##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_17A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3653761 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.824934088463914 12.0 12.0 12.0 2.0 32.0 2 14.531168568496954 12.0 12.0 12.0 12.0 22.0 3 13.717433077861415 12.0 12.0 12.0 12.0 22.0 4 13.724848724369219 12.0 12.0 12.0 12.0 22.0 5 22.424399680219917 22.0 22.0 22.0 22.0 27.0 6 26.758809621100013 27.0 22.0 32.0 22.0 32.0 7 34.91579826923545 37.0 37.0 37.0 27.0 41.0 8 37.548151616922944 41.0 37.0 41.0 27.0 41.0 9 38.49079646971983 41.0 37.0 41.0 32.0 41.0 10-11 38.85927308874335 41.0 41.0 41.0 34.5 41.0 12-13 39.03854795100172 41.0 41.0 41.0 37.0 41.0 14-15 38.99304073254929 41.0 41.0 41.0 37.0 41.0 16-17 38.89514763007213 41.0 41.0 41.0 37.0 41.0 18-19 38.83261630960536 41.0 41.0 41.0 37.0 41.0 20-21 38.84958882094368 41.0 41.0 41.0 37.0 41.0 22-23 38.833318189120746 41.0 41.0 41.0 34.5 41.0 24-25 38.8608755471417 41.0 41.0 41.0 37.0 41.0 26-27 38.829746390089554 41.0 41.0 41.0 37.0 41.0 28-29 38.774045702496686 41.0 41.0 41.0 34.5 41.0 30-31 38.65097101315604 41.0 41.0 41.0 32.0 41.0 32-33 38.69481638235232 41.0 41.0 41.0 32.0 41.0 34-35 38.666701242911074 41.0 41.0 41.0 32.0 41.0 36-37 38.58863647622272 41.0 41.0 41.0 32.0 41.0 38-39 38.49572659514402 41.0 41.0 41.0 32.0 41.0 40-41 38.54049238579097 41.0 41.0 41.0 32.0 41.0 42-43 38.27908858844353 41.0 41.0 41.0 32.0 41.0 44-45 38.39213279138947 41.0 41.0 41.0 32.0 41.0 46-47 38.41563049690443 41.0 41.0 41.0 32.0 41.0 48-49 38.34647381150546 41.0 41.0 41.0 32.0 41.0 50-51 38.2383523443378 41.0 41.0 41.0 32.0 41.0 52-53 38.19847576237198 41.0 41.0 41.0 32.0 41.0 54-55 38.06752343680935 41.0 37.0 41.0 32.0 41.0 56-57 37.86221211513288 41.0 37.0 41.0 27.0 41.0 58-59 37.86293575305008 41.0 37.0 41.0 29.5 41.0 60-61 37.85839823677575 41.0 37.0 41.0 29.5 41.0 62-63 37.859829775401295 41.0 37.0 41.0 29.5 41.0 64-65 37.85840247897988 41.0 37.0 41.0 29.5 41.0 66-67 37.705165718283155 41.0 37.0 41.0 27.0 41.0 68-69 37.73826558442109 41.0 37.0 41.0 27.0 41.0 70-71 37.716130447503275 41.0 37.0 41.0 27.0 41.0 72-73 37.56741040259612 41.0 37.0 41.0 27.0 41.0 74-75 37.503208474774354 41.0 37.0 41.0 27.0 41.0 76-77 36.432591923773884 39.0 34.5 41.0 24.5 41.0 78-79 36.96414995397893 41.0 37.0 41.0 27.0 41.0 80-81 37.60640077443489 41.0 37.0 41.0 27.0 41.0 82-83 37.79637830717444 41.0 37.0 41.0 27.0 41.0 84-85 37.707710082843406 41.0 37.0 41.0 27.0 41.0 86-87 37.58248870136826 41.0 37.0 41.0 27.0 41.0 88-89 37.503124588608834 41.0 37.0 41.0 27.0 41.0 90-91 37.34271932400614 41.0 37.0 41.0 27.0 41.0 92-93 37.22328170890214 41.0 37.0 41.0 27.0 41.0 94-95 37.056382998231136 41.0 37.0 41.0 27.0 41.0 96-97 37.01096158177834 41.0 37.0 41.0 27.0 41.0 98-99 36.89033067570648 41.0 37.0 41.0 27.0 41.0 100 36.761035546660004 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3746003897564787 1101 2 -0.5022116192443082 1101 3 0.01653794034149314 1101 4 -0.010184214035062311 1101 5 -1.1583649770906845 1101 6 -0.7634470026329083 1101 7 -0.3672609897821886 1101 8 -0.3369419887040692 1101 9 -0.1870853838871156 1101 10-11 -0.18497026087730717 1101 12-13 -0.09874652723132726 1101 14-15 -0.06703254622458843 1101 16-17 -0.025457031173424127 1101 18-19 -0.0753851399291321 1101 20-21 -0.012668849379160463 1101 22-23 -0.07474904784941572 1101 24-25 -0.141820374144487 1101 26-27 -0.023130826703564367 1101 28-29 -0.03784662218826185 1101 30-31 -0.09411930390920276 1101 32-33 -0.16519868857469078 1101 34-35 -0.1211228306866019 1101 36-37 -0.05686937374047574 1101 38-39 -0.09196976709394278 1101 40-41 -0.17162067276139226 1101 42-43 7.200943732073029E-4 1101 44-45 -0.07496547246788765 1101 46-47 -0.08823822902181888 1101 48-49 0.043673193659046206 1101 50-51 0.06582867508475232 1101 52-53 -0.03934599741214129 1101 54-55 -0.04016501485146762 1101 56-57 0.05836990999123515 1101 58-59 -0.07008129541725339 1101 60-61 -0.21333260286782973 1101 62-63 -0.1696028030853043 1101 64-65 -0.017894975126580448 1101 66-67 -0.03655005093132502 1101 68-69 0.019054114326053195 1101 70-71 -0.2139018888749291 1101 72-73 0.001881667388289543 1101 74-75 0.1022038509890777 1101 76-77 -0.30670046400588546 1101 78-79 -0.06372948120998245 1101 80-81 -0.06649483335765183 1101 82-83 -0.036766041937561056 1101 84-85 -0.14752240911867887 1101 86-87 -0.13766266862580778 1101 88-89 -0.41082672825006483 1101 90-91 -0.26635677516733125 1101 92-93 -0.00790092072153925 1101 94-95 -0.07009381770633638 1101 96-97 -0.2143029824681406 1101 98-99 -0.3459281988723859 1101 100 -0.6051353990937542 1102 1 1.5476985209225873 1102 2 0.8074303996740717 1102 3 -0.15174442606856253 1102 4 0.05030660611040716 1102 5 -0.7486199174290817 1102 6 1.320974257126295E-4 1102 7 0.030548119073010582 1102 8 -0.17388369053286112 1102 9 -0.24489328521868003 1102 10-11 -0.3634589724419399 1102 12-13 -0.35848266421037067 1102 14-15 -0.3340564800308883 1102 16-17 -0.30230202984743926 1102 18-19 -0.26155901339747345 1102 20-21 -0.15293294167676663 1102 22-23 -0.13462086883327373 1102 24-25 -0.125261155427566 1102 26-27 -0.21093762750726341 1102 28-29 -0.2889941517706802 1102 30-31 -0.3044965270328248 1102 32-33 -0.3466892523124585 1102 34-35 -0.08104212148809609 1102 36-37 -0.20379834689447307 1102 38-39 -0.23037480797013643 1102 40-41 -0.1243365691986611 1102 42-43 -0.25615916014143636 1102 44-45 -0.055211809200372386 1102 46-47 -0.4657953322796118 1102 48-49 -0.3949057439952881 1102 50-51 -0.40552225344742254 1102 52-53 -0.34338110386910614 1102 54-55 -0.35948604265279016 1102 56-57 -0.20462969336018233 1102 58-59 -0.30712773845767316 1102 60-61 -0.3321285680575272 1102 62-63 -0.4266643325286239 1102 64-65 -0.38863974398897483 1102 66-67 -0.2957805373875786 1102 68-69 -0.5280353906886006 1102 70-71 -0.5600983422710115 1102 72-73 -0.2629310842905923 1102 74-75 -0.4325598373995021 1102 76-77 -0.447787134014888 1102 78-79 -0.4473562536139326 1102 80-81 -0.5175064401054144 1102 82-83 -0.45212116661438273 1102 84-85 -0.42686083901696037 1102 86-87 -0.44149500855440493 1102 88-89 -0.5331340174129977 1102 90-91 -0.5373759021888844 1102 92-93 -0.5138372272888603 1102 94-95 -0.28666791074527254 1102 96-97 -0.20236688836384076 1102 98-99 -0.4623645730763215 1102 100 -0.8263710205824779 1103 1 1.6725828011714725 1103 2 0.6333107804940123 1103 3 0.5205774288685632 1103 4 0.9694758155098988 1103 5 2.762237149177185 1103 6 -0.1651327335106032 1103 7 -0.5249181691600455 1103 8 -0.37505292527389145 1103 9 -0.14411672550649968 1103 10-11 -0.22356527622836353 1103 12-13 -0.3684316920852666 1103 14-15 -0.4114354266782456 1103 16-17 -0.38725176055401533 1103 18-19 -0.36722104520268317 1103 20-21 -0.3602670001476085 1103 22-23 -0.1302029777876328 1103 24-25 -0.2646882110023441 1103 26-27 -0.2712194016365572 1103 28-29 -0.2582119060512227 1103 30-31 -0.11017331830329624 1103 32-33 -0.41506027260231804 1103 34-35 -0.46239136429090166 1103 36-37 -0.3195166131532474 1103 38-39 -0.11321682566328661 1103 40-41 -0.3074394661322586 1103 42-43 -0.12075180681943465 1103 44-45 -0.5249487185148922 1103 46-47 -0.5247500414102504 1103 48-49 -0.21793990399066132 1103 50-51 -0.22361496229137856 1103 52-53 -0.14479527050079355 1103 54-55 -0.20931269070398173 1103 56-57 -0.2560941965987453 1103 58-59 -0.47853551625041035 1103 60-61 -0.1284116179748125 1103 62-63 -0.11657578761589349 1103 64-65 -0.2372795469110116 1103 66-67 -0.4378012398391249 1103 68-69 -0.3426722464691281 1103 70-71 -0.47720162249535036 1103 72-73 -0.4210416331803586 1103 74-75 -0.4721516327602657 1103 76-77 -0.3991776617444742 1103 78-79 -0.3135629878897106 1103 80-81 -0.1512828279703058 1103 82-83 -0.08722565887689626 1103 84-85 -0.33108091759156366 1103 86-87 -0.30584958337489354 1103 88-89 -0.2443654755254201 1103 90-91 -0.6198222157476394 1103 92-93 -0.22977498491131598 1103 94-95 -0.20777530223796248 1103 96-97 -0.31583782558097084 1103 98-99 -0.2293554299291074 1103 100 -0.35929413119274045 1104 1 1.735239754073156 1104 2 -0.43834116233559506 1104 3 0.038921694278860386 1104 4 0.06291751800529255 1104 5 -1.1974142005847597 1104 6 -0.4410347728946391 1104 7 -0.34022766705452767 1104 8 -0.24540319021297563 1104 9 -0.2706039546492036 1104 10-11 -0.14706096607197594 1104 12-13 -0.12710689590092983 1104 14-15 -0.2511090614661242 1104 16-17 -0.032761116577795235 1104 18-19 -0.12481500569268178 1104 20-21 -0.09970569642371174 1104 22-23 -0.031154125697270274 1104 24-25 -0.07733594612252404 1104 26-27 -0.0698046653330806 1104 28-29 -0.12909112983675186 1104 30-31 -0.06181111513909343 1104 32-33 -0.24372738726457044 1104 34-35 -0.2118988609145518 1104 36-37 -0.26876326367550973 1104 38-39 -0.30373477500666013 1104 40-41 -0.19141517696013466 1104 42-43 -0.15342554146253917 1104 44-45 -0.2213920142928103 1104 46-47 -0.3249728427688652 1104 48-49 -0.4337178510083177 1104 50-51 -0.3327182379241762 1104 52-53 -0.3346900293533608 1104 54-55 -0.37386666792976087 1104 56-57 -0.21630457913618528 1104 58-59 -0.2746792170141248 1104 60-61 -0.1388193979584429 1104 62-63 -0.2403895667175604 1104 64-65 -0.2433567300117545 1104 66-67 -0.5880649172909145 1104 68-69 -0.5243090467087015 1104 70-71 -0.4506550963666811 1104 72-73 -0.19968514347586108 1104 74-75 -0.39176177097026255 1104 76-77 -0.3358025531971549 1104 78-79 -0.24743917112766667 1104 80-81 -0.39840974083112 1104 82-83 -0.3236201889230941 1104 84-85 -0.2766143425387213 1104 86-87 -0.28177391169691646 1104 88-89 -0.2663425248631839 1104 90-91 -0.3385205613294531 1104 92-93 -0.4707516570291048 1104 94-95 -0.26352728102914114 1104 96-97 -0.35805438466773865 1104 98-99 -0.412890408549238 1104 100 -0.2468330891560413 1105 1 1.7079774725582766 1105 2 0.4169272855382289 1105 3 -0.1078616760470652 1105 4 -0.14391228017142588 1105 5 -1.1971752532275168 1105 6 0.2996319622955568 1105 7 -0.01035562526073619 1105 8 0.06023314177061678 1105 9 0.035363584921242364 1105 10-11 -0.1255707803605688 1105 12-13 -0.26208342216769154 1105 14-15 -0.1037079363654243 1105 16-17 -0.16926178563039684 1105 18-19 -0.15535707673665655 1105 20-21 0.03306834205970688 1105 22-23 -0.04694750298680361 1105 24-25 -0.15269795136874365 1105 26-27 -0.12499638455552997 1105 28-29 -0.18424563206010447 1105 30-31 -0.1509702442176888 1105 32-33 -0.19445208046374773 1105 34-35 -0.037624265266586576 1105 36-37 -0.1941998830786389 1105 38-39 -0.140307488583872 1105 40-41 -0.17443185131357808 1105 42-43 0.09453430324443701 1105 44-45 -0.01978160392538797 1105 46-47 -0.22355533498221547 1105 48-49 -0.20216714713767914 1105 50-51 -0.22262243710158458 1105 52-53 -0.041851983320725594 1105 54-55 -0.1045303745570152 1105 56-57 0.027340796056670058 1105 58-59 0.05118675855104016 1105 60-61 -0.16305809461059795 1105 62-63 -0.2310380963948191 1105 64-65 -0.2915752162688676 1105 66-67 -0.20201786893554896 1105 68-69 -0.28915483653211993 1105 70-71 -0.23460818834548292 1105 72-73 -0.19748739671150872 1105 74-75 -0.2175332106167147 1105 76-77 -0.30196518949196616 1105 78-79 -0.23780930816966617 1105 80-81 -0.45066231273909807 1105 82-83 -0.20399840086864174 1105 84-85 -0.2347188110818763 1105 86-87 -0.3132695438909394 1105 88-89 -0.09846906341866912 1105 90-91 -0.1510211302517348 1105 92-93 -0.08459513854520395 1105 94-95 -0.019315970399091498 1105 96-97 -0.1804535375045333 1105 98-99 -0.1470249203608205 1105 100 -0.1632458900590379 1106 1 1.191667715763984 1106 2 -0.5461633688349465 1106 3 -0.12185500711132846 1106 4 -0.19034715738338903 1106 5 -1.1785617962299213 1106 6 -0.658933532761619 1106 7 -0.15087601953174357 1106 8 -0.10830696301174925 1106 9 -0.1012371571858921 1106 10-11 -0.1990352461315652 1106 12-13 -0.10674329732837151 1106 14-15 -0.03790556721423144 1106 16-17 -0.09143759239080396 1106 18-19 -0.19575787004979617 1106 20-21 -0.2012634956127144 1106 22-23 -0.13510710312416307 1106 24-25 -0.23792232329753205 1106 26-27 -0.14173140186224487 1106 28-29 -0.24161145182713994 1106 30-31 -0.059514922592356356 1106 32-33 -0.16637728931433315 1106 34-35 -0.14734594090316477 1106 36-37 -0.363892597695596 1106 38-39 -0.15477090440394647 1106 40-41 -0.14215830111194094 1106 42-43 -0.1465988536282623 1106 44-45 -0.18843490891561743 1106 46-47 -0.3892912654879197 1106 48-49 -0.3624295816866834 1106 50-51 -0.38159418035440495 1106 52-53 -0.12916562083086092 1106 54-55 -0.3328492109196617 1106 56-57 -0.23814322599989168 1106 58-59 -0.41411889720485107 1106 60-61 -0.14618015254479388 1106 62-63 -0.3062032243490407 1106 64-65 -0.4958033010125149 1106 66-67 -0.3286275530993734 1106 68-69 -0.44485327041864764 1106 70-71 -0.5603410267381292 1106 72-73 -0.39607360968592786 1106 74-75 -0.44866239408613495 1106 76-77 -0.46967633067161785 1106 78-79 -0.2958318220955718 1106 80-81 -0.35443017026182133 1106 82-83 -0.34788232279240816 1106 84-85 -0.4292619152898851 1106 86-87 -0.5145039959563462 1106 88-89 -0.5762638739868606 1106 90-91 -0.6011642259507326 1106 92-93 -0.4328682085956146 1106 94-95 -0.3238269986824349 1106 96-97 -0.42228564868081264 1106 98-99 -0.5215133351932408 1106 100 -0.5329083862983595 1107 1 1.432826623933929 1107 2 -0.6674081200590347 1107 3 -0.18975957223309514 1107 4 -0.1575720129999585 1107 5 -1.1995415061584467 1107 6 -0.36059428375172686 1107 7 -0.3821490836469579 1107 8 -0.3642363335665735 1107 9 -0.3835438515663796 1107 10-11 -0.24462960325729455 1107 12-13 -0.3432472421603734 1107 14-15 -0.39883614425149716 1107 16-17 -0.4059610241271798 1107 18-19 -0.3140768532333169 1107 20-21 -0.3454178476277292 1107 22-23 -0.25458754213754275 1107 24-25 -0.22087695840297528 1107 26-27 -0.19261475098055314 1107 28-29 -0.1941832221390527 1107 30-31 -0.36385637367324364 1107 32-33 -0.205016862015583 1107 34-35 -0.41442517197168627 1107 36-37 -0.267190046995438 1107 38-39 -0.44200204835327384 1107 40-41 -0.5415822105537913 1107 42-43 -0.3495629712624151 1107 44-45 -0.17852087511948156 1107 46-47 -0.3753679385057822 1107 48-49 -0.47041283948177437 1107 50-51 -0.3727174905573918 1107 52-53 -0.3358306762886514 1107 54-55 -0.48146976083705795 1107 56-57 -0.49789139251217307 1107 58-59 -0.6439618851558038 1107 60-61 -0.3757754940986331 1107 62-63 -0.5519356053758244 1107 64-65 -0.6464573607393618 1107 66-67 -0.5288363880914417 1107 68-69 -0.6493919727207356 1107 70-71 -0.44208823653099927 1107 72-73 -0.41184033409184906 1107 74-75 -0.49583816684103255 1107 76-77 -0.4905293310799408 1107 78-79 -0.7990266915241548 1107 80-81 -0.5261793927829856 1107 82-83 -0.5587400490713677 1107 84-85 -0.3264097676933986 1107 86-87 -0.34393537519861894 1107 88-89 -0.24524867384739224 1107 90-91 -0.3526890696756553 1107 92-93 -0.3931444372738824 1107 94-95 -0.38459147753012246 1107 96-97 -0.562280714995012 1107 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0.23331755795226883 2223 20-21 0.2381644426435514 2223 22-23 0.43834248720837365 2223 24-25 0.4461794928015337 2223 26-27 0.24295732542910287 2223 28-29 0.3137218483672797 2223 30-31 0.33535687988883467 2223 32-33 0.3227637500283578 2223 34-35 0.3423606239033887 2223 36-37 0.22864111218482464 2223 38-39 0.2062274251722016 2223 40-41 0.43419681771889174 2223 42-43 0.5562895737064508 2223 44-45 0.4973747977938672 2223 46-47 0.49280261062620667 2223 48-49 0.4086027165130943 2223 50-51 0.4421469829624556 2223 52-53 0.40656069452126786 2223 54-55 0.5571430100911812 2223 56-57 0.5425511368853151 2223 58-59 0.5195488053001895 2223 60-61 0.4828114945940243 2223 62-63 0.5219413031116815 2223 64-65 0.38879064651102624 2223 66-67 0.48832841752520295 2223 68-69 0.5558740854706627 2223 70-71 0.612456215411747 2223 72-73 0.6379348919294117 2223 74-75 0.5864868033647852 2223 76-77 0.8051766494008916 2223 78-79 0.7839636539232941 2223 80-81 0.5182793959658625 2223 82-83 0.48620958872817255 2223 84-85 0.5060249261252068 2223 86-87 0.5027939608441017 2223 88-89 0.5424329468660218 2223 90-91 0.6492587589034216 2223 92-93 0.6035638543426032 2223 94-95 0.7099148768737749 2223 96-97 0.7393468706841233 2223 98-99 0.8644569592324203 2223 100 0.9488393674582412 2224 1 1.2875931475491953 2224 2 -0.8049111945427025 2224 3 -0.005665002446022527 2224 4 -0.152625278073101 2224 5 -0.9316727211297646 2224 6 0.6857155185195332 2224 7 0.4452244082403993 2224 8 0.29443173358205144 2224 9 0.3651029004293278 2224 10-11 0.4024528400438996 2224 12-13 0.2844922796001299 2224 14-15 0.27145641734175996 2224 16-17 0.22961059033884368 2224 18-19 0.29016895644027585 2224 20-21 0.1940946371519061 2224 22-23 0.36299848543188773 2224 24-25 0.4244456637523939 2224 26-27 0.3698069613557422 2224 28-29 0.2778364104587254 2224 30-31 0.2794107636983014 2224 32-33 0.3277585594500323 2224 34-35 0.3616952991285558 2224 36-37 0.30325535492653444 2224 38-39 0.2216808010268636 2224 40-41 0.28654474366786076 2224 42-43 0.3756954659380938 2224 44-45 0.3680569346924827 2224 46-47 0.3354909857461621 2224 48-49 0.22447350061105453 2224 50-51 0.40941784906898704 2224 52-53 0.35553820738961406 2224 54-55 0.3033575339632506 2224 56-57 0.48071134162092477 2224 58-59 0.41924037538325365 2224 60-61 0.3177756552355504 2224 62-63 0.3476893931510787 2224 64-65 0.3501906442391203 2224 66-67 0.4339730038051428 2224 68-69 0.4974248389931333 2224 70-71 0.5244248865247627 2224 72-73 0.30239890654584656 2224 74-75 0.4165266936829042 2224 76-77 0.5207274608203818 2224 78-79 0.3419035531458263 2224 80-81 0.32627874957121605 2224 82-83 0.3386603918295492 2224 84-85 0.24675068366931896 2224 86-87 0.44448341072342146 2224 88-89 0.311766889217445 2224 90-91 0.5022580263961132 2224 92-93 0.2986794889947717 2224 94-95 0.577253277296208 2224 96-97 0.493762893063149 2224 98-99 0.4460641000610721 2224 100 0.70904527566654 2225 1 1.4534661062367231 2225 2 1.4714388211806462 2225 3 0.8331282151210093 2225 4 0.8965983012538494 2225 5 3.2282437971006637 2225 6 -0.06467533099376865 2225 7 0.331643455891367 2225 8 0.46812475459324787 2225 9 0.24706997802559272 2225 10-11 0.24258254231683196 2225 12-13 0.24777923603443952 2225 14-15 0.22085250666825829 2225 16-17 0.12742571502807465 2225 18-19 0.2188401071939623 2225 20-21 0.2568485357326722 2225 22-23 0.2605601653670533 2225 24-25 0.287262092669728 2225 26-27 0.192850298034827 2225 28-29 0.2489451048845055 2225 30-31 0.2213205278704038 2225 32-33 0.23280824020739033 2225 34-35 0.23757900443220592 2225 36-37 0.32907613890446896 2225 38-39 0.2833741415320574 2225 40-41 0.286039110910572 2225 42-43 0.6271161529507197 2225 44-45 0.43354105466806203 2225 46-47 0.3785119410890587 2225 48-49 0.3528674959113616 2225 50-51 0.3598788252795302 2225 52-53 0.3759996472015814 2225 54-55 0.2920090738181784 2225 56-57 0.370882907660949 2225 58-59 0.5188526817109675 2225 60-61 0.42109216486421985 2225 62-63 0.3976223385114608 2225 64-65 0.49298463037911233 2225 66-67 0.36531153841131925 2225 68-69 0.5449860162279592 2225 70-71 0.4730324345144865 2225 72-73 0.5027576670208589 2225 74-75 0.4696050071344402 2225 76-77 0.47339319693286797 2225 78-79 0.4727632936072226 2225 80-81 0.3513228615259294 2225 82-83 0.34115853323468315 2225 84-85 0.33780479600943636 2225 86-87 0.47021412755734815 2225 88-89 0.3076196114235543 2225 90-91 0.42223469551483817 2225 92-93 0.4451193093408463 2225 94-95 0.4389343016430942 2225 96-97 0.5266191079195153 2225 98-99 0.43489717059383537 2225 100 0.47628691980943927 2226 1 1.5823554105877147 2226 2 1.2822417917714457 2226 3 0.8692665242931987 2226 4 0.8353888763673751 2226 5 3.062441328373378 2226 6 -0.3571635923510499 2226 7 -0.12040872467802188 2226 8 -0.012122779933307015 2226 9 0.04565891430657132 2226 10-11 -0.03722056725154488 2226 12-13 -0.0110086146771593 2226 14-15 0.008647792680825717 2226 16-17 -0.04878738835250118 2226 18-19 0.035849916143895655 2226 20-21 0.19579849387395143 2226 22-23 0.13946546317065156 2226 24-25 0.09723202497881545 2226 26-27 -0.024295703436571614 2226 28-29 0.030980955587281755 2226 30-31 -0.03634226617474212 2226 32-33 0.05653047364342001 2226 34-35 -0.04451640809408275 2226 36-37 0.12624372602191158 2226 38-39 0.027947121862318625 2226 40-41 0.029391625357071405 2226 42-43 0.21503574669496572 2226 44-45 0.003324348900164864 2226 46-47 0.019525661265404892 2226 48-49 -0.032573195402868294 2226 50-51 0.02047652817853418 2226 52-53 -0.005230354088922695 2226 54-55 0.06113021153724674 2226 56-57 0.10446369831106495 2226 58-59 0.24796730071935258 2226 60-61 0.26355693239111133 2226 62-63 0.18658556018844052 2226 64-65 0.1965498467153708 2226 66-67 0.23002250914770883 2226 68-69 0.16917090164314175 2226 70-71 0.2393170782630918 2226 72-73 0.26516072739559604 2226 74-75 0.17408240176791878 2226 76-77 0.29809916557908167 2226 78-79 0.22846040960166647 2226 80-81 0.15388892827267853 2226 82-83 0.11588965020638398 2226 84-85 0.07910311691750849 2226 86-87 0.1505571264510408 2226 88-89 0.1664135561921043 2226 90-91 0.07118392567826959 2226 92-93 0.08661917532673868 2226 94-95 0.18526090890235736 2226 96-97 0.2829689222300118 2226 98-99 0.28939452946816147 2226 100 0.38789138297541115 2227 1 1.2295641383107458 2227 2 1.4487993122046134 2227 3 0.6435247808468496 2227 4 -0.07144469016710175 2227 5 3.177646742321052 2227 6 0.15642078836279083 2227 7 0.4622613566276357 2227 8 0.43111860183960715 2227 9 0.4599608992955595 2227 10-11 0.4502479082608559 2227 12-13 0.2646390016464011 2227 14-15 0.41627998570290714 2227 16-17 0.36849559562084977 2227 18-19 0.3378909507103103 2227 20-21 0.13951170763601795 2227 22-23 0.27963462694853547 2227 24-25 0.3270484384372665 2227 26-27 0.3328323830735087 2227 28-29 0.1988011580581457 2227 30-31 0.30085186208920334 2227 32-33 0.1694008852222666 2227 34-35 0.20228453052671114 2227 36-37 0.27069862801300815 2227 38-39 0.24807317195767808 2227 40-41 0.3144155105286046 2227 42-43 0.6130836856349191 2227 44-45 0.3949351448430889 2227 46-47 0.4851799367976142 2227 48-49 0.3258988806468395 2227 50-51 0.36902711037154035 2227 52-53 0.31047222739435654 2227 54-55 0.4062477949770553 2227 56-57 0.49291044985779564 2227 58-59 0.5593921044862071 2227 60-61 0.502814034902805 2227 62-63 0.5201545334297606 2227 64-65 0.47368207268378626 2227 66-67 0.45732446395123105 2227 68-69 0.4332251357255501 2227 70-71 0.5753001986968442 2227 72-73 0.5277673474367575 2227 74-75 0.46757625552816506 2227 76-77 0.734672411904647 2227 78-79 0.5797592037658603 2227 80-81 0.43479314131728586 2227 82-83 0.45511780043725025 2227 84-85 0.6532379351549764 2227 86-87 0.5302322387906742 2227 88-89 0.5527354992969151 2227 90-91 0.5693899149904027 2227 92-93 0.3674863927677592 2227 94-95 0.6041274795645108 2227 96-97 0.44147028186446136 2227 98-99 0.7878389655686533 2227 100 0.7892045060338333 2228 1 1.4402334253130977 2228 2 1.430772431661497 2228 3 -0.10447908910738235 2228 4 0.02381318574510871 2228 5 3.083544132070724 2228 6 0.19989453105021227 2228 7 0.259938213963288 2228 8 0.3496848168568363 2228 9 0.2994569805861431 2228 10-11 0.15078535753457345 2228 12-13 0.1989475686470783 2228 14-15 0.1416250470257836 2228 16-17 0.2323736329990851 2228 18-19 0.045971151778537944 2228 20-21 0.1021017166682654 2228 22-23 0.21445795581124827 2228 24-25 0.22640956909688725 2228 26-27 0.13865098444797752 2228 28-29 0.14757380901951223 2228 30-31 0.3019788033116271 2228 32-33 0.18058082007791398 2228 34-35 0.09094348068740032 2228 36-37 0.32080790462911324 2228 38-39 0.15077519346827728 2228 40-41 0.16046902458857204 2228 42-43 0.3784682210110333 2228 44-45 0.3429792076412497 2228 46-47 0.23923209611358232 2228 48-49 0.2411771854449256 2228 50-51 0.20425339849739288 2228 52-53 0.09957978095586384 2228 54-55 0.22929095232182561 2228 56-57 0.2607532215444266 2228 58-59 0.34899909685235286 2228 60-61 0.388295818759282 2228 62-63 0.36147990917434214 2228 64-65 0.3649185468352698 2228 66-67 0.5013091609530917 2228 68-69 0.3805789644375466 2228 70-71 0.4567088352920621 2228 72-73 0.5313407308588438 2228 74-75 0.46419910010796883 2228 76-77 0.4357446823155584 2228 78-79 0.3403132149429666 2228 80-81 0.21857003537255082 2228 82-83 0.3111653840096693 2228 84-85 0.3195010946431154 2228 86-87 0.25829978951417587 2228 88-89 0.27840280916051796 2228 90-91 0.316431821973687 2228 92-93 0.3544181929038288 2228 94-95 0.5867525718975486 2228 96-97 0.46035330341536707 2228 98-99 0.5300245777563717 2228 100 0.5677046963183443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 27.0 13 41.0 14 195.0 15 699.0 16 2444.0 17 6192.0 18 10198.0 19 13063.0 20 15244.0 21 17801.0 22 20116.0 23 22782.0 24 25835.0 25 29340.0 26 33215.0 27 37404.0 28 42511.0 29 48129.0 30 54849.0 31 63548.0 32 73562.0 33 88121.0 34 107847.0 35 138439.0 36 195916.0 37 322889.0 38 794887.0 39 1486876.0 40 1591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.05487046832169 18.52314582448554 21.30849001969036 34.11349368750241 2 20.826045900562466 50.178812387951446 12.47961571622121 16.51552599526488 3 39.20743584487327 15.753931360042433 23.75793052692828 21.28070226815602 4 18.02019891284624 17.33063547396778 22.041479998281226 42.60768561490475 5 16.967502800538952 34.70073713086324 26.540405899564863 21.79135416903295 6 17.48398431096068 28.88327944821788 29.058249841738416 24.574486399083025 7 19.936005666489955 25.833463108287596 36.445158837701754 17.785372387520695 8 21.823786503824415 28.066230933003006 33.138784939682694 16.97119762348988 9 20.97720677406103 28.257485916566516 26.991174299577885 23.774133009794564 10-11 19.726550258760767 30.441632608153625 28.22009978211492 21.611717350970686 12-13 24.315274042281366 26.52762454906054 27.3157987071404 21.841302701517694 14-15 21.824443361237915 27.598986359534738 28.51186763447308 22.06470264475427 16-17 20.78908554774108 29.830016249010267 28.289576138121785 21.091322065126867 18-19 21.271437841719806 29.61380068373383 29.80281140446789 19.311950070078478 20-21 22.761190455533352 28.812503061913464 30.01945119015721 18.40685529239597 22-23 21.69910675602482 30.614673483022013 28.629417742430334 19.05680201852283 24-25 22.26058299927116 28.312634570241457 26.726693398938796 22.700089031548586 26-27 20.04689140860609 26.58292373255941 30.137001298114463 23.233183560720036 28-29 19.47718933227798 28.14351607430282 30.64720338085436 21.732091212564843 30-31 20.030979459206158 30.11795761660716 28.316356171811456 21.534706752375225 32-33 20.592904133576333 30.084083222739526 28.74106981819555 20.581942825488586 34-35 21.39458492222124 29.117777544836677 30.053484614894078 19.434152918048007 36-37 21.728254803748793 28.327605445457433 28.75283851352072 21.191301237273045 38-39 21.533980343375127 29.16652297788835 29.072263139312426 20.227233539424095 40-41 20.994997274686803 29.959628689590318 29.701740026605844 19.34363400911704 42-43 20.43139990738298 29.95349169457402 29.45007822087737 20.165030177165637 44-45 20.40320918636988 30.237391006144083 28.852502941489604 20.506896865996435 46-47 19.76408446222925 28.374320086280125 28.901596622912155 22.95999882857847 48-49 20.776961054650265 28.026737381016435 30.306415772679163 20.889885791654134 50-51 21.699010964318685 30.89041675139671 28.1635553064363 19.247016977848304 52-53 20.395329786800207 30.53610728166912 28.239016845621805 20.829546085908866 54-55 22.444823702337818 28.63771972963344 27.979692532836687 20.937764035192057 56-57 21.433814205714764 28.692474842441495 29.408726378199805 20.464984573643928 58-59 21.286746234035807 27.971557651487476 29.099208382097462 21.64248773237925 60-61 19.51835283907357 30.663357558193237 29.46623246477903 20.352057137954162 62-63 20.51546511683607 31.162352419488943 27.454091195568942 20.86809126810605 64-65 19.563410732713432 30.40319211375586 28.728928010899473 21.30446914263123 66-67 20.24665816948618 30.5336063305728 29.298427018078083 19.921308481862933 68-69 20.825226390012922 29.981914525881688 29.401909429762924 19.79094965434247 70-71 19.51549376108618 31.140501527056642 29.060001461507746 20.284003250349432 72-73 20.036313267342884 30.84709153116474 28.632113594731567 20.484481606760816 74-75 20.01898865306187 29.430058506837202 30.195406869797996 20.35554597030293 76-77 19.418319917476815 30.817478209439535 28.58897995791186 21.17522191517179 78-79 19.40217217272832 29.679595901319217 28.555028640351683 22.363203285600783 80-81 20.994618410166204 29.4734756290313 28.18651123926549 21.345394721537005 82-83 20.37885072395266 28.26692824188555 29.83367001837285 21.520551015788936 84-85 20.561717090964628 28.927220472275007 28.62731032489536 21.883752111865007 86-87 20.96114113648922 28.16633326591422 29.6185081618639 21.25401743573266 88-89 20.58777243503338 27.295655079793125 30.362221283767603 21.754351201405893 90-91 20.585363957850554 27.6140393419274 29.85033777524036 21.950258924981682 92-93 20.450352390317814 28.03028167414344 30.291458581992636 21.22790735354611 94-95 20.956488396476946 28.10287810286442 29.71572305906161 21.22491044159703 96-97 19.99375438075999 28.621809144057313 29.92023835166011 21.464198123522586 98-99 21.817956894279618 28.756697550825027 28.73603391135873 20.689311643536616 100 20.453472463031925 30.57307251350047 28.053394844380904 20.9200601790867 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 12.0 2 16.0 3 19.0 4 27.5 5 33.5 6 37.5 7 54.0 8 82.0 9 95.5 10 108.5 11 131.5 12 145.0 13 153.5 14 191.0 15 250.0 16 316.0 17 412.5 18 596.5 19 962.0 20 1499.0 21 2334.0 22 3648.5 23 5723.0 24 8761.5 25 13221.5 26 19257.5 27 25947.5 28 33525.5 29 42382.5 30 52242.5 31 63493.0 32 76532.0 33 91079.0 34 107206.0 35 125129.0 36 142876.5 37 156312.5 38 165692.5 39 173499.5 40 179622.0 41 181668.5 42 178922.5 43 175411.5 44 173236.5 45 167334.5 46 154826.0 47 141929.0 48 133334.5 49 128502.0 50 123793.0 51 114577.0 52 100203.0 53 83702.5 54 68696.5 55 56102.0 56 44092.5 57 32735.5 58 22991.0 59 15980.5 60 12091.5 61 9901.0 62 8159.5 63 6325.0 64 4568.5 65 3335.5 66 2940.0 67 2781.0 68 2288.5 69 1957.0 70 2102.5 71 2033.0 72 1298.5 73 601.0 74 279.0 75 165.0 76 132.0 77 102.5 78 86.0 79 69.5 80 62.0 81 51.5 82 39.5 83 35.5 84 27.5 85 25.0 86 33.0 87 27.5 88 18.5 89 19.5 90 18.0 91 20.5 92 23.0 93 22.0 94 20.0 95 20.0 96 24.0 97 28.0 98 54.5 99 129.5 100 133.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.389250692642458 2 0.005774871427003572 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.736905889575153E-4 30-31 0.010194974438667445 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.484408257682974E-4 40-41 0.0014368755920269552 42-43 1.3684529447875764E-4 44-45 0.0 46-47 0.002203209241107998 48-49 0.0 50-51 0.0 52-53 0.0014231910625790796 54-55 0.019719406934388978 56-57 0.01191922514909979 58-59 0.01495719068652821 60-61 2.0526794171813647E-4 62-63 0.012548713503702076 64-65 1.7789888282238495E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1895247116601223E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3653761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.25579014429714 #Duplication Level Percentage of deduplicated Percentage of total 1 85.07490808252275 61.47154704956134 2 9.133507255324924 13.198975670443462 3 2.5844700161261285 5.602287693583133 4 1.1226585482326457 3.244743218591973 5 0.5953571187689818 2.150899951734247 6 0.3582301830046901 1.5530522955924027 7 0.24532126606089838 1.240811734290069 8 0.1739684191550406 1.0056180468961389 9 0.12018173476314778 0.7815443585601227 >10 0.5534107315077136 6.7653727763656715 >50 0.026775052311791343 1.3090009276155055 >100 0.010558598560526143 1.3330734642133835 >500 5.37759487595441E-4 0.24854114188433835 >1k 1.1523417311866512E-4 0.09453167066816309 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.7369058895751528E-5 0.0 0.0 0.0 0.0 14-15 2.7369058895751528E-5 0.0 0.0 2.7369058895751528E-5 0.0 16-17 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 18-19 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 20-21 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 22-23 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 24-25 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 26-27 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 28-29 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 30-31 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 32-33 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 34-35 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 36-37 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 38-39 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 40-41 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 42-43 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 44-45 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 46-47 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 48-49 2.7369058895751528E-5 0.0 0.0 5.4738117791503056E-5 0.0 50-51 4.105358834362729E-5 0.0 0.0 8.210717668725458E-5 0.0 52-53 1.0947623558300611E-4 0.0 0.0 8.210717668725458E-5 0.0 54-55 1.0947623558300611E-4 0.0 0.0 8.210717668725458E-5 0.0 56-57 1.0947623558300611E-4 0.0 1.7789888282238492E-4 8.210717668725458E-5 0.0 58-59 1.3684529447875764E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 60-61 1.915834122702607E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 62-63 2.4632153006176377E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 64-65 3.421132361968941E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 66-67 3.694822950926456E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 68-69 4.926430601235275E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 70-71 5.473811779150306E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 72-73 6.021192957065336E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 74-75 7.526491196331671E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 76-77 9.305480024555519E-4 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 78-79 0.0011221314147258127 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 80-81 0.0013137148269960733 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 82-83 0.0016968816515365948 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 84-85 0.0018063578871196008 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 86-87 0.0018337269460153524 0.0 3.5579776564476984E-4 8.210717668725458E-5 0.0 88 0.0018337269460153524 2.7369058895751528E-5 3.5579776564476984E-4 8.210717668725458E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 14780 0.0 81.39494 8 CACCGGC 14305 0.0 81.01333 9 CTTCACC 16785 0.0 74.832115 6 CCTTCAC 18200 0.0 72.72789 5 TTCACCG 18055 0.0 69.15243 7 CGGGCCC 1190 0.0 69.02626 7 CGTAGTG 3195 0.0 68.166405 4 GTGTGGC 5745 0.0 67.97615 7 GGGCCCG 1225 0.0 67.43725 8 AACGGGC 1210 0.0 65.55784 5 ACCTTCA 7490 0.0 63.106037 4 CGGGGCA 1395 0.0 62.92028 94 GCGTAGT 1995 0.0 62.583813 3 ACGGGCC 1305 0.0 62.224133 6 GCAGCCA 220 0.0 61.87602 2 GGCCCGA 1355 0.0 60.62085 9 GCTGCCA 225 0.0 60.500996 2 TGTGGCA 6850 0.0 58.723713 8 GCCTTCA 5625 0.0 58.161224 4 TGTGTGG 6400 0.0 56.69213 6 >>END_MODULE