##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1HL_13A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2676425
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.903554181417375	12.0	12.0	12.0	2.0	32.0
2	14.53135432526598	12.0	12.0	12.0	12.0	22.0
3	13.73898951026089	12.0	12.0	12.0	12.0	22.0
4	13.713014562335951	12.0	12.0	12.0	12.0	22.0
5	22.435855665673426	22.0	22.0	22.0	22.0	27.0
6	26.80739307098088	27.0	22.0	32.0	22.0	32.0
7	34.94919603575665	37.0	37.0	37.0	27.0	41.0
8	37.58019148677807	41.0	37.0	41.0	27.0	41.0
9	38.51396620491887	41.0	37.0	41.0	32.0	41.0
10-11	38.8663086019597	41.0	41.0	41.0	37.0	41.0
12-13	39.05283204274359	41.0	41.0	41.0	37.0	41.0
14-15	39.00293077519453	41.0	41.0	41.0	37.0	41.0
16-17	38.91158504348151	41.0	41.0	41.0	37.0	41.0
18-19	38.83750581465948	41.0	41.0	41.0	37.0	41.0
20-21	38.85176177176644	41.0	41.0	41.0	37.0	41.0
22-23	38.85022819619455	41.0	41.0	41.0	37.0	41.0
24-25	38.87203975452329	41.0	41.0	41.0	37.0	41.0
26-27	38.83507458643527	41.0	41.0	41.0	37.0	41.0
28-29	38.783199977582036	41.0	41.0	41.0	34.5	41.0
30-31	38.66771663693173	41.0	41.0	41.0	32.0	41.0
32-33	38.70683710546718	41.0	41.0	41.0	32.0	41.0
34-35	38.67488478100451	41.0	41.0	41.0	32.0	41.0
36-37	38.59837077444726	41.0	41.0	41.0	32.0	41.0
38-39	38.50116984410174	41.0	41.0	41.0	32.0	41.0
40-41	38.54381703204835	41.0	41.0	41.0	32.0	41.0
42-43	38.2852818591965	41.0	41.0	41.0	32.0	41.0
44-45	38.394715338557965	41.0	41.0	41.0	32.0	41.0
46-47	38.41259889591526	41.0	41.0	41.0	32.0	41.0
48-49	38.34834396629833	41.0	41.0	41.0	32.0	41.0
50-51	38.23985073372128	41.0	41.0	41.0	32.0	41.0
52-53	38.2078193859346	41.0	41.0	41.0	32.0	41.0
54-55	38.07473177839842	41.0	37.0	41.0	32.0	41.0
56-57	37.861926450395586	41.0	37.0	41.0	27.0	41.0
58-59	37.86103328133611	41.0	37.0	41.0	29.5	41.0
60-61	37.86603472916297	41.0	37.0	41.0	29.5	41.0
62-63	37.866800863091626	41.0	37.0	41.0	29.5	41.0
64-65	37.848220107045776	41.0	37.0	41.0	29.5	41.0
66-67	37.719957592684274	41.0	37.0	41.0	27.0	41.0
68-69	37.73509457578673	41.0	37.0	41.0	27.0	41.0
70-71	37.713234632018455	41.0	37.0	41.0	27.0	41.0
72-73	37.57003428080368	41.0	37.0	41.0	27.0	41.0
74-75	37.47401739260394	41.0	37.0	41.0	27.0	41.0
76-77	36.4359361835284	39.0	34.5	41.0	24.5	41.0
78-79	36.94653446294964	41.0	37.0	41.0	27.0	41.0
80-81	37.59990304230457	41.0	37.0	41.0	27.0	41.0
82-83	37.80349664197577	41.0	37.0	41.0	27.0	41.0
84-85	37.70487310498146	41.0	37.0	41.0	27.0	41.0
86-87	37.575270930438926	41.0	37.0	41.0	27.0	41.0
88-89	37.49628870601642	41.0	37.0	41.0	27.0	41.0
90-91	37.34360592955154	41.0	37.0	41.0	27.0	41.0
92-93	37.23220247158056	41.0	37.0	41.0	27.0	41.0
94-95	37.07917987614075	41.0	37.0	41.0	27.0	41.0
96-97	37.01051028891151	41.0	37.0	41.0	27.0	41.0
98-99	36.89534322837367	41.0	37.0	41.0	27.0	41.0
100	36.76714236341388	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.435678981627051
1101	2	-0.46957238904178134
1101	3	0.10173580047551845
1101	4	0.03958996965438644
1101	5	-1.0501940134792846
1101	6	-0.7028759736786867
1101	7	-0.2161761001003839
1101	8	-0.018662026750696725
1101	9	-0.0835733388294031
1101	10-11	-0.014502034487478
1101	12-13	0.08585770540355497
1101	14-15	0.10651678817328047
1101	16-17	0.006834125121180534
1101	18-19	0.09342461139674896
1101	20-21	0.1612262307724066
1101	22-23	0.15224045885429405
1101	24-25	0.07785425226832388
1101	26-27	0.18553282910658453
1101	28-29	0.23661897295735912
1101	30-31	0.12432279920979994
1101	32-33	-0.03330345389159106
1101	34-35	0.12411350892794815
1101	36-37	0.14142279150300396
1101	38-39	0.057080896379211765
1101	40-41	0.17692931622011798
1101	42-43	0.11980602255121653
1101	44-45	0.10679979341745849
1101	46-47	0.05737170552350079
1101	48-49	-0.010027443757145704
1101	50-51	0.08118902945803086
1101	52-53	0.1797004591668454
1101	54-55	0.03782242707679728
1101	56-57	0.18380243711398947
1101	58-59	0.2806834872986599
1101	60-61	-0.11830052372298638
1101	62-63	-0.008544570064877632
1101	64-65	0.06258235153823932
1101	66-67	0.03362540336736686
1101	68-69	0.16085082507618864
1101	70-71	0.2544649813633342
1101	72-73	0.13258911178874655
1101	74-75	0.25241274367573396
1101	76-77	0.02378625765849307
1101	78-79	0.15806880336151607
1101	80-81	0.1367791882246152
1101	82-83	0.04491091929807567
1101	84-85	-0.0031845695020038534
1101	86-87	-0.16626219270636255
1101	88-89	-0.19866958307706284
1101	90-91	-0.1134417444931799
1101	92-93	0.10365472974040557
1101	94-95	0.09175471719002815
1101	96-97	-0.08841858938203728
1101	98-99	-0.22549149122913548
1101	100	-0.3637603375924314
1102	1	1.7670913463896802
1102	2	0.6329416230771923
1102	3	-0.04374693389448758
1102	4	-0.05841849799875298
1102	5	-0.8259574874902675
1102	6	-0.22252987966784588
1102	7	-0.09203476417182088
1102	8	-0.21058188240278497
1102	9	-0.1305432403005895
1102	10-11	-0.16749803508419348
1102	12-13	-0.2119447539097905
1102	14-15	-0.35585934043275813
1102	16-17	-0.2470645827092639
1102	18-19	-0.19773283580473588
1102	20-21	-0.07760349978289582
1102	22-23	-0.08847993099873008
1102	24-25	-0.16381519669489109
1102	26-27	-0.24755086710926122
1102	28-29	-0.19500028811204118
1102	30-31	-0.2312816931927486
1102	32-33	-0.2245010372006533
1102	34-35	-0.13318185020141726
1102	36-37	-0.32835596264380484
1102	38-39	-0.2641095476753108
1102	40-41	-0.31394078486604116
1102	42-43	-0.17943973829441262
1102	44-45	-0.08376573499393203
1102	46-47	-0.410932313059881
1102	48-49	-0.36897957168121565
1102	50-51	-0.19294147327335764
1102	52-53	-0.17021092172836916
1102	54-55	-0.2860448822411712
1102	56-57	-0.11939726310836107
1102	58-59	-0.13861363355719902
1102	60-61	-0.19474364766186625
1102	62-63	-0.27482402392966776
1102	64-65	-0.21541595777262046
1102	66-67	-0.1551750242775327
1102	68-69	-0.3363520789310357
1102	70-71	-0.37383861099588245
1102	72-73	-0.3405365675059926
1102	74-75	-0.4657536400254543
1102	76-77	-0.23533174249779165
1102	78-79	-0.3934878027576403
1102	80-81	-0.31769829648459336
1102	82-83	-0.35537234216912594
1102	84-85	-0.362602347311352
1102	86-87	-0.301294088480347
1102	88-89	-0.4078245226597943
1102	90-91	-0.44816338460371696
1102	92-93	-0.4281598840649963
1102	94-95	-0.22222596775218761
1102	96-97	-0.2665587958166
1102	98-99	-0.37730809408058263
1102	100	-0.5749497871239413
1103	1	1.6047730772782725
1103	2	0.7528310693593312
1103	3	0.37138264178077485
1103	4	0.9586611021385263
1103	5	2.7829696887165873
1103	6	-0.06605401674277545
1103	7	-0.5273692366319693
1103	8	-0.08272037393600584
1103	9	-0.07491770633489381
1103	10-11	-0.24707224992643262
1103	12-13	-0.507756089682033
1103	14-15	-0.2245317100355919
1103	16-17	-0.2811195455781146
1103	18-19	-0.30708043012954533
1103	20-21	-0.1670589095399535
1103	22-23	-0.2206987820870978
1103	24-25	-0.38769396001208634
1103	26-27	-0.21888558053854723
1103	28-29	-0.23520781365111532
1103	30-31	-0.18275215256755217
1103	32-33	-0.2650740325930343
1103	34-35	-0.35616446391329504
1103	36-37	-0.2677708457737751
1103	38-39	-0.1296133557178436
1103	40-41	-0.3241551378639187
1103	42-43	-0.20574279714200117
1103	44-45	-0.6063469160347736
1103	46-47	-0.6565464922678927
1103	48-49	-0.3837144691232979
1103	50-51	-0.10681782689920993
1103	52-53	-0.23276431829784627
1103	54-55	-0.44726966263066714
1103	56-57	-0.4134228715554187
1103	58-59	-0.5481753618170302
1103	60-61	-0.31223928466011586
1103	62-63	-0.21601678463270702
1103	64-65	-0.2723598849853346
1103	66-67	-0.43256244422831003
1103	68-69	-0.3493488952176236
1103	70-71	-0.5243267560446938
1103	72-73	-0.3777498690021872
1103	74-75	-0.45179203444081395
1103	76-77	-0.5229660097690711
1103	78-79	-0.5565382894422726
1103	80-81	-0.21878137972117173
1103	82-83	-0.5408050278066412
1103	84-85	-0.4926037347093981
1103	86-87	-0.2666564184328095
1103	88-89	-0.306367207055942
1103	90-91	-0.6591888178004979
1103	92-93	-0.40424534048244
1103	94-95	-0.3972288885901776
1103	96-97	-0.20580481800036665
1103	98-99	-0.16414168663828121
1103	100	-0.3196999459445067
1104	1	1.9161851526203044
1104	2	-0.5505987473493832
1104	3	-0.026170460245648286
1104	4	0.14308495984589342
1104	5	-1.1709271726349044
1104	6	-0.4975661924564534
1104	7	-0.3398415440043152
1104	8	-0.31832246817684506
1104	9	-0.20370537961161972
1104	10-11	-0.25857881929589865
1104	12-13	-0.23772358192809406
1104	14-15	-0.3083318619494406
1104	16-17	-0.1653349738756802
1104	18-19	-0.2999685112226018
1104	20-21	-0.15381844258573807
1104	22-23	-0.13070339375734363
1104	24-25	-0.2420228263550328
1104	26-27	-0.18270092383669834
1104	28-29	-0.12794465908783081
1104	30-31	-0.19689799889946613
1104	32-33	-0.2615305065642275
1104	34-35	-0.1806406776415983
1104	36-37	-0.2762617445069182
1104	38-39	-0.12623711576630825
1104	40-41	-0.29869826602313054
1104	42-43	-0.3119615440336432
1104	44-45	-0.13138896361635943
1104	46-47	-0.41566923503615527
1104	48-49	-0.5688278167437559
1104	50-51	-0.2754365412378874
1104	52-53	-0.25727851048694106
1104	54-55	-0.3412068987029713
1104	56-57	-0.37541615298486164
1104	58-59	-0.29623913850367956
1104	60-61	-0.4682324214097022
1104	62-63	-0.22122978050029474
1104	64-65	-0.3771001846534787
1104	66-67	-0.6759229954585493
1104	68-69	-0.6642457203991512
1104	70-71	-0.47553573704642105
1104	72-73	-0.2811082500346629
1104	74-75	-0.4232234155004164
1104	76-77	-0.5384320597378789
1104	78-79	-0.5070001346529196
1104	80-81	-0.4219548418195771
1104	82-83	-0.2680642940989415
1104	84-85	-0.4079095065311904
1104	86-87	-0.4108389320643653
1104	88-89	-0.433541926060542
1104	90-91	-0.46762252278019645
1104	92-93	-0.4700464147303265
1104	94-95	-0.3560945985613273
1104	96-97	-0.4230065740520601
1104	98-99	-0.4466935227160107
1104	100	-0.3939885279915458
1105	1	1.842901216098861
1105	2	0.4211625127287739
1105	3	-0.10445232379410108
1105	4	-0.26163045677826524
1105	5	-1.1385564150844694
1105	6	0.6474757119870915
1105	7	0.3556052594568655
1105	8	-0.017421755985807863
1105	9	0.04420211152668685
1105	10-11	0.12243488258526014
1105	12-13	0.07147607684535728
1105	14-15	0.06660763111641188
1105	16-17	0.09833027752565471
1105	18-19	0.030333415665488417
1105	20-21	0.11879794598418414
1105	22-23	0.1319716783987559
1105	24-25	0.13910157879578833
1105	26-27	0.18217307341235767
1105	28-29	0.023162862005484897
1105	30-31	0.1152881157268979
1105	32-33	0.023470629410503818
1105	34-35	-0.09250115823849825
1105	36-37	0.03958553707661139
1105	38-39	0.01476837019571775
1105	40-41	0.01708597619850849
1105	42-43	0.1252384085014242
1105	44-45	0.0873192739369344
1105	46-47	0.02955381034566784
1105	48-49	-0.03346029274177198
1105	50-51	0.20363131552510794
1105	52-53	-0.029015061305891265
1105	54-55	0.2681407081166398
1105	56-57	0.09015096973586623
1105	58-59	0.11383950784856722
1105	60-61	0.2895253091877379
1105	62-63	-0.12140645351249901
1105	64-65	-0.01042825415487414
1105	66-67	0.11929503933411922
1105	68-69	-0.04639015126495849
1105	70-71	-0.20775085882363697
1105	72-73	-0.004912817521329771
1105	74-75	-0.08829239159646818
1105	76-77	-0.28810600966671274
1105	78-79	-0.17036867596730332
1105	80-81	-0.20426219823273328
1105	82-83	-0.20296959577258633
1105	84-85	-0.175339663983074
1105	86-87	-0.1947746395125236
1105	88-89	0.00763429979727448
1105	90-91	-0.19214657107576727
1105	92-93	-0.08660556175078682
1105	94-95	-0.055702452126226376
1105	96-97	-0.0316886045960274
1105	98-99	-0.1850283465639606
1105	100	-0.1310910544138082
1106	1	1.2742384588029765
1106	2	-0.6380546526902116
1106	3	-0.09802875622525065
1106	4	-0.25189569099343423
1106	5	-1.2090988027819165
1106	6	-0.9118776686442587
1106	7	-0.383902768171275
1106	8	-0.25483370880964884
1106	9	-0.30725790276805753
1106	10-11	-0.19509149799340975
1106	12-13	-0.27839025434701625
1106	14-15	-0.17547409661571578
1106	16-17	-0.23783482647404952
1106	18-19	-0.18340168219842923
1106	20-21	-0.19573047904355434
1106	22-23	-0.2156123067386062
1106	24-25	-0.42141277054373916
1106	26-27	-0.23839357489461577
1106	28-29	-0.37173707300886605
1106	30-31	-0.28688058393582594
1106	32-33	-0.26473145885067595
1106	34-35	-0.1579192552230353
1106	36-37	-0.40666796902620916
1106	38-39	-0.3545451679220406
1106	40-41	-0.3158739716455372
1106	42-43	-0.15094970732301505
1106	44-45	-0.17890298115212033
1106	46-47	-0.43164901009407686
1106	48-49	-0.38419109346049396
1106	50-51	-0.38589608919662766
1106	52-53	-0.29058480820372523
1106	54-55	-0.3627992605660708
1106	56-57	-0.5231302053217846
1106	58-59	-0.37629997583124464
1106	60-61	-0.4731782854636961
1106	62-63	-0.4941541498010622
1106	64-65	-0.6351589195304257
1106	66-67	-0.5746755102742043
1106	68-69	-0.6059426223763467
1106	70-71	-0.7598943386147781
1106	72-73	-0.3261883430486847
1106	74-75	-0.48802674577066796
1106	76-77	-0.5946620077230804
1106	78-79	-0.5210237821890118
1106	80-81	-0.5981262174291331
1106	82-83	-0.5467301367545332
1106	84-85	-0.7647605906162696
1106	86-87	-0.6293738964506943
1106	88-89	-0.6952103831177823
1106	90-91	-0.7127131496182244
1106	92-93	-0.5370943842548641
1106	94-95	-0.5481903837374702
1106	96-97	-0.5975498806517621
1106	98-99	-0.6493260080225909
1106	100	-0.8392974890041742
1107	1	1.1796983142835433
1107	2	-0.6604745512393002
1107	3	-0.17423374732005215
1107	4	-0.19104466930157216
1107	5	-1.1947943496252087
1107	6	-0.31418165317975877
1107	7	-0.26888659655549674
1107	8	-0.2546708768870616
1107	9	-0.13023136011864977
1107	10-11	-0.0505047702944168
1107	12-13	-0.37120619360477747
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2225	1	1.721336855247067
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2227	1	1.2712754468080902
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2227	3	0.7344957439297808
2227	4	-0.10653565281046795
2227	5	3.297473474852829
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2227	10-11	0.33333878499949066
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2227	14-15	0.2579481909479995
2227	16-17	0.26255884825307163
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2227	98-99	0.6492609435763299
2227	100	0.6848828488593242
2228	1	1.2868562807070365
2228	2	1.6751391779129658
2228	3	-0.09156767912830688
2228	4	-0.19147632793698044
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2228	6	0.15342485987834564
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2228	8	0.44646192607366686
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2228	10-11	0.21687645479062923
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2228	32-33	0.24361989790712357
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2228	60-61	0.5082337650691429
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2228	66-67	0.5723811861474957
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2228	74-75	0.5827063189067871
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2228	78-79	0.6809350577236586
2228	80-81	0.5418173101240527
2228	82-83	0.5710012369980078
2228	84-85	0.5591880524549566
2228	86-87	0.5938069914815003
2228	88-89	0.6763631003072277
2228	90-91	0.6166489322882001
2228	92-93	0.6638924730355171
2228	94-95	0.9051356433412607
2228	96-97	0.689898207611435
2228	98-99	0.8140363166657707
2228	100	1.0598091760439488
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	22.0
13	34.0
14	133.0
15	477.0
16	1740.0
17	4483.0
18	7498.0
19	9344.0
20	11044.0
21	12514.0
22	14637.0
23	16789.0
24	18994.0
25	21581.0
26	24162.0
27	27151.0
28	30879.0
29	35172.0
30	39601.0
31	46588.0
32	53982.0
33	64404.0
34	79150.0
35	102591.0
36	144798.0
37	238383.0
38	584784.0
39	1084290.0
40	1200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.67161625708486	17.69350406814785	23.114259986440207	33.52061968832707
2	20.522282654996616	50.80175558953343	12.05745946196397	16.618502293505994
3	39.01136777604454	15.215445977376538	24.982018924498163	20.79116732208076
4	18.00364291919258	15.968577486759388	23.37468825018448	42.65309134386355
5	17.34660974994629	34.696021745425334	26.204433152432816	21.75293535219556
6	17.670063610973592	28.308807457709445	29.55864632859131	24.46248260272565
7	20.166079751907862	26.19845502862961	35.92673062013694	17.70873459932559
8	21.82527065021437	27.89687736439467	33.14129856057988	17.136553424811083
9	21.25955332206208	28.029404896457027	26.908805589545754	23.802236191935137
10-11	19.858150331132013	29.975190786216686	28.491644637903175	21.675014244748127
12-13	24.457307789308498	26.61793250324593	27.001746733048748	21.923012974396816
14-15	21.99910328142952	27.33723156823001	28.41105205638118	22.25261309395929
16-17	21.01443156449368	29.58623910627049	28.21590367748022	21.18342565175561
18-19	21.451750002335203	29.655641387298353	29.512932363133658	19.379676247232783
20-21	22.8330328703401	28.96511204311722	29.641555433087046	18.560299653455637
22-23	21.8233651232521	30.321903285165845	28.549109352961505	19.305622238620547
24-25	22.385551622033123	28.13166817676565	26.477708136786944	23.005072064414282
26-27	20.19965065339025	26.477166369317278	30.034673118058603	23.288509859233866
28-29	19.76577267294297	27.767776844938584	30.470382604803365	21.99606787731508
30-31	20.07784247212625	30.133989792890898	28.104637441362186	21.683530293620667
32-33	21.094351607087813	29.849463369980477	28.426800676275256	20.629384346656455
34-35	21.52602819059006	28.81586444604276	30.026789467293124	19.631317896074055
36-37	22.095855478857057	27.972948989790485	28.661759623378202	21.26943590797426
38-39	21.71132779142694	29.213470131749958	28.864213080615325	20.210988996207774
40-41	20.98901164595896	29.67077906380225	29.72598424961487	19.614225040623918
42-43	20.753244302795988	29.79593094886925	29.154066669219823	20.296758079114937
44-45	20.739606004278095	29.992564708519758	28.493101805580206	20.77472748162194
46-47	20.025493547494577	27.80950719138114	28.78697157542314	23.378027685701138
48-49	21.10810129183519	28.284110333747442	30.0749694088196	20.532818965597766
50-51	22.169816079284868	30.830697665729474	27.818844167125924	19.180642087859738
52-53	20.405707168447332	30.130061461555375	28.182548983452886	21.281682386544404
54-55	22.484751027497854	28.442239261442243	27.777775701647062	21.295234009412845
56-57	21.30040293575486	28.730294618044333	29.215925061348397	20.753377384852413
58-59	21.188454462870045	27.840878728961037	28.829239546241155	22.141427261927763
60-61	19.64772705618963	30.477981960223715	29.413649357593112	20.46064162599354
62-63	20.371486208274643	31.15198129527668	27.089040065722124	21.387492430726553
64-65	20.066435711558192	30.20071199534304	28.59543450173403	21.13741779136474
66-67	20.27889815705652	30.28052345946552	29.338782144091464	20.101796239386495
68-69	21.013030441727302	29.773933512054324	29.138944674332368	20.074091371886006
70-71	19.607666943777613	30.668074016645335	29.385560962851564	20.338698076725485
72-73	20.23802273555209	30.57447901585137	28.43320847772682	20.754289770869725
74-75	20.092567510765296	29.131546746125895	30.170189712022566	20.605696031086243
76-77	19.795492121019645	30.248278954201968	28.500611823607986	21.455617101170404
78-79	19.43325518181903	29.522067683570434	28.242936006052847	22.801741128557683
80-81	21.1771615900488	29.3012198006218	28.079700465659172	21.44191814367022
82-83	20.35691267268838	27.930485629150827	29.772289528008443	21.94031217015235
84-85	20.63960320203256	29.0246317382329	28.460278169573215	21.875486890161316
86-87	21.443006996273013	27.95850808447836	29.17473869060407	21.423746228644553
88-89	21.17533650298439	28.122009770496092	28.910711116508033	21.79194261001149
90-91	20.745714899539497	28.360331412238338	28.466536517929704	22.42741717029246
92-93	20.646832995507065	29.190113677760444	28.52060117507496	21.64245215165753
94-95	21.309657472187713	28.622005100086866	28.239199678675845	21.829137749049572
96-97	20.01066721466135	29.967456588546288	28.64944842467097	21.372427772121394
98-99	22.277683850658995	29.393930336176055	27.649028087841053	20.679357725323893
100	20.72215735542748	31.088560299653455	27.537218491084186	20.652063853834875
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	8.5
2	11.5
3	13.0
4	20.0
5	25.5
6	35.0
7	39.0
8	44.5
9	56.0
10	58.0
11	66.5
12	90.0
13	103.0
14	120.0
15	163.0
16	214.0
17	265.0
18	375.5
19	642.0
20	1054.5
21	1657.0
22	2651.5
23	4126.5
24	6343.0
25	9455.0
26	13566.5
27	18290.0
28	23625.5
29	29996.0
30	37216.5
31	45310.0
32	54592.5
33	64879.0
34	76131.0
35	88734.5
36	101744.5
37	111640.5
38	118156.5
39	123917.5
40	128118.5
41	129149.0
42	127453.0
43	126421.0
44	126718.5
45	123507.0
46	115280.0
47	106227.5
48	98998.0
49	95049.5
50	92304.0
51	85945.5
52	75813.5
53	64440.5
54	53481.0
55	42704.0
56	32414.5
57	23791.5
58	17099.5
59	12357.5
60	9479.5
61	7863.5
62	6523.0
63	4979.5
64	3932.0
65	4012.5
66	4677.0
67	4299.0
68	2830.5
69	2674.0
70	4017.0
71	4055.0
72	2220.0
73	723.0
74	268.5
75	161.0
76	117.5
77	97.0
78	76.0
79	52.5
80	47.0
81	42.5
82	37.5
83	30.0
84	24.0
85	19.0
86	15.0
87	19.5
88	17.0
89	11.5
90	15.5
91	16.5
92	13.0
93	15.0
94	18.5
95	16.0
96	13.5
97	16.0
98	46.5
99	97.5
100	92.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.329740231839114
2	0.006463846362218257
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.9890619016038184E-4
30-31	0.009378181716281981
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0011022165762164082
40-41	0.0017187105934221955
42-43	9.340818442511933E-5
44-45	0.0
46-47	0.0022978413368579358
48-49	0.0
50-51	0.0
52-53	0.001363759492606742
54-55	0.0189431798014142
56-57	0.011806794511335083
58-59	0.014123317485078042
60-61	2.2417964262028638E-4
62-63	0.011563933231829773
64-65	1.1208982131014319E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4945309508019092E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2676425.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.87706398511659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.147146579129	63.642982599615316
2	8.729185664023742	12.897732156780892
3	2.3413901849765923	5.189250975289191
4	0.9744960797776344	2.8797163695591035
5	0.5280938687799261	1.9507012267001183
6	0.3099209851154176	1.3737631468621248
7	0.2018601592985555	1.0438985133181649
8	0.1337099818177199	0.7902480705757177
9	0.10871501100227536	0.7228391241561977
>10	0.4858492274567864	6.27353265703305
>50	0.027005524763573076	1.3495790423104723
>100	0.012008543677776288	1.5481020258313196
>500	5.666743326419202E-4	0.29837782699067517
>1k	5.151584841525674E-5	0.03927626497769378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.736327377004773E-5	0.0
16-17	0.0	0.0	0.0	3.736327377004773E-5	0.0
18-19	0.0	0.0	0.0	3.736327377004773E-5	0.0
20-21	0.0	0.0	0.0	1.1208982131014319E-4	0.0
22-23	0.0	0.0	0.0	1.1208982131014319E-4	0.0
24-25	0.0	0.0	0.0	1.1208982131014319E-4	0.0
26-27	0.0	0.0	0.0	1.1208982131014319E-4	0.0
28-29	0.0	0.0	0.0	1.1208982131014319E-4	0.0
30-31	0.0	0.0	0.0	1.1208982131014319E-4	0.0
32-33	0.0	0.0	0.0	1.4945309508019092E-4	0.0
34-35	0.0	0.0	0.0	1.4945309508019092E-4	0.0
36-37	0.0	0.0	0.0	1.4945309508019092E-4	0.0
38-39	0.0	0.0	0.0	1.4945309508019092E-4	0.0
40-41	0.0	0.0	0.0	1.4945309508019092E-4	0.0
42-43	0.0	0.0	0.0	1.4945309508019092E-4	0.0
44-45	0.0	0.0	0.0	1.4945309508019092E-4	0.0
46-47	0.0	0.0	0.0	1.4945309508019092E-4	0.0
48-49	0.0	0.0	0.0	1.4945309508019092E-4	0.0
50-51	0.0	0.0	0.0	1.4945309508019092E-4	0.0
52-53	0.0	0.0	0.0	1.4945309508019092E-4	0.0
54-55	0.0	3.736327377004773E-5	0.0	1.4945309508019092E-4	0.0
56-57	3.736327377004773E-5	3.736327377004773E-5	1.8681636885023865E-4	1.4945309508019092E-4	0.0
58-59	1.1208982131014319E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
60-61	1.8681636885023865E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
62-63	2.054980057352625E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
64-65	2.2417964262028638E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
66-67	2.6154291639033414E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
68-69	3.175878270454057E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
70-71	3.923143745855012E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
72-73	4.4835928524057276E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
74-75	5.604491065507159E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
76-77	6.538572909758353E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
78-79	8.033103860560262E-4	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
80-81	0.0011208982131014318	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
82-83	0.0015692574983420047	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
84-85	0.0017000289565371719	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
86-87	0.0017187105934221957	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
88	0.0017187105934221957	3.736327377004773E-5	3.736327377004773E-4	1.4945309508019092E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10225	0.0	80.37971	8
GGCGCGC	605	0.0	75.25579	6
CACCGGC	10765	0.0	73.4263	9
GGCTGCA	80	0.0	72.83275	1
CTTCACC	11765	0.0	72.73079	6
GTGTGGC	4245	0.0	70.655624	7
AGGCGCG	655	0.0	69.51108	5
CCTTCAC	13095	0.0	68.999954	5
CGTAGTG	2380	0.0	68.829094	4
GCGCGCA	665	0.0	68.4658	7
TTCACCG	12805	0.0	65.834015	7
AACGGGC	765	0.0	65.03813	5
CGGGGCA	1080	0.0	64.322296	94
CGCAGCA	30	1.3798557E-4	62.589706	2
CGGGCCC	820	0.0	62.39305	7
GGGCCCG	825	0.0	62.014915	8
TGCTGCG	60	9.95351E-9	61.797485	1
CAGCGTA	1075	0.0	61.592175	1
GCCTTCA	3895	0.0	60.49505	4
TGTGGCA	5125	0.0	60.0805	8
>>END_MODULE