##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_14A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2015611 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.791518303879071 12.0 12.0 12.0 2.0 27.0 2 14.624879006911552 12.0 12.0 12.0 12.0 22.0 3 13.740214257612207 12.0 12.0 12.0 12.0 22.0 4 13.71447268346918 12.0 12.0 12.0 12.0 22.0 5 22.426664668926694 22.0 22.0 22.0 22.0 27.0 6 26.748396888090014 27.0 22.0 32.0 22.0 32.0 7 34.919397641707654 37.0 37.0 37.0 27.0 41.0 8 37.53384011101348 41.0 37.0 41.0 27.0 41.0 9 38.50799732686515 41.0 37.0 41.0 32.0 41.0 10-11 38.872250399506655 41.0 41.0 41.0 37.0 41.0 12-13 39.05265624170537 41.0 41.0 41.0 37.0 41.0 14-15 39.005489650532766 41.0 41.0 41.0 37.0 41.0 16-17 38.899260323544574 41.0 41.0 41.0 37.0 41.0 18-19 38.841809257837944 41.0 41.0 41.0 37.0 41.0 20-21 38.85580476589977 41.0 41.0 41.0 37.0 41.0 22-23 38.83790498265786 41.0 41.0 41.0 34.5 41.0 24-25 38.8520294838637 41.0 41.0 41.0 37.0 41.0 26-27 38.83227914513267 41.0 41.0 41.0 37.0 41.0 28-29 38.775176857042354 41.0 41.0 41.0 34.5 41.0 30-31 38.63922056388857 41.0 41.0 41.0 32.0 41.0 32-33 38.68885613345036 41.0 41.0 41.0 32.0 41.0 34-35 38.66610546380229 41.0 41.0 41.0 32.0 41.0 36-37 38.58276993923927 41.0 41.0 41.0 32.0 41.0 38-39 38.488209282445865 41.0 41.0 41.0 32.0 41.0 40-41 38.53786792193533 41.0 41.0 41.0 32.0 41.0 42-43 38.27790233333714 41.0 41.0 41.0 32.0 41.0 44-45 38.396570816491874 41.0 41.0 41.0 32.0 41.0 46-47 38.38927625419786 41.0 41.0 41.0 32.0 41.0 48-49 38.36362398300069 41.0 41.0 41.0 32.0 41.0 50-51 38.24727043065353 41.0 41.0 41.0 32.0 41.0 52-53 38.20268667912608 41.0 41.0 41.0 32.0 41.0 54-55 38.06159993173286 41.0 37.0 41.0 32.0 41.0 56-57 37.86063903203545 41.0 37.0 41.0 27.0 41.0 58-59 37.86506845814991 41.0 37.0 41.0 29.5 41.0 60-61 37.85364214622762 41.0 37.0 41.0 29.5 41.0 62-63 37.84306793324704 41.0 37.0 41.0 29.5 41.0 64-65 37.824631340075044 41.0 37.0 41.0 27.0 41.0 66-67 37.69740168117757 41.0 37.0 41.0 27.0 41.0 68-69 37.72735984274743 41.0 37.0 41.0 27.0 41.0 70-71 37.70683232032371 41.0 37.0 41.0 27.0 41.0 72-73 37.55606240489857 41.0 37.0 41.0 27.0 41.0 74-75 37.503308178016496 41.0 37.0 41.0 27.0 41.0 76-77 36.4132255678303 39.0 34.5 41.0 24.5 41.0 78-79 36.94413480577353 41.0 37.0 41.0 27.0 41.0 80-81 37.5984837848176 41.0 37.0 41.0 27.0 41.0 82-83 37.76747547021722 41.0 37.0 41.0 27.0 41.0 84-85 37.68631472044953 41.0 37.0 41.0 27.0 41.0 86-87 37.53495664590042 41.0 37.0 41.0 27.0 41.0 88-89 37.44031040711725 41.0 37.0 41.0 27.0 41.0 90-91 37.29268618795988 41.0 37.0 41.0 27.0 41.0 92-93 37.18052962600422 41.0 37.0 41.0 27.0 41.0 94-95 37.022790359846226 41.0 37.0 41.0 27.0 41.0 96-97 36.97462804082733 41.0 37.0 41.0 27.0 41.0 98-99 36.853049025828895 41.0 37.0 41.0 27.0 41.0 100 36.72506401284772 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3289101577371643 1101 2 -0.5503858590942805 1101 3 -0.016721614420633912 1101 4 -0.004875446007213569 1101 5 -1.11073905212006 1101 6 -0.5531833637946484 1101 7 -0.28862664929851434 1101 8 0.09946843252451032 1101 9 0.10827656904199046 1101 10-11 -0.05582740638796224 1101 12-13 -0.02575153006178965 1101 14-15 0.026623259296812307 1101 16-17 0.021357705037395647 1101 18-19 -0.0012356419636745386 1101 20-21 0.05264916273585385 1101 22-23 0.2964513190515561 1101 24-25 0.10088848105975501 1101 26-27 0.1609900378542406 1101 28-29 0.08393334010371234 1101 30-31 -0.10484173057961499 1101 32-33 -0.052571104957984005 1101 34-35 0.10448334978769935 1101 36-37 0.08793202614361206 1101 38-39 0.12429436830107221 1101 40-41 0.012426904497274904 1101 42-43 0.2783344556033853 1101 44-45 0.12745110438906693 1101 46-47 0.15737767115891899 1101 48-49 0.20663351531683105 1101 50-51 0.09883696863286673 1101 52-53 0.012122058678457392 1101 54-55 -0.06348099041429123 1101 56-57 0.10411741765101823 1101 58-59 0.1759068776420989 1101 60-61 0.10345042620050293 1101 62-63 -0.07718379882836501 1101 64-65 -0.11249498735823238 1101 66-67 -0.02552911574894523 1101 68-69 -0.003585598822986924 1101 70-71 -0.06324851965359812 1101 72-73 0.18640879626942564 1101 74-75 0.23323077939451053 1101 76-77 -0.2541483466034222 1101 78-79 -0.09799504582684193 1101 80-81 -0.11513896477510599 1101 82-83 -0.1576066563550782 1101 84-85 -0.22972672697844132 1101 86-87 -0.13256986251067104 1101 88-89 -0.36348668049573263 1101 90-91 -0.14564350666740467 1101 92-93 -0.019117917096814097 1101 94-95 -0.02128369097113847 1101 96-97 -0.18643607982905053 1101 98-99 -0.368407422648211 1101 100 -0.9071265571533615 1102 1 1.8232172226867291 1102 2 0.9760653370070784 1102 3 -0.1191553393856779 1102 4 0.06744670302648892 1102 5 -0.8454131788016923 1102 6 -0.04596307143480516 1102 7 -0.11198446994657729 1102 8 -0.4324534624159426 1102 9 -0.16545994338193992 1102 10-11 -0.30798300225761466 1102 12-13 -0.3101496578091414 1102 14-15 -0.2039444254596816 1102 16-17 -0.28609003765948415 1102 18-19 -0.0482098275903553 1102 20-21 -0.10964240735820852 1102 22-23 -0.1943137966670605 1102 24-25 -0.06198745263436933 1102 26-27 -0.13549993353801426 1102 28-29 -0.19444199555562847 1102 30-31 -0.22662256434374228 1102 32-33 -0.24120545549093464 1102 34-35 -0.06420728786807217 1102 36-37 -0.22190992226070705 1102 38-39 -0.13873411036329486 1102 40-41 -0.10175179757966646 1102 42-43 0.010825644048885863 1102 44-45 -0.23023076526924058 1102 46-47 -0.3773167715368899 1102 48-49 -0.280159644767636 1102 50-51 -0.11274128363034919 1102 52-53 -0.11679942485476147 1102 54-55 -0.20356197468861126 1102 56-57 -0.13148428291700043 1102 58-59 -0.06340718504567633 1102 60-61 -0.2575689608090741 1102 62-63 -0.379463198389999 1102 64-65 -0.3916346375412658 1102 66-67 -0.14371283864741713 1102 68-69 -0.29570047364263985 1102 70-71 -0.4784875014747314 1102 72-73 -0.44646397715582964 1102 74-75 -0.5109726389099976 1102 76-77 -0.41967887435560414 1102 78-79 -0.20528458431949304 1102 80-81 -0.2597147479590447 1102 82-83 -0.22277273579673817 1102 84-85 -0.5417018568705814 1102 86-87 -0.31627940773081065 1102 88-89 -0.6132433016405443 1102 90-91 -0.41770720675089024 1102 92-93 -0.4952429442007258 1102 94-95 -0.14669931083847843 1102 96-97 -0.37176009640996455 1102 98-99 -0.3469762532406335 1102 100 -0.6155873027351859 1103 1 1.6427997544935362 1103 2 0.9025992099463949 1103 3 0.4960868815513546 1103 4 1.116313419871915 1103 5 2.6991143981562367 1103 6 -0.1656195702888681 1103 7 -0.4805072302640667 1103 8 -0.1797135265207217 1103 9 -0.215241652768853 1103 10-11 -0.31436111503752784 1103 12-13 -0.3532811636728397 1103 14-15 -0.369462770523441 1103 16-17 -0.4241172968258411 1103 18-19 -0.33776869904968976 1103 20-21 -0.2132424622642688 1103 22-23 -0.3018218422911332 1103 24-25 -0.3620796349615176 1103 26-27 -0.18238655187780495 1103 28-29 -0.26458724940182066 1103 30-31 -0.3275376544670934 1103 32-33 -0.4423613451089423 1103 34-35 -0.4311267782826107 1103 36-37 -0.5240791344043103 1103 38-39 -0.455335841725649 1103 40-41 -0.6499385315214425 1103 42-43 -0.2701795271389642 1103 44-45 -0.7279410070055192 1103 46-47 -0.5160685759880508 1103 48-49 -0.6041901202319835 1103 50-51 -0.454196625944256 1103 52-53 -0.3671229087091916 1103 54-55 -0.3599918709070522 1103 56-57 -0.39369177317019677 1103 58-59 -0.5674711440977305 1103 60-61 -0.3284966585082927 1103 62-63 -0.43105212376003976 1103 64-65 -0.4588599169669152 1103 66-67 -0.6586410736973818 1103 68-69 -0.5388011111123845 1103 70-71 -0.5991064827071426 1103 72-73 -0.5809587731154195 1103 74-75 -0.5652836101682439 1103 76-77 -0.585916235065632 1103 78-79 -0.5687015366299377 1103 80-81 -0.2896354594478723 1103 82-83 -0.5037203336892304 1103 84-85 -0.6199429894446311 1103 86-87 -0.5246475723657156 1103 88-89 -0.35614113148510995 1103 90-91 -0.7093248927467926 1103 92-93 -0.5145986307183605 1103 94-95 -0.49647562069601037 1103 96-97 -0.2639939467753436 1103 98-99 -0.45712974299551945 1103 100 -0.30044767285666296 1104 1 1.6969800616844246 1104 2 -0.3534904967779049 1104 3 -0.07306650866058817 1104 4 0.06457785546914785 1104 5 -1.0870730628391918 1104 6 -0.341257972597262 1104 7 -0.26152554875915257 1104 8 -0.009593515682404075 1104 9 0.17027862670764193 1104 10-11 0.057889797794253184 1104 12-13 0.1286867640596867 1104 14-15 0.01825513554211966 1104 16-17 0.11994993295646594 1104 18-19 0.1646048407658256 1104 20-21 0.1661084501486627 1104 22-23 0.035919953821569095 1104 24-25 -0.006847038047347098 1104 26-27 -0.012665889838366695 1104 28-29 -0.026664771718792224 1104 30-31 -0.09490626645696665 1104 32-33 -0.013471206140714287 1104 34-35 -0.08570617652405588 1104 36-37 0.034401542365444016 1104 38-39 -0.12725525800303927 1104 40-41 0.1288678231344491 1104 42-43 -5.268597024610244E-5 1104 44-45 0.042025385835778195 1104 46-47 -0.04662173931703961 1104 48-49 -0.13271834834439744 1104 50-51 -0.06789488578871072 1104 52-53 0.040195307040974626 1104 54-55 -0.0322503873141784 1104 56-57 0.09030753348682907 1104 58-59 0.1690522435012909 1104 60-61 0.09280214814921806 1104 62-63 -0.13714236209024477 1104 64-65 -0.11472129090076066 1104 66-67 -0.31857358230054444 1104 68-69 -0.3209011670759807 1104 70-71 -0.04337759140830855 1104 72-73 -0.0941011987139504 1104 74-75 -0.07436472775206227 1104 76-77 -0.2606926303458792 1104 78-79 -0.1493459911910051 1104 80-81 -0.23746945535547326 1104 82-83 -0.09444822460240943 1104 84-85 -0.18616996970079214 1104 86-87 -0.04642424440320525 1104 88-89 0.006849206497044236 1104 90-91 -0.18279485846304055 1104 92-93 -0.08144307928677819 1104 94-95 0.12652670902659935 1104 96-97 0.057692062291593516 1104 98-99 -0.05554304435516855 1104 100 -0.025255271792545386 1105 1 1.5226692500333687 1105 2 0.37741060289619277 1105 3 -0.1312250679864011 1105 4 -0.13057991061411123 1105 5 -1.2156656571066193 1105 6 0.4451540902164943 1105 7 0.05547691136046495 1105 8 0.004897449328879588 1105 9 -0.05485215154306644 1105 10-11 -0.12704233066456538 1105 12-13 -0.20185467579801042 1105 14-15 -0.09071946499462769 1105 16-17 -0.03154526330983032 1105 18-19 -0.1672429684590213 1105 20-21 -0.10147132474128284 1105 22-23 -0.0728324697134255 1105 24-25 0.01443767573529442 1105 26-27 -0.1651438464254582 1105 28-29 0.06273579944096497 1105 30-31 -0.2624322792229634 1105 32-33 -0.17050512589572264 1105 34-35 -0.1780171878988881 1105 36-37 -0.14873731150897385 1105 38-39 0.1253840163590283 1105 40-41 0.0595219059469585 1105 42-43 0.01325956331517375 1105 44-45 0.03654098972700126 1105 46-47 -0.21621046534939836 1105 48-49 -0.18557983111131193 1105 50-51 -0.2502626363023026 1105 52-53 -0.21793056164755598 1105 54-55 -0.21028545077044214 1105 56-57 -0.08108341701622379 1105 58-59 -0.2373190706372057 1105 60-61 -0.28195000280678784 1105 62-63 -0.4769128618832994 1105 64-65 -0.451060058856271 1105 66-67 -0.17389223279586474 1105 68-69 -0.3247661048130439 1105 70-71 -0.37858584741148604 1105 72-73 -0.31673040523901363 1105 74-75 -0.29690663363191305 1105 76-77 -0.30772302667196527 1105 78-79 -0.36664420579860035 1105 80-81 -0.3386413252034046 1105 82-83 -0.36318771633679603 1105 84-85 -0.3355869081613747 1105 86-87 -0.2672738366966385 1105 88-89 -0.17470222017764314 1105 90-91 -0.3575135965102305 1105 92-93 -0.3015864156410899 1105 94-95 -0.27434136713724655 1105 96-97 -0.4585530804323952 1105 98-99 -0.3168919712166982 1105 100 -0.4200887693641491 1106 1 1.3369880110357002 1106 2 -0.6316136927956588 1106 3 -0.09573162331105323 1106 4 -0.287303964175214 1106 5 -1.2051256610468037 1106 6 -0.5850626835342432 1106 7 -0.2672083885702605 1106 8 -0.3000296890161138 1106 9 -0.3454942111161259 1106 10-11 -0.33211436615165724 1106 12-13 -0.16978156629263452 1106 14-15 -0.2592065608873 1106 16-17 -0.19263275449208095 1106 18-19 -0.21479687591551055 1106 20-21 -0.1850820598400702 1106 22-23 -0.14289786408149752 1106 24-25 -0.23536817480457728 1106 26-27 -0.25685198036634205 1106 28-29 -0.303912119569155 1106 30-31 -0.055622749058301224 1106 32-33 -0.14273380917472167 1106 34-35 -0.20525022444729046 1106 36-37 -0.4759217312531163 1106 38-39 -0.24020418342108485 1106 40-41 -0.4400256082408873 1106 42-43 -0.07297520818584502 1106 44-45 -0.22250205362139752 1106 46-47 -0.5148574565094037 1106 48-49 -0.3777093106890774 1106 50-51 -0.3774232242796529 1106 52-53 -0.2279230625849351 1106 54-55 -0.40387025690696987 1106 56-57 -0.3668587120534923 1106 58-59 -0.31356801409554436 1106 60-61 -0.46273873896053175 1106 62-63 -0.4911776042142364 1106 64-65 -0.672053621101675 1106 66-67 -0.48543093876270405 1106 68-69 -0.6131136418793801 1106 70-71 -0.4141514229009857 1106 72-73 -0.43102120363497676 1106 74-75 -0.5466276430820045 1106 76-77 -0.41095429649205073 1106 78-79 -0.3841561057941547 1106 80-81 -0.4047252257327827 1106 82-83 -0.2382202864180769 1106 84-85 -0.42134726862479255 1106 86-87 -0.5479923485775657 1106 88-89 -0.46817326934733927 1106 90-91 -0.5668734434785137 1106 92-93 -0.5053114796691105 1106 94-95 -0.5786498328163319 1106 96-97 -0.3535013813863088 1106 98-99 -0.623524193036431 1106 100 -0.6419129481214654 1107 1 1.4122324116195148 1107 2 -0.4269814993064074 1107 3 -0.0015324044986755325 1107 4 -0.12539436604290977 1107 5 -1.2197002714909573 1107 6 -0.3017617666128771 1107 7 -0.28209917879615176 1107 8 -0.2931162104955831 1107 9 -0.37593242351867673 1107 10-11 -0.2930822914693536 1107 12-13 -0.40416356256874053 1107 14-15 -0.3774986790682817 1107 16-17 -0.2634646905662734 1107 18-19 -0.3634013733333745 1107 20-21 -0.3070902485770475 1107 22-23 -0.2736621568448143 1107 24-25 -0.4000908148738205 1107 26-27 -0.5013090932936208 1107 28-29 -0.5879991744023911 1107 30-31 -0.6330176512182248 1107 32-33 -0.3476251742729417 1107 34-35 -0.392956579660904 1107 36-37 -0.24579410959576364 1107 38-39 -0.3175088625624838 1107 40-41 -0.4060512922591144 1107 42-43 -0.26138147046176385 1107 44-45 -0.2048775857775169 1107 46-47 -0.4979689849352482 1107 48-49 -0.43678973852183844 1107 50-51 -0.5295636655899827 1107 52-53 -0.2957768406139536 1107 54-55 -0.6154794842466202 1107 56-57 -0.2979008597765187 1107 58-59 -0.6278395390721485 1107 60-61 -0.5894305403229794 1107 62-63 -0.5981139179412338 1107 64-65 -0.5009772046002823 1107 66-67 -0.48418841609516505 1107 68-69 -0.7922531218754187 1107 70-71 -0.679395599923339 1107 72-73 -0.4173983262712184 1107 74-75 -0.440953812867356 1107 76-77 -0.5763051442690497 1107 78-79 -0.8804560039139986 1107 80-81 -0.4758457157795064 1107 82-83 -0.5072852885099763 1107 84-85 -0.462470920469336 1107 86-87 -0.28090059575989557 1107 88-89 -0.34920008714156126 1107 90-91 -0.2623399364059864 1107 92-93 -0.3882059937902014 1107 94-95 -0.27314958210811824 1107 96-97 -0.5154020370601344 1107 98-99 -0.5818475459473547 1107 100 -0.7170545104328312 1108 1 1.714150420520859 1108 2 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2223 34-35 0.21149638526188141 2223 36-37 0.2295105595983884 2223 38-39 0.07206653655730832 2223 40-41 0.1970520055590299 2223 42-43 0.28505745644925895 2223 44-45 0.10334502040187488 2223 46-47 0.007861097948534734 2223 48-49 0.03507866072169463 2223 50-51 -0.00877179128911365 2223 52-53 0.025399407087341785 2223 54-55 0.10993058211137452 2223 56-57 0.28521228841604085 2223 58-59 0.08495956088366796 2223 60-61 0.2924831835392183 2223 62-63 0.33967197090613865 2223 64-65 0.43249453959458606 2223 66-67 0.39720914569151944 2223 68-69 0.3538823103327857 2223 70-71 0.3682512874903807 2223 72-73 0.2990060782708852 2223 74-75 0.31403102007391936 2223 76-77 0.34510280831158724 2223 78-79 0.18497529233447807 2223 80-81 0.203999613690641 2223 82-83 0.14434092908442864 2223 84-85 0.11047758092578164 2223 86-87 0.23856003482988086 2223 88-89 0.16604035810273388 2223 90-91 0.173700182375768 2223 92-93 0.24545879686323957 2223 94-95 0.36511660460868 2223 96-97 0.23479870116524637 2223 98-99 0.412301337794581 2223 100 0.6469002429408732 2224 1 1.1613373673383762 2224 2 -0.9592973086148682 2224 3 -0.15491472972950326 2224 4 -0.4130333334444547 2224 5 -1.078377490258621 2224 6 0.563812003878926 2224 7 -0.0401545773263976 2224 8 -0.0698639022234886 2224 9 -0.005760951877448406 2224 10-11 0.0411837722201156 2224 12-13 0.14923760569958233 2224 14-15 0.012938479401668701 2224 16-17 -0.03219466983893682 2224 18-19 -0.027703238794089202 2224 20-21 0.03811419837980168 2224 22-23 0.1261305697427062 2224 24-25 0.0904028727086228 2224 26-27 0.12657860220289763 2224 28-29 0.046634328518273094 2224 30-31 0.003226931408491396 2224 32-33 0.1450637271779769 2224 34-35 0.10193695717723017 2224 36-37 -0.0402636780902057 2224 38-39 -0.04314481507802981 2224 40-41 0.010775540226276803 2224 42-43 0.1657901896098437 2224 44-45 0.12549739066868426 2224 46-47 0.02145818672482136 2224 48-49 0.12600468393905828 2224 50-51 -0.08169292882721635 2224 52-53 0.018798248890476543 2224 54-55 0.1410496282463214 2224 56-57 0.33741099249407114 2224 58-59 0.3100820738918557 2224 60-61 0.11243492038928338 2224 62-63 0.10074607829015747 2224 64-65 -0.03301036834628235 2224 66-67 0.10862296509948521 2224 68-69 0.17342428532261778 2224 70-71 0.13859007936815715 2224 72-73 0.11546393112467257 2224 74-75 0.14068364709118697 2224 76-77 0.13622547566688326 2224 78-79 0.21462596329912031 2224 80-81 0.237203087687611 2224 82-83 0.23831486223432563 2224 84-85 0.002895576609994066 2224 86-87 0.25850019564767734 2224 88-89 0.25952423921985 2224 90-91 0.20898096052259518 2224 92-93 0.12688727924540188 2224 94-95 0.30014983375772175 2224 96-97 0.32526123785978456 2224 98-99 0.006224811316627665 2224 100 0.4348769009106732 2225 1 1.0624266565951714 2225 2 1.436192240340029 2225 3 0.8593596485279953 2225 4 0.8716246405496655 2225 5 3.0702094995879037 2225 6 -0.12337261622242934 2225 7 0.16425848693084788 2225 8 0.42767257273548154 2225 9 0.24172773141710735 2225 10-11 -0.013792872985391114 2225 12-13 0.10433508266039127 2225 14-15 0.007526298839351853 2225 16-17 0.20009626417250104 2225 18-19 0.14455685606785806 2225 20-21 0.1680308785724094 2225 22-23 0.08177629824998434 2225 24-25 0.15898210768129672 2225 26-27 0.040091019018348106 2225 28-29 0.1349767693197279 2225 30-31 0.17416599252771903 2225 32-33 0.11564660513790415 2225 34-35 0.11984440365958449 2225 36-37 0.08865659098588452 2225 38-39 -0.06220918053096369 2225 40-41 -0.025189039838281246 2225 42-43 0.22925391303136422 2225 44-45 0.041783527479083205 2225 46-47 0.10696108127657311 2225 48-49 0.09211318003457336 2225 50-51 0.014793031244202837 2225 52-53 -0.014721742452920239 2225 54-55 0.08631825346949995 2225 56-57 0.14890083000241106 2225 58-59 0.3120776013134545 2225 60-61 0.2254649119130292 2225 62-63 0.20501396409513717 2225 64-65 0.021598371216200007 2225 66-67 0.251639971718177 2225 68-69 0.3223052389917598 2225 70-71 0.27722017188560244 2225 72-73 0.2118579147853339 2225 74-75 0.2941444849782613 2225 76-77 0.5097119226722384 2225 78-79 0.3343900708574097 2225 80-81 0.08453937869375494 2225 82-83 0.2099906363691133 2225 84-85 0.002135418626984631 2225 86-87 0.14959020628808872 2225 88-89 0.08339392127002299 2225 90-91 0.29916112608013634 2225 92-93 0.3018056727240932 2225 94-95 0.4149490185942355 2225 96-97 0.2476033901077983 2225 98-99 0.381510208708832 2225 100 0.5084832779418207 2226 1 1.377175269559629 2226 2 1.270319995877644 2226 3 0.6618569531669447 2226 4 1.0511338560302814 2226 5 3.115907080993299 2226 6 -0.5230868804941124 2226 7 0.2293416175400793 2226 8 0.06514605386063721 2226 9 0.21739626332853845 2226 10-11 0.2027855652244881 2226 12-13 0.27559713399032404 2226 14-15 0.21862981351694089 2226 16-17 0.09214591006886508 2226 18-19 0.2345013746461717 2226 20-21 0.17180758735810286 2226 22-23 0.28229743189412915 2226 24-25 0.36530490302239116 2226 26-27 0.2105206659763823 2226 28-29 0.23083552324836631 2226 30-31 0.14048496219507456 2226 32-33 0.07159964636548466 2226 34-35 0.1820120596930792 2226 36-37 0.0644775091841936 2226 38-39 0.1346240219240471 2226 40-41 0.20400176609754794 2226 42-43 0.445933484482687 2226 44-45 0.1663700709920093 2226 46-47 0.18847127056505286 2226 48-49 0.253149333566725 2226 50-51 0.2041089678295407 2226 52-53 0.3189987344200489 2226 54-55 0.32248130405075415 2226 56-57 0.4553528562366509 2226 58-59 0.2916614998031335 2226 60-61 0.32255648463102915 2226 62-63 0.32641595454376215 2226 64-65 0.3263224376328253 2226 66-67 0.28105006746984884 2226 68-69 0.27436579446494136 2226 70-71 0.20545995925868965 2226 72-73 0.3633422395905299 2226 74-75 0.4661725862908881 2226 76-77 0.5791657482544608 2226 78-79 0.5301529083724432 2226 80-81 0.37969010840899386 2226 82-83 0.3412071274025763 2226 84-85 0.28590476893972294 2226 86-87 0.4359148870090763 2226 88-89 0.3766423392898659 2226 90-91 0.6556709035137871 2226 92-93 0.38555543705161455 2226 94-95 0.4994488163277495 2226 96-97 0.5609603913653274 2226 98-99 0.5919735179349317 2226 100 0.5003715682683563 2227 1 0.9919976259075387 2227 2 1.8160127043408973 2227 3 0.7533572381814633 2227 4 -0.06740415960963908 2227 5 3.0476544956144345 2227 6 0.3311288861210322 2227 7 0.09958563985713198 2227 8 0.14742825180145047 2227 9 0.19431763227282772 2227 10-11 0.26645312773905516 2227 12-13 0.0746794519113152 2227 14-15 0.024536620082471927 2227 16-17 0.1476518331663712 2227 18-19 0.21347743496639282 2227 20-21 0.233744737576302 2227 22-23 0.04643198091986278 2227 24-25 0.13788271845308486 2227 26-27 0.17445485795637694 2227 28-29 0.1086642269050202 2227 30-31 0.15495972709273786 2227 32-33 0.14240642992660213 2227 34-35 0.05495455820641837 2227 36-37 0.13514509602739366 2227 38-39 0.20416633264500206 2227 40-41 0.08156377869731557 2227 42-43 0.36456561188586534 2227 44-45 0.2736066681496254 2227 46-47 0.2779538190845088 2227 48-49 0.1136352656134818 2227 50-51 0.22459213441937464 2227 52-53 0.258491892069884 2227 54-55 0.2396269213712685 2227 56-57 0.3993380336339314 2227 58-59 0.44814001377347523 2227 60-61 0.1861517079663173 2227 62-63 0.12863389410590287 2227 64-65 0.29787441636652545 2227 66-67 0.3268185334388676 2227 68-69 0.20189465545700358 2227 70-71 0.3413874020456049 2227 72-73 0.26050296529816563 2227 74-75 0.32258547455194986 2227 76-77 0.5130740995340162 2227 78-79 0.3243892095830532 2227 80-81 0.1744955147848799 2227 82-83 0.056480240909799306 2227 84-85 0.20562660339232508 2227 86-87 0.0505392267870306 2227 88-89 0.2353967811895501 2227 90-91 0.20641195666014056 2227 92-93 0.22070310105660695 2227 94-95 0.3151157148862964 2227 96-97 0.1967318537592746 2227 98-99 0.31345212583224935 2227 100 0.35791389007577123 2228 1 1.537893099560538 2228 2 1.5685733714297267 2228 3 -0.16781748390096674 2228 4 -0.0582258695849589 2228 5 3.225632232496647 2228 6 0.08170223908918928 2228 7 0.22900308848130635 2228 8 0.5358016130642653 2228 9 0.3168718679043394 2228 10-11 0.31245321316168884 2228 12-13 0.30258300109772307 2228 14-15 0.2768637677032899 2228 16-17 0.08760173712781949 2228 18-19 0.0907508065665894 2228 20-21 0.16106044908799788 2228 22-23 0.23989411672079797 2228 24-25 0.35407686799344873 2228 26-27 0.3508242662561116 2228 28-29 0.21375789157911385 2228 30-31 0.16142182513439707 2228 32-33 0.06845225359572282 2228 34-35 0.14996773371834138 2228 36-37 0.21705580214445774 2228 38-39 0.17621022997195723 2228 40-41 0.2364439796509572 2228 42-43 0.2845923900228726 2228 44-45 0.2561688456095652 2228 46-47 0.3226470311424734 2228 48-49 0.18127544074224744 2228 50-51 0.22306427642892146 2228 52-53 0.24158641344627796 2228 54-55 0.10620853681221121 2228 56-57 0.4123081908053834 2228 58-59 0.3780216539126968 2228 60-61 0.3509887519224506 2228 62-63 0.44755602766603886 2228 64-65 0.2623274608475725 2228 66-67 0.4572933965924335 2228 68-69 0.5399219003234421 2228 70-71 0.5834554776904355 2228 72-73 0.6370541133349619 2228 74-75 0.3469129725293598 2228 76-77 0.4678838268191754 2228 78-79 0.5252131665449085 2228 80-81 0.4316691585613768 2228 82-83 0.37489215996628644 2228 84-85 0.3974630784832698 2228 86-87 0.41541486567710706 2228 88-89 0.4849340752854303 2228 90-91 0.5988040502387761 2228 92-93 0.6725979358969099 2228 94-95 0.7564568315828879 2228 96-97 0.726016030702958 2228 98-99 0.6571760696093065 2228 100 0.6614372020967778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 16.0 13 22.0 14 107.0 15 336.0 16 1361.0 17 3385.0 18 5664.0 19 7222.0 20 8531.0 21 9859.0 22 11272.0 23 12568.0 24 14326.0 25 16124.0 26 18552.0 27 20672.0 28 23371.0 29 26417.0 30 30469.0 31 34739.0 32 40865.0 33 48779.0 34 60174.0 35 76860.0 36 108391.0 37 179112.0 38 439552.0 39 815945.0 40 919.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.876658101801198 19.45882960021984 18.931673417258192 35.73283888072077 2 18.637241836460287 53.37233798696691 10.329865848733258 17.660554327839538 3 42.74217594565618 14.261630840474677 23.598551506218215 19.39764170765093 4 17.173948743085845 16.531910175128036 21.743183580561922 44.55095750122419 5 17.1979613129716 35.595707703520176 26.25422266498843 20.952108318519794 6 17.50754485860615 27.74721908145967 30.798998417849475 23.946237642084707 7 20.241802609729753 26.526001296877226 36.6307784587403 16.60141763465272 8 20.62674791911733 27.583745077795268 34.62419087810098 17.16531612498642 9 20.781738142925395 27.95390578836889 27.797079892895997 23.46727617580972 10-11 19.50795565215709 30.13391472858602 28.67815267926202 21.67997693999487 12-13 24.264652256809473 26.500252280822046 27.329578971339213 21.90551649102927 14-15 21.68761730314034 27.29430430772604 28.7914185822562 22.226659806877418 16-17 20.872678309455544 29.727635937688373 28.137448148477063 21.26223760437902 18-19 21.51794170601371 29.42512220860077 29.867246209710107 19.189689875675413 20-21 22.92156075750728 28.9294412463516 29.89887433636748 18.25012365977364 22-23 22.029970068629314 30.419808187194853 28.54789441018133 19.002327333994508 24-25 22.205797646470472 28.22972785919505 26.632966380913775 22.931508113420694 26-27 20.220617966462775 26.31896730073412 30.056146746569652 23.404267986233453 28-29 19.82671220796321 27.794115567696377 30.794602021773592 21.584570202566823 30-31 20.01750008310927 30.123215073391684 28.167467906177322 21.69181693732172 32-33 20.61342689636046 30.0849221402344 28.942613430865382 20.359037532539762 34-35 21.301481287808013 29.225232448126153 29.983712134930794 19.489574129135036 36-37 22.00007838814136 28.015103112654177 28.64560671677223 21.339211782432223 38-39 21.62074467451746 29.287450039492118 28.91745948584833 20.174345800142092 40-41 20.813581992879968 29.80193547218779 29.933609697057502 19.450872837874748 42-43 20.588199543710996 29.922328666504434 29.309064731568657 20.18040705821592 44-45 20.544539596182002 30.17162041683638 28.75522608281062 20.528613904170992 46-47 19.757621793151216 27.89395947248643 29.008830756972348 23.339587977390007 48-49 20.948006049782954 28.090609813061505 30.152665980191102 20.80871815696443 50-51 21.96425798430352 30.614959930264323 28.025472176923028 19.39530990850913 52-53 20.33486597546863 30.47813721343582 28.221419862496326 20.965576948599228 54-55 22.615386485769644 28.048351132496187 28.203719054503573 21.1325433272306 56-57 21.56411026126392 28.55107807650609 29.390193981824904 20.49461768040508 58-59 21.30970453917872 27.93784279784321 28.82033752135838 21.932115141619686 60-61 19.490926915473743 30.58325678475117 29.532926722479537 20.392889577295545 62-63 20.39028030557138 31.217065633540606 27.38256262367444 21.01009143721358 64-65 19.849529385528296 30.242713184275026 28.888259357777386 21.019498072419285 66-67 20.27139661373152 30.116996781621058 29.402771665762888 20.208834938884536 68-69 20.85017892837457 29.992940106002596 29.275539774291765 19.881341191331064 70-71 19.735827002333288 30.872301252573042 29.221337847431872 20.1705338976618 72-73 20.104102428494386 30.685980578593785 28.620601891932523 20.589315100979306 74-75 19.9576207909165 29.12980232792935 30.57842014158486 20.33415673956929 76-77 19.514182051993167 30.337178155904095 28.646921454586227 21.501718337516515 78-79 19.463180147359783 29.447274300447855 28.512173231838883 22.57737232035348 80-81 21.111694337391 29.3107836444388 28.0748042278062 21.502717790364 82-83 20.391732333272643 27.776292151610605 30.12820926260077 21.703766252515987 84-85 21.34871262361636 28.538269537127945 28.423242381590498 21.689775457665196 86-87 22.169729178894144 28.468315562873986 29.366479940821915 19.995475317409955 88-89 22.044432184583236 27.912057435685757 29.410808930889935 20.632701448841072 90-91 22.456242796849192 27.73600660047995 29.105913789912837 20.701836812758017 92-93 22.116569119735903 28.051791739576732 30.189307361390664 19.642331779296697 94-95 21.894328816423407 27.84567557926604 30.038583833884612 20.22141177042594 96-97 21.872251143697866 28.034724954368674 30.067284808427818 20.025739093505642 98-99 22.893008621207166 28.87933237117678 28.924355939712576 19.303303067903478 100 22.422977449517788 30.253506256911677 27.77971543120176 19.54380086236878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 3.0 2 7.0 3 9.0 4 13.5 5 20.0 6 20.5 7 30.0 8 49.5 9 52.0 10 50.0 11 70.0 12 76.0 13 72.0 14 87.0 15 115.0 16 169.0 17 225.5 18 346.5 19 561.0 20 867.0 21 1371.0 22 2088.5 23 3214.0 24 4893.5 25 7302.0 26 10597.5 27 14209.0 28 18272.5 29 23078.0 30 28793.5 31 35045.5 32 41826.0 33 49730.0 34 58876.5 35 68994.5 36 78659.5 37 85534.0 38 90648.5 39 94870.5 40 97220.0 41 98093.0 42 97470.5 43 95915.5 44 94401.5 45 91503.5 46 85213.0 47 78077.5 48 72926.0 49 70627.5 50 68820.5 51 63646.5 52 55543.5 53 46783.5 54 38741.0 55 31692.5 56 25144.0 57 18656.5 58 12869.5 59 9030.0 60 6913.0 61 5758.5 62 4855.5 63 3700.5 64 2693.0 65 2427.0 66 2937.5 67 2820.0 68 1808.0 69 1569.5 70 2129.5 71 2077.5 72 1213.5 73 493.0 74 199.0 75 112.5 76 77.5 77 52.0 78 51.0 79 50.0 80 31.5 81 17.5 82 17.5 83 15.5 84 15.5 85 18.0 86 15.5 87 10.5 88 8.0 89 7.5 90 8.0 91 9.0 92 11.5 93 11.5 94 10.0 95 8.5 96 9.0 97 17.5 98 36.5 99 69.0 100 73.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.3540757616425 2 0.006201593462230559 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.2325736464030014E-4 30-31 0.00952564755798614 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.426422062590449E-4 40-41 0.0015131888047842563 42-43 1.240318692446112E-4 44-45 0.0 46-47 0.002083735403309468 48-49 7.441912154676672E-5 50-51 0.0 52-53 0.0014139633093885676 54-55 0.019572228966799648 56-57 0.010716353502734406 58-59 0.014958243430900109 60-61 3.2248286003598906E-4 62-63 0.01185744669978483 64-65 7.441912154676672E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4883824309353344E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2015611.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.8510129021191 #Duplication Level Percentage of deduplicated Percentage of total 1 87.02551763208157 66.00973660724613 2 8.102451231262906 12.29158265762627 3 2.244138935665293 5.106606340898879 4 0.9313142775031782 2.8256452511528516 5 0.5013801612341664 1.9015096539319654 6 0.3097487891032321 1.4096855639211017 7 0.19204884537978606 1.019696961411746 8 0.1393035823922355 0.8453054260275893 9 0.09683017879140789 0.6610200426739421 >10 0.42790946941097097 5.516416110592253 >50 0.020568582346679194 1.0652756156972059 >100 0.00832265101999155 1.1311529762221404 >500 4.6566380858265337E-4 0.2163667925980447 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 4.9612747697844474E-5 0.0 0.0 4 0.0 0.0 4.9612747697844474E-5 0.0 0.0 5 0.0 0.0 4.9612747697844474E-5 0.0 0.0 6 0.0 0.0 4.9612747697844474E-5 0.0 0.0 7 0.0 0.0 4.9612747697844474E-5 0.0 0.0 8 0.0 0.0 4.9612747697844474E-5 0.0 0.0 9 0.0 0.0 4.9612747697844474E-5 0.0 0.0 10-11 0.0 0.0 4.9612747697844474E-5 0.0 0.0 12-13 4.9612747697844474E-5 0.0 4.9612747697844474E-5 0.0 0.0 14-15 4.9612747697844474E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 16-17 4.9612747697844474E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 18-19 4.9612747697844474E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 20-21 4.9612747697844474E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 22-23 7.44191215467667E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 24-25 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 26-27 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 28-29 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 30-31 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 32-33 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 34-35 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 36-37 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 38-39 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 40-41 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 42-43 9.922549539568895E-5 0.0 4.9612747697844474E-5 4.9612747697844474E-5 0.0 44-45 9.922549539568895E-5 0.0 4.9612747697844474E-5 9.922549539568895E-5 0.0 46-47 9.922549539568895E-5 0.0 4.9612747697844474E-5 9.922549539568895E-5 0.0 48-49 9.922549539568895E-5 0.0 4.9612747697844474E-5 9.922549539568895E-5 0.0 50-51 9.922549539568895E-5 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 52-53 9.922549539568895E-5 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 54-55 9.922549539568895E-5 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 56-57 1.240318692446112E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 58-59 1.4883824309353344E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 60-61 1.7364461694245565E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 62-63 2.2325736464030014E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 64-65 2.480637384892224E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 66-67 2.9767648618706687E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 68-69 2.9767648618706687E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 70-71 2.9767648618706687E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 72-73 3.7209560773383355E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 74-75 4.465147292806003E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 76-77 6.449657200719781E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 78-79 8.186103370144338E-4 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 80-81 0.001041867701654734 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 82-83 0.0014387696832374897 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 84-85 0.0015876079263310232 0.0 4.9612747697844474E-5 1.4883824309353344E-4 0.0 86-87 0.0016868334217267121 9.922549539568895E-5 4.9612747697844474E-5 1.4883824309353344E-4 0.0 88 0.0016868334217267121 9.922549539568895E-5 4.9612747697844474E-5 1.4883824309353344E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 7845 0.0 78.73748 8 CACCGGC 7760 0.0 75.06348 9 CTTCACC 8780 0.0 73.560135 6 GGCGCGC 375 0.0 70.09312 6 CCTTCAC 9860 0.0 69.50155 5 GTGTGGC 3420 0.0 69.17083 7 CGTAGTG 1650 0.0 68.27251 4 AGGCGCG 395 0.0 67.73238 5 TTCACCG 9665 0.0 66.14451 7 CGGGGCA 860 0.0 66.039764 94 AGCTGCG 50 6.1227183E-9 65.715546 2 ACCTTCA 3960 0.0 61.990498 4 CAGCGTA 855 0.0 61.91499 1 GCAGCCC 85 0.0 60.742455 4 TGTGGCA 3875 0.0 60.56432 8 CGGGCCC 405 0.0 59.106297 7 ATGCAGC 40 8.097522E-6 58.674603 2 GCCTTCA 2640 0.0 57.960518 4 GCGTAGT 1215 0.0 57.561028 3 GCGCGCA 435 0.0 57.188038 7 >>END_MODULE