FastQCFastQC Report
Tue 7 Mar 2017
1HL_15A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_15A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2196324
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG91350.080.926978
CACCGGC89100.078.8615659
CTTCACC100700.075.929926
GTGTGGC36450.073.400447
CCTTCAC110250.072.886395
GCTGCTC650.072.213412
TTCACCG112600.067.6135567
CGTAGTG19250.067.053784
CAGCGTA8800.066.201611
CGGGGCA8450.066.1015394
GCCTTCA31050.063.9440354
CGCAGCC501.9998151E-763.5535471
TGTGGCA41300.062.735068
CGCTGCC609.964424E-961.7881741
ACCTTCA45550.061.3126224
CGGGCCC6000.061.0189367
AACGGGC5700.060.1131255
GCGTAGT12900.059.6726653
TGTGTGG42300.059.143656
GGGCCCG6300.058.8583228
Produced by FastQC (version 0.11.5)