FastQCFastQC Report
Tue 7 Mar 2017
1HL_19A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_19A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3617984
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCA650.081.373171
TCACCGG140650.080.52978
CACCGGC140000.076.947259
CTTCACC160000.074.31116
CCTTCAC176300.070.688815
TTCACCG173050.067.2966847
GTGTGGC58900.066.7831957
CGGGGCA14650.066.3240794
CGTAGTG31350.066.179464
ATGCTGC950.064.236562
GCTGCAA1150.061.2288252
AGGCAGC702.1827873E-1160.354132
GCCTTCA55500.059.964164
TGTGGCA67800.059.885958
ACCTTCA67250.059.677734
GGCCCGA9700.059.0373159
CGGGCCC9850.058.1382647
GCGTAGT19950.057.8802873
CGCTGCC1100.057.700971
CAGCTGC1100.057.700971
Produced by FastQC (version 0.11.5)