FastQCFastQC Report
Tue 7 Mar 2017
1HL_1A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_1A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2260430
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG88150.080.0836268
CACCGGC88650.077.249349
CTTCACC97550.075.5904856
GGCGCGC2950.073.190386
CCTTCAC109800.070.87625
CGCTGCA1050.070.532151
CGTAGTG19000.069.170784
TTCACCG108250.067.641767
ACCTTCA46650.067.010184
GTGTGGC35750.066.303067
CGGGGCA9300.065.1065994
CGGGCCC6300.064.818197
CAGCTGC751.2732926E-1163.4789351
TGTGGCA38000.062.006668
GCTGCCT601.0057192E-861.715631
GCGTAGT12300.059.9118543
AGGCGCG3550.059.4979975
AACGGGC6800.059.36195
GGGCCCG6800.059.36198
GCCTTCA32150.059.2738654
Produced by FastQC (version 0.11.5)