FastQCFastQC Report
Tue 7 Mar 2017
1HL_19A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_19A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3617984
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA182530.5045074826201553No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG108990.3012451133006669No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA92360.2552802886911606No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT76300.21089092710194407No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA71700.19817666413118468No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG64320.17777856397374892No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG63010.17415776299729352No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61510.17001180768074156No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA58420.16147113972864446No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG57370.15856897100705808No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT55990.15475469211583026No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC54280.150028303054961No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC51500.14234446586828467No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45860.1267556738780492No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45780.12653455626116644No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA45650.12617524013373194No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA45310.12523549026198016No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG43800.12106189524331783No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41280.1140966903115105No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA40630.11230010967433798No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA39920.11033769082450337No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA36500.10088491270276487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAT258.3598206E-786.03
GCAGCTA258.3598206E-786.03
GCAGCAC357.2759576E-1086.03
AGCAGCA1050.086.02
CAGCAGC3900.083.794871
AGCTGCT1900.083.736842
AGCTGCA1600.083.31252
CAGCTGC6000.083.133341
GCTGCCC1100.082.090913
AGCAGCT950.081.473692
AGCTGCC1750.081.085722
CAGCCCT211200.080.584281
GCTGCCA750.080.266673
AGCAGCG1500.080.266672
CTGCTGC2100.079.857151
TGCTGCC700.079.857142
GCTGCAC650.079.384613
CAGCGTA82100.079.03411
AGCCCTT178950.077.8301242
GCTGCAA1000.077.43
Produced by FastQC (version 0.11.5)