FastQCFastQC Report
Mon 6 Mar 2017
1HL_14A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_14A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2015611
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG78450.078.737488
CACCGGC77600.075.063489
CTTCACC87800.073.5601356
GGCGCGC3750.070.093126
CCTTCAC98600.069.501555
GTGTGGC34200.069.170837
CGTAGTG16500.068.272514
AGGCGCG3950.067.732385
TTCACCG96650.066.144517
CGGGGCA8600.066.03976494
AGCTGCG506.1227183E-965.7155462
ACCTTCA39600.061.9904984
CAGCGTA8550.061.914991
GCAGCCC850.060.7424554
TGTGGCA38750.060.564328
CGGGCCC4050.059.1062977
ATGCAGC408.097522E-658.6746032
GCCTTCA26400.057.9605184
GCGTAGT12150.057.5610283
GCGCGCA4350.057.1880387
Produced by FastQC (version 0.11.5)