##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_15A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2196324 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.820032017134084 12.0 12.0 12.0 2.0 32.0 2 14.587559941065162 12.0 12.0 12.0 12.0 22.0 3 13.746256472178057 12.0 12.0 12.0 12.0 22.0 4 13.743984494091036 12.0 12.0 12.0 12.0 22.0 5 22.435864653848885 22.0 22.0 22.0 22.0 27.0 6 26.799662527022424 27.0 22.0 32.0 22.0 32.0 7 34.90334395107461 37.0 37.0 37.0 27.0 41.0 8 37.49556440670867 41.0 37.0 41.0 27.0 41.0 9 38.490781414763944 41.0 37.0 41.0 32.0 41.0 10-11 38.86562160227726 41.0 41.0 41.0 37.0 41.0 12-13 39.04256794534868 41.0 41.0 41.0 37.0 41.0 14-15 38.990063396839446 41.0 41.0 41.0 37.0 41.0 16-17 38.89394734110268 41.0 41.0 41.0 37.0 41.0 18-19 38.82749494154778 41.0 41.0 41.0 37.0 41.0 20-21 38.850020079004736 41.0 41.0 41.0 37.0 41.0 22-23 38.829788091374496 41.0 41.0 41.0 34.5 41.0 24-25 38.84443233329873 41.0 41.0 41.0 37.0 41.0 26-27 38.80714252541975 41.0 41.0 41.0 37.0 41.0 28-29 38.758172063866716 41.0 41.0 41.0 34.5 41.0 30-31 38.64173318690685 41.0 41.0 41.0 32.0 41.0 32-33 38.68350707819065 41.0 41.0 41.0 32.0 41.0 34-35 38.66195447484069 41.0 41.0 41.0 32.0 41.0 36-37 38.58005922623438 41.0 41.0 41.0 32.0 41.0 38-39 38.48199218330265 41.0 41.0 41.0 32.0 41.0 40-41 38.53128796115691 41.0 41.0 41.0 32.0 41.0 42-43 38.274093212112604 41.0 41.0 41.0 32.0 41.0 44-45 38.384834614565065 41.0 41.0 41.0 32.0 41.0 46-47 38.38827217660054 41.0 41.0 41.0 32.0 41.0 48-49 38.36232177037632 41.0 41.0 41.0 32.0 41.0 50-51 38.22609209752295 41.0 41.0 41.0 32.0 41.0 52-53 38.20125150023403 41.0 41.0 41.0 32.0 41.0 54-55 38.05745714202458 41.0 37.0 41.0 32.0 41.0 56-57 37.84842741781267 41.0 37.0 41.0 27.0 41.0 58-59 37.847975754032646 41.0 37.0 41.0 29.5 41.0 60-61 37.847967786173626 41.0 37.0 41.0 29.5 41.0 62-63 37.844593511704105 41.0 37.0 41.0 29.5 41.0 64-65 37.83774001467907 41.0 37.0 41.0 27.0 41.0 66-67 37.69060530231423 41.0 37.0 41.0 27.0 41.0 68-69 37.72838797918705 41.0 37.0 41.0 27.0 41.0 70-71 37.698127871844044 41.0 37.0 41.0 27.0 41.0 72-73 37.548006578264406 41.0 37.0 41.0 27.0 41.0 74-75 37.47106210194853 41.0 37.0 41.0 27.0 41.0 76-77 36.400745746073895 39.0 34.5 41.0 24.5 41.0 78-79 36.930930044929625 41.0 37.0 41.0 27.0 41.0 80-81 37.57819565783555 41.0 37.0 41.0 27.0 41.0 82-83 37.77190364445319 41.0 37.0 41.0 27.0 41.0 84-85 37.69706404883797 41.0 37.0 41.0 27.0 41.0 86-87 37.54407273243838 41.0 37.0 41.0 27.0 41.0 88-89 37.44903643542574 41.0 37.0 41.0 27.0 41.0 90-91 37.28510024021956 41.0 37.0 41.0 27.0 41.0 92-93 37.17065173444355 41.0 37.0 41.0 27.0 41.0 94-95 37.01425609336327 41.0 37.0 41.0 27.0 41.0 96-97 36.95519172034727 41.0 37.0 41.0 27.0 41.0 98-99 36.82715869789703 41.0 37.0 41.0 27.0 41.0 100 36.6979762548695 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4990797314165967 1101 2 -0.551173083648866 1101 3 0.12132985611951597 1101 4 0.09834352229582244 1101 5 -1.1696471145049188 1101 6 -0.8478688353594919 1101 7 -0.39813134153200735 1101 8 -0.3295032387473711 1101 9 -0.29638990795167075 1101 10-11 -0.19601101160034062 1101 12-13 -0.25102320353818186 1101 14-15 -0.10631223469655993 1101 16-17 -0.2174221988062186 1101 18-19 -0.23447353959040385 1101 20-21 -0.03711403352258458 1101 22-23 -0.10595372806118775 1101 24-25 -0.24273961359445906 1101 26-27 -0.1862208253076929 1101 28-29 -0.23775299396459104 1101 30-31 -0.3256615678596262 1101 32-33 -0.3565952217878703 1101 34-35 -0.17693593265079244 1101 36-37 -0.20200753773040248 1101 38-39 -0.16631531006908773 1101 40-41 -0.1516149009725538 1101 42-43 -0.13569152709437304 1101 44-45 -0.1537786980276934 1101 46-47 -0.1772429820026602 1101 48-49 -0.22884817274142932 1101 50-51 -0.04337206216737144 1101 52-53 -0.09101752658582996 1101 54-55 -0.16913399096279846 1101 56-57 -0.0776829707459683 1101 58-59 -0.18804226416730074 1101 60-61 -0.3511315714318428 1101 62-63 -0.3415545719973423 1101 64-65 -0.2315357398255813 1101 66-67 -0.08034813554844789 1101 68-69 -0.18136715712024198 1101 70-71 -0.11359943225355806 1101 72-73 -0.04654734344037337 1101 74-75 -0.2157553660278282 1101 76-77 -0.48969383207612793 1101 78-79 -0.452049786166306 1101 80-81 -0.34127199594620095 1101 82-83 -0.24303188158152267 1101 84-85 -0.20993955241815598 1101 86-87 -0.23527114001413452 1101 88-89 -0.5337301560839052 1101 90-91 -0.32392315330050536 1101 92-93 -0.24303507184538375 1101 94-95 -0.2076977606397179 1101 96-97 -0.3624330230035895 1101 98-99 -0.25413746290308836 1101 100 -0.560326649080821 1102 1 1.4568180807042417 1102 2 0.6326232973306141 1102 3 0.19672905264547325 1102 4 0.1114917720637898 1102 5 -0.7120015779861397 1102 6 -0.21434459225778468 1102 7 -0.14044407314051455 1102 8 -0.31615581413234395 1102 9 -0.4098846135590506 1102 10-11 -0.3530705404908545 1102 12-13 -0.4841700983752162 1102 14-15 -0.36220435509572724 1102 16-17 -0.27188757693357957 1102 18-19 -0.23139933679789948 1102 20-21 -0.1196863698977424 1102 22-23 -0.18793138525010988 1102 24-25 -0.24368616834382806 1102 26-27 -0.18433516792907767 1102 28-29 -0.2655928953256179 1102 30-31 -0.41322967007561573 1102 32-33 -0.2564155579462266 1102 34-35 -0.16938287016814968 1102 36-37 -0.35106511552037034 1102 38-39 -0.36786386063172927 1102 40-41 -0.25649061993277655 1102 42-43 -0.2364737287216414 1102 44-45 -0.20457018093185297 1102 46-47 -0.32464591813799615 1102 48-49 -0.4358890713316299 1102 50-51 -0.2664229601478496 1102 52-53 -0.22080066989009595 1102 54-55 -0.14056391732230367 1102 56-57 -0.24064471427108458 1102 58-59 -0.22868287551214195 1102 60-61 -0.4123034511952781 1102 62-63 -0.43734886638318926 1102 64-65 -0.4680244363142805 1102 66-67 -0.40440940552521454 1102 68-69 -0.5606887432023839 1102 70-71 -0.5720338095611766 1102 72-73 -0.44386105737380177 1102 74-75 -0.5216930798528594 1102 76-77 -0.49547342546135553 1102 78-79 -0.5607216151198244 1102 80-81 -0.5585559013991173 1102 82-83 -0.44408168206793874 1102 84-85 -0.40906711239078675 1102 86-87 -0.3414104675619143 1102 88-89 -0.4457261725869728 1102 90-91 -0.518204125327955 1102 92-93 -0.4476146029037906 1102 94-95 -0.41021373188808496 1102 96-97 -0.39339780157401094 1102 98-99 -0.6543943578078739 1102 100 -0.7219444366141019 1103 1 1.5770863596151514 1103 2 0.43384487532843785 1103 3 0.47794126567104733 1103 4 1.2287370930206691 1103 5 2.7788551792544958 1103 6 0.04233800404304944 1103 7 -0.6710295216262807 1103 8 -0.4381662533219952 1103 9 -0.44022629732597807 1103 10-11 -0.3745948274446178 1103 12-13 -0.4589009242831352 1103 14-15 -0.5747731118956594 1103 16-17 -0.4993865784622642 1103 18-19 -0.5161512338933747 1103 20-21 -0.4395527813230018 1103 22-23 -0.2591869801324336 1103 24-25 -0.17715864496582157 1103 26-27 -0.25892937541830463 1103 28-29 -0.33088355376579415 1103 30-31 -0.22043711798296073 1103 32-33 -0.505967003319526 1103 34-35 -0.3822135707507073 1103 36-37 -0.33091720404691216 1103 38-39 -0.2921829032010379 1103 40-41 -0.26816722540577587 1103 42-43 -0.2547810099875676 1103 44-45 -0.5955216896564295 1103 46-47 -0.7120872149112145 1103 48-49 -0.49815718497831796 1103 50-51 -0.44749703983274003 1103 52-53 -0.33718606604219303 1103 54-55 -0.4879913043573225 1103 56-57 -0.4599574905722008 1103 58-59 -0.8273431335997614 1103 60-61 -0.4134041108749358 1103 62-63 -0.37383025350084154 1103 64-65 -0.5531845360236929 1103 66-67 -0.599346260822621 1103 68-69 -0.5632590149243057 1103 70-71 -0.6727808038182417 1103 72-73 -0.5455733323734648 1103 74-75 -0.6964133013069116 1103 76-77 -0.7656152772468943 1103 78-79 -0.8413332304398224 1103 80-81 -0.4439418622039568 1103 82-83 -0.5590017355033652 1103 84-85 -0.521548967757056 1103 86-87 -0.6104126848568185 1103 88-89 -0.38474670666582256 1103 90-91 -0.666167519304075 1103 92-93 -0.34916023648548133 1103 94-95 -0.5035195670921198 1103 96-97 -0.3717899184708955 1103 98-99 -0.500470296715207 1103 100 -0.48115502454155035 1104 1 1.8651197686105085 1104 2 -0.5668442486509804 1104 3 -0.04739300635691812 1104 4 0.20803082094415082 1104 5 -1.1390905140240868 1104 6 -0.38359523755421776 1104 7 -0.06226766058325239 1104 8 0.09983267188736278 1104 9 0.0028175230140519147 1104 10-11 -0.07860062954851799 1104 12-13 -0.03195054893402727 1104 14-15 -0.23635006370552958 1104 16-17 -0.12680885752602933 1104 18-19 -0.010677415638930654 1104 20-21 0.05079837948399302 1104 22-23 0.019889472352225823 1104 24-25 -0.007835255468982893 1104 26-27 -0.03535912835990729 1104 28-29 -0.078053400481231 1104 30-31 0.03354479375399677 1104 32-33 -0.08768723112217458 1104 34-35 -0.20553906568959235 1104 36-37 0.026746417379612808 1104 38-39 -0.11197118930721217 1104 40-41 -0.02898409939815849 1104 42-43 -0.055893705207175515 1104 44-45 0.0019921121340757963 1104 46-47 -0.28811403171016536 1104 48-49 -0.19641247092890524 1104 50-51 -0.19262186010325166 1104 52-53 -0.15710511466488697 1104 54-55 -0.12198343432974923 1104 56-57 -0.13406998826868488 1104 58-59 0.06129658311456154 1104 60-61 -0.06593251723849392 1104 62-63 -0.30068048793541635 1104 64-65 -0.16648004937967187 1104 66-67 -0.6248388823959061 1104 68-69 -0.38512432842882305 1104 70-71 -0.20558895368863972 1104 72-73 -0.13312021652890849 1104 74-75 -0.30067020054693927 1104 76-77 -0.4038216017387697 1104 78-79 -0.32031632841901825 1104 80-81 -0.2848761576257175 1104 82-83 -0.30287217357435026 1104 84-85 -0.019194414848996644 1104 86-87 -0.09268470840952148 1104 88-89 -0.23062542525937602 1104 90-91 -0.31673366030910444 1104 92-93 -0.2914597587884842 1104 94-95 -0.3754732694369025 1104 96-97 -0.42324441697733306 1104 98-99 -0.25715987532885265 1104 100 -0.2698461083034971 1105 1 1.5747121072075405 1105 2 0.30307866977274145 1105 3 -0.12086328878792685 1105 4 -0.038928668422876456 1105 5 -1.1674494413810237 1105 6 0.33301321549737395 1105 7 8.885015695412335E-4 1105 8 -0.0521271275870987 1105 9 -0.2181904798781673 1105 10-11 -0.24119830676431775 1105 12-13 -0.138832522044261 1105 14-15 -0.23584912092591992 1105 16-17 -0.1734785687232403 1105 18-19 -0.055078439152488556 1105 20-21 -0.09600223945864172 1105 22-23 -0.05266294508101055 1105 24-25 -0.12925371338156566 1105 26-27 -0.08637524705525834 1105 28-29 -0.10455274342454146 1105 30-31 -0.14851914880880912 1105 32-33 -0.2820976903833241 1105 34-35 -0.19541379288781258 1105 36-37 -0.133938798129158 1105 38-39 -0.09513116286591128 1105 40-41 -0.019976141034192096 1105 42-43 0.03125549569632824 1105 44-45 -0.09656943995098288 1105 46-47 -0.128626705562489 1105 48-49 -0.10152211378586173 1105 50-51 -0.2501446689026636 1105 52-53 -0.38535637908156417 1105 54-55 -0.17385380624133262 1105 56-57 -0.02019840561285946 1105 58-59 -0.04341198745979824 1105 60-61 -0.16142002998072513 1105 62-63 -0.3387262968996936 1105 64-65 -0.3643728561064066 1105 66-67 -0.09955505457814695 1105 68-69 -0.11571041027703899 1105 70-71 -0.48741273527122075 1105 72-73 -0.4283691320805545 1105 74-75 -0.45829131605375295 1105 76-77 -0.5681313635497531 1105 78-79 -0.45089660569217216 1105 80-81 -0.5420800593603587 1105 82-83 -0.3358082336620427 1105 84-85 -0.4340700564665809 1105 86-87 -0.437935267265523 1105 88-89 -0.2483409088440851 1105 90-91 -0.4964002338551978 1105 92-93 -0.22854942966642966 1105 94-95 -0.2705982480341831 1105 96-97 -0.2651285873445275 1105 98-99 -0.5482503031869967 1105 100 -0.44744172078688393 1106 1 1.4870631877099125 1106 2 -0.633519011065216 1106 3 -0.07326363072749231 1106 4 -0.2388649135041625 1106 5 -1.1882774148253574 1106 6 -0.6432708772404077 1106 7 -0.2180881192352686 1106 8 -0.21461017667121496 1106 9 -0.2355806077057494 1106 10-11 -0.13307785711788966 1106 12-13 -0.19693944170793998 1106 14-15 -0.06000662325010353 1106 16-17 -0.11452705013643083 1106 18-19 -0.1686620037084552 1106 20-21 -0.0857856931097416 1106 22-23 0.005446152704898566 1106 24-25 -0.3382105875865591 1106 26-27 -0.17778129926253428 1106 28-29 0.04252124405612534 1106 30-31 0.06931011408388343 1106 32-33 -0.07468151733895212 1106 34-35 -0.11740280797681635 1106 36-37 -0.32024473871408077 1106 38-39 -0.010640520591479685 1106 40-41 -0.12224423251737448 1106 42-43 -0.014426902308620981 1106 44-45 -0.09177705856126295 1106 46-47 -0.3736901837315614 1106 48-49 -0.19406540204316514 1106 50-51 -0.07540686356837512 1106 52-53 -0.14755117290820152 1106 54-55 -0.34350428681197087 1106 56-57 -0.2846935527565506 1106 58-59 -0.1862537553365442 1106 60-61 -0.18857288598584176 1106 62-63 -0.42236349963726383 1106 64-65 -0.5484744088769347 1106 66-67 -0.4034161734052262 1106 68-69 -0.3401811522018434 1106 70-71 -0.5943915926231824 1106 72-73 -0.186880080604098 1106 74-75 -0.4766354614149648 1106 76-77 -0.27150506987328527 1106 78-79 -0.2280949460142878 1106 80-81 -0.32679625259721945 1106 82-83 -0.2956904254372432 1106 84-85 -0.5184945863253034 1106 86-87 -0.32409109679184667 1106 88-89 -0.22585512813846265 1106 90-91 -0.5195878824159408 1106 92-93 -0.2965692398161721 1106 94-95 -0.29637799016501987 1106 96-97 -0.3807372930684281 1106 98-99 -0.5918582319618864 1106 100 -0.3781782028478702 1107 1 1.2217584627377658 1107 2 -0.6355578562138966 1107 3 -0.11598412977088124 1107 4 -0.136016502670687 1107 5 -1.1811105652118137 1107 6 -0.04158913890867666 1107 7 -0.3194609416353771 1107 8 0.0036384141572796125 1107 9 0.06858831576341373 1107 10-11 0.10890422987696269 1107 12-13 -0.18765239908372422 1107 14-15 -0.27483937616472787 1107 16-17 -0.09112975747565599 1107 18-19 -0.05724349264205131 1107 20-21 -0.14322423428514242 1107 22-23 0.02206196837681773 1107 24-25 -0.02436821914870535 1107 26-27 -0.16397121253953628 1107 28-29 -0.06729476561500292 1107 30-31 -0.1707893877376918 1107 32-33 0.06752175346294109 1107 34-35 -0.15253298515040115 1107 36-37 -0.0989257809552413 1107 38-39 -0.19342607733007355 1107 40-41 -0.09165923841766954 1107 42-43 0.002409015964580874 1107 44-45 0.11914713552855005 1107 46-47 -0.2182341620333048 1107 48-49 -0.05989909406221727 1107 50-51 -0.00491250540299859 1107 52-53 -0.10910890208778312 1107 54-55 -0.14348765683764952 1107 56-57 0.03993152627444374 1107 58-59 -0.3792927467054312 1107 60-61 -0.05730910061239314 1107 62-63 -0.17372247230643723 1107 64-65 -0.18697377765870726 1107 66-67 -0.24963318890662833 1107 68-69 -0.3688786699593507 1107 70-71 -0.2705843685211491 1107 72-73 -0.04870121210137057 1107 74-75 -0.11771376336587025 1107 76-77 -0.2852191352275355 1107 78-79 -0.6145786383422518 1107 80-81 -0.29761450911689025 1107 82-83 -0.09876192505934256 1107 84-85 -0.24585053367778897 1107 86-87 0.045577529088312474 1107 88-89 0.10096685802622574 1107 90-91 -0.04405891536903539 1107 92-93 -0.12447552090083747 1107 94-95 0.03777946222589179 1107 96-97 0.027378494801062914 1107 98-99 -0.1358095035628324 1107 100 -0.09520554602669762 1108 1 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0.2671351113333458 2223 34-35 0.1988637170042722 2223 36-37 0.11131971192838819 2223 38-39 0.18187673125720494 2223 40-41 0.23472404775247924 2223 42-43 0.3623763725837037 2223 44-45 0.28897085603629336 2223 46-47 0.22285134163548292 2223 48-49 0.12946117944061797 2223 50-51 0.2507345115364785 2223 52-53 0.4013330531643433 2223 54-55 0.27472156445934814 2223 56-57 0.4614697441605031 2223 58-59 0.40947303507343946 2223 60-61 0.3492058323600489 2223 62-63 0.3949957710765375 2223 64-65 0.46461847065087625 2223 66-67 0.7041095435563918 2223 68-69 0.6984682634737993 2223 70-71 0.5665492442136895 2223 72-73 0.5428709318645772 2223 74-75 0.6089668995539199 2223 76-77 0.46127948914322303 2223 78-79 0.6747824204865154 2223 80-81 0.4963675383325068 2223 82-83 0.28530616793371877 2223 84-85 0.49842483300397333 2223 86-87 0.6527025294155351 2223 88-89 0.42960049562972813 2223 90-91 0.6018662901900313 2223 92-93 0.5253797173539638 2223 94-95 0.7272738955739655 2223 96-97 0.7171658547185515 2223 98-99 0.6126917019124534 2223 100 0.6471962947772667 2224 1 1.468161167996696 2224 2 -0.7827638471704539 2224 3 -0.0395359481764892 2224 4 -0.2796310291855928 2224 5 -1.0616645732187564 2224 6 0.5475097559256774 2224 7 0.3296397670699065 2224 8 0.33760055607972106 2224 9 0.15337693501895444 2224 10-11 0.3324892162081383 2224 12-13 0.09544193516087063 2224 14-15 0.12385515501441802 2224 16-17 0.1508018855027231 2224 18-19 0.11402233014275254 2224 20-21 0.12527828569982802 2224 22-23 0.2684256754107466 2224 24-25 0.24209058654685123 2224 26-27 0.14830662066207623 2224 28-29 0.14292263977219477 2224 30-31 0.12971953262042035 2224 32-33 0.04657309896647632 2224 34-35 0.2685260563483709 2224 36-37 0.19050647744361981 2224 38-39 0.11217230573355153 2224 40-41 0.11931079384331866 2224 42-43 0.14061652279898595 2224 44-45 0.25160933575399014 2224 46-47 0.2796217129650813 2224 48-49 0.04652421158798603 2224 50-51 0.15411953533135403 2224 52-53 0.32744526139380525 2224 54-55 0.360603269920837 2224 56-57 0.34512217706542003 2224 58-59 0.47755746642440755 2224 60-61 0.30546080986012925 2224 62-63 0.15702561989002817 2224 64-65 0.1128336928982776 2224 66-67 0.14272523198916076 2224 68-69 0.4427306222422942 2224 70-71 0.32942599645559767 2224 72-73 0.4528149248717952 2224 74-75 0.6488050296325909 2224 76-77 0.5874396108694029 2224 78-79 0.21692428328557156 2224 80-81 0.16361736836908847 2224 82-83 0.47638519282931924 2224 84-85 0.1297065190410791 2224 86-87 0.2876113966498224 2224 88-89 0.42200685388023373 2224 90-91 0.4698445348725997 2224 92-93 0.550616533048867 2224 94-95 0.6211688096210466 2224 96-97 0.500907639820042 2224 98-99 0.4017687196210318 2224 100 0.7069755042045713 2225 1 1.1952155572114744 2225 2 1.9243229594571059 2225 3 0.9344850974137131 2225 4 0.8967955942578563 2225 5 3.1992775900003423 2225 6 -0.10934987153135722 2225 7 0.29536761517117327 2225 8 0.44522893932558105 2225 9 0.351263628442851 2225 10-11 0.2711943206642573 2225 12-13 0.34795441329197985 2225 14-15 0.2363129609215946 2225 16-17 0.23350269336523155 2225 18-19 0.2489048813416872 2225 20-21 0.33451711970208464 2225 22-23 0.27132291735784975 2225 24-25 0.35546109699246387 2225 26-27 0.26700735134026843 2225 28-29 0.2649983833144418 2225 30-31 0.2079386331658597 2225 32-33 0.28013232506506824 2225 34-35 0.16694249146209472 2225 36-37 0.35860426997217587 2225 38-39 0.20852406039034577 2225 40-41 0.3540270128409517 2225 42-43 0.46750380796931523 2225 44-45 0.39230528100458173 2225 46-47 0.37577510392936375 2225 48-49 0.4351673113101242 2225 50-51 0.1793145543701513 2225 52-53 0.3625863800518374 2225 54-55 0.44512331211492295 2225 56-57 0.6568019650658385 2225 58-59 0.5259508748838044 2225 60-61 0.5841104702491364 2225 62-63 0.46642911376674334 2225 64-65 0.4561054074192228 2225 66-67 0.47315064621166414 2225 68-69 0.4585022081227095 2225 70-71 0.33604519476783423 2225 72-73 0.5965373957990394 2225 74-75 0.5493338708387157 2225 76-77 0.6510955746442306 2225 78-79 0.4511287702671396 2225 80-81 0.5074102056645415 2225 82-83 0.5450102828776906 2225 84-85 0.35187141046846904 2225 86-87 0.48028853465537225 2225 88-89 0.40366129969685005 2225 90-91 0.576608196496565 2225 92-93 0.4131453485268963 2225 94-95 0.6457381211369437 2225 96-97 0.49306957251531713 2225 98-99 0.39720776973938143 2225 100 0.7180761525528254 2226 1 1.2161174725189703 2226 2 1.3537676948553798 2226 3 0.6901306408323382 2226 4 0.9257403047172303 2226 5 3.088580048975853 2226 6 -0.2514454872727896 2226 7 -0.0577765566079691 2226 8 0.15394882560419632 2226 9 0.10739236304307553 2226 10-11 0.12989363235062257 2226 12-13 0.2317219952162617 2226 14-15 0.13112379936740126 2226 16-17 0.11434618930019269 2226 18-19 0.027879206744451324 2226 20-21 0.10580402927364219 2226 22-23 0.30799868672290387 2226 24-25 0.2304158952367672 2226 26-27 0.20501548142156878 2226 28-29 0.1607911909357682 2226 30-31 0.12039062578394066 2226 32-33 0.26715786249835816 2226 34-35 0.07495224257186806 2226 36-37 0.12247497424670684 2226 38-39 0.25209231815881594 2226 40-41 0.18014988679975374 2226 42-43 0.47887750491180014 2226 44-45 0.3318315666048619 2226 46-47 0.17038767812260147 2226 48-49 0.20180779213210798 2226 50-51 0.1904248341846113 2226 52-53 0.10194838065037004 2226 54-55 0.17128640004948892 2226 56-57 0.374396982273673 2226 58-59 0.38684948578708855 2226 60-61 0.36829079096337836 2226 62-63 0.3235619508159715 2226 64-65 0.3054728945756935 2226 66-67 0.4503331116712843 2226 68-69 0.5024539014739986 2226 70-71 0.4542072602075038 2226 72-73 0.3498402413274917 2226 74-75 0.3502419585238883 2226 76-77 0.5682111415054223 2226 78-79 0.33117889745553697 2226 80-81 0.3179119763264282 2226 82-83 0.23390997862311025 2226 84-85 0.2797714927668409 2226 86-87 0.41232535694077654 2226 88-89 0.3578469822745305 2226 90-91 0.35909423071272784 2226 92-93 0.30033735311565835 2226 94-95 0.5656032016895907 2226 96-97 0.3484769494418458 2226 98-99 0.5889551760938048 2226 100 0.7150149026151738 2227 1 1.3139424038836118 2227 2 1.5430777765924315 2227 3 0.7005510206876249 2227 4 0.12248770783036811 2227 5 3.3097453804580645 2227 6 0.10218452592270921 2227 7 0.34385130528330166 2227 8 0.5316856410485613 2227 9 0.19478753386538727 2227 10-11 0.3531555473866135 2227 12-13 0.3302809653419061 2227 14-15 0.30403722187979554 2227 16-17 0.33096188756726974 2227 18-19 0.2159471291724273 2227 20-21 0.3123808289584531 2227 22-23 0.29814192981402954 2227 24-25 0.3232010524883506 2227 26-27 0.18615717357243966 2227 28-29 0.2242671732500483 2227 30-31 0.24393661929250499 2227 32-33 0.29685555422396703 2227 34-35 0.3277648852364905 2227 36-37 0.2931432856605056 2227 38-39 0.2773873407662677 2227 40-41 0.5011718501150142 2227 42-43 0.49674615027804947 2227 44-45 0.2957745507890195 2227 46-47 0.3021925848472975 2227 48-49 0.3557964602621624 2227 50-51 0.3928037764183401 2227 52-53 0.25904205619116283 2227 54-55 0.3462127616562114 2227 56-57 0.483284088832022 2227 58-59 0.44043040672040235 2227 60-61 0.34426763317397757 2227 62-63 0.3320131681164611 2227 64-65 0.30787982252575574 2227 66-67 0.26618553470274975 2227 68-69 0.26175641612350375 2227 70-71 0.35722893584835447 2227 72-73 0.38437133205123786 2227 74-75 0.29986297876741475 2227 76-77 0.6883988490531223 2227 78-79 0.45938005690845074 2227 80-81 0.4273494165245495 2227 82-83 0.4419235868731093 2227 84-85 0.30178796525328266 2227 86-87 0.3345352279250591 2227 88-89 0.4849271496259888 2227 90-91 0.20556912486730994 2227 92-93 0.5147884578478923 2227 94-95 0.6525566984970297 2227 96-97 0.4197201406265023 2227 98-99 0.5561165176571308 2227 100 0.7489151421353881 2228 1 1.4740033283000322 2228 2 1.3782015913123544 2228 3 -0.1391850079518111 2228 4 -0.03486850729192703 2228 5 3.2762594260883446 2228 6 0.05569891719778752 2228 7 0.5858199719882009 2228 8 0.5852745345357135 2228 9 0.39020859277844977 2228 10-11 0.2885925607262223 2228 12-13 0.5122031482463782 2228 14-15 0.42529125117332 2228 16-17 0.4138900311653728 2228 18-19 0.35570632996311957 2228 20-21 0.28794312174593983 2228 22-23 0.4395866010717313 2228 24-25 0.5807500847087468 2228 26-27 0.4622379852228846 2228 28-29 0.2892907613117117 2228 30-31 0.4467762540559974 2228 32-33 0.36536168154007953 2228 34-35 0.5335443090894927 2228 36-37 0.4631972971809617 2228 38-39 0.3200674984298928 2228 40-41 0.6323687214354266 2228 42-43 0.7064067157198721 2228 44-45 0.5776378087469922 2228 46-47 0.5053446690750931 2228 48-49 0.4736981483417537 2228 50-51 0.4958520380420026 2228 52-53 0.43441184187588533 2228 54-55 0.3564522950512341 2228 56-57 0.6344764342205593 2228 58-59 0.4961279143062498 2228 60-61 0.7199322502548213 2228 62-63 0.5640064432843346 2228 64-65 0.5656334516669759 2228 66-67 0.4816257048281898 2228 68-69 0.5209347321565119 2228 70-71 0.6208642098041537 2228 72-73 0.68594008927478 2228 74-75 0.7006102225213979 2228 76-77 0.5182230351991208 2228 78-79 0.544730297387332 2228 80-81 0.5551291059402246 2228 82-83 0.6547653502712407 2228 84-85 0.4196884908503762 2228 86-87 0.6477864176613224 2228 88-89 0.5531191483364069 2228 90-91 0.6353084239575253 2228 92-93 0.7259415216609497 2228 94-95 0.6963049192529738 2228 96-97 0.6648095561974685 2228 98-99 0.840661493588577 2228 100 0.8822482688051636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 15.0 13 30.0 14 137.0 15 388.0 16 1459.0 17 3569.0 18 5911.0 19 7748.0 20 8987.0 21 10428.0 22 12159.0 23 13793.0 24 15735.0 25 17584.0 26 20102.0 27 22663.0 28 25423.0 29 28994.0 30 33408.0 31 38232.0 32 44817.0 33 53638.0 34 65956.0 35 84975.0 36 120349.0 37 198141.0 38 483587.0 39 877085.0 40 1011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.1032358374985 19.255076503130677 21.787140655054525 34.8545470043163 2 20.260561164529552 52.43369974177926 10.726883870788544 16.578855222902643 3 40.565326427248436 14.539384899495703 24.55439179283202 20.340896880423838 4 17.99916587898689 17.092104807851666 21.215540147992737 43.69318916516871 5 17.1526150058006 34.61470165603982 26.795226933731087 21.437456404428488 6 17.191725810945925 27.8524024688525 30.88314838794276 24.07272333225881 7 19.937404499518287 27.10815890551667 36.547795316173755 16.40664127879129 8 20.446892170736195 27.671053997497637 34.95950506391589 16.92254876785028 9 20.437922638007873 27.961038535298073 28.446531568202143 23.154507258491915 10-11 19.309081902305852 30.24198615504816 28.85216388838805 21.596768054257932 12-13 24.266023592147608 26.507382335211016 27.470150123570107 21.756443949071265 14-15 21.77925479118746 27.294515745400037 28.614107026103618 22.312122437308883 16-17 20.902039043419823 29.94150680864936 28.154247733940895 21.00220641398992 18-19 21.457922419460882 29.533279242953224 29.891810133659696 19.116988203926198 20-21 22.79690974555667 28.982222112948726 29.917375578466565 18.303492563028044 22-23 22.053622325303554 30.653104915303935 28.58544549893367 18.70782726045884 24-25 22.244782645911986 28.35669964904996 26.556805826462764 22.841711878575293 26-27 20.061247794041318 26.431755970430594 30.12813683227065 23.378859403257444 28-29 19.447899087541682 28.201953313258844 30.775190039518385 21.574957559681092 30-31 20.036614791537936 30.211984206598313 28.158964932036557 21.592436069827198 32-33 20.394850668662727 30.150628960025934 29.016506672057492 20.438013699253844 34-35 21.40399139653348 29.229089150781036 29.95803442479343 19.408885027892058 36-37 21.75261937674041 28.304225606058118 28.79479530342518 21.14835971377629 38-39 21.5043332937973 29.275987412515136 29.00644422220285 20.21323507148472 40-41 20.787947126278205 30.05412082514623 29.815991926383113 19.34194012219245 42-43 20.429586861110145 30.106730828552035 29.391374979824203 20.072307330513617 44-45 20.429203523705976 30.16909162764692 28.93252543795906 20.46917941068804 46-47 19.705688642998307 27.96688672821469 29.219300184698717 23.108124444088286 48-49 20.760142856882684 28.190763293575994 30.429299138014244 20.619794711527078 50-51 21.88472647933547 30.772190259724887 28.13460127012226 19.208481990817383 52-53 20.23704042996104 30.449024993033714 28.36191635720569 20.952018219799555 54-55 22.607647249688338 28.29036095883783 28.30987470897302 20.79211708250081 56-57 21.593329494632535 28.73007990918262 29.3631467875015 20.31344380868334 58-59 21.196817836146796 27.918273762630978 29.141685526801126 21.743222874421104 60-61 19.526895418795604 30.546978084269977 29.57879127013252 20.3473352268019 62-63 20.30989661005758 31.378084792958283 27.49418844245704 20.817830154527094 64-65 19.713976431056913 30.339442408844786 28.83051100191343 21.116070158184876 66-67 20.259032820294273 30.418371788497506 29.454260846760317 19.868334544447904 68-69 20.782953698998874 30.106600847598074 29.388719514971378 19.72172593843167 70-71 19.639315510826272 30.990190882583807 29.245389113810166 20.125104492779755 72-73 19.921127301800645 30.9168638142642 28.593845898874665 20.568162985060493 74-75 19.926272262198108 29.243704480759668 30.59097838023898 20.23904487680324 76-77 19.33920040941136 30.679125666340667 28.705191037387927 21.276482886860045 78-79 19.48036810598072 29.5377184786944 28.68135575625454 22.30055765907034 80-81 20.91975150268301 29.410607691087122 28.319465069207766 21.350175737022106 82-83 20.188278232173396 28.14454971124479 30.107238276319887 21.55993378026193 84-85 21.269493936231633 28.52910135298799 28.252024746804207 21.94937996397617 86-87 21.638428574290497 28.571968434529698 28.99836271879741 20.7912402723824 88-89 21.75596587752991 27.982984295577516 29.05001721057549 21.211032616317084 90-91 21.64719321921538 28.25776160530049 28.72449602153416 21.370549153949963 92-93 21.031163890209275 28.838322579000184 29.962496425846098 20.168017104944443 94-95 21.643323116261534 28.541485682440292 29.025339612916856 20.789851588381314 96-97 20.65198486197847 29.087989750146154 29.57437063019846 20.685654757676918 98-99 22.2973249848383 29.443128609440137 28.22798457786738 20.031561827854176 100 21.320624825845368 30.85009315565463 27.666819649559898 20.1624623689401 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 6.0 2 11.5 3 15.5 4 16.5 5 22.0 6 25.5 7 28.0 8 39.5 9 53.0 10 62.0 11 71.5 12 88.5 13 111.5 14 128.5 15 148.5 16 199.5 17 267.0 18 379.5 19 636.0 20 1051.5 21 1602.0 22 2365.0 23 3604.5 24 5612.0 25 8457.0 26 12098.5 27 16307.0 28 20950.5 29 26215.5 30 32311.0 31 39309.0 32 47496.0 33 56381.5 34 65481.0 35 75826.5 36 86802.5 37 95059.0 38 100497.5 39 104424.5 40 106600.0 41 106858.5 42 105334.0 43 103645.0 44 102695.0 45 100113.5 46 92819.5 47 84379.5 48 78889.0 49 76367.5 50 74022.5 51 67925.0 52 58459.5 53 48802.0 54 40751.5 55 33623.5 56 26445.0 57 19393.5 58 13670.5 59 9716.0 60 7419.0 61 6169.5 62 5132.5 63 4011.5 64 2961.0 65 2426.5 66 2319.0 67 1979.0 68 1482.5 69 1493.5 70 1776.0 71 1676.5 72 1087.0 73 532.0 74 259.5 75 143.5 76 96.0 77 65.5 78 61.5 79 53.0 80 40.0 81 33.0 82 27.5 83 27.0 84 23.5 85 19.5 86 19.0 87 14.0 88 8.5 89 9.0 90 9.0 91 7.0 92 4.5 93 6.5 94 10.0 95 12.0 96 15.0 97 17.0 98 30.0 99 73.0 100 76.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.329020672724061 2 0.006419817841083556 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.048878034388369E-4 30-31 0.00988014518805058 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.05724656289327E-4 40-41 0.0015708064930310829 42-43 2.048878034388369E-4 44-45 0.0 46-47 0.002026112722895165 48-49 0.0 50-51 0.0 52-53 0.0014569799355650622 54-55 0.01987411693356718 56-57 0.0122249722718506 58-59 0.015207228077460338 60-61 9.106124597281639E-5 62-63 0.012247737583343805 64-65 6.82959344796123E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.138265574660205E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2196324.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.70968365237528 #Duplication Level Percentage of deduplicated Percentage of total 1 87.06512093704534 66.78737884236544 2 8.297258087653645 12.729600861720469 3 2.1541931479597873 4.957424247183292 4 0.8906464643724277 2.7328483411246185 5 0.48353419171553386 1.854587744080279 6 0.2824801761786432 1.3001378971638575 7 0.18669305560335825 1.0024815664800244 8 0.12889726165106252 0.791013453192836 9 0.093574294704046 0.6460249090267355 >10 0.3929060071551518 5.188099351620804 >50 0.017513289352914813 0.9226394303850454 >100 0.006821433081264309 0.9143275707261922 >500 3.6165352682892096E-4 0.1734357849302736 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.55306229864082E-5 0.0 0.0 0.0 0.0 12-13 4.55306229864082E-5 0.0 0.0 0.0 0.0 14-15 4.55306229864082E-5 0.0 0.0 0.0 0.0 16-17 4.55306229864082E-5 0.0 0.0 0.0 0.0 18-19 4.55306229864082E-5 0.0 0.0 0.0 0.0 20-21 4.55306229864082E-5 0.0 0.0 0.0 0.0 22-23 4.55306229864082E-5 0.0 0.0 0.0 0.0 24-25 4.55306229864082E-5 0.0 0.0 0.0 0.0 26-27 4.55306229864082E-5 0.0 0.0 0.0 0.0 28-29 4.55306229864082E-5 0.0 0.0 0.0 0.0 30-31 4.55306229864082E-5 0.0 0.0 0.0 0.0 32-33 4.55306229864082E-5 0.0 0.0 0.0 0.0 34-35 4.55306229864082E-5 0.0 0.0 0.0 0.0 36-37 4.55306229864082E-5 0.0 0.0 0.0 0.0 38-39 4.55306229864082E-5 0.0 0.0 0.0 0.0 40-41 4.55306229864082E-5 0.0 0.0 0.0 0.0 42-43 4.55306229864082E-5 0.0 0.0 0.0 0.0 44-45 4.55306229864082E-5 0.0 0.0 0.0 0.0 46-47 4.55306229864082E-5 0.0 0.0 0.0 0.0 48-49 4.55306229864082E-5 0.0 0.0 0.0 0.0 50-51 4.55306229864082E-5 0.0 0.0 0.0 0.0 52-53 4.55306229864082E-5 0.0 0.0 0.0 0.0 54-55 4.55306229864082E-5 0.0 0.0 0.0 0.0 56-57 1.365918689592246E-4 0.0 0.0 0.0 0.0 58-59 3.187143609048574E-4 0.0 0.0 0.0 0.0 60-61 4.3254091837087786E-4 0.0 0.0 0.0 0.0 62-63 5.008368528504902E-4 0.0 0.0 0.0 0.0 64-65 6.374287218097148E-4 0.0 0.0 0.0 0.0 66-67 6.374287218097148E-4 0.0 0.0 0.0 0.0 68-69 6.82959344796123E-4 0.0 0.0 0.0 0.0 70-71 7.057246562893272E-4 0.0 0.0 0.0 0.0 72-73 7.740205907689393E-4 0.0 0.0 0.0 0.0 74-75 8.423165252485518E-4 0.0 0.0 0.0 0.0 76-77 8.878471482349599E-4 0.0 0.0 0.0 0.0 78-79 9.561430827145722E-4 0.0 0.0 0.0 0.0 80-81 0.001183796197646613 0.0 0.0 0.0 0.0 82-83 0.0014569799355650622 0.0 0.0 0.0 0.0 84-85 0.001616337116017491 0.0 0.0 0.0 0.0 86-87 0.0016391024275106952 0.0 0.0 0.0 0.0 88 0.0016391024275106952 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 9135 0.0 80.92697 8 CACCGGC 8910 0.0 78.861565 9 CTTCACC 10070 0.0 75.92992 6 GTGTGGC 3645 0.0 73.40044 7 CCTTCAC 11025 0.0 72.88639 5 GCTGCTC 65 0.0 72.21341 2 TTCACCG 11260 0.0 67.613556 7 CGTAGTG 1925 0.0 67.05378 4 CAGCGTA 880 0.0 66.20161 1 CGGGGCA 845 0.0 66.10153 94 GCCTTCA 3105 0.0 63.944035 4 CGCAGCC 50 1.9998151E-7 63.553547 1 TGTGGCA 4130 0.0 62.73506 8 CGCTGCC 60 9.964424E-9 61.788174 1 ACCTTCA 4555 0.0 61.312622 4 CGGGCCC 600 0.0 61.018936 7 AACGGGC 570 0.0 60.113125 5 GCGTAGT 1290 0.0 59.672665 3 TGTGTGG 4230 0.0 59.14365 6 GGGCCCG 630 0.0 58.858322 8 >>END_MODULE