FastQCFastQC Report
Tue 7 Mar 2017
1HL_20A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_20A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2908938
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG117350.079.4379658
CACCGGC114350.077.458259
CTTCACC129850.074.212826
ATGCAGC452.6593625E-973.022192
GTGTGGC51350.072.487767
CCTTCAC143700.070.620525
CGTAGTG27050.068.542794
CAGCGTA11650.067.669351
AGCAGCC555.0495146E-967.339051
CGGGGCA12850.066.11613594
TTCACCG147100.065.2867367
TGTGGCA58500.064.270058
CGGGCCC7800.063.7886247
TGTGTGG56050.062.473416
GCCTTCA43200.061.933144
ATGTGTG54450.059.99895
GGCCCGA8600.058.9464049
ACCTTCA55100.058.524424
GCGTAGT18000.057.108873
GCTGCCT1400.056.688491
Produced by FastQC (version 0.11.5)