FastQCFastQC Report
Tue 7 Mar 2017
1HL_20A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_20A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2908938
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA160820.5528478090629639No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG98150.3374083600269239No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA73040.2510881978233981No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT67660.2325934756945662No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA63520.21836147762516767No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG57690.19831979918444465No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG54420.18707858331803565No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG53890.1852566125506972No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50250.1727434548278444No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC46880.16115847089212626No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA44900.15435186311980523No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT43560.14974537099106272No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA42850.1473046176989678No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41130.14139180690685055No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC39510.1358227641840424No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38560.13255696752560556No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG35800.12306896881267321No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34610.11897812878789442No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT33480.1150935496047011No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32340.11117459361457686No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC31650.10880259393634378No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA30350.10433360903532492No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG30030.10323355121353565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC1900.085.999643
GCTGCAT500.085.999643
AGCTGCA1250.085.999642
CAGCTGC5900.085.999641
GCAGCTA258.3596206E-785.999643
GCAGCAA1000.085.999643
AGCAGCA1950.085.999642
GCTGCTC159.829929E-485.999633
GCTGCTA302.4598194E-885.999633
GCTGCAA700.085.999633
GCAGCTC159.829929E-485.999633
GCAGCCT402.1827873E-1185.999633
GCAGCAC450.085.999633
AGCAGCC1400.085.999632
CAGCAGC5150.085.164691
AGCTGCC2250.084.088532
AGCTGCT1600.083.312152
AGCAGCG1250.082.5596542
CTGCAGC950.081.473341
AGCTGCG950.081.473342
Produced by FastQC (version 0.11.5)