##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_16A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2333158 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.829348891073815 12.0 12.0 12.0 2.0 32.0 2 14.618622485061021 12.0 12.0 12.0 12.0 22.0 3 13.73572257001026 12.0 12.0 12.0 12.0 22.0 4 13.738986386691343 12.0 12.0 12.0 12.0 22.0 5 22.42708637820499 22.0 22.0 22.0 22.0 27.0 6 26.789031861537023 27.0 22.0 32.0 22.0 32.0 7 34.92238931096822 37.0 37.0 37.0 27.0 41.0 8 37.568944323530594 41.0 37.0 41.0 27.0 41.0 9 38.522887862716544 41.0 37.0 41.0 32.0 41.0 10-11 38.8925372820872 41.0 41.0 41.0 37.0 41.0 12-13 39.06540062867582 41.0 41.0 41.0 37.0 41.0 14-15 39.01247922343879 41.0 41.0 41.0 37.0 41.0 16-17 38.916306568179266 41.0 41.0 41.0 37.0 41.0 18-19 38.84551860611241 41.0 41.0 41.0 37.0 41.0 20-21 38.854467850012725 41.0 41.0 41.0 37.0 41.0 22-23 38.84942039930429 41.0 41.0 41.0 34.5 41.0 24-25 38.86660376193983 41.0 41.0 41.0 37.0 41.0 26-27 38.8315752726562 41.0 41.0 41.0 37.0 41.0 28-29 38.788522466116746 41.0 41.0 41.0 34.5 41.0 30-31 38.65361197141385 41.0 41.0 41.0 32.0 41.0 32-33 38.70560287815913 41.0 41.0 41.0 32.0 41.0 34-35 38.67594286370662 41.0 41.0 41.0 32.0 41.0 36-37 38.60267178647996 41.0 41.0 41.0 32.0 41.0 38-39 38.498080284318505 41.0 41.0 41.0 32.0 41.0 40-41 38.55071088198913 41.0 41.0 41.0 32.0 41.0 42-43 38.28298233552978 41.0 41.0 41.0 32.0 41.0 44-45 38.403388883221794 41.0 41.0 41.0 32.0 41.0 46-47 38.41320819250132 41.0 41.0 41.0 32.0 41.0 48-49 38.36337744807681 41.0 41.0 41.0 32.0 41.0 50-51 38.227335225475514 41.0 41.0 41.0 32.0 41.0 52-53 38.21629675315602 41.0 41.0 41.0 32.0 41.0 54-55 38.06459828267096 41.0 37.0 41.0 32.0 41.0 56-57 37.870196317609015 41.0 37.0 41.0 27.0 41.0 58-59 37.87117674842424 41.0 37.0 41.0 29.5 41.0 60-61 37.866633764194276 41.0 37.0 41.0 29.5 41.0 62-63 37.86503914436999 41.0 37.0 41.0 29.5 41.0 64-65 37.857221842669894 41.0 37.0 41.0 29.5 41.0 66-67 37.71711710051355 41.0 37.0 41.0 27.0 41.0 68-69 37.7524696141453 41.0 37.0 41.0 27.0 41.0 70-71 37.7239610433584 41.0 37.0 41.0 27.0 41.0 72-73 37.575057497177646 41.0 37.0 41.0 27.0 41.0 74-75 37.51524371688501 41.0 37.0 41.0 27.0 41.0 76-77 36.43859031407217 39.0 34.5 41.0 24.5 41.0 78-79 36.97214397824751 41.0 37.0 41.0 27.0 41.0 80-81 37.62860444942005 41.0 37.0 41.0 27.0 41.0 82-83 37.803200640505274 41.0 37.0 41.0 27.0 41.0 84-85 37.72454865894208 41.0 37.0 41.0 27.0 41.0 86-87 37.582641852802084 41.0 37.0 41.0 27.0 41.0 88-89 37.506230182439424 41.0 37.0 41.0 27.0 41.0 90-91 37.338935897183134 41.0 37.0 41.0 27.0 41.0 92-93 37.217537132075925 41.0 37.0 41.0 27.0 41.0 94-95 37.06927370542415 41.0 37.0 41.0 27.0 41.0 96-97 37.00949228470597 41.0 37.0 41.0 27.0 41.0 98-99 36.88584742224916 41.0 37.0 41.0 27.0 41.0 100 36.747093853052384 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3144380934334023 1101 2 -0.3580252430494575 1101 3 -0.019586438070659185 1101 4 0.20053772563257333 1101 5 -1.1024892455934108 1101 6 -0.6339017524389341 1101 7 -0.09467416301769305 1101 8 0.042578861646809685 1101 9 -0.18817845276213063 1101 10-11 -0.09793840318755542 1101 12-13 -0.1023250682561283 1101 14-15 -0.03101629103034753 1101 16-17 -0.0601940417873692 1101 18-19 0.14567827155153878 1101 20-21 0.11562786381158219 1101 22-23 0.06300232024847219 1101 24-25 0.009145484878160914 1101 26-27 0.13376491247711897 1101 28-29 0.06095969634045417 1101 30-31 -0.20200018481489224 1101 32-33 -0.2342982688954791 1101 34-35 -0.033409670450659235 1101 36-37 -0.05765006909536652 1101 38-39 -0.21748626431097762 1101 40-41 0.01573877983059191 1101 42-43 0.008532556969008454 1101 44-45 -0.055565829192133265 1101 46-47 0.0953264922683772 1101 48-49 0.24208371772930093 1101 50-51 0.15788352033955277 1101 52-53 0.18652346592527635 1101 54-55 0.0400764005997587 1101 56-57 0.07876748747663953 1101 58-59 0.06660796631037158 1101 60-61 -0.08850118098675352 1101 62-63 -0.10327732369228926 1101 64-65 -0.03266825208429225 1101 66-67 0.1688697829601864 1101 68-69 -0.011221510026409476 1101 70-71 0.04170289993651721 1101 72-73 0.1851203297752093 1101 74-75 0.1180838588012918 1101 76-77 -0.32349017989064066 1101 78-79 -0.05590128882172962 1101 80-81 -0.14750665485716752 1101 82-83 -0.16845717582046404 1101 84-85 0.06080814389805056 1101 86-87 -0.01947889588694096 1101 88-89 -0.2867503956851749 1101 90-91 -0.16343756386642383 1101 92-93 -0.07082618315886435 1101 94-95 -0.07201091866358666 1101 96-97 -0.14899980673268232 1101 98-99 -0.4176854105015124 1101 100 -0.5193784190970305 1102 1 1.8005996474450487 1102 2 0.8970249814629909 1102 3 -0.04832702245819043 1102 4 0.21850133268214122 1102 5 -0.793568614470832 1102 6 -0.017451052600065964 1102 7 0.10130398298139198 1102 8 -0.12357245661014815 1102 9 -0.12929390314958056 1102 10-11 -0.1274885664563854 1102 12-13 -0.28050790845966844 1102 14-15 -0.17401348711721454 1102 16-17 -0.17955393206160153 1102 18-19 -0.14195735457185066 1102 20-21 -0.04631485489221632 1102 22-23 -0.1697464451466999 1102 24-25 -0.10665005069964195 1102 26-27 0.01666094740402002 1102 28-29 -0.2921744338245489 1102 30-31 -0.29782436721261973 1102 32-33 -0.2741664384519922 1102 34-35 -0.09058640148484187 1102 36-37 -0.3419816258890904 1102 38-39 -0.21949543471161803 1102 40-41 -0.21956880554727576 1102 42-43 -0.1080325592371949 1102 44-45 0.04833752553373927 1102 46-47 -0.33213186919429205 1102 48-49 -0.2310911038333927 1102 50-51 -0.21967007731932853 1102 52-53 -0.3311091170075997 1102 54-55 -0.24216966865205336 1102 56-57 -0.09708868877584109 1102 58-59 -0.07591506651145608 1102 60-61 -0.055252544250649294 1102 62-63 -0.2530993848770393 1102 64-65 -0.2561128541479576 1102 66-67 -0.1815125386191241 1102 68-69 -0.38234630837349215 1102 70-71 -0.2991609056563007 1102 72-73 -0.1279659354181888 1102 74-75 -0.28789817070768464 1102 76-77 -0.26125942629032295 1102 78-79 -0.25613551004506263 1102 80-81 -0.25271108034932155 1102 82-83 -0.2574392831895409 1102 84-85 -0.33969910990973773 1102 86-87 -0.19290111317168623 1102 88-89 -0.3284178931760877 1102 90-91 -0.42312082043159904 1102 92-93 -0.3968185908495485 1102 94-95 -0.2990528710151068 1102 96-97 -0.2605500678013968 1102 98-99 -0.4203267194627358 1102 100 -0.3537756822195419 1103 1 1.5601580157906305 1103 2 0.6244227668583324 1103 3 0.22068539101997509 1103 4 1.0066570557453467 1103 5 2.891176477663297 1103 6 -0.13057136974263273 1103 7 -0.4061999069950204 1103 8 -0.20833014626433766 1103 9 -0.25517511444049745 1103 10-11 -0.3744266555503799 1103 12-13 -0.46223414761767856 1103 14-15 -0.42868244298947644 1103 16-17 -0.3000952219098636 1103 18-19 -0.258365821286354 1103 20-21 -0.3169874591243982 1103 22-23 -0.2585716329038732 1103 24-25 -0.35415748864946295 1103 26-27 -0.28902796471155057 1103 28-29 -0.22803386518429392 1103 30-31 -0.28149421672146957 1103 32-33 -0.4333461922688926 1103 34-35 -0.43735700753381224 1103 36-37 -0.3930208966895137 1103 38-39 -0.24453718860134188 1103 40-41 -0.23065892899312956 1103 42-43 -0.04010928994453877 1103 44-45 -0.4324428908339826 1103 46-47 -0.5109286928477133 1103 48-49 -0.3072284729003769 1103 50-51 -0.37685477271030976 1103 52-53 -0.3403666949572113 1103 54-55 -0.27090800733441256 1103 56-57 -0.1943010691855136 1103 58-59 -0.5101347288782421 1103 60-61 -0.28933734918100384 1103 62-63 -0.3714614513820962 1103 64-65 -0.556624480773209 1103 66-67 -0.47464132956229577 1103 68-69 -0.4475969104463715 1103 70-71 -0.4838321841963307 1103 72-73 -0.48572750865160685 1103 74-75 -0.3773825138601907 1103 76-77 -0.5925674376241545 1103 78-79 -0.46877992634192367 1103 80-81 -0.33404480341843623 1103 82-83 -0.41946442803642014 1103 84-85 -0.46412705455544767 1103 86-87 -0.2956401634170973 1103 88-89 -0.25718580650515577 1103 90-91 -0.6249153765314048 1103 92-93 -0.3350051187860217 1103 94-95 -0.6446690324523203 1103 96-97 -0.21390356700865 1103 98-99 -0.31574503586323033 1103 100 -0.29085918901482444 1104 1 1.4654413034379967 1104 2 -0.49042002490572045 1104 3 -0.06038601669189525 1104 4 0.2128856419948093 1104 5 -1.117893399593406 1104 6 -0.2604190072880854 1104 7 -0.297282184304386 1104 8 -0.18293112413213208 1104 9 -0.13871945457772483 1104 10-11 -0.04129712717013234 1104 12-13 -0.03291313613134861 1104 14-15 -0.040248516382028754 1104 16-17 0.007026591828001472 1104 18-19 -0.04634036385746043 1104 20-21 -0.023358721215345213 1104 22-23 -0.06533793259788467 1104 24-25 -0.14547148736451732 1104 26-27 -0.09942649682091087 1104 28-29 -0.06993764845758932 1104 30-31 -0.28189477326861834 1104 32-33 -0.1082638040856736 1104 34-35 -0.3055856591196573 1104 36-37 -0.022339779793824732 1104 38-39 -0.02000699181014909 1104 40-41 -0.044185563961754326 1104 42-43 -0.09585705984564896 1104 44-45 -0.0017454843912148021 1104 46-47 -0.22165620847151501 1104 48-49 -0.3806609937857388 1104 50-51 -0.21122593976652126 1104 52-53 -0.04042235462199528 1104 54-55 -0.16270675742969587 1104 56-57 -0.21537019507385935 1104 58-59 -0.2106635823138916 1104 60-61 -0.28777694817403443 1104 62-63 -0.1595290487951715 1104 64-65 -0.3276786337415132 1104 66-67 -0.6089756459111371 1104 68-69 -0.5337415641982162 1104 70-71 -0.17466093655858828 1104 72-73 -0.16200320143995128 1104 74-75 -0.4359208153321461 1104 76-77 -0.5799628375471428 1104 78-79 -0.40556722772181075 1104 80-81 -0.522226937482742 1104 82-83 -0.19361896540041101 1104 84-85 -0.15993733454163106 1104 86-87 -0.3204898897467672 1104 88-89 -0.3867958572200365 1104 90-91 -0.3207319674177711 1104 92-93 -0.4542801423714664 1104 94-95 -0.28991254410447453 1104 96-97 -0.4587786210843987 1104 98-99 -0.20450304575673783 1104 100 -0.07237310219861826 1105 1 1.4958915542817017 1105 2 0.4672305255035081 1105 3 0.06422282430861337 1105 4 -0.16516736592628511 1105 5 -1.1997566537770616 1105 6 0.26761306262436335 1105 7 0.13160458525427288 1105 8 -0.12670061138539523 1105 9 -0.12635130099430825 1105 10-11 -0.15698569308503352 1105 12-13 -0.22269432746044515 1105 14-15 -0.2661929596089223 1105 16-17 -0.23790074255965976 1105 18-19 -0.26702544324632527 1105 20-21 -0.10133542382185823 1105 22-23 -0.0010409253545304864 1105 24-25 -0.19040784736535699 1105 26-27 -0.08905964924136356 1105 28-29 -0.1803376292280845 1105 30-31 -0.026192199979107045 1105 32-33 -0.2329361542964321 1105 34-35 0.011149265219550841 1105 36-37 -0.28184406860967215 1105 38-39 -0.2162167527162353 1105 40-41 -0.24526503201143868 1105 42-43 -0.17182927509639256 1105 44-45 -0.23847235401871814 1105 46-47 -0.33513550223025135 1105 48-49 -0.30075603844132104 1105 50-51 -0.19931036294853754 1105 52-53 -0.2646425051164414 1105 54-55 -0.11067904620501423 1105 56-57 -0.16884999313578675 1105 58-59 -0.3044682176143141 1105 60-61 -0.2746946400018899 1105 62-63 -0.42862876626755764 1105 64-65 -0.3209212066398379 1105 66-67 -0.08088807908212914 1105 68-69 -0.30246988829266996 1105 70-71 -0.27682887470556494 1105 72-73 -0.3041520137705973 1105 74-75 -0.2918823977978562 1105 76-77 -0.4696839838047282 1105 78-79 -0.32925413650585256 1105 80-81 -0.3512391701342992 1105 82-83 -0.35339447035755 1105 84-85 -0.10532436249675214 1105 86-87 -0.2599261400498847 1105 88-89 -0.24564127627360932 1105 90-91 -0.23287245403264478 1105 92-93 -0.21967440966959373 1105 94-95 -0.28417621313641206 1105 96-97 -0.2513072279185522 1105 98-99 -0.2949535935266283 1105 100 -0.2571210413306346 1106 1 1.3479391424104357 1106 2 -0.6805928263245136 1106 3 -0.06203856717963063 1106 4 -0.26898429907412336 1106 5 -1.1259197339536726 1106 6 -0.4515588655702949 1106 7 -0.28517448981071425 1106 8 0.028391947468570322 1106 9 -0.06272422951008849 1106 10-11 -0.12392869086069425 1106 12-13 -0.026399996275920046 1106 14-15 0.047448334339080134 1106 16-17 0.03375442781135263 1106 18-19 0.015983837322686156 1106 20-21 0.23730896145504232 1106 22-23 0.09248705434566062 1106 24-25 -0.05347396607145072 1106 26-27 -0.09389740266568936 1106 28-29 0.006715626728770019 1106 30-31 0.177061147471683 1106 32-33 -0.04929864046772536 1106 34-35 -0.02653391658773785 1106 36-37 -0.0887106506154538 1106 38-39 2.693631030439292E-4 1106 40-41 0.13644473851557137 1106 42-43 0.05415638866011108 1106 44-45 0.08272812065895607 1106 46-47 -0.060708126831961806 1106 48-49 0.05050249680006402 1106 50-51 -0.12381587961890972 1106 52-53 -0.034276910220164325 1106 54-55 -0.20578795266445127 1106 56-57 -0.20306124820591265 1106 58-59 -0.09393673001982705 1106 60-61 -0.035011450671980526 1106 62-63 -0.41974254738831007 1106 64-65 -0.4786042520995295 1106 66-67 -0.24403701719302262 1106 68-69 -0.34120553241982776 1106 70-71 -0.420399827403763 1106 72-73 -0.3862098702830963 1106 74-75 -0.5152224482088386 1106 76-77 -0.2816328045626548 1106 78-79 0.026897631522707854 1106 80-81 -0.17482102593779558 1106 82-83 -0.18336127207101072 1106 84-85 -0.5181183267182163 1106 86-87 -0.24832166747290785 1106 88-89 -0.38776612176592806 1106 90-91 -0.2815448987025633 1106 92-93 -0.2455556499812772 1106 94-95 -0.2173180564711572 1106 96-97 -0.25244361452884334 1106 98-99 -0.48189118911686535 1106 100 -0.49414580534352126 1107 1 1.4951193999151169 1107 2 -0.44746377008091187 1107 3 -0.11841439381136887 1107 4 -0.3524072296823846 1107 5 -1.2834183565276795 1107 6 -0.3426602474115725 1107 7 -0.4400838333386474 1107 8 -0.5680365618911196 1107 9 -0.2420228202377217 1107 10-11 -0.21895323558206314 1107 12-13 -0.3368618344127441 1107 14-15 -0.35205710749173846 1107 16-17 -0.37989329214349965 1107 18-19 -0.4277360395567982 1107 20-21 -0.3188846175086866 1107 22-23 -0.2543740583702956 1107 24-25 -0.23759789260684983 1107 26-27 -0.40694581901330196 1107 28-29 -0.4631166302629808 1107 30-31 -0.536193753604941 1107 32-33 -0.45961482138329046 1107 34-35 -0.4328278491964923 1107 36-37 -0.2985403054111302 1107 38-39 -0.412091584979386 1107 40-41 -0.416317605358536 1107 42-43 -0.3373849944775671 1107 44-45 -0.38889349842369114 1107 46-47 -0.3846473679019482 1107 48-49 -0.4233396785982819 1107 50-51 -0.6194781032940995 1107 52-53 -0.38808797669312867 1107 54-55 -0.6381269793585744 1107 56-57 -0.35475448404613985 1107 58-59 -0.24832234635748307 1107 60-61 -0.46459045855889514 1107 62-63 -0.6338160364118792 1107 64-65 -0.5339043242940278 1107 66-67 -0.6827803114887132 1107 68-69 -0.7519995263258039 1107 70-71 -0.42776700149867253 1107 72-73 -0.586604450575642 1107 74-75 -0.5359452527937805 1107 76-77 -0.5296997110635004 1107 78-79 -0.7640054620375096 1107 80-81 -0.6180087208382901 1107 82-83 -0.3870909142314787 1107 84-85 -0.4373025241544397 1107 86-87 -0.4314783426929978 1107 88-89 -0.35296374540664743 1107 90-91 -0.23440628103233507 1107 92-93 -0.3682422671310661 1107 94-95 -0.40118916133825167 1107 96-97 -0.4408827712857928 1107 98-99 -0.6387168339941312 1107 100 -0.6922194287042913 1108 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16-17 0.1088522651309276 2223 18-19 0.16290699818626564 2223 20-21 0.1076801090522963 2223 22-23 0.11073758503617626 2223 24-25 0.1492654322430269 2223 26-27 0.15228936681876348 2223 28-29 -1.2086090212903855E-4 2223 30-31 0.013327735047745648 2223 32-33 0.0971924599736127 2223 34-35 0.1499908626487425 2223 36-37 0.028167406217860957 2223 38-39 0.11991232683701014 2223 40-41 0.09475801939542094 2223 42-43 0.18704306525041403 2223 44-45 0.19028924799855673 2223 46-47 0.20213756813944883 2223 48-49 0.04312086223598044 2223 50-51 0.05233443170708796 2223 52-53 0.07291930909958211 2223 54-55 0.08752674587721287 2223 56-57 0.3087696993517852 2223 58-59 0.4113212098190786 2223 60-61 0.23066490459407163 2223 62-63 0.15649944562037632 2223 64-65 0.2738493754359368 2223 66-67 0.32194138398289596 2223 68-69 0.2974166122040671 2223 70-71 0.19007473489315174 2223 72-73 0.4039642876071241 2223 74-75 0.23744732612177444 2223 76-77 0.26366988489105836 2223 78-79 0.4894548043319489 2223 80-81 0.24940121866855947 2223 82-83 0.0904521822858726 2223 84-85 0.12378425939542126 2223 86-87 0.29858172073886635 2223 88-89 0.3453187010449952 2223 90-91 0.31740206768766654 2223 92-93 0.32446355374045766 2223 94-95 0.5181098722129889 2223 96-97 0.38007885354578974 2223 98-99 0.41599938604345965 2223 100 0.4315038941494791 2224 1 1.3654100126461284 2224 2 -0.9052728764542639 2224 3 -0.03829681925400408 2224 4 -0.34933399601275994 2224 5 -1.0036283012025464 2224 6 0.48057122951782816 2224 7 0.4889989050821981 2224 8 0.38217520618187706 2224 9 0.2801190032065577 2224 10-11 0.23757995372590557 2224 12-13 0.29268471647967687 2224 14-15 0.3315507742043451 2224 16-17 0.2963909917803633 2224 18-19 0.11888167233244218 2224 20-21 0.09217890829701503 2224 22-23 0.3399801009393073 2224 24-25 0.26577054691057356 2224 26-27 0.2647483486516151 2224 28-29 0.15630913781462397 2224 30-31 0.34380033766191076 2224 32-33 0.2816110560981997 2224 34-35 0.20973141701271203 2224 36-37 0.033559118256576426 2224 38-39 0.25617032423384956 2224 40-41 0.1848192487488447 2224 42-43 0.4693805049834978 2224 44-45 0.3205856329002117 2224 46-47 0.38457170992022327 2224 48-49 0.27311912068251587 2224 50-51 0.33295117078571224 2224 52-53 0.2941740525412513 2224 54-55 0.32159565639803134 2224 56-57 0.4389920682311441 2224 58-59 0.5445550912834562 2224 60-61 0.4167104782984481 2224 62-63 0.49667960474337036 2224 64-65 0.27423045483244834 2224 66-67 0.32527714632500704 2224 68-69 0.37130430950373494 2224 70-71 0.3231711252944365 2224 72-73 0.41451924736806944 2224 74-75 0.4309875376746888 2224 76-77 0.5991343297021459 2224 78-79 0.41535101691146536 2224 80-81 0.3528870754212008 2224 82-83 0.31689217201986963 2224 84-85 0.24907184486902878 2224 86-87 0.4333928538485452 2224 88-89 0.3662941084768434 2224 90-91 0.46463643495378903 2224 92-93 0.5134255420445513 2224 94-95 0.4826206790105516 2224 96-97 0.376608579113622 2224 98-99 0.48057206381940887 2224 100 0.5562234123807883 2225 1 1.466626350250852 2225 2 1.5532598189205373 2225 3 0.7703247870082546 2225 4 0.866077835551522 2225 5 3.169658560151465 2225 6 -0.041209188088362936 2225 7 0.11918323476743353 2225 8 0.27676676606381534 2225 9 0.10368222159736717 2225 10-11 0.06911627886476879 2225 12-13 0.09019429502374265 2225 14-15 0.1740756142564095 2225 16-17 0.08706107739146063 2225 18-19 0.1796694502935381 2225 20-21 0.023149457711298282 2225 22-23 0.165335286185595 2225 24-25 0.09778075791352592 2225 26-27 0.16593879210611817 2225 28-29 0.08584657204806234 2225 30-31 0.015722371063070284 2225 32-33 0.1640028048011999 2225 34-35 0.18040274004337675 2225 36-37 0.1227555288232196 2225 38-39 0.16062113909755027 2225 40-41 0.24466223395480569 2225 42-43 0.33527103459906726 2225 44-45 0.22596932462691655 2225 46-47 0.12838277886741167 2225 48-49 0.08478752890265184 2225 50-51 0.2230719795998084 2225 52-53 0.17301509454019026 2225 54-55 0.28532368074311876 2225 56-57 0.4320934541410608 2225 58-59 0.3378058841485796 2225 60-61 0.29340436819560267 2225 62-63 0.49486151458589944 2225 64-65 0.451052440570038 2225 66-67 0.5120754835997516 2225 68-69 0.5273016696753317 2225 70-71 0.2549693709084764 2225 72-73 0.35535317649601694 2225 74-75 0.2278687820604759 2225 76-77 0.6039476626688298 2225 78-79 0.5105276012668227 2225 80-81 0.5228686516187508 2225 82-83 0.30668781446978244 2225 84-85 0.11947391456782697 2225 86-87 0.36826562878484026 2225 88-89 0.4600217661790964 2225 90-91 0.5353139450823008 2225 92-93 0.5471647031657412 2225 94-95 0.5652842017149098 2225 96-97 0.4921478190630282 2225 98-99 0.48496490328484043 2225 100 0.45976059913031975 2226 1 1.3856943983864074 2226 2 1.6166401006106774 2226 3 1.0917321584399104 2226 4 0.8569579823325917 2226 5 3.0473297597629276 2226 6 -0.4928793623908838 2226 7 -0.04362907973258956 2226 8 0.04407964718193824 2226 9 0.039743416352102656 2226 10-11 -0.08610874946613478 2226 12-13 0.14714481037639615 2226 14-15 0.11895109318361108 2226 16-17 0.022737106747676705 2226 18-19 -0.09989654722412666 2226 20-21 -0.02018266144038705 2226 22-23 0.1696361873781953 2226 24-25 0.08372997813908967 2226 26-27 -0.1027225051795071 2226 28-29 0.0610118356603877 2226 30-31 0.10297211473583445 2226 32-33 0.015329635517296936 2226 34-35 -0.149064099306905 2226 36-37 0.006637942076693548 2226 38-39 -0.05503438407982486 2226 40-41 -0.0393793720027773 2226 42-43 0.04093519195701134 2226 44-45 0.029588935009520867 2226 46-47 -0.09315140709125558 2226 48-49 0.04669999989719997 2226 50-51 -0.12242205741924295 2226 52-53 0.029900853538627814 2226 54-55 0.12310631524746185 2226 56-57 0.23128602334941206 2226 58-59 0.2036381382093495 2226 60-61 0.04758520031475655 2226 62-63 0.04899108125795948 2226 64-65 0.1279458893855363 2226 66-67 0.0049961300840237755 2226 68-69 0.2124077082053546 2226 70-71 0.08636608452349748 2226 72-73 0.10431977300996209 2226 74-75 0.31302473694687905 2226 76-77 0.45382691905003725 2226 78-79 0.07775751330610348 2226 80-81 0.045877393726570403 2226 82-83 -0.03351441422807966 2226 84-85 0.06269136100893036 2226 86-87 0.17175572645901127 2226 88-89 0.24490115746936425 2226 90-91 0.18271492182397253 2226 92-93 -0.00978918491369285 2226 94-95 0.3027949944406103 2226 96-97 0.4050505226408134 2226 98-99 0.36768709852525205 2226 100 0.3469685209909841 2227 1 1.4118723388947334 2227 2 1.6299517860299595 2227 3 0.7844654080323412 2227 4 0.05862656434967839 2227 5 3.0550022972013444 2227 6 0.16145244757072064 2227 7 0.3763689603739522 2227 8 0.18949750537473875 2227 9 0.29036568252345063 2227 10-11 0.21066789394906493 2227 12-13 0.18722930851004804 2227 14-15 0.1903141545371838 2227 16-17 0.19545538988101185 2227 18-19 0.17138455494868055 2227 20-21 0.0049879383760895735 2227 22-23 0.20118757667539455 2227 24-25 0.26452012700190863 2227 26-27 0.24592917721085428 2227 28-29 0.2790000941168884 2227 30-31 0.27702668885989823 2227 32-33 0.15380947088625163 2227 34-35 0.2199992052516322 2227 36-37 -0.024874917496944704 2227 38-39 0.13712871468989363 2227 40-41 0.17885654571733056 2227 42-43 0.4085100910670363 2227 44-45 0.34479325390200444 2227 46-47 0.2535481768768193 2227 48-49 0.25610169787761805 2227 50-51 0.1704036189667164 2227 52-53 0.11453417529077115 2227 54-55 0.21135987200864292 2227 56-57 0.3147694667040355 2227 58-59 0.45358312756356156 2227 60-61 0.3800256685511201 2227 62-63 0.4180105109289656 2227 64-65 0.2911475535132553 2227 66-67 0.1863709062552772 2227 68-69 0.21831455981470071 2227 70-71 0.30379131904256695 2227 72-73 0.3698554920681474 2227 74-75 0.23957750707810277 2227 76-77 0.4793752437865635 2227 78-79 0.3474865134931875 2227 80-81 0.34824106977399794 2227 82-83 -0.01979649634761671 2227 84-85 0.22720481745921006 2227 86-87 0.21416648449234543 2227 88-89 0.36827221875167737 2227 90-91 0.4244695609808673 2227 92-93 0.3065819387288613 2227 94-95 0.474123089513256 2227 96-97 0.45312025030471403 2227 98-99 0.4169808687366512 2227 100 0.526208795019727 2228 1 1.3119431020095718 2228 2 1.6051204681683586 2228 3 -0.1858095765304828 2228 4 -0.13121013962162031 2228 5 3.083700796752879 2228 6 0.03975476382325027 2228 7 0.3791715402979605 2228 8 0.4205106799342744 2228 9 0.2786938250592925 2228 10-11 0.05308643749091857 2228 12-13 0.17522628417564334 2228 14-15 0.005095194529609159 2228 16-17 0.04287862091582184 2228 18-19 0.05644148094488344 2228 20-21 0.09265453593228301 2228 22-23 0.16014057554409078 2228 24-25 0.14045942847531023 2228 26-27 0.10019141539859788 2228 28-29 0.2068762541587077 2228 30-31 -0.021424860942467205 2228 32-33 0.022938949357588 2228 34-35 0.17020033926434763 2228 36-37 0.07426420639259845 2228 38-39 0.09669300320508967 2228 40-41 0.012186874247483104 2228 42-43 0.29973884523057137 2228 44-45 0.28979769349869144 2228 46-47 0.1252694107691994 2228 48-49 0.14866435929188526 2228 50-51 0.004665248681817502 2228 52-53 0.053247345793820955 2228 54-55 0.14428883395432024 2228 56-57 0.2366321141459764 2228 58-59 0.41911213818704596 2228 60-61 0.36966709787589735 2228 62-63 0.3659058716630952 2228 64-65 0.32100846117414505 2228 66-67 0.437504242893894 2228 68-69 0.4144545896524008 2228 70-71 0.575327644859378 2228 72-73 0.4985053138082023 2228 74-75 0.464878137636056 2228 76-77 0.517245116277735 2228 78-79 0.5258188026574189 2228 80-81 0.5859457532104742 2228 82-83 0.34755736880583754 2228 84-85 0.3154630983211675 2228 86-87 0.3136267467215177 2228 88-89 0.43860996614087355 2228 90-91 0.34144887741818764 2228 92-93 0.4816653220170508 2228 94-95 0.7936606953762251 2228 96-97 0.4414092928392037 2228 98-99 0.4798395858960305 2228 100 0.5451106596503337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 20.0 13 37.0 14 111.0 15 412.0 16 1482.0 17 3856.0 18 6407.0 19 8242.0 20 9532.0 21 11064.0 22 12643.0 23 14670.0 24 16495.0 25 18583.0 26 21468.0 27 23587.0 28 26847.0 29 30493.0 30 34599.0 31 40475.0 32 47019.0 33 56483.0 34 69084.0 35 89087.0 36 125405.0 37 207614.0 38 509685.0 39 946698.0 40 1060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.668902909791107 18.270784757449725 21.381397815517435 34.67891451724173 2 19.234862639352702 53.09841025726752 10.757649209029159 16.909077894350624 3 41.38060945722493 14.626570510869815 23.94042752355391 20.052392508351343 4 18.80318435356714 15.947141170893698 21.790380248572962 43.4592942269662 5 17.044837940679542 35.21214594125215 26.50266291438471 21.24035320368359 6 17.358490080826073 27.633147862253647 31.26847817421709 23.739883882703186 7 20.305954418860615 26.954282564661288 36.52851628565232 16.211246730825774 8 20.447779361706324 27.43903327592902 34.813887443542185 17.299299918822474 9 20.708670394375346 27.783116274165742 28.150043846151867 23.35816948530704 10-11 19.345753695206238 30.0749884919924 28.82031564086101 21.758942171940348 12-13 24.15408643563788 26.645687947408618 27.29523675636198 21.904988860591523 14-15 21.771264526448704 27.308052005050666 28.548152332589567 22.37253113591107 16-17 20.95991784525523 29.677951514642388 28.207112420161856 21.155018219940526 18-19 21.4768352593352 29.551170559387746 29.844978351230395 19.12701583004666 20-21 22.811699850588774 28.96694094441954 29.91100474121341 18.31035446377828 22-23 22.134334665719166 30.357073974415794 28.51669282577519 18.991898534089845 24-25 22.11654761486363 28.198776079459687 26.688291148734887 22.99638515694179 26-27 20.130034056844842 26.49732679912805 30.07398127344998 23.298657870577134 28-29 19.7055609619362 27.953664329552964 30.701024816894424 21.639749891616415 30-31 20.00250329736558 30.17881430291527 28.151936433392788 21.666745966326364 32-33 20.63514344077855 30.131199858732245 28.79556378093554 20.438092919553668 34-35 21.4630556524676 29.140268254443118 29.882138286391235 19.514537806698044 36-37 21.880344151574818 28.195025797652796 28.787613183504934 21.137016867267455 38-39 21.462116059094637 29.27948141966681 28.948189542071862 20.310212979166696 40-41 20.754473563015416 29.888023501542033 29.751811252169034 19.60569168327352 42-43 20.506220009767887 30.0438826301976 29.318063026660674 20.131834333373835 44-45 20.499940424094724 30.202819526152965 28.747131570172275 20.550108479580036 46-47 19.813553582985797 27.834821332899008 29.152847486830886 23.198777597284312 48-49 20.75620253750496 28.261352210180362 30.325314445056872 20.657130807257804 50-51 21.9165611587385 30.7675691059071 28.06890917803252 19.24696055732188 52-53 20.275639191684107 30.26692769025201 28.40365218714405 21.053780930919828 54-55 22.62377846253037 28.220430605354448 28.10599318562568 21.0497977464895 56-57 21.477422609839223 28.89271962165334 29.187483148562276 20.44237461994516 58-59 21.31829334997711 27.869722445606232 28.97971711296791 21.832267091448745 60-61 19.477998228148778 30.449096137287178 29.69927390102578 20.373631733538264 62-63 20.284742586306447 31.291978709568735 27.3812315169368 21.04204718718802 64-65 19.966898919982437 30.269143968696483 28.86017290310359 20.903784208217495 66-67 20.22728422164294 30.188054130924698 29.504859936618093 20.07980171081427 68-69 20.715742354354056 30.151065637217883 29.276885663122687 19.856306345305374 70-71 19.604908883153218 30.81970873811375 29.397151843124213 20.17823053560882 72-73 19.94890616066293 30.803528950889735 28.6304013701601 20.61716351828723 74-75 20.015361154281024 29.18979340447582 30.452588294491846 20.342257146751315 76-77 19.577906854143613 30.373425203093834 28.720022390253895 21.32864555250866 78-79 19.545182966605775 29.546455919401943 28.56694660198752 22.34141451200476 80-81 21.106639101696867 29.252863451605972 28.19800403273765 21.44249341395951 82-83 20.260372422270585 27.89663194691487 30.104219259904387 21.738776370910156 84-85 20.679911090461943 29.117209378876186 28.550445362037202 21.65243416862467 86-87 22.046599501619692 28.57699735722999 29.31820305354374 20.058200087606583 88-89 22.028791020582403 27.959593820907113 28.87303388797501 21.13858127053547 90-91 21.436996551455152 28.475375435354145 28.87142662434349 21.21620138884722 92-93 21.8919593100853 28.30770569331353 29.457306363306728 20.343028633294445 94-95 21.250382528744304 28.322063915088474 29.0877000186014 21.339853537565823 96-97 20.66167400578958 29.029838527866524 29.6305908129668 20.677896653377097 98-99 22.392354054033202 29.339697525842656 28.304941199867308 19.963007220256838 100 21.82462567901531 30.91732321600166 27.380400298651015 19.877650806332017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 3.5 2 7.0 3 14.5 4 22.0 5 22.0 6 24.5 7 37.5 8 52.5 9 54.0 10 58.0 11 79.0 12 92.0 13 104.0 14 126.0 15 169.5 16 227.0 17 313.0 18 443.5 19 668.0 20 1025.5 21 1612.5 22 2489.0 23 3805.0 24 5836.0 25 8726.5 26 12590.5 27 17017.0 28 21825.0 29 27525.0 30 34062.0 31 41139.0 32 49320.5 33 58787.0 34 68941.5 35 80026.0 36 91115.5 37 99483.5 38 105396.0 39 110212.0 40 113280.5 41 114098.0 42 112281.5 43 110181.5 44 109394.5 45 106331.0 46 99224.0 47 91167.5 48 84874.0 49 81027.5 50 77460.0 51 71109.5 52 62419.0 53 53214.0 54 44214.0 55 35540.5 56 27609.5 57 20226.5 58 14094.0 59 10280.5 60 8168.0 61 6781.5 62 5570.0 63 4403.5 64 3342.0 65 3102.5 66 3831.0 67 3597.0 68 2321.5 69 1962.0 70 2539.0 71 2519.0 72 1569.0 73 699.0 74 284.5 75 157.5 76 101.5 77 68.5 78 64.0 79 55.0 80 43.0 81 30.0 82 25.0 83 27.0 84 20.5 85 20.0 86 22.5 87 20.5 88 14.5 89 9.0 90 13.5 91 15.5 92 14.0 93 12.5 94 11.0 95 10.0 96 13.5 97 21.0 98 38.0 99 63.0 100 66.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.343380945482474 2 0.0059575905275167824 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.071734533194923E-4 30-31 0.01007218542421902 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.143469066389846E-4 40-41 0.0014786825410023669 42-43 1.0715090876828744E-4 44-45 0.0 46-47 0.002057297448351119 48-49 0.0 50-51 0.0 52-53 0.0015215429045096819 54-55 0.01894428067023322 56-57 0.01161515851048236 58-59 0.015172568681589503 60-61 2.143018175365749E-4 62-63 0.01195804141854088 64-65 6.429054526097246E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5001127227560243E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2333158.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.11396800849897 #Duplication Level Percentage of deduplicated Percentage of total 1 86.53351194056431 64.9987544756661 2 8.470315362869135 12.72477994376899 3 2.264351558004918 5.102532914639286 4 0.9699527828180019 2.914280091933837 5 0.5117166255452639 1.9218533120311996 6 0.30475077577839965 1.3734624013430372 7 0.2159327098573812 1.1353693862151073 8 0.1442409923433845 0.8667610627516102 9 0.10086081709920541 0.6818450570010703 >10 0.45265258942071196 5.7541388896126415 >50 0.022518656858782217 1.1484021620101723 >100 0.008672579124626724 1.1329109136603395 >500 5.226097158869599E-4 0.24490938936670817 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.286036350731498E-5 0.0 2 0.0 0.0 0.0 4.286036350731498E-5 0.0 3 0.0 0.0 0.0 4.286036350731498E-5 0.0 4 0.0 0.0 0.0 4.286036350731498E-5 0.0 5 0.0 0.0 0.0 4.286036350731498E-5 0.0 6 0.0 0.0 0.0 4.286036350731498E-5 0.0 7 0.0 0.0 0.0 4.286036350731498E-5 0.0 8 0.0 0.0 0.0 4.286036350731498E-5 0.0 9 0.0 0.0 0.0 4.286036350731498E-5 0.0 10-11 0.0 0.0 0.0 4.286036350731498E-5 0.0 12-13 0.0 0.0 0.0 4.286036350731498E-5 0.0 14-15 0.0 0.0 0.0 4.286036350731498E-5 0.0 16-17 0.0 0.0 0.0 4.286036350731498E-5 0.0 18-19 0.0 0.0 0.0 4.286036350731498E-5 0.0 20-21 0.0 0.0 0.0 4.286036350731498E-5 0.0 22-23 0.0 0.0 0.0 4.286036350731498E-5 0.0 24-25 0.0 0.0 0.0 4.286036350731498E-5 0.0 26-27 0.0 0.0 0.0 4.286036350731498E-5 0.0 28-29 0.0 0.0 0.0 4.286036350731498E-5 0.0 30-31 0.0 0.0 0.0 4.286036350731498E-5 0.0 32-33 0.0 0.0 0.0 4.286036350731498E-5 0.0 34-35 0.0 0.0 0.0 4.286036350731498E-5 0.0 36-37 0.0 0.0 0.0 4.286036350731498E-5 0.0 38-39 0.0 0.0 0.0 4.286036350731498E-5 0.0 40-41 0.0 0.0 0.0 4.286036350731498E-5 0.0 42-43 4.286036350731498E-5 0.0 0.0 4.286036350731498E-5 0.0 44-45 6.429054526097246E-5 2.143018175365749E-5 0.0 4.286036350731498E-5 0.0 46-47 8.572072701462996E-5 4.286036350731498E-5 0.0 4.286036350731498E-5 0.0 48-49 8.572072701462996E-5 4.286036350731498E-5 0.0 4.286036350731498E-5 0.0 50-51 8.572072701462996E-5 4.286036350731498E-5 0.0 4.286036350731498E-5 0.0 52-53 8.572072701462996E-5 4.286036350731498E-5 0.0 4.286036350731498E-5 0.0 54-55 1.0715090876828744E-4 4.286036350731498E-5 0.0 4.286036350731498E-5 0.0 56-57 1.5001127227560243E-4 4.286036350731498E-5 1.2858109052194493E-4 4.286036350731498E-5 0.0 58-59 3.2145272630486235E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 60-61 4.286036350731498E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 62-63 5.143243620877797E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 64-65 5.143243620877797E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 66-67 5.786149073487522E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 68-69 6.643356343633822E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 70-71 7.929167248853271E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 72-73 8.572072701462996E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 74-75 9.000676336536146E-4 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 76-77 0.0010286487241755594 4.286036350731498E-5 2.5716218104388986E-4 4.286036350731498E-5 0.0 78-79 0.0011572298146975044 4.286036350731498E-5 2.5716218104388986E-4 6.429054526097246E-5 0.0 80-81 0.0013929618139877369 4.286036350731498E-5 2.5716218104388986E-4 8.572072701462996E-5 0.0 82-83 0.0015644032680169966 4.286036350731498E-5 2.5716218104388986E-4 8.572072701462996E-5 0.0 84-85 0.0015858334497706541 4.286036350731498E-5 2.5716218104388986E-4 8.572072701462996E-5 0.0 86-87 0.0015858334497706541 1.2858109052194493E-4 2.5716218104388986E-4 8.572072701462996E-5 0.0 88 0.0015858334497706541 1.2858109052194493E-4 2.5716218104388986E-4 8.572072701462996E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 9345 0.0 79.86248 8 CACCGGC 9190 0.0 77.32774 9 CTTCACC 10555 0.0 73.73119 6 GTGTGGC 3650 0.0 72.27173 7 CCTTCAC 11470 0.0 71.57336 5 CGTAGTG 1995 0.0 71.52464 4 GGCGCGC 425 0.0 68.47433 6 TTCACCG 11565 0.0 66.96738 7 ATGCTGC 50 6.1190804E-9 65.72031 2 GCCTTCA 3295 0.0 64.246 4 GGGCCCG 620 0.0 63.593475 8 TGTGGCA 4105 0.0 62.20291 8 ACCTTCA 4485 0.0 61.328197 4 CGGGGCA 1080 0.0 60.84561 94 GCGTAGT 1390 0.0 59.770027 3 CGGGCCC 670 0.0 59.548267 7 AACGGGC 650 0.0 59.21415 5 CAGCGTA 970 0.0 58.957306 1 GCAGCCT 45 7.966824E-6 58.835995 1 TGTGTGG 4235 0.0 58.74183 6 >>END_MODULE