FastQCFastQC Report
Tue 7 Mar 2017
1HL_13A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_13A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2676425
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG102250.080.379718
GGCGCGC6050.075.255796
CACCGGC107650.073.42639
GGCTGCA800.072.832751
CTTCACC117650.072.730796
GTGTGGC42450.070.6556247
AGGCGCG6550.069.511085
CCTTCAC130950.068.9999545
CGTAGTG23800.068.8290944
GCGCGCA6650.068.46587
TTCACCG128050.065.8340157
AACGGGC7650.065.038135
CGGGGCA10800.064.32229694
CGCAGCA301.3798557E-462.5897062
CGGGCCC8200.062.393057
GGGCCCG8250.062.0149158
TGCTGCG609.95351E-961.7974851
CAGCGTA10750.061.5921751
GCCTTCA38950.060.495054
TGTGGCA51250.060.08058
Produced by FastQC (version 0.11.5)