FastQCFastQC Report
Mon 6 Mar 2017
1HL_15A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_15A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2196324
Sequences flagged as poor quality0
Sequence length92
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA113490.5167270402727466No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG66730.3038258471883019No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA59680.27172675798288415No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA47940.21827380659684092No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT46040.20962298822942335No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG42310.1926400658554931No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG41850.1905456571981183No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT38960.17738730715504636No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA37280.16973816249332976No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG36910.16805352944283267No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC34800.15844656799270052No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT33060.1505242395930655No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA28470.12962568364230415No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA27890.12698490750909244No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA26000.11837961976466133No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA25830.11760559917389236No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC24930.11350784310511565No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC23000.10472043286873886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCA1150.085.999852
GCTGCTT258.35882E-785.999843
GCTGCTA500.085.999843
GCTGCCC950.085.999843
GCTGCAT302.4596375E-885.999843
GCTGCAG202.8584436E-585.999843
GCTGCAC258.35882E-785.999843
GCTGCAA450.085.999843
TGCTGCC159.829516E-485.999842
GCAGCTC258.35882E-785.999843
GCAGCTA550.085.999843
GCAGCCC302.4596375E-885.999843
GCAGCAT500.085.999843
GCAGCAA800.085.999843
AGCAGCG900.085.999842
CAGCAGC4750.085.0945741
CAGCTGC4600.085.065061
AGCTGCC1700.083.4704362
AGCTGCT1200.082.416512
AGCAGCT1050.081.904612
Produced by FastQC (version 0.11.5)