FastQCFastQC Report
Tue 7 Mar 2017
1HL_16A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_16A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2333158
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG93450.079.862488
CACCGGC91900.077.327749
CTTCACC105550.073.731196
GTGTGGC36500.072.271737
CCTTCAC114700.071.573365
CGTAGTG19950.071.524644
GGCGCGC4250.068.474336
TTCACCG115650.066.967387
ATGCTGC506.1190804E-965.720312
GCCTTCA32950.064.2464
GGGCCCG6200.063.5934758
TGTGGCA41050.062.202918
ACCTTCA44850.061.3281974
CGGGGCA10800.060.8456194
GCGTAGT13900.059.7700273
CGGGCCC6700.059.5482677
AACGGGC6500.059.214155
CAGCGTA9700.058.9573061
GCAGCCT457.966824E-658.8359951
TGTGTGG42350.058.741836
Produced by FastQC (version 0.11.5)