FastQCFastQC Report
Tue 7 Mar 2017
1HL_12A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_12A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2524813
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG88050.080.017648
CTTCACC99500.074.017486
CACCGGC92700.073.26959
CGGGCCC7300.070.7300957
CCTTCAC109850.070.462015
GGCGCGC5000.067.5922556
CGTAGTG21400.066.899134
GTGTGGC37950.066.1723257
TTCACCG110500.065.417347
GGGCCCG7900.064.764038
AACGGGC7850.064.578585
GGCCCGA7950.064.3567059
CGGGGCA9450.064.0755594
AGGCGCG5250.063.4794965
GCCTTCA36000.063.3677334
GCGCGCA5400.062.585427
AGGCTGC603.6561687E-1062.585422
CGCAGCC351.6313836E-460.5056951
AGGCAGC702.1827873E-1160.3502242
CGAGCGA7050.059.922217
Produced by FastQC (version 0.11.5)