FastQCFastQC Report
Tue 7 Mar 2017
1HL_17A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_17A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3653761
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG147800.081.394948
CACCGGC143050.081.013339
CTTCACC167850.074.8321156
CCTTCAC182000.072.727895
TTCACCG180550.069.152437
CGGGCCC11900.069.026267
CGTAGTG31950.068.1664054
GTGTGGC57450.067.976157
GGGCCCG12250.067.437258
AACGGGC12100.065.557845
ACCTTCA74900.063.1060374
CGGGGCA13950.062.9202894
GCGTAGT19950.062.5838133
ACGGGCC13050.062.2241336
GCAGCCA2200.061.876022
GGCCCGA13550.060.620859
GCTGCCA2250.060.5009962
TGTGGCA68500.058.7237138
GCCTTCA56250.058.1612244
TGTGTGG64000.056.692136
Produced by FastQC (version 0.11.5)