2	-0.9760120551102442
2223	3	-0.037462505852420946
2223	4	-0.050891909527665646
2223	5	-1.1127782389330605
2223	6	0.17401253065496647
2223	7	0.4734784712663256
2223	8	0.2702609414854322
2223	9	0.2191471472718618
2223	10-11	0.2595241484690618
2223	12-13	0.32241314147162115
2223	14-15	0.08089470028233592
2223	16-17	0.14547830566617392
2223	18-19	0.08921793385551524
2223	20-21	0.1014072812509994
2223	22-23	0.15287174804748815
2223	24-25	0.06834616798536075
2223	26-27	0.14156897556217274
2223	28-29	0.11695438225401489
2223	30-31	0.06753366662369586
2223	32-33	0.28841124136360463
2223	34-35	0.14211566638227424
2223	36-37	0.10962090021505588
2223	38-39	0.05990148662430528
2223	40-41	0.14893484683840086
2223	42-43	0.2965871402701197
2223	44-45	0.3560872749684023
2223	46-47	0.19776178601406968
2223	48-49	0.12520603522364127
2223	50-51	0.17174050333744617
2223	52-53	0.2793305084996973
2223	54-55	0.2120379155958787
2223	56-57	0.3383920080379781
2223	58-59	0.4566413330464343
2223	60-61	0.2509688258621665
2223	62-63	0.30143141948052943
2223	64-65	0.14424919477534814
2223	66-67	0.38290744763504136
2223	68-69	0.35153575575341733
2223	70-71	0.22986962780634457
2223	72-73	0.40031753576383977
2223	74-75	0.3484186301907286
2223	76-77	0.44992816775057776
2223	78-79	0.5526931940849593
2223	80-81	0.42658733147744954
2223	82-83	0.3397301479589885
2223	84-85	0.34783709393202855
2223	86-87	0.40143787077798265
2223	88-89	0.3438102710672055
2223	90-91	0.3181842065531697
2223	92-93	0.47712282532902606
2223	94-95	0.568930452350763
2223	96-97	0.5738350814949271
2223	98-99	0.4837956645568582
2223	100	0.49928242538711487
2224	1	1.4119529686096541
2224	2	-0.7689032332608967
2224	3	-0.05156737239333786
2224	4	-0.1895757680508261
2224	5	-0.9957955858050589
2224	6	0.4444051291375892
2224	7	0.17047895021211446
2224	8	0.10670452674887798
2224	9	-0.04200339980932455
2224	10-11	-0.013118684419012538
2224	12-13	0.08501799652632513
2224	14-15	0.06607073940261188
2224	16-17	0.030629287485730572
2224	18-19	0.10846253924838578
2224	20-21	0.0980789844737231
2224	22-23	0.08532765115565866
2224	24-25	0.1377818208178141
2224	26-27	0.191566784496203
2224	28-29	0.0762892595649376
2224	30-31	0.0718496804974933
2224	32-33	0.022728372970611588
2224	34-35	0.03587763399426791
2224	36-37	-0.005605714885312807
2224	38-39	0.1456002542413941
2224	40-41	0.09267724929300414
2224	42-43	0.2004471228535607
2224	44-45	0.24738887819165
2224	46-47	0.08121869739867549
2224	48-49	0.07261813535168926
2224	50-51	0.08313379543240984
2224	52-53	0.09551532404572072
2224	54-55	0.08492645674554922
2224	56-57	0.2034559340768496
2224	58-59	0.24007936458504275
2224	60-61	0.18362308318057075
2224	62-63	0.24870240337725846
2224	64-65	0.33213232968690676
2224	66-67	0.13643366427027104
2224	68-69	0.22237270627606165
2224	70-71	0.4270838080764463
2224	72-73	0.36276150673211305
2224	74-75	0.24831017725124838
2224	76-77	0.47421569969719
2224	78-79	0.3721740678164238
2224	80-81	0.3482940772773091
2224	82-83	0.2728386412449737
2224	84-85	0.0975969936410479
2224	86-87	0.2419422638169948
2224	88-89	0.36597661949843285
2224	90-91	0.3061004294565848
2224	92-93	0.20342943904718425
2224	94-95	0.4441095744070367
2224	96-97	0.3174762123011803
2224	98-99	0.26368204572087706
2224	100	0.4284795280836349
2225	1	1.031779112792698
2225	2	1.8088829929315668
2225	3	0.7556357254669184
2225	4	1.1165349408209249
2225	5	3.2425785488223937
2225	6	0.07105296241713788
2225	7	0.1936535112239639
2225	8	0.37296778374984996
2225	9	0.1879810489998519
2225	10-11	0.27245997421918133
2225	12-13	0.2115009191430559
2225	14-15	0.19691101304668024
2225	16-17	0.25487722855304185
2225	18-19	0.20373852147109517
2225	20-21	0.1818234252257227
2225	22-23	0.26786693708480414
2225	24-25	0.18028639093818555
2225	26-27	0.23790408600036983
2225	28-29	0.3438465270990463
2225	30-31	0.15983538132071828
2225	32-33	0.09145604492216819
2225	34-35	0.17060426381807758
2225	36-37	0.1189191338648925
2225	38-39	0.015776353074002714
2225	40-41	0.12885774535888572
2225	42-43	0.3037959433098578
2225	44-45	0.3734184886891967
2225	46-47	0.350785357152418
2225	48-49	0.20794914930047526
2225	50-51	0.26452245469143065
2225	52-53	0.1197381633268293
2225	54-55	0.2022615465632427
2225	56-57	0.3780686486889593
2225	58-59	0.4693131623526767
2225	60-61	0.30517338948578754
2225	62-63	0.33907459982391686
2225	64-65	0.3830795377090297
2225	66-67	0.49285356141445646
2225	68-69	0.4142642335006457
2225	70-71	0.27183617972434604
2225	72-73	0.5983782889483606
2225	74-75	0.4222913109332609
2225	76-77	0.5193604741530322
2225	78-79	0.389338230999563
2225	80-81	0.3913706452211798
2225	82-83	0.25546026718338055
2225	84-85	0.31861610694063813
2225	86-87	0.4739553252027804
2225	88-89	0.26338056432293655
2225	90-91	0.2575705657308376
2225	92-93	0.4251043509266452
2225	94-95	0.7687680238585557
2225	96-97	0.5748874377969031
2225	98-99	0.7312375065414258
2225	100	0.6369223774092987
2226	1	1.6670991519851572
2226	2	1.208850906882759
2226	3	0.8594702763215825
2226	4	1.0176090193903935
2226	5	3.106529681540323
2226	6	-0.1220615935835454
2226	7	0.1935484083552481
2226	8	0.3362164311008158
2226	9	0.3472120865470387
2226	10-11	0.29888721948639585
2226	12-13	0.299546970663755
2226	14-15	0.4013456649073035
2226	16-17	0.3335047037830279
2226	18-19	0.22451299368813693
2226	20-21	0.27844981458417806
2226	22-23	0.3023045907905697
2226	24-25	0.28922436569589394
2226	26-27	0.18099030825866436
2226	28-29	0.38906507406050395
2226	30-31	0.29763730319080395
2226	32-33	0.340676005507639
2226	34-35	0.3831550970406923
2226	36-37	0.39455925628197264
2226	38-39	0.38138500972150524
2226	40-41	0.35306934940479096
2226	42-43	0.47502660792373064
2226	44-45	0.30423466894080775
2226	46-47	0.40235286401664894
2226	48-49	0.2959193889158769
2226	50-51	0.30787987681096496
2226	52-53	0.3583351721259831
2226	54-55	0.4699214130041014
2226	56-57	0.5210130490799614
2226	58-59	0.5108431538104412
2226	60-61	0.32586104175572217
2226	62-63	0.34635771172649754
2226	64-65	0.5245964343831417
2226	66-67	0.6543941818544923
2226	68-69	0.6352634995035515
2226	70-71	0.5951617034103975
2226	72-73	0.42618379848286025
2226	74-75	0.7782057116344063
2226	76-77	0.7713267018636571
2226	78-79	0.6676126256817057
2226	80-81	0.4701017874883391
2226	82-83	0.4736701911149339
2226	84-85	0.5369292192988553
2226	86-87	0.6919816541414434
2226	88-89	0.5581266269856897
2226	90-91	0.4337432667826704
2226	92-93	0.4250548625995805
2226	94-95	0.7628006122569602
2226	96-97	0.6237883183253814
2226	98-99	0.5796288200286384
2226	100	0.5538020895691034
2227	1	1.3989536052092326
2227	2	1.8582002386552698
2227	3	0.5599619356718897
2227	4	-0.17769290237333735
2227	5	3.232755415615049
2227	6	0.28982797816555106
2227	7	0.41850402244168095
2227	8	0.3351620949384895
2227	9	0.3594274801671702
2227	10-11	0.23170899297191028
2227	12-13	0.12268500597738097
2227	14-15	0.13676855047945224
2227	16-17	0.18242910140277502
2227	18-19	0.14634422966511806
2227	20-21	0.17527353909886045
2227	22-23	0.15526859430241302
2227	24-25	0.20379484662458225
2227	26-27	0.19320979904005497
2227	28-29	0.16132319521941696
2227	30-31	0.2287612910944219
2227	32-33	0.28725136400924356
2227	34-35	0.2363288361880933
2227	36-37	0.05459476501282978
2227	38-39	0.10134169687398042
2227	40-41	0.30681103314296365
2227	42-43	0.4904545661717066
2227	44-45	0.26946791447782203
2227	46-47	0.33651109248216216
2227	48-49	0.21175764859785318
2227	50-51	0.28375305991504973
2227	52-53	0.26836131591683454
2227	54-55	0.2947609408550491
2227	56-57	0.5158012648637893
2227	58-59	0.48584734852315137
2227	60-61	0.32674193142501906
2227	62-63	0.4605682277737131
2227	64-65	0.36938016279987806
2227	66-67	0.46310689280952744
2227	68-69	0.2740645920798883
2227	70-71	0.49546664926933914
2227	72-73	0.4692037965318292
2227	74-75	0.3814243244526665
2227	76-77	0.6460893591653871
2227	78-79	0.5087334919386493
2227	80-81	0.3405122840252659
2227	82-83	0.300202041666104
2227	84-85	0.33546477680543774
2227	86-87	0.26702350371709827
2227	88-89	0.2710562923841806
2227	90-91	0.46280969932583815
2227	92-93	0.40928664486472144
2227	94-95	0.40013004353195214
2227	96-97	0.3984737148720612
2227	98-99	0.5130989205066356
2227	100	0.5614567574063898
2228	1	1.650768534386314
2228	2	1.4447100656414964
2228	3	-0.07439183583230147
2228	4	-0.23000775803181917
2228	5	3.0729593071996177
2228	6	-0.07180217426301283
2228	7	0.13312638189862014
2228	8	0.33580533367788945
2228	9	0.4004185108979499
2228	10-11	0.4717610441408553
2228	12-13	0.27019203925680557
2228	14-15	0.21634214259825768
2228	16-17	0.2365842746616167
2228	18-19	0.08262193392429396
2228	20-21	0.04753399099318756
2228	22-23	0.169724703698094
2228	24-25	0.23637162454939897
2228	26-27	0.13930419692271556
2228	28-29	0.1934775842798402
2228	30-31	0.1512405343429677
2228	32-33	0.20534517374310468
2228	34-35	0.36248665611653763
2228	36-37	0.31010022989544694
2228	38-39	0.3259298456756241
2228	40-41	0.24631414543037522
2228	42-43	0.5491466212616416
2228	44-45	0.36398989653928737
2228	46-47	0.30662244865527555
2228	48-49	0.21172216362015206
2228	50-51	0.32780648343120333
2228	52-53	0.3805269778292981
2228	54-55	0.16579510249794538
2228	56-57	0.46641774283271786
2228	58-59	0.499301056311225
2228	60-61	0.34533772616108394
2228	62-63	0.43142467045370836
2228	64-65	0.3827931902958355
2228	66-67	0.5560127399888515
2228	68-69	0.4766181381270371
2228	70-71	0.5013002031584719
2228	72-73	0.4972538872047707
2228	74-75	0.44192366278526407
2228	76-77	0.6035526733838807
2228	78-79	0.4538033346088497
2228	80-81	0.45411400926175105
2228	82-83	0.36221331451812944
2228	84-85	0.45472106264575984
2228	86-87	0.38579956606086796
2228	88-89	0.419231756776675
2228	90-91	0.44847945377370735
2228	92-93	0.5363841305285888
2228	94-95	0.8351571473892321
2228	96-97	0.5808660101227616
2228	98-99	0.7395457175462852
2228	100	0.7047639181180543
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	33.0
14	135.0
15	445.0
16	1713.0
17	4128.0
18	7027.0
19	8758.0
20	10453.0
21	12038.0
22	13600.0
23	16030.0
24	18182.0
25	20238.0
26	23138.0
27	25943.0
28	29643.0
29	32955.0
30	37826.0
31	43756.0
32	51339.0
33	61285.0
34	74781.0
35	97300.0
36	136304.0
37	225070.0
38	554691.0
39	1016964.0
40	1019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.715546803381663	22.5581970918877	20.23495953410161	31.49129657062903
2	21.964199705942	47.22704573108903	14.243275958223483	16.565478604745486
3	35.54243423176291	18.52786721234404	25.5050175993232	20.42468095656985
4	21.379920017838945	17.408219935496213	21.226126449760834	39.98573359690401
5	17.921881739360497	33.95015789288157	26.93054891589991	21.19741145185802
6	17.656673979419466	28.50127910463072	30.138628088496063	23.703418827453756
7	20.70224606733251	26.664549018085697	35.168663976302405	17.464540938279388
8	21.587341319931415	28.536093564157028	32.37998220066199	17.496582915249565
9	21.258009999156375	28.537004522711186	26.924370240489097	23.280615237643342
10-11	19.591233093302353	30.132469216532076	29.12168148690616	21.154616203259412
12-13	23.860757212514354	26.913498148179688	27.407772377597865	21.817972261708093
14-15	21.93297087744716	27.39517738541429	28.593820611665098	22.07803112547345
16-17	20.831324933767373	29.43952680852008	28.54443081527226	21.18471744244029
18-19	21.379979428179432	29.776720097686443	29.540385763222858	19.302914710911264
20-21	22.650132900931673	29.00551446780415	29.672157898426537	18.672194732837642
22-23	21.433527948406475	30.119577172646057	28.882772704354736	19.564122174592733
24-25	22.106785730269927	28.402558922185523	26.892130229050625	22.598525118493924
26-27	20.208704565447025	26.758912442228393	30.189839801997216	22.842543190327362
28-29	19.858222882690484	27.950917455901596	30.509904119441956	21.680955541965965
30-31	20.08230309228776	30.091409589985783	28.31896983685545	21.50731748087101
32-33	21.03258736389586	29.81703991543136	28.664083241016264	20.486289479656513
34-35	21.37851397311405	28.770487160831316	30.248240166697492	19.60275869935714
36-37	22.14468952750164	28.26359813578273	28.61992155458642	20.97179078212921
38-39	21.325275913490003	29.455539954728998	28.929734889367253	20.289449242413742
40-41	20.85311144746585	29.542972332457285	30.004754866906303	19.59916135317056
42-43	20.740586273702522	29.692901704900226	29.30600138109375	20.2605106403035
44-45	20.659629049755367	30.168135224272053	28.595622725326585	20.576613000645988
46-47	20.07888681542161	28.06706310093666	28.841613042683523	23.012437040958208
48-49	21.036972257736416	28.431879284477418	30.051980107825848	20.479168349960315
50-51	22.08314041475547	30.677182825025064	28.078930994097384	19.160745766122087
52-53	20.301147805931897	30.12519736918975	28.48433730788128	21.08931751699707
54-55	21.902007587304208	28.60318216759691	28.011623593176616	21.483186651922267
56-57	21.235699717133564	28.81191342647077	29.091452490044617	20.860934366351056
58-59	20.900737027157426	28.042821729427136	29.18413240661589	21.872308836799544
60-61	19.776917381127927	30.360509100803647	29.617996165643717	20.244577352424713
62-63	20.153550103425683	31.11827683541967	27.38277168156456	21.345401379590083
64-65	19.906923781468922	30.201628833824294	28.92830655741763	20.963140827289156
66-67	20.204169576123064	30.174333703129697	29.462934482672576	20.158562238074662
68-69	20.8703773309152	29.98839914084726	28.993929451408878	20.147294076828658
70-71	19.562320060931246	30.791527927018752	29.450636542191443	20.19551546985856
72-73	20.26912488172391	30.623990766840954	28.48438676448513	20.622497586950004
74-75	20.136996284477306	29.120394262862238	30.26976651340119	20.472842939259266
76-77	19.729500759066116	30.347811897356358	28.738009508030892	21.184677835546633
78-79	19.50687040980857	29.466697137570186	28.47965374069287	22.54677871192837
80-81	21.158578039817247	29.368956600901335	28.436054903922493	21.03641045535893
82-83	20.266807878444858	28.03465048698656	29.870865683914015	21.827675950654562
84-85	20.578434917754304	29.104591112292276	28.81944128139391	21.49753268855951
86-87	21.539298157922985	28.31011643238529	29.0814012760549	21.069184133636828
88-89	20.70555324295304	28.574274609644355	28.773794336451846	21.94637781095075
90-91	20.708404939296493	29.115067135665097	28.54985695970355	21.626670965334856
92-93	20.43909786586175	30.113814369618662	28.60221727312082	20.84487049139877
94-95	21.009199493190188	29.99099339238193	28.145846840934357	20.85396027349352
96-97	20.02078965848164	30.605791399204612	28.56835337904233	20.80506556327142
98-99	22.320999614624924	30.160372273114884	27.618778103566484	19.89985000869371
100	20.759161173520575	31.43595189029841	27.786612315446728	20.01827462073429
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	9.5
2	15.5
3	16.0
4	25.0
5	32.5
6	33.0
7	38.0
8	44.0
9	57.0
10	61.5
11	72.0
12	91.0
13	103.5
14	151.0
15	176.5
16	210.0
17	302.0
18	449.5
19	693.5
20	1128.0
21	1778.0
22	2738.0
23	4185.0
24	6369.0
25	9508.0
26	13636.0
27	18488.0
28	24054.5
29	30459.0
30	37711.0
31	45818.0
32	55243.0
33	65990.0
34	76860.0
35	88435.5
36	99882.5
37	108764.5
38	115275.0
39	119904.5
40	122009.5
41	121920.0
42	120271.0
43	117463.0
44	115893.0
45	114469.5
46	108090.0
47	97879.0
48	90499.5
49	87401.0
50	83280.5
51	75836.5
52	66689.5
53	57138.0
54	47493.5
55	37677.0
56	28634.0
57	21017.5
58	14780.5
59	10494.5
60	8130.5
61	6805.5
62	5693.5
63	4304.5
64	3221.0
65	3288.0
66	4419.5
67	4416.0
68	2860.0
69	2533.5
70	3695.0
71	3710.0
72	2062.0
73	662.5
74	241.5
75	155.0
76	100.5
77	78.0
78	63.5
79	47.0
80	40.5
81	34.5
82	27.0
83	20.5
84	20.0
85	22.5
86	20.5
87	17.5
88	16.5
89	15.5
90	13.0
91	13.5
92	10.5
93	6.0
94	10.0
95	12.0
96	10.5
97	17.5
98	45.5
99	88.5
100	86.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.342820240548509
2	0.005584572005926776
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.9803446829527575E-4
30-31	0.009584868265491346
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.129240858629927E-4
40-41	0.001247617150260237
42-43	9.901723414763787E-5
44-45	0.0
46-47	0.002237789491736616
48-49	3.960689365905514E-5
50-51	0.0
52-53	0.0015644722995326783
54-55	0.018397402104631116
56-57	0.011882068097716544
58-59	0.013743592099692135
60-61	3.3665859610196877E-4
62-63	0.011684033629421268
64-65	1.980344682952757E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	9.901723414763787E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2524813.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.88950087004956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4354797848638	68.88195379646399
2	7.54379927080613	11.751655197338666
3	1.9011217476735964	4.442322720584782
4	0.75050310385225	2.3382524864189893
5	0.3858496929825264	1.5026819998635423
6	0.2303743213448269	1.0766244541694983
7	0.16180390031265418	0.8821977523925921
8	0.09432800717941643	0.5877729117816958
9	0.07642828216069715	0.5357664674866812
>10	0.3885781673844033	5.37157624020423
>50	0.022275478570354453	1.1692866790860856
>100	0.008943079451388789	1.2034250432050537
>500	5.151634180295826E-4	0.2564842510042267
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	7.921378731811029E-5	0.0	0.0	0.0	0.0
14-15	7.921378731811029E-5	0.0	0.0	0.0	0.0
16-17	7.921378731811029E-5	0.0	0.0	0.0	0.0
18-19	7.921378731811029E-5	0.0	0.0	0.0	0.0
20-21	7.921378731811029E-5	0.0	0.0	0.0	0.0
22-23	7.921378731811029E-5	1.980344682952757E-5	0.0	0.0	0.0
24-25	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
26-27	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
28-29	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
30-31	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
32-33	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
34-35	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
36-37	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
38-39	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
40-41	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
42-43	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
44-45	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
46-47	7.921378731811029E-5	3.960689365905514E-5	0.0	0.0	0.0
48-49	1.1882068097716542E-4	3.960689365905514E-5	1.980344682952757E-5	0.0	0.0
50-51	2.178379151248033E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
52-53	2.5744480878385847E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
54-55	2.7724825561338605E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
56-57	2.970517024429136E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
58-59	3.1685514927244114E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
60-61	4.7528272390866174E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
62-63	4.950861707381894E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
64-65	5.148896175677169E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
66-67	5.941034048858271E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
68-69	6.535137453744099E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
70-71	7.723344263515753E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
72-73	7.921378731811029E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
74-75	9.505654478173235E-4	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
76-77	0.0010693861287944888	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
78-79	0.0013466343844078749	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
80-81	0.0015248654058736232	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
82-83	0.001762506767827954	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
84-85	0.0018813274488051193	3.960689365905514E-5	3.960689365905514E-5	0.0	0.0
86-87	0.0019803446829527575	1.1882068097716544E-4	3.960689365905514E-5	0.0	0.0
88	0.0019803446829527575	1.1882068097716544E-4	3.960689365905514E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	8805	0.0	80.01764	8
CTTCACC	9950	0.0	74.01748	6
CACCGGC	9270	0.0	73.2695	9
CGGGCCC	730	0.0	70.730095	7
CCTTCAC	10985	0.0	70.46201	5
GGCGCGC	500	0.0	67.592255	6
CGTAGTG	2140	0.0	66.89913	4
GTGTGGC	3795	0.0	66.172325	7
TTCACCG	11050	0.0	65.41734	7
GGGCCCG	790	0.0	64.76403	8
AACGGGC	785	0.0	64.57858	5
GGCCCGA	795	0.0	64.356705	9
CGGGGCA	945	0.0	64.07555	94
AGGCGCG	525	0.0	63.479496	5
GCCTTCA	3600	0.0	63.367733	4
GCGCGCA	540	0.0	62.58542	7
AGGCTGC	60	3.6561687E-10	62.58542	2
CGCAGCC	35	1.6313836E-4	60.505695	1
AGGCAGC	70	2.1827873E-11	60.350224	2
CGAGCGA	705	0.0	59.92221	7
>>END_MODULE