FastQCFastQC Report
Mon 6 Mar 2017
1HL_1A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_1A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2260430
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA119560.5289259123264157No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG69440.30719818795538906No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA54140.2395119512659096No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT46550.20593426914348156No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA46470.2055803541803993No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG45900.20305871006843831No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG42840.18952146273054243No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG41400.183150993395062No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG39720.17571877917033485No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37970.16797688935291075No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC37840.16740177753790209No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC35090.1552359506819499No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT35060.15510323257079406No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA35000.15483779634848238No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA34430.15231615223652137No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG33680.14899819945762532No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA31420.13900010175055189No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA31340.13864618678746965No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC28000.12387023707878589No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA27110.11993293311449592No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA26470.11710161340983795No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG25890.11453572992749167No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA23850.10551089836889442No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA23340.10325469047924508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCA302.2700988E-886.999693
GCTGCAA257.8032826E-786.999693
CTGCAGC900.086.999691
GCAGCTC202.6991394E-586.999693
GCAGCGT159.3884213E-486.999693
GCAGCCG159.3884213E-486.999693
TGCAGCC356.6393113E-1086.999692
AGCAGCT550.086.999692
CAGCTGC3750.085.839691
AGCTGCC1600.084.280952
AGCTGCT850.081.8820652
CAGCCCT135550.081.8650741
AGCTGCG800.081.562212
CAGCGTA50750.080.056861
GCTGCCC1250.080.039713
AGCCCTT115350.079.60832
CAGCAGC2600.078.634341
CAGCTCT49950.077.8555761
GCAGCAC455.638867E-1177.333063
CAGCGTG37300.077.2034761
Produced by FastQC (version 0.11.5)