FastQCFastQC Report
Mon 6 Mar 2017
1HL_14A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_14A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2015611
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA114730.5692070543373696No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG57410.2848267845333251No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA49130.24374742943950992No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT45660.22653180598835787No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA45610.22628374224986864No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG38270.1898679854396508No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG37830.18768502454094566No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37510.18609741661461462No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG34710.17220584725921817No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC32630.16188639573806654No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA31640.15697473371597992No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG31400.15578402777123165No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC29080.14427387030533173No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA28900.14338084084677052No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA28490.1413467181911589No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT27900.13841956607698608No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA26000.12899314401439563No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA25230.12517296244166162No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG23580.11698685907151726No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA23260.11539925114518626No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC23130.11475428542511427No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC22460.1114302313293587No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA22320.11073565286158887No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA21540.106865858541157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAT550.085.999483
TGCAGCA302.4596375E-885.999482
AGCAGCG950.085.999482
GCTGCTA202.8584467E-585.999473
GCTGCCC800.085.999473
GCAGCAA402.1827873E-1185.999473
CAGCAGC3250.084.6764141
CAGCTGC3250.084.6764141
AGCAGCA1250.082.5594942
AGCTGCC1100.082.090412
CAGCCCT117300.080.244191
CAGCGTA42500.079.62541
AGCTGCA650.079.384132
AGCAGCC650.079.384132
CTGCAGC600.078.8328551
AGCCCTT98100.078.635612
AGCAGCT501.8189894E-1277.399532
GCCCTTC90200.077.037233
CAGCTCT46650.076.781961
GCTGCAA456.184564E-1176.4439853
Produced by FastQC (version 0.11.5)