##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_19A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3617984 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.862828580778688 12.0 12.0 12.0 2.0 32.0 2 14.486079264032124 12.0 12.0 12.0 12.0 22.0 3 13.729814172754772 12.0 12.0 12.0 12.0 22.0 4 13.742322519944809 12.0 12.0 12.0 12.0 22.0 5 22.444649561744882 22.0 22.0 22.0 22.0 27.0 6 26.811423986396843 27.0 22.0 32.0 22.0 32.0 7 34.954517488192316 37.0 37.0 37.0 27.0 41.0 8 37.59893686649803 41.0 37.0 41.0 27.0 41.0 9 38.54326525490439 41.0 41.0 41.0 32.0 41.0 10-11 38.8953151810511 41.0 41.0 41.0 37.0 41.0 12-13 39.078116984486385 41.0 41.0 41.0 37.0 41.0 14-15 39.03254616383046 41.0 41.0 41.0 37.0 41.0 16-17 38.94214333175603 41.0 41.0 41.0 37.0 41.0 18-19 38.86863982814739 41.0 41.0 41.0 37.0 41.0 20-21 38.87714594647185 41.0 41.0 41.0 37.0 41.0 22-23 38.866978958447575 41.0 41.0 41.0 37.0 41.0 24-25 38.87985173510994 41.0 41.0 41.0 37.0 41.0 26-27 38.84193559175497 41.0 41.0 41.0 37.0 41.0 28-29 38.79172171574004 41.0 41.0 41.0 34.5 41.0 30-31 38.67374081809096 41.0 41.0 41.0 32.0 41.0 32-33 38.72280322411597 41.0 41.0 41.0 32.0 41.0 34-35 38.69221091082768 41.0 41.0 41.0 32.0 41.0 36-37 38.625011885071906 41.0 41.0 41.0 32.0 41.0 38-39 38.518387726424436 41.0 41.0 41.0 32.0 41.0 40-41 38.57237317799084 41.0 41.0 41.0 32.0 41.0 42-43 38.312461028020024 41.0 41.0 41.0 32.0 41.0 44-45 38.425943978746176 41.0 41.0 41.0 32.0 41.0 46-47 38.434998330561996 41.0 41.0 41.0 32.0 41.0 48-49 38.38243549446321 41.0 41.0 41.0 32.0 41.0 50-51 38.269495249288 41.0 41.0 41.0 32.0 41.0 52-53 38.233628037050465 41.0 41.0 41.0 32.0 41.0 54-55 38.10528708805788 41.0 37.0 41.0 32.0 41.0 56-57 37.89948905246679 41.0 37.0 41.0 29.5 41.0 58-59 37.88912706634413 41.0 37.0 41.0 29.5 41.0 60-61 37.88841188905202 41.0 37.0 41.0 29.5 41.0 62-63 37.87812342453698 41.0 37.0 41.0 29.5 41.0 64-65 37.853714389007806 41.0 37.0 41.0 29.5 41.0 66-67 37.73585869920929 41.0 37.0 41.0 27.0 41.0 68-69 37.76129841923016 41.0 37.0 41.0 27.0 41.0 70-71 37.73047075387841 41.0 37.0 41.0 27.0 41.0 72-73 37.59568685212538 41.0 37.0 41.0 27.0 41.0 74-75 37.510566796315295 41.0 37.0 41.0 27.0 41.0 76-77 36.37717220418885 39.0 34.5 41.0 24.5 41.0 78-79 36.95247961295573 41.0 37.0 41.0 27.0 41.0 80-81 37.63201467999858 41.0 37.0 41.0 27.0 41.0 82-83 37.81523757429552 41.0 37.0 41.0 27.0 41.0 84-85 37.74519096822982 41.0 37.0 41.0 27.0 41.0 86-87 37.60877618585378 41.0 37.0 41.0 27.0 41.0 88-89 37.52560763121119 41.0 37.0 41.0 27.0 41.0 90-91 37.36551046107445 41.0 37.0 41.0 27.0 41.0 92-93 37.25111802595036 41.0 37.0 41.0 27.0 41.0 94-95 37.09486291260548 41.0 37.0 41.0 27.0 41.0 96-97 37.010279619810376 41.0 37.0 41.0 27.0 41.0 98-99 36.88658697772019 41.0 37.0 41.0 27.0 41.0 100 36.75611141453362 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3506759921260283 1101 2 -0.4550134569214279 1101 3 -0.02182047045370794 1101 4 0.06621549300152196 1101 5 -1.052718027936148 1101 6 -0.8998861695061642 1101 7 -0.22923652499346048 1101 8 -0.17678654845773423 1101 9 -0.07812691466602217 1101 10-11 -0.018667434434583186 1101 12-13 -0.08390401397488745 1101 14-15 0.02611213613643315 1101 16-17 -0.12868040399747116 1101 18-19 0.01065467976981438 1101 20-21 0.05552427586118114 1101 22-23 0.12771668641634193 1101 24-25 0.07274728181946699 1101 26-27 0.07055632772105014 1101 28-29 0.05244941314423812 1101 30-31 -0.044945678056087957 1101 32-33 -0.08497022624167272 1101 34-35 0.05243536018165429 1101 36-37 0.03691758106013765 1101 38-39 0.037007765993834596 1101 40-41 0.019335950777772837 1101 42-43 0.19306707841238335 1101 44-45 0.03816977050492909 1101 46-47 0.02392879756041566 1101 48-49 0.15609737124056977 1101 50-51 0.023840423698182178 1101 52-53 0.08646304216181022 1101 54-55 -0.009821807596317456 1101 56-57 -6.803054591131286E-4 1101 58-59 -0.0669309835252534 1101 60-61 -0.08088978916055822 1101 62-63 -0.09166647547469609 1101 64-65 -0.06083678857326191 1101 66-67 -0.09621571123796002 1101 68-69 -0.021054910870198285 1101 70-71 0.03511062792836839 1101 72-73 -0.02330812764178347 1101 74-75 -0.003429155251083671 1101 76-77 -0.5223419831270775 1101 78-79 -0.15450669568580366 1101 80-81 -0.11509551333640644 1101 82-83 -0.21493640104464617 1101 84-85 -0.17183732679878005 1101 86-87 -0.21159467396869758 1101 88-89 -0.4281155464592814 1101 90-91 -0.3825763923330001 1101 92-93 -0.21175074168097296 1101 94-95 -0.05431717700123784 1101 96-97 -0.18057700077273608 1101 98-99 -0.4884656240695051 1101 100 -0.4727926200338999 1102 1 1.6500830115446092 1102 2 0.7199785870367972 1102 3 -0.15743521701363328 1102 4 0.050240637013176936 1102 5 -0.7565999923923492 1102 6 -0.11210723360120767 1102 7 0.01982524600683888 1102 8 -0.15708884884867302 1102 9 -0.20340026674349332 1102 10-11 -0.1904358946412401 1102 12-13 -0.17949140342970082 1102 14-15 -0.06750565853637625 1102 16-17 -0.1417071450567775 1102 18-19 -0.01749437995742653 1102 20-21 0.03860447568191461 1102 22-23 -0.10153789816148873 1102 24-25 -0.1886337994431031 1102 26-27 -0.14926341318935954 1102 28-29 -0.3263654723909397 1102 30-31 -0.32439179156617826 1102 32-33 -0.4353389778292396 1102 34-35 -0.15866645671551538 1102 36-37 -0.33996376735025535 1102 38-39 -0.11438154867711603 1102 40-41 -0.2955457888655033 1102 42-43 -0.1301657314280007 1102 44-45 -0.09645244159585076 1102 46-47 -0.466877083779238 1102 48-49 -0.32745041026462474 1102 50-51 -0.2574717830596356 1102 52-53 -0.30651403567893 1102 54-55 -0.19228946519163514 1102 56-57 -0.1798103670344915 1102 58-59 -0.2234038439339443 1102 60-61 -0.06896200374303874 1102 62-63 -0.4744020108068199 1102 64-65 -0.21864407426377142 1102 66-67 -0.40470561071447264 1102 68-69 -0.6131579919751076 1102 70-71 -0.3687219944705973 1102 72-73 -0.25417479606024074 1102 74-75 -0.3493815007505958 1102 76-77 -0.2477239461234575 1102 78-79 -0.3224425334935006 1102 80-81 -0.24457856039821735 1102 82-83 -0.3665922796106287 1102 84-85 -0.3835529997631326 1102 86-87 -0.10474167534385259 1102 88-89 -0.3024997196294592 1102 90-91 -0.4379348229636193 1102 92-93 -0.26990999098099167 1102 94-95 -0.29724272304846977 1102 96-97 -0.24379585469397114 1102 98-99 -0.4003011485989276 1102 100 -0.4698163304982117 1103 1 1.538281285106235 1103 2 0.7257923487715114 1103 3 0.359320949801452 1103 4 1.013260734818699 1103 5 2.929734219451653 1103 6 -0.23424300397710596 1103 7 -0.4708868273682896 1103 8 -0.14464338097427998 1103 9 -0.13987764884166864 1103 10-11 -0.12100416811291836 1103 12-13 -0.18465317832109207 1103 14-15 -0.17944156023912683 1103 16-17 -0.3706498739567792 1103 18-19 -0.19953273459389465 1103 20-21 -0.13196886496734095 1103 22-23 -0.07002664992675278 1103 24-25 -0.2491507415063765 1103 26-27 -0.046607792370153334 1103 28-29 -0.23068801473485934 1103 30-31 -0.13444234340880712 1103 32-33 -0.2974990046942736 1103 34-35 -0.46458620941326245 1103 36-37 -0.11482067205433566 1103 38-39 -0.17656601193107946 1103 40-41 -0.3838753892780673 1103 42-43 -0.10281946329934044 1103 44-45 -0.38271094560911223 1103 46-47 -0.483222029644395 1103 48-49 -0.40894651693320583 1103 50-51 -0.2094136514389504 1103 52-53 -0.19502773354922454 1103 54-55 -0.30153466561500153 1103 56-57 -0.23239777584778665 1103 58-59 -0.5400054468411213 1103 60-61 -0.2827472753467575 1103 62-63 -0.17521155988798398 1103 64-65 -0.3608347740439939 1103 66-67 -0.5021538820273506 1103 68-69 -0.3636680078962371 1103 70-71 -0.5171858001308394 1103 72-73 -0.22175790227007752 1103 74-75 -0.4434145243564487 1103 76-77 -0.5140721901515377 1103 78-79 -0.45398556402548707 1103 80-81 -0.21060013638039976 1103 82-83 -0.31515772865551384 1103 84-85 -0.47511989700137036 1103 86-87 -0.31435881859506054 1103 88-89 -0.11364121381720338 1103 90-91 -0.6840825686692611 1103 92-93 -0.32218242523511265 1103 94-95 -0.3530929144918389 1103 96-97 -0.29338733224097524 1103 98-99 -0.2308165015473307 1103 100 -0.2745794774253767 1104 1 1.6952207961176775 1104 2 -0.5062345717716799 1104 3 0.04580201288084851 1104 4 0.0166939133905597 1104 5 -1.0632609984779613 1104 6 -0.48053830921859486 1104 7 -0.2980617038250415 1104 8 -0.17330164779203727 1104 9 -0.02627465533752371 1104 10-11 -0.08836783733904241 1104 12-13 0.009613485581084547 1104 14-15 -0.046979973406678255 1104 16-17 0.009900382154377496 1104 18-19 0.13514848892620535 1104 20-21 0.099437096579571 1104 22-23 0.09197538718334641 1104 24-25 0.05935474906525684 1104 26-27 0.041425903582407386 1104 28-29 0.015869416435045025 1104 30-31 -0.10355545530432408 1104 32-33 -0.009654285810562158 1104 34-35 -0.1459645310480866 1104 36-37 -0.20310317228896935 1104 38-39 -0.046539581985058476 1104 40-41 -0.012094332238490324 1104 42-43 -0.04327921635207588 1104 44-45 -0.06498958016239698 1104 46-47 -0.11168967672413288 1104 48-49 -0.32861754037920576 1104 50-51 -0.2100210383819885 1104 52-53 -0.07437273123595389 1104 54-55 -0.17534171468903992 1104 56-57 -0.13361175844811157 1104 58-59 -0.21776373628564016 1104 60-61 -0.1695887039841466 1104 62-63 -0.1494477963486105 1104 64-65 -0.23147968495973714 1104 66-67 -0.4870300573245885 1104 68-69 -0.3380945535903379 1104 70-71 -0.24447467181844473 1104 72-73 -0.17554956834829483 1104 74-75 -0.2201015130443693 1104 76-77 -0.2752802771721292 1104 78-79 -0.22383919919728612 1104 80-81 -0.2493544081966732 1104 82-83 -0.1778403015682386 1104 84-85 -0.08555414160542796 1104 86-87 -0.20949607712645246 1104 88-89 -0.31358910915233196 1104 90-91 -0.31074793722662974 1104 92-93 -0.3805120550506018 1104 94-95 -0.2469692741887073 1104 96-97 -0.4300779908922152 1104 98-99 -0.4371936938470995 1104 100 -0.2656566787698367 1105 1 1.5963487588399143 1105 2 0.3022126824000555 1105 3 -0.13092246757484816 1105 4 -0.24888838966026228 1105 5 -1.128105123444854 1105 6 0.3327799348937788 1105 7 -0.05899315328076682 1105 8 -0.20640281149036355 1105 9 -0.12316282942899193 1105 10-11 -0.10740275346531547 1105 12-13 -0.2051740691857944 1105 14-15 -0.11241803035570541 1105 16-17 -0.018769680865098337 1105 18-19 -0.06607151599894223 1105 20-21 -0.16744857851145412 1105 22-23 -0.1000765835905284 1105 24-25 -0.09553515831835568 1105 26-27 -0.1466782312136985 1105 28-29 -0.21317552540879348 1105 30-31 -0.1560476930178325 1105 32-33 -0.17934087357834017 1105 34-35 -0.0812139628298354 1105 36-37 -0.23220162169130276 1105 38-39 -0.2047362794893388 1105 40-41 -0.042006849968494464 1105 42-43 0.003020686132259698 1105 44-45 -0.11419228207362409 1105 46-47 -0.27291100831465087 1105 48-49 -0.20809425716232255 1105 50-51 -0.25114617906459813 1105 52-53 -0.18937223613288268 1105 54-55 -0.2870669250982658 1105 56-57 0.06108683305312468 1105 58-59 -0.11629684123570883 1105 60-61 -0.3283179368542761 1105 62-63 -0.409615502571981 1105 64-65 -0.3145787350040763 1105 66-67 -0.2536593140727774 1105 68-69 -0.36581491016808343 1105 70-71 -0.46882797277564947 1105 72-73 -0.3514080258064709 1105 74-75 -0.3574493383436277 1105 76-77 -0.44129131169218283 1105 78-79 -0.18951321853611347 1105 80-81 -0.4834942626910035 1105 82-83 -0.29693622129578046 1105 84-85 -0.268023639085861 1105 86-87 -0.288748324812957 1105 88-89 -0.16569951838545904 1105 90-91 -0.2991059693179352 1105 92-93 -0.18832109115631113 1105 94-95 -0.170623804008045 1105 96-97 -0.3192772764484033 1105 98-99 -0.46842516043253823 1105 100 -0.49435241238079897 1106 1 1.308629182774359 1106 2 -0.6550784876197433 1106 3 -0.14278075709882643 1106 4 -0.28619351183752784 1106 5 -1.13147211626314 1106 6 -0.520843268371955 1106 7 -0.16364477751658768 1106 8 -0.02181584414977067 1106 9 0.018925027010368467 1106 10-11 -0.1959653451640193 1106 12-13 -0.12994511082600013 1106 14-15 -0.1341789718719042 1106 16-17 -0.1941797058738004 1106 18-19 -0.05298614675760405 1106 20-21 -0.1210308822845505 1106 22-23 -0.16840569880765344 1106 24-25 -0.2142931200957463 1106 26-27 -0.17813827664365078 1106 28-29 -0.12317969510636573 1106 30-31 -0.16747116435183074 1106 32-33 -0.20273157632998107 1106 34-35 -0.3259922995420297 1106 36-37 -0.24453783147038166 1106 38-39 -0.1380650747638441 1106 40-41 -0.1819085582723332 1106 42-43 -0.046971111183978564 1106 44-45 -0.11386180126154244 1106 46-47 -0.24976277841393824 1106 48-49 -0.1987853503579018 1106 50-51 -0.20968194494937364 1106 52-53 -0.20584994676384127 1106 54-55 -0.19941498319833073 1106 56-57 -0.264088105317569 1106 58-59 -0.39800258742454275 1106 60-61 -0.21043317164944852 1106 62-63 -0.328668305260031 1106 64-65 -0.4106796310523322 1106 66-67 -0.4391633870280529 1106 68-69 -0.17284421140765716 1106 70-71 -0.48079639092416215 1106 72-73 -0.5383464766271118 1106 74-75 -0.49215079198450695 1106 76-77 -0.4508863155742091 1106 78-79 -0.4143824360475534 1106 80-81 -0.41256473532698834 1106 82-83 -0.3746425515460814 1106 84-85 -0.7535401110052433 1106 86-87 -0.4722131669141376 1106 88-89 -0.5914844554099261 1106 90-91 -0.5762690521523197 1106 92-93 -0.5097411655918691 1106 94-95 -0.448431898803733 1106 96-97 -0.37205893562727965 1106 98-99 -0.6770259591688657 1106 100 -0.8141559694973992 1107 1 1.3618541854423398 1107 2 -0.43426653444682195 1107 3 -0.1631857245978825 1107 4 -0.16727323923285553 1107 5 -1.1638747649916787 1107 6 -0.3194554729838899 1107 7 -0.4685762709407655 1107 8 -0.1773028489052635 1107 9 -0.08136247574234545 1107 10-11 -0.1530233498708995 1107 12-13 -0.31758588190822934 1107 14-15 -0.2902185536642463 1107 16-17 -0.24722003198915132 1107 18-19 -0.07297033836722733 1107 20-21 -0.3472665349888189 1107 22-23 -0.12833795631049583 1107 24-25 -0.21598271202739028 1107 26-27 -0.31741141607338363 1107 28-29 -0.3366320407116419 1107 30-31 -0.3177585605790014 1107 32-33 -0.24812332013566873 1107 34-35 -0.26469605026760945 1107 36-37 -0.24349397800381922 1107 38-39 -0.2722911308725884 1107 40-41 -0.29285171411280686 1107 42-43 -0.3485650141268124 1107 44-45 -0.21200949640714128 1107 46-47 -0.28423158686376837 1107 48-49 -0.27588902159606477 1107 50-51 -0.3843233557658934 1107 52-53 -0.17641577751490445 1107 54-55 -0.3353335364807606 1107 56-57 -0.26966941422982416 1107 58-59 -0.35679342374882594 1107 60-61 -0.1886534920356695 1107 62-63 -0.32098239059008904 1107 64-65 -0.4018086681524693 1107 66-67 -0.38869507169463446 1107 68-69 -0.4185143839267411 1107 70-71 -0.2757109003033449 1107 72-73 -0.3095538719788422 1107 74-75 -0.28372785614445206 1107 76-77 -0.4646703817161182 1107 78-79 -0.9163958172050428 1107 80-81 -0.493383969036465 1107 82-83 -0.31727753054408936 1107 84-85 -0.34470302322761626 1107 86-87 -0.3098994728233464 1107 88-89 -0.22292065600410638 1107 90-91 -0.11692220092143657 1107 92-93 -0.21576892304909734 1107 94-95 -0.21129848950800323 1107 96-97 -0.4273334412955023 1107 98-99 -0.4718342335306218 1107 100 -0.8078460743010965 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9 0.14929513731235033 2223 10-11 0.1408361829289504 2223 12-13 0.17542930541446822 2223 14-15 0.08114394893645027 2223 16-17 0.2131413726924265 2223 18-19 0.09285537542142919 2223 20-21 0.08543768663324869 2223 22-23 0.25756127506952 2223 24-25 0.21097110193224466 2223 26-27 0.1454061601688963 2223 28-29 0.063669061238393 2223 30-31 0.05656790237404863 2223 32-33 0.06629651237972922 2223 34-35 0.13692945791613198 2223 36-37 0.11379726488716102 2223 38-39 0.14490276899799426 2223 40-41 0.12802079408616152 2223 42-43 0.2925469394425164 2223 44-45 0.1559689124919501 2223 46-47 0.16501438005235514 2223 48-49 0.2372758588176822 2223 50-51 0.2887321367834872 2223 52-53 0.3081095182372451 2223 54-55 0.23502405463853648 2223 56-57 0.2795763324705547 2223 58-59 0.45596545542196054 2223 60-61 0.2553707286327622 2223 62-63 0.22977192973171867 2223 64-65 0.31674746718753966 2223 66-67 0.23327304815022387 2223 68-69 0.4354632343020697 2223 70-71 0.37509533348745805 2223 72-73 0.4175274851974109 2223 74-75 0.33941564132014435 2223 76-77 0.4617156585969582 2223 78-79 0.45630947485705065 2223 80-81 0.38044303502498167 2223 82-83 0.4150416582040748 2223 84-85 0.29874551517652037 2223 86-87 0.4171926079023862 2223 88-89 0.3574198415858234 2223 90-91 0.4507556039067069 2223 92-93 0.47046049790891686 2223 94-95 0.5364002319863843 2223 96-97 0.6093011718519747 2223 98-99 0.5321212721646802 2223 100 0.6430663513533474 2224 1 1.4581451019947114 2224 2 -0.8338431753130031 2224 3 -0.04183951853443446 2224 4 -0.32479868745009277 2224 5 -1.03765480877588 2224 6 0.5727194702031007 2224 7 0.45302775356481106 2224 8 0.37349369032232715 2224 9 0.24005211420888628 2224 10-11 0.31488115644603454 2224 12-13 0.27442694363842435 2224 14-15 0.22771939248951867 2224 16-17 0.34416290389738435 2224 18-19 0.17181886145257863 2224 20-21 0.24397562188450905 2224 22-23 0.2962771962939499 2224 24-25 0.29055435180836753 2224 26-27 0.21051581351905213 2224 28-29 0.19233714642648891 2224 30-31 0.17431625594544187 2224 32-33 0.2160822658212993 2224 34-35 0.17476940796229457 2224 36-37 0.27837936832489163 2224 38-39 0.2479373669652034 2224 40-41 0.31843597609314145 2224 42-43 0.42216074218433164 2224 44-45 0.42345120387781066 2224 46-47 0.3543412456408319 2224 48-49 0.22583759443129026 2224 50-51 0.2637975722493593 2224 52-53 0.280909137747706 2224 54-55 0.34877851050008957 2224 56-57 0.30248566368846497 2224 58-59 0.32053616036297683 2224 60-61 0.29452225879858673 2224 62-63 0.38063065727169487 2224 64-65 0.3897863233545067 2224 66-67 0.36236149408426854 2224 68-69 0.510277469866736 2224 70-71 0.39501663020905653 2224 72-73 0.5511871424631565 2224 74-75 0.33445978955218436 2224 76-77 0.6111118771197965 2224 78-79 0.33473523609967515 2224 80-81 0.3431144172117726 2224 82-83 0.26050755081461574 2224 84-85 0.3304189820363561 2224 86-87 0.34419733145448816 2224 88-89 0.3200255247576962 2224 90-91 0.3557437216092225 2224 92-93 0.3786130397266163 2224 94-95 0.570522822583257 2224 96-97 0.415231411546074 2224 98-99 0.359214124638946 2224 100 0.7243212552482987 2225 1 1.4397629436725836 2225 2 1.5840671497012533 2225 3 0.7640729269599991 2225 4 1.1465675513897846 2225 5 3.171864173454132 2225 6 0.1510175768956188 2225 7 0.5166899907167775 2225 8 0.5215677135326615 2225 9 0.38988598850161793 2225 10-11 0.3026371605659932 2225 12-13 0.3437973769302616 2225 14-15 0.2683004222080001 2225 16-17 0.2727562632552534 2225 18-19 0.29069851777446587 2225 20-21 0.30200183598041264 2225 22-23 0.33928479190840477 2225 24-25 0.252791522676425 2225 26-27 0.2635151983226791 2225 28-29 0.20776398948220987 2225 30-31 0.2661199459136938 2225 32-33 0.33892747152451363 2225 34-35 0.29489902458188766 2225 36-37 0.3004971808877599 2225 38-39 0.3767804537007038 2225 40-41 0.270397202840833 2225 42-43 0.6141659118000575 2225 44-45 0.3801276442389323 2225 46-47 0.47102512923524387 2225 48-49 0.4030509685054753 2225 50-51 0.44259038832870345 2225 52-53 0.4537042145886403 2225 54-55 0.38118606845408465 2225 56-57 0.5019833636027045 2225 58-59 0.6119807147974541 2225 60-61 0.5289126434343316 2225 62-63 0.6015911292978373 2225 64-65 0.5698240896812194 2225 66-67 0.45128388519034957 2225 68-69 0.5999511322022002 2225 70-71 0.5677087174300652 2225 72-73 0.6200380902512492 2225 74-75 0.48135938133705736 2225 76-77 0.7384753447401238 2225 78-79 0.6776958695817825 2225 80-81 0.5000795782669272 2225 82-83 0.5375740642430529 2225 84-85 0.4828206961920003 2225 86-87 0.5202783868849252 2225 88-89 0.5843772393670719 2225 90-91 0.6255179176946513 2225 92-93 0.6291489733201985 2225 94-95 0.8387406418009746 2225 96-97 0.8703143723081155 2225 98-99 0.9210436759199396 2225 100 1.0576893438365786 2226 1 1.5781285801165463 2226 2 1.3088069682065377 2226 3 0.9000069916946636 2226 4 0.9096468588758828 2226 5 3.171679100665955 2226 6 -0.286312648480326 2226 7 0.10288099817235974 2226 8 0.23241169764677494 2226 9 0.40453378786752836 2226 10-11 0.2809441146997429 2226 12-13 0.19171310142616704 2226 14-15 0.2647710366828733 2226 16-17 0.2782322348755244 2226 18-19 0.21925202208025496 2226 20-21 0.20192211231780988 2226 22-23 0.27790231716253544 2226 24-25 0.2668008209231232 2226 26-27 0.24221607276787438 2226 28-29 0.17198035570614678 2226 30-31 0.3044017776028056 2226 32-33 0.1884542783687877 2226 34-35 0.25241295605466263 2226 36-37 0.29213549740872935 2226 38-39 0.22858840129568847 2226 40-41 0.3012802040950575 2226 42-43 0.39374958344485833 2226 44-45 0.2988715314982855 2226 46-47 0.2113904534281943 2226 48-49 0.2449036610060844 2226 50-51 0.20405975237590468 2226 52-53 0.3046489162631758 2226 54-55 0.30911962637403434 2226 56-57 0.4796131886045174 2226 58-59 0.43755302866762236 2226 60-61 0.2837950296774423 2226 62-63 0.2784260429471459 2226 64-65 0.45997423116699565 2226 66-67 0.48176513613054084 2226 68-69 0.4946713377373939 2226 70-71 0.6115560200416326 2226 72-73 0.3041420461109112 2226 74-75 0.4605502715798764 2226 76-77 0.6717096245863132 2226 78-79 0.42455848245839434 2226 80-81 0.37345747459675493 2226 82-83 0.33851636946225483 2226 84-85 0.36315671244803127 2226 86-87 0.5503128056780682 2226 88-89 0.4742272782086303 2226 90-91 0.40401748989178543 2226 92-93 0.3585051240719537 2226 94-95 0.6651266115752676 2226 96-97 0.5628299812996147 2226 98-99 0.41719368738021245 2226 100 0.6940571907139983 2227 1 1.4245879665214574 2227 2 1.4421777791073094 2227 3 0.73199527751202 2227 4 -0.10600814199171182 2227 5 3.250386720321913 2227 6 0.10770657476950873 2227 7 0.31368191333105955 2227 8 0.37460918488461914 2227 9 0.2745236651418921 2227 10-11 0.2151920708878592 2227 12-13 0.2526645808996264 2227 14-15 0.1716071151928631 2227 16-17 0.2736520443072976 2227 18-19 0.26199294980607135 2227 20-21 0.20578245936513184 2227 22-23 0.2561048879849679 2227 24-25 0.2637923714495116 2227 26-27 0.3683962024390013 2227 28-29 0.24145681552280251 2227 30-31 0.2783268940188819 2227 32-33 0.26974269661844374 2227 34-35 0.2712652914445286 2227 36-37 0.24944738184795767 2227 38-39 0.15280058043341427 2227 40-41 0.2520307562114894 2227 42-43 0.46101318044974704 2227 44-45 0.2688948832693754 2227 46-47 0.33377184236449153 2227 48-49 0.23718537820994356 2227 50-51 0.31904356121571453 2227 52-53 0.3269532854887629 2227 54-55 0.3049689779002236 2227 56-57 0.33183694953987697 2227 58-59 0.4375081596034889 2227 60-61 0.34630836864975123 2227 62-63 0.42561666744036586 2227 64-65 0.4338549513225729 2227 66-67 0.470941242715611 2227 68-69 0.30786794887011837 2227 70-71 0.5499780757634269 2227 72-73 0.45362751756468356 2227 74-75 0.3223423013400293 2227 76-77 0.5212535818486472 2227 78-79 0.3478033999376038 2227 80-81 0.3354819818999104 2227 82-83 0.3125458336386302 2227 84-85 0.3038162167701657 2227 86-87 0.34409250661087043 2227 88-89 0.39496737663689174 2227 90-91 0.3533558248570827 2227 92-93 0.45008940864677527 2227 94-95 0.6063745672905867 2227 96-97 0.4602437927445848 2227 98-99 0.7122427714886683 2227 100 0.5001404712903224 2228 1 1.3329780343657358 2228 2 1.6926656840446928 2228 3 -0.2468940303150884 2228 4 -0.06352448651069409 2228 5 3.170466587336694 2228 6 0.2604407632110828 2228 7 0.22863492506211713 2228 8 0.5041268289025354 2228 9 0.3389253146518527 2228 10-11 0.30691250787995017 2228 12-13 0.32243354042308425 2228 14-15 0.34475761756302603 2228 16-17 0.22976644764046483 2228 18-19 0.2432264778271147 2228 20-21 0.2663252610184301 2228 22-23 0.35368487949553895 2228 24-25 0.36770685114009893 2228 26-27 0.31748439525808436 2228 28-29 0.21524359810097593 2228 30-31 0.22847280308715057 2228 32-33 0.30133783156002636 2228 34-35 0.35593978830367945 2228 36-37 0.34670434827678065 2228 38-39 0.3088757142367484 2228 40-41 0.2690865374403728 2228 42-43 0.4762589588567252 2228 44-45 0.41691928918731236 2228 46-47 0.2776864169090203 2228 48-49 0.2988873423978404 2228 50-51 0.22687944493427636 2228 52-53 0.38339830221862314 2228 54-55 0.3111322547243205 2228 56-57 0.5350240892431231 2228 58-59 0.5117022924132328 2228 60-61 0.3847748650536005 2228 62-63 0.5294551217006074 2228 64-65 0.39037860196603447 2228 66-67 0.40073306203782977 2228 68-69 0.549724714682867 2228 70-71 0.6135166493025039 2228 72-73 0.674195123522459 2228 74-75 0.45578983881325996 2228 76-77 0.5548870804649653 2228 78-79 0.7162331167274658 2228 80-81 0.5080440578731711 2228 82-83 0.40035838328874007 2228 84-85 0.3920015898188396 2228 86-87 0.4455097192805013 2228 88-89 0.4661288393794507 2228 90-91 0.5866634527735428 2228 92-93 0.4995640364043652 2228 94-95 0.7986204950925213 2228 96-97 0.6369981755892979 2228 98-99 0.729422099970229 2228 100 0.6808436709507504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 22.0 13 42.0 14 142.0 15 641.0 16 2325.0 17 5917.0 18 9732.0 19 12372.0 20 14750.0 21 16973.0 22 19425.0 23 22103.0 24 25248.0 25 28517.0 26 32405.0 27 36431.0 28 41732.0 29 47070.0 30 53995.0 31 61920.0 32 72868.0 33 86910.0 34 107162.0 35 137360.0 36 194199.0 37 323468.0 38 799278.0 39 1463492.0 40 1485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.7584858492551 17.820260057634734 22.555160552782226 33.866093540327945 2 21.306649035120646 49.44929981496828 12.76016803777367 16.483883112137406 3 38.53939099785958 15.798273292529764 25.191239098901484 20.471096610709168 4 18.492038660204134 15.805929490014329 22.85991314500009 42.84211870478145 5 17.785540234561566 35.29697754329483 26.179994162494914 20.737488059648687 6 17.6531184217509 27.912616528984096 30.709533264934286 23.72473178433072 7 20.417309750402435 26.58873007730272 35.853281827669775 17.140678344625073 8 21.250259813199836 27.820686879765084 33.63397958642161 17.29507372061347 9 20.93934080416055 28.202197687994197 27.57859072898056 23.279870778864694 10-11 19.710880424015144 30.048820005837506 28.7431619376979 21.497137632449455 12-13 24.271223974456493 26.6897946480692 27.199249637367107 21.839731740107197 14-15 21.7287445162831 27.303865909854768 28.61923933328616 22.34815024057597 16-17 20.91834845040774 29.469947904689462 28.32059235198387 21.29111129291893 18-19 21.458870464877677 29.475296187047817 29.679760883409102 19.386072464665403 20-21 22.89552690116927 28.912814429251206 29.745985609665492 18.445673059914032 22-23 21.92218097150236 30.295960402257165 28.620137623604748 19.161721002635723 24-25 22.23569811254002 28.354865582600695 26.605189519909427 22.80424678494985 26-27 20.328835063947214 26.478599684244042 29.949151240027593 23.243414011781148 28-29 19.877179931152103 27.931325129205547 30.561553001118995 21.629941938523352 30-31 20.212875057185467 29.93159858083295 28.226428185420506 21.629098176561076 32-33 20.85968318267853 29.891135505297978 28.645206833418836 20.60397447860466 34-35 21.449279488245388 29.16010684403248 29.725546049954893 19.665067617767242 36-37 21.88287731510145 28.07187649254391 28.79306541985813 21.252180772496505 38-39 21.53663857944079 29.294583639848526 28.866372181747398 20.302405598963283 40-41 20.98610630673037 29.59033415871174 29.817853353700375 19.605706180857513 42-43 20.59533504819472 29.825238092145078 29.238805803073237 20.340621056586972 44-45 20.558410429675753 30.19829551485026 28.63716368010472 20.606130375369265 46-47 20.120627771889627 27.877945436124456 28.90052575985587 23.100901032130043 48-49 21.0234098635331 28.17514231339087 30.05923050782294 20.742217315253093 50-51 21.862188998071854 30.681672445207052 28.117564920132317 19.338573636588773 52-53 20.347228940305644 30.268205852517877 28.26062140505759 21.12394380211889 54-55 22.42714000638316 28.287910577175467 28.105358881263715 21.17959053517766 56-57 21.474409298633002 28.692443312682236 29.209061677968933 20.624085710715832 58-59 21.182184509054782 27.857768983444558 29.04428523605238 21.915761271448282 60-61 19.67425170554543 30.280882067398153 29.50000380046587 20.544862426590544 62-63 20.385189771856364 31.02067895070375 27.374928042116697 21.219203235323185 64-65 19.90208624702182 30.25970292815328 28.76279028629235 21.07542053853255 66-67 20.30715171764165 30.149483800923388 29.40491168562382 20.138452795811148 68-69 20.86615087297235 29.943830597371353 29.227672095841218 19.962346433815075 70-71 19.71806674656383 30.77521901699952 29.195499482584776 20.31121475385187 72-73 20.123859033096885 30.726794258902196 28.42225117634572 20.7270955316552 74-75 20.112982810316463 29.172406511471582 30.345200531566753 20.369410146645205 76-77 19.57196328120854 30.427456285047143 28.65823895296386 21.34234148078046 78-79 19.673400987953514 29.377949156215173 28.543078134121103 22.405571721710213 80-81 21.146181968542088 29.238591068986896 28.12305053348874 21.492176428982273 82-83 20.465002056393836 27.892715943464648 29.819078801896303 21.82320319824521 84-85 20.744563823388937 29.156444583502854 28.16155350604093 21.937438087067274 86-87 21.860309498328352 28.533722094956747 28.661666276025542 20.94430213068936 88-89 21.286025587730624 28.489498571580196 28.861169645858027 21.363306194831154 90-91 20.76024935433656 29.142306875873413 27.80841485202809 22.289028917761936 92-93 20.812502211176167 29.08704129150378 29.050667443526564 21.049789053793493 94-95 20.654831530487698 29.60346148573349 27.940684646477155 21.801022337301657 96-97 20.100807521536858 29.759985118784382 28.354423347366932 21.784784012311828 98-99 21.976175129574923 30.03292717712406 27.352373587058427 20.638524106242592 100 21.060983133148184 31.568934522651286 26.523970255258178 20.846112088942352 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 9.5 2 13.0 3 20.0 4 34.0 5 34.0 6 40.0 7 48.5 8 59.0 9 86.0 10 109.0 11 120.0 12 135.0 13 139.0 14 166.5 15 226.5 16 296.5 17 408.0 18 586.0 19 926.0 20 1532.5 21 2359.0 22 3568.5 23 5495.5 24 8520.0 25 12991.5 26 18748.0 27 25248.0 28 32863.0 29 41613.0 30 51326.0 31 62484.5 32 75012.5 33 89305.5 34 104807.0 35 121560.0 36 138708.0 37 152439.5 38 162223.0 39 169374.0 40 173819.0 41 176068.0 42 175385.5 43 173229.5 44 171074.5 45 165379.5 46 154179.5 47 141482.0 48 131981.0 49 127346.0 50 123972.0 51 114003.5 52 98637.5 53 83558.0 54 69399.0 55 56384.5 56 43969.5 57 32454.0 58 23134.0 59 16606.5 60 12836.5 61 10658.5 62 8926.0 63 7087.0 64 5544.0 65 5068.5 66 5745.5 67 5358.5 68 3642.0 69 3259.0 70 4168.0 71 4022.5 72 2430.5 73 1063.5 74 534.0 75 375.0 76 289.0 77 205.0 78 141.5 79 109.0 80 77.0 81 48.5 82 38.0 83 32.5 84 27.5 85 25.5 86 22.0 87 20.0 88 23.5 89 22.5 90 18.5 91 18.5 92 18.0 93 14.5 94 18.0 95 18.0 96 18.5 97 29.5 98 53.0 99 117.5 100 120.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.328104270223417 2 0.006246572676938317 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.3493746793794556E-4 30-31 0.010199050078717872 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.877315101448763E-4 40-41 0.0013128858502414605 42-43 1.243786594965594E-4 44-45 0.0 46-47 0.0021420769135518567 48-49 1.3819851055173267E-5 50-51 0.0 52-53 0.001492543913958713 54-55 0.018034905627001115 56-57 0.011608674886345545 58-59 0.013902770161504307 60-61 1.7965806371725247E-4 62-63 0.011511935928959332 64-65 1.1055880844138614E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9347791477242575E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3617984.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.50611072970563 #Duplication Level Percentage of deduplicated Percentage of total 1 85.26097280662722 61.819415352397336 2 9.076877797716948 13.162582133625433 3 2.539320266581122 5.523487092807493 4 1.1176056394099025 3.241329529727914 5 0.5768301102501339 2.0911853923012256 6 0.3500413585365287 1.522808250121568 7 0.22990072921355828 1.1668445410438826 8 0.15556394286637315 0.9023469173615082 9 0.11749946959463758 0.7667486597799424 >10 0.535729950989598 6.66121026355161 >50 0.027757970333315004 1.373873722764516 >100 0.01120235792018158 1.4151741354608656 >500 6.588444094137151E-4 0.319161437184564 >1k 3.875555103995822E-5 0.03383257187213139 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 2.7639702110346534E-5 0.0 0.0 0.0 0.0 46-47 2.7639702110346534E-5 0.0 0.0 0.0 0.0 48-49 2.7639702110346534E-5 0.0 0.0 0.0 0.0 50-51 5.527940422069307E-5 0.0 0.0 0.0 0.0 52-53 5.527940422069307E-5 0.0 0.0 2.7639702110346534E-5 0.0 54-55 9.673895738621286E-5 0.0 0.0 2.7639702110346534E-5 0.0 56-57 1.3819851055173267E-4 0.0 0.0 2.7639702110346534E-5 0.0 58-59 1.7965806371725247E-4 0.0 0.0 2.7639702110346534E-5 0.0 60-61 2.763970211034654E-4 0.0 0.0 2.7639702110346534E-5 0.0 62-63 3.040367232138119E-4 0.0 0.0 2.7639702110346534E-5 0.0 64-65 3.454962763793317E-4 0.0 0.0 2.7639702110346534E-5 0.0 66-67 4.0077568060002477E-4 0.0 0.0 2.7639702110346534E-5 0.0 68-69 4.698749358758911E-4 0.0 0.0 2.7639702110346534E-5 0.0 70-71 6.080734464276238E-4 0.0 0.0 2.7639702110346534E-5 0.0 72-73 6.771727017034901E-4 0.0 0.0 2.7639702110346534E-5 0.0 74-75 7.73911659089703E-4 0.0 0.0 2.7639702110346534E-5 0.0 76-77 8.706506164759158E-4 0.0 0.0 2.7639702110346534E-5 0.0 78-79 0.0010779483823035148 0.0 0.0 2.7639702110346534E-5 0.0 80-81 0.0012161468928552476 0.0 0.0 2.7639702110346534E-5 0.0 82-83 0.0014649042118483665 0.0 0.0 2.7639702110346534E-5 0.0 84-85 0.0015616431692345792 0.0 0.0 2.7639702110346534E-5 0.0 86-87 0.001658382126620792 5.527940422069307E-5 0.0 2.7639702110346534E-5 0.0 88 0.001658382126620792 5.527940422069307E-5 0.0 2.7639702110346534E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGCA 65 0.0 81.37317 1 TCACCGG 14065 0.0 80.5297 8 CACCGGC 14000 0.0 76.94725 9 CTTCACC 16000 0.0 74.3111 6 CCTTCAC 17630 0.0 70.68881 5 TTCACCG 17305 0.0 67.296684 7 GTGTGGC 5890 0.0 66.783195 7 CGGGGCA 1465 0.0 66.32407 94 CGTAGTG 3135 0.0 66.17946 4 ATGCTGC 95 0.0 64.23656 2 GCTGCAA 115 0.0 61.228825 2 AGGCAGC 70 2.1827873E-11 60.35413 2 GCCTTCA 5550 0.0 59.96416 4 TGTGGCA 6780 0.0 59.88595 8 ACCTTCA 6725 0.0 59.67773 4 GGCCCGA 970 0.0 59.037315 9 CGGGCCC 985 0.0 58.138264 7 GCGTAGT 1995 0.0 57.880287 3 CGCTGCC 110 0.0 57.70097 1 CAGCTGC 110 0.0 57.70097 1 >>END_MODULE