FastQCFastQC Report
Mon 6 Mar 2017
1HL_16A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_16A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2333158
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA130570.5596277663150117No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG63830.2735777002671915No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA61380.26307691120789933No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA52190.22368823714467687No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT51380.22021654770058435No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG45570.19531467650283435No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG43570.18674260380137137No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC41760.17898487800654736No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG41080.17607037328804992No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT38820.16638393113539673No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA37330.1599977369728068No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG36150.15494021407894365No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG35320.15138280390783648No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT34230.14671102428553917No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33440.14332505556846128No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC33410.14319647447793935No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA33200.14229640684428574No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA29840.1278953247058279No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA29010.12433791453472076No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA28250.12108052690816482No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA27600.11829460328018934No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA27210.11662304910340406No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC25800.11057973784887264No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC24890.10667944476970698No Hit
CAGCCTTATTCAGGACACATCAGTACAAGTGACAGCCCTTCAACCGCTGA23640.1013218993312926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGCC800.085.9998552
CAGCAGC4750.085.999851
GCTGCTA500.085.999853
GCTGCCT302.4596375E-885.999853
GCTGCCA550.085.999853
TGCTGCC750.085.999852
GCAGCTC159.829587E-485.999853
GCAGCTA159.829587E-485.999853
GCAGCGG159.829587E-485.999853
GCAGCCT258.358984E-785.999853
GCAGCCA258.358984E-785.999853
GCAGCAA1200.085.999853
CAGCTGC5800.085.258481
AGCTGCC2550.084.3135762
AGCAGCA2350.084.170062
CTGCTGC2100.083.952241
AGCAGCG1150.082.260732
AGCTGCA1750.081.085572
GCTGCCC2550.080.941033
CAGCCCT138100.080.270681
Produced by FastQC (version 0.11.5)