FastQCFastQC Report
Mon 6 Mar 2017
1HL_13A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_13A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2676425
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA143520.5362377051477251No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG92330.3449751067188507No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG88120.32924516846166063No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA70860.26475615793455826No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA58830.2198081395891908No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC57360.21431573834499376No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG57090.2133069299532025No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT50110.18722736486170918No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG48170.17997888975031992No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG47120.1760557460044649No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA45510.17004025892748723No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA45270.16914354035700607No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA44990.16809736869144476No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA43880.16395004530296944No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC43800.16365113911280907No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG43790.16361377583903902No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT41310.15434768394406717No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA39720.14840692341462958No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT39610.14799592740315906No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA35140.1312945440279477No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA35080.13107036438532743No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC32460.12128118665757494No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA31750.11862839421990154No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG31220.11664814071008901No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30110.11250081732161372No Hit
CAGCTGCCCGCCGACGAGCTGAATTCTCCGGGCCGACTTTGCGGGTCCAC29910.11175355184621276No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT27590.10308527233156169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT450.086.999743
GCTGCCG257.8036464E-786.999743
GCAGCTA450.086.999743
GCAGCCT302.2700988E-886.999743
GCAGCCG402.0008883E-1186.999743
AGCAGCC1800.086.999742
CAGCAGC6750.086.999731
GCTGCAA850.086.999733
CAGCTGC8500.085.9762041
GCTGCCC4250.084.952683
AGCTGCA1800.084.5830842
AGCTGCC5100.084.4409262
AGCAGCG1350.083.777532
TGCTGCC950.082.420812
AGCAGCA2800.082.3390352
CAGCGTA63250.082.2542951
GCAGCCC750.081.199753
CAGCCCT157700.081.151941
CTGCTGC2150.080.929991
GCTGCCT700.080.785473
Produced by FastQC (version 0.11.5)