FastQCFastQC Report
Tue 7 Mar 2017
1HL_17A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_17A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3653761
Sequences flagged as poor quality0
Sequence length92
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA184540.5050686128621986No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG109220.2989248612593982No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG84160.23033799966664487No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA73140.2001772967635267No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT72440.19826146264082406No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC68920.18862755390951952No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG66580.18222319412791368No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG66440.18184002730337315No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT61700.16886709338678693No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT55340.15146037192908895No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA53640.14680763191681118No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC51740.14160751072661842No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA49880.1365168657720086No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA48950.13397154329470373No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA48860.13372522176464197No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA43970.12034175196461948No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA41520.11363633253516035No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC38770.10610984133882868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA258.359839E-786.03
TGCTGCA650.086.02
GCAGCCT402.1827873E-1186.03
GCTGCAG450.085.999993
GCAGCTA302.4600013E-885.999993
CAGCAGC4700.085.0851061
CAGCTGC4600.084.130431
AGCTGCA1550.083.22582
AGCAGCT1000.081.72
AGCAGCG1000.081.72
GCTGCAT950.081.473693
AGCAGCC1500.080.266662
CAGCCCT209750.080.198331
CAGCGTA89100.079.919191
CTGCTGC1650.078.1818161
GCAGCAA550.078.1818163
CAGCGTG63500.077.80631
GCGTAGT51000.077.4843143
AGCCCTT184700.077.339472
AGCTGCT1350.076.444442
Produced by FastQC (version 0.11.5)