##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_20A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2908938 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.826163018943683 12.0 12.0 12.0 2.0 32.0 2 14.584936495724557 12.0 12.0 12.0 12.0 22.0 3 13.722473975038312 12.0 12.0 12.0 12.0 22.0 4 13.724273600881146 12.0 12.0 12.0 12.0 22.0 5 22.44176087630606 22.0 22.0 22.0 22.0 27.0 6 26.807454816843812 27.0 22.0 32.0 22.0 32.0 7 34.9757464751741 37.0 37.0 37.0 27.0 41.0 8 37.59097031287707 41.0 37.0 41.0 27.0 41.0 9 38.5494187225716 41.0 41.0 41.0 32.0 41.0 10-11 38.914516397393136 41.0 41.0 41.0 37.0 41.0 12-13 39.09495217842388 41.0 41.0 41.0 37.0 41.0 14-15 39.04979841440415 41.0 41.0 41.0 37.0 41.0 16-17 38.95614292913771 41.0 41.0 41.0 37.0 41.0 18-19 38.88403620152784 41.0 41.0 41.0 37.0 41.0 20-21 38.90401531417995 41.0 41.0 41.0 37.0 41.0 22-23 38.890288998940505 41.0 41.0 41.0 37.0 41.0 24-25 38.901406802070035 41.0 41.0 41.0 37.0 41.0 26-27 38.87102303314818 41.0 41.0 41.0 37.0 41.0 28-29 38.81697977062419 41.0 41.0 41.0 37.0 41.0 30-31 38.68436951904785 41.0 41.0 41.0 32.0 41.0 32-33 38.738980170770226 41.0 41.0 41.0 32.0 41.0 34-35 38.70992437790011 41.0 41.0 41.0 32.0 41.0 36-37 38.63346640595296 41.0 41.0 41.0 32.0 41.0 38-39 38.54232008382441 41.0 41.0 41.0 32.0 41.0 40-41 38.591567609897496 41.0 41.0 41.0 32.0 41.0 42-43 38.33519947829758 41.0 41.0 41.0 32.0 41.0 44-45 38.44612759020646 41.0 41.0 41.0 32.0 41.0 46-47 38.443225328281315 41.0 41.0 41.0 32.0 41.0 48-49 38.40897623118815 41.0 41.0 41.0 32.0 41.0 50-51 38.29887797539858 41.0 41.0 41.0 32.0 41.0 52-53 38.27753805684411 41.0 41.0 41.0 32.0 41.0 54-55 38.120872634617854 41.0 39.0 41.0 32.0 41.0 56-57 37.92649929286908 41.0 37.0 41.0 29.5 41.0 58-59 37.92264702788441 41.0 37.0 41.0 29.5 41.0 60-61 37.927233581465124 41.0 37.0 41.0 29.5 41.0 62-63 37.92388235844146 41.0 37.0 41.0 32.0 41.0 64-65 37.904025627222026 41.0 37.0 41.0 29.5 41.0 66-67 37.78255569558375 41.0 37.0 41.0 27.0 41.0 68-69 37.81089576333356 41.0 37.0 41.0 27.0 41.0 70-71 37.79285670578059 41.0 37.0 41.0 27.0 41.0 72-73 37.64129813010796 41.0 37.0 41.0 27.0 41.0 74-75 37.57414527226088 41.0 37.0 41.0 27.0 41.0 76-77 36.499782738580194 39.0 34.5 41.0 24.5 41.0 78-79 37.02740914381812 41.0 37.0 41.0 27.0 41.0 80-81 37.67843006623036 41.0 37.0 41.0 27.0 41.0 82-83 37.855350646868374 41.0 37.0 41.0 32.0 41.0 84-85 37.779438750499324 41.0 37.0 41.0 27.0 41.0 86-87 37.65419321415582 41.0 37.0 41.0 27.0 41.0 88-89 37.573481799887105 41.0 37.0 41.0 27.0 41.0 90-91 37.393740430356374 41.0 37.0 41.0 27.0 41.0 92-93 37.28796282354591 41.0 37.0 41.0 27.0 41.0 94-95 37.12674763092235 41.0 37.0 41.0 27.0 41.0 96-97 37.070589851004044 41.0 37.0 41.0 27.0 41.0 98-99 36.95777703065517 41.0 37.0 41.0 27.0 41.0 100 36.828635055130086 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3080020518576525 1101 2 -0.6179195665345087 1101 3 0.009690417716788247 1101 4 0.09203280406972425 1101 5 -1.1686735419619438 1101 6 -0.6920544231550849 1101 7 -0.3314193479474312 1101 8 -0.046365815403163424 1101 9 -0.10218869668211994 1101 10-11 -0.12103990110475138 1101 12-13 -0.043842736680851147 1101 14-15 0.1462357309249711 1101 16-17 -0.05347071311209106 1101 18-19 0.008726281575476946 1101 20-21 0.03913068363096528 1101 22-23 0.11635093047757294 1101 24-25 0.11904777814789469 1101 26-27 0.06939004087718814 1101 28-29 -0.001523153455913473 1101 30-31 0.11920151993517436 1101 32-33 -0.03992059319723751 1101 34-35 0.09503759045872329 1101 36-37 0.09203318840739882 1101 38-39 0.1027712936130385 1101 40-41 0.08856905845363627 1101 42-43 0.19104543158926646 1101 44-45 0.1191919554291303 1101 46-47 0.10105644722813167 1101 48-49 0.15290207850264892 1101 50-51 0.13048849009874175 1101 52-53 0.14637438428034244 1101 54-55 0.21867649156011737 1101 56-57 0.27760427109384267 1101 58-59 0.11305643610918992 1101 60-61 0.0014035281473354644 1101 62-63 0.01180861735755201 1101 64-65 0.07867734188465647 1101 66-67 -0.02010647422687839 1101 68-69 0.08915938074115104 1101 70-71 0.09342227920764401 1101 72-73 0.042058022493748126 1101 74-75 0.19733708562965546 1101 76-77 -0.18024240111591183 1101 78-79 -0.12483681469526431 1101 80-81 -0.011181582011545288 1101 82-83 -0.11082296053084661 1101 84-85 -0.07332717333210326 1101 86-87 -0.12159395800203043 1101 88-89 -0.23361385817547387 1101 90-91 -0.025356943255722797 1101 92-93 0.17807111120957586 1101 94-95 0.008419804497592054 1101 96-97 -0.06814559380582352 1101 98-99 -0.3466600606659114 1101 100 -0.49225989422679817 1102 1 1.4722076350771598 1102 2 0.8222492123450724 1102 3 -0.17278283116405646 1102 4 -0.004146778387493555 1102 5 -0.7740460280562829 1102 6 -0.30533549195989096 1102 7 -0.10439320052140744 1102 8 -0.31006780310953275 1102 9 -0.2586877538760177 1102 10-11 -0.5104438894274921 1102 12-13 -0.40029504762537726 1102 14-15 -0.2669267309060501 1102 16-17 -0.355121303062063 1102 18-19 -0.3498528786343016 1102 20-21 -0.17340938056180732 1102 22-23 -0.13998446980857437 1102 24-25 -0.283003367412924 1102 26-27 -0.21442626948067556 1102 28-29 -0.44592468962714094 1102 30-31 -0.39360340964746854 1102 32-33 -0.37321587202120554 1102 34-35 -0.2020073530427169 1102 36-37 -0.3929683060014355 1102 38-39 -0.37607355162219847 1102 40-41 -0.30695780285096674 1102 42-43 -0.30644056892983684 1102 44-45 -0.17431609538888182 1102 46-47 -0.6640262361386817 1102 48-49 -0.5931861506146276 1102 50-51 -0.4185599445098802 1102 52-53 -0.44394870676060094 1102 54-55 -0.435084517414289 1102 56-57 -0.2841539943530975 1102 58-59 -0.34856600401199955 1102 60-61 -0.2660858214688204 1102 62-63 -0.5626628565468863 1102 64-65 -0.717671887124979 1102 66-67 -0.6152661658097145 1102 68-69 -0.5151249115132757 1102 70-71 -0.6242656518985257 1102 72-73 -0.32370841108716775 1102 74-75 -0.6113741545015046 1102 76-77 -0.48640624086544193 1102 78-79 -0.6528680515178635 1102 80-81 -0.5722977995824579 1102 82-83 -0.5963908441461996 1102 84-85 -0.4782633310756239 1102 86-87 -0.3739123933327022 1102 88-89 -0.5432445613042205 1102 90-91 -0.6610374817292808 1102 92-93 -0.49762415668590876 1102 94-95 -0.4843000000748603 1102 96-97 -0.42082502907390307 1102 98-99 -0.6950328063115165 1102 100 -0.8709716162316283 1103 1 1.4472529020911722 1103 2 0.7681439828124912 1103 3 0.3874514293234874 1103 4 0.8956273759575666 1103 5 3.005273433232844 1103 6 -0.09482826213334761 1103 7 -0.5238482127070654 1103 8 -0.37684080492083183 1103 9 -0.4034679319112513 1103 10-11 -0.1818297607744057 1103 12-13 -0.3945469836117397 1103 14-15 -0.4262589294177985 1103 16-17 -0.34019678330258785 1103 18-19 -0.36834919652356035 1103 20-21 -0.2577276812379452 1103 22-23 -0.1550374804573167 1103 24-25 -0.255356229807731 1103 26-27 -0.1751006772961503 1103 28-29 -0.2740534427807617 1103 30-31 -0.1482909188725614 1103 32-33 -0.3164710636280006 1103 34-35 -0.23120702415575778 1103 36-37 -0.27182462522065975 1103 38-39 -0.14235266366470256 1103 40-41 -0.49312199090196884 1103 42-43 -0.13591707809184328 1103 44-45 -0.49068249422894894 1103 46-47 -0.5633261239305583 1103 48-49 -0.34846429843166504 1103 50-51 -0.19232384506445044 1103 52-53 -0.28072627955372553 1103 54-55 -0.22807303216215047 1103 56-57 -0.3517421442303359 1103 58-59 -0.4593559914798675 1103 60-61 -0.23301914945743363 1103 62-63 -0.4617513636194843 1103 64-65 -0.5200231461026306 1103 66-67 -0.5025065543959997 1103 68-69 -0.3133746387614593 1103 70-71 -0.6341978267318993 1103 72-73 -0.44320120922720463 1103 74-75 -0.47879812502846164 1103 76-77 -0.5595474655591843 1103 78-79 -0.46947076794266707 1103 80-81 -0.3356611628058488 1103 82-83 -0.3835560958593831 1103 84-85 -0.5090161571760277 1103 86-87 -0.33656124156026124 1103 88-89 -0.3178317668433408 1103 90-91 -0.7476369229570494 1103 92-93 -0.5419201089587702 1103 94-95 -0.6174051416680939 1103 96-97 -0.3163461660582385 1103 98-99 -0.38124065329829193 1103 100 -0.6033529050993423 1104 1 1.5926573308265155 1104 2 -0.4884934993436332 1104 3 0.06388771608909494 1104 4 0.019801596243784303 1104 5 -1.09764985364518 1104 6 -0.5787730131606459 1104 7 -0.4711411876367748 1104 8 -0.0826663397032803 1104 9 -0.10728879792564783 1104 10-11 -0.1668211222346443 1104 12-13 -0.130849826762109 1104 14-15 -0.25396543636411195 1104 16-17 -0.056807775192851295 1104 18-19 -0.14393824068216787 1104 20-21 -0.11641360578779114 1104 22-23 -0.0046074061551948375 1104 24-25 -0.1856820575657352 1104 26-27 -0.1242012768755032 1104 28-29 -0.12194637387403873 1104 30-31 -0.1024838921065907 1104 32-33 -0.09053007198266982 1104 34-35 -0.1467768380644685 1104 36-37 -0.24148658229739084 1104 38-39 -0.16731744693500872 1104 40-41 -0.09641314580345295 1104 42-43 -0.07697262537835314 1104 44-45 -0.12705570803579036 1104 46-47 -0.14111788582212625 1104 48-49 -0.2919567554886058 1104 50-51 -0.2784486105741024 1104 52-53 -0.05885318915459692 1104 54-55 -0.08524964586712969 1104 56-57 -0.22421770290929288 1104 58-59 -0.17229081525177037 1104 60-61 -0.1360542883115201 1104 62-63 -0.16460694896454697 1104 64-65 -0.2648718947233277 1104 66-67 -0.6551559263983364 1104 68-69 -0.4381773591321405 1104 70-71 -0.20841626026412996 1104 72-73 -0.2325159855577965 1104 74-75 -0.30347313568925216 1104 76-77 -0.4825272373711087 1104 78-79 -0.20582087028000018 1104 80-81 -0.35490648540239533 1104 82-83 -0.38508409056886705 1104 84-85 -0.22249830827605166 1104 86-87 -0.24590414731257226 1104 88-89 -0.33553684816368445 1104 90-91 -0.2749776971787199 1104 92-93 -0.24949705978975345 1104 94-95 -0.2560301536791485 1104 96-97 -0.2925562681569289 1104 98-99 -0.4349380802004248 1104 100 -0.33583105125803314 1105 1 1.575187870734105 1105 2 0.49275003927695415 1105 3 -0.08071004326276388 1105 4 -0.15152781279054395 1105 5 -1.0634915735852601 1105 6 0.4496844841493086 1105 7 0.1288630320395754 1105 8 0.03847212897616714 1105 9 0.019577881400046238 1105 10-11 0.01700696815082381 1105 12-13 0.05117329706428819 1105 14-15 -0.057191378934319914 1105 16-17 -0.01770930904520185 1105 18-19 -0.05758994859617417 1105 20-21 -0.02234436089887737 1105 22-23 0.061462176307827576 1105 24-25 -0.08922869553860124 1105 26-27 -0.099821041839526 1105 28-29 0.1446654370000644 1105 30-31 -0.06630966167006136 1105 32-33 -0.11525421064123265 1105 34-35 0.0041630948426742975 1105 36-37 -0.17974216809395926 1105 38-39 -0.06184980130343121 1105 40-41 -0.06846095473650138 1105 42-43 -0.036376174126154126 1105 44-45 0.07442434344425664 1105 46-47 -0.2720512782309328 1105 48-49 -0.2178286461145973 1105 50-51 -0.06195284079964836 1105 52-53 -0.10392460717682894 1105 54-55 -0.11141480399950154 1105 56-57 -0.08903826117246894 1105 58-59 -0.0153916444181732 1105 60-61 -0.288546007196679 1105 62-63 -0.27671003565779984 1105 64-65 -0.23724250889722498 1105 66-67 -0.0736359989767692 1105 68-69 -0.20288609597202623 1105 70-71 -0.3129386983931255 1105 72-73 -0.2975770653729768 1105 74-75 -0.30286339245655824 1105 76-77 -0.2412387591973797 1105 78-79 -0.3609308983408468 1105 80-81 -0.5294829353886854 1105 82-83 -0.39475875352221834 1105 84-85 -0.16274452639111558 1105 86-87 -0.17402618035263373 1105 88-89 -0.2544578769207817 1105 90-91 -0.29645268504715006 1105 92-93 -0.13692347918338044 1105 94-95 -0.24786824469757107 1105 96-97 -0.11586470539978677 1105 98-99 -0.21077515849651718 1105 100 -0.40647898256170123 1106 1 1.1178946882367224 1106 2 -0.4841485128841416 1106 3 -0.12511008591251382 1106 4 -0.19632732141735687 1106 5 -1.1925791542481043 1106 6 -0.7036362645039951 1106 7 -0.24107369915736854 1106 8 -0.21396964435241728 1106 9 -0.23836255612032176 1106 10-11 -0.09455862311138219 1106 12-13 -0.1044779451097071 1106 14-15 -0.10429044499488072 1106 16-17 -0.10877636376453381 1106 18-19 0.03237279294183537 1106 20-21 -0.05639312878661684 1106 22-23 -0.10865138616279779 1106 24-25 -0.2735810425513918 1106 26-27 -0.1581818947744722 1106 28-29 -0.20703387024182973 1106 30-31 -0.1051687658150513 1106 32-33 -0.1618866824195635 1106 34-35 -0.22599268187085642 1106 36-37 -0.33544304316473017 1106 38-39 0.08809943948595134 1106 40-41 -0.008133632915601652 1106 42-43 0.02129101965538638 1106 44-45 -0.08593774393066411 1106 46-47 -0.03639984082920478 1106 48-49 -0.16929150253660907 1106 50-51 -0.22856506896380324 1106 52-53 -0.08277543666943643 1106 54-55 -0.336283288086193 1106 56-57 -0.1631098406699607 1106 58-59 -0.22238500216999313 1106 60-61 -0.1590870433153171 1106 62-63 -0.32136394944823365 1106 64-65 -0.5232407758756494 1106 66-67 -0.3051916336245881 1106 68-69 -0.39788398601075414 1106 70-71 -0.4867948515958389 1106 72-73 -0.3488905725791298 1106 74-75 -0.4749570897544544 1106 76-77 -0.30756843276159884 1106 78-79 -0.3055752009198116 1106 80-81 -0.07755994221714957 1106 82-83 -0.24986733768565017 1106 84-85 -0.4479197582991432 1106 86-87 -0.341510701046019 1106 88-89 -0.5100805107231139 1106 90-91 -0.6565424297343228 1106 92-93 -0.5290771264128793 1106 94-95 -0.38879180703303007 1106 96-97 -0.3089037103991217 1106 98-99 -0.655978563268178 1106 100 -0.6751904344703519 1107 1 1.3001302274715343 1107 2 -0.6095491983371062 1107 3 -0.12428563181293129 1107 4 -0.1390939307829413 1107 5 -1.106761392373265 1107 6 -0.24082849936328188 1107 7 -0.4185412469251233 1107 8 -0.49035312624720717 1107 9 -0.22726649915571784 1107 10-11 -0.18745878035090158 1107 12-13 -0.362827862595843 1107 14-15 -0.3767687220744307 1107 16-17 -0.26310177397228074 1107 18-19 -0.14209051710142973 1107 20-21 -0.11543864519378388 1107 22-23 -0.10200951092217281 1107 24-25 -0.2356998104884056 1107 26-27 -0.2963508947831457 1107 28-29 -0.11726207518164955 1107 30-31 -0.23916689512565625 1107 32-33 -0.19580581929217544 1107 34-35 -0.27588561692289204 1107 36-37 -0.15838962386304445 1107 38-39 -0.2547847078284846 1107 40-41 -0.18694167916169135 1107 42-43 -0.28568853190547117 1107 44-45 -0.1952811210011376 1107 46-47 -0.4283533817758922 1107 48-49 -0.4008239969042364 1107 50-51 -0.34742062323653045 1107 52-53 -0.2480931625694751 1107 54-55 -0.40956470819163826 1107 56-57 -0.30749852544814615 1107 58-59 -0.513208523815031 1107 60-61 -0.12158046631639507 1107 62-63 -0.3101285566985297 1107 64-65 -0.3903300285080036 1107 66-67 -0.36040039620264963 1107 68-69 -0.7031785024631034 1107 70-71 -0.4724384741619687 1107 72-73 -0.4327291122184107 1107 74-75 -0.19227832277880452 1107 76-77 -0.31236669477584655 1107 78-79 -0.6267637321244024 1107 80-81 -0.37303927176864704 1107 82-83 -0.29985884215769687 1107 84-85 -0.3928892725116171 1107 86-87 -0.38176117385219754 1107 88-89 -0.2906596577842748 1107 90-91 -0.07002768470740506 1107 92-93 -0.30946308957985735 1107 94-95 -0.14228352986373238 1107 96-97 -0.15336770647338227 1107 98-99 -0.5623671242261423 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0.19921469074756715 2223 12-13 0.2257535491044962 2223 14-15 0.17322602768446416 2223 16-17 0.29365944774689723 2223 18-19 0.06746696513351225 2223 20-21 0.14391603156288824 2223 22-23 0.12933087861588888 2223 24-25 0.242710288231045 2223 26-27 0.09576015557232864 2223 28-29 0.09192356469297636 2223 30-31 0.05673855817449436 2223 32-33 0.22822699531137403 2223 34-35 0.2329233032367526 2223 36-37 0.14953284081491347 2223 38-39 0.14593405316234254 2223 40-41 0.2503746183324793 2223 42-43 0.2818141312494973 2223 44-45 0.31742505909944185 2223 46-47 0.14894083189161478 2223 48-49 0.05014180881238417 2223 50-51 0.28556253989651026 2223 52-53 0.2638544173527677 2223 54-55 0.3511665907329231 2223 56-57 0.4231464308749153 2223 58-59 0.4078842303451111 2223 60-61 0.3084099549766748 2223 62-63 0.38695102651584534 2223 64-65 0.25364731849145983 2223 66-67 0.3111756187867911 2223 68-69 0.2785637095052351 2223 70-71 0.3574714633213816 2223 72-73 0.35723976518756473 2223 74-75 0.43798314221148615 2223 76-77 0.2929471756750459 2223 78-79 0.4206897219525345 2223 80-81 0.30294493216504037 2223 82-83 0.21977802909236743 2223 84-85 0.1847390474082502 2223 86-87 0.34911377882851014 2223 88-89 0.27124739683941357 2223 90-91 0.4099245977603445 2223 92-93 0.45282851537410806 2223 94-95 0.5219250693749231 2223 96-97 0.4643614180757609 2223 98-99 0.5683162244850308 2223 100 0.5951555385236205 2224 1 1.2839789659062255 2224 2 -0.9356205107462348 2224 3 -0.10900585464962376 2224 4 -0.3801689580345968 2224 5 -1.0807021386152584 2224 6 0.6022939851138212 2224 7 0.19451309570035136 2224 8 0.11990947407074515 2224 9 0.11227624158119198 2224 10-11 0.14695589182584 2224 12-13 0.05822649738450991 2224 14-15 0.13202661269377103 2224 16-17 0.12457518224464081 2224 18-19 0.10520631033627836 2224 20-21 0.13854931231308143 2224 22-23 0.29963692882841286 2224 24-25 0.2974901306216893 2224 26-27 0.09704599133201697 2224 28-29 0.13717045157853391 2224 30-31 0.1336613881862192 2224 32-33 0.21248327654537746 2224 34-35 0.2203478499333258 2224 36-37 0.11884976190577845 2224 38-39 0.07820641514911841 2224 40-41 0.17933558483510126 2224 42-43 0.4024995854458524 2224 44-45 0.3734816913156891 2224 46-47 0.17927082756707335 2224 48-49 0.20153397806822682 2224 50-51 0.17834516843485915 2224 52-53 0.15152200787192527 2224 54-55 0.26752219398875354 2224 56-57 0.40453909036202873 2224 58-59 0.36395877934693743 2224 60-61 0.2797072061215715 2224 62-63 0.4491753808550101 2224 64-65 0.44369086736898566 2224 66-67 0.18314749235148042 2224 68-69 0.2930094826320442 2224 70-71 0.3330489003106152 2224 72-73 0.5259490621317298 2224 74-75 0.30831668039145654 2224 76-77 0.23914633679597586 2224 78-79 0.2891482714399274 2224 80-81 0.18468860307512358 2224 82-83 0.22234590715550695 2224 84-85 0.018616012199721865 2224 86-87 0.23784441250495547 2224 88-89 0.28592063143783975 2224 90-91 0.4592247926272748 2224 92-93 0.2027847447252995 2224 94-95 0.4607865189304192 2224 96-97 0.29424037180551466 2224 98-99 0.31173180578550586 2224 100 0.663419366984698 2225 1 1.5917886994736623 2225 2 1.7007011778639303 2225 3 0.9026742493700848 2225 4 1.0785887612566665 2225 5 3.2190238071960167 2225 6 0.13898451911408571 2225 7 0.34408535593242107 2225 8 0.5470846225597654 2225 9 0.23774691748815968 2225 10-11 0.18091885691820409 2225 12-13 0.2557042111327732 2225 14-15 0.24050578147960522 2225 16-17 0.13327268037609485 2225 18-19 0.15525923419520637 2225 20-21 0.1743942439322197 2225 22-23 0.26154958824495367 2225 24-25 0.26044881020826693 2225 26-27 0.2576219916150677 2225 28-29 0.12624123244869878 2225 30-31 0.25866820637138943 2225 32-33 0.18737149516366003 2225 34-35 0.33744881656770076 2225 36-37 0.27428956801870186 2225 38-39 0.29272686454491037 2225 40-41 0.18138170377667961 2225 42-43 0.4297061344416946 2225 44-45 0.23374803542691325 2225 46-47 0.26267543857670717 2225 48-49 0.3020183272005852 2225 50-51 0.2630101486142138 2225 52-53 0.2966965755252744 2225 54-55 0.3180281401833298 2225 56-57 0.3993136332487879 2225 58-59 0.5050099297406305 2225 60-61 0.44430674646532253 2225 62-63 0.4225806257715945 2225 64-65 0.4924909404894109 2225 66-67 0.6005075500222645 2225 68-69 0.7011337372385569 2225 70-71 0.49732234712803347 2225 72-73 0.566496256503811 2225 74-75 0.4422895226385535 2225 76-77 0.6322460385648725 2225 78-79 0.536227655754125 2225 80-81 0.38216963377833224 2225 82-83 0.3569281108814053 2225 84-85 0.44607663994450064 2225 86-87 0.38976072794294225 2225 88-89 0.5657130785102922 2225 90-91 0.4758209924288437 2225 92-93 0.5670342357569922 2225 94-95 0.6517884309706687 2225 96-97 0.4898044236797432 2225 98-99 0.6274580788696653 2225 100 0.6863368721038654 2226 1 1.2802144494631058 2226 2 1.4812902354296078 2226 3 0.9581753572143317 2226 4 1.010615793989741 2226 5 3.191364369852 2226 6 -0.6440433514470385 2226 7 0.05462398825035564 2226 8 0.16421942842589488 2226 9 0.124048095558841 2226 10-11 0.14360172900164514 2226 12-13 0.044970678231912586 2226 14-15 0.03606240376582548 2226 16-17 -0.05628824883521588 2226 18-19 -0.0601443566869051 2226 20-21 -0.10301306717311576 2226 22-23 -0.09828444624156418 2226 24-25 -0.08422720368589864 2226 26-27 -0.029716359797127723 2226 28-29 0.05716686576447216 2226 30-31 0.01192895757201029 2226 32-33 0.011231815671784773 2226 34-35 0.008403073823139096 2226 36-37 0.15550088514217464 2226 38-39 0.03533519882274305 2226 40-41 0.09730288126954889 2226 42-43 0.2378121763597676 2226 44-45 0.10225170120241245 2226 46-47 0.12070336469672327 2226 48-49 0.032961961596427614 2226 50-51 -0.05971765949872321 2226 52-53 0.07852304066873472 2226 54-55 -0.01999236326910392 2226 56-57 0.13460686195362115 2226 58-59 0.07879235668624318 2226 60-61 0.16660830678116412 2226 62-63 0.1399183327006739 2226 64-65 0.19514821103675217 2226 66-67 0.08883440518913233 2226 68-69 0.12227700583467538 2226 70-71 0.2823247806073752 2226 72-73 0.2615135130947692 2226 74-75 0.1521838940786182 2226 76-77 0.30359965311507864 2226 78-79 0.37170200643738127 2226 80-81 0.20958839001481522 2226 82-83 0.13014717069166437 2226 84-85 -0.0909798056644604 2226 86-87 0.12741920967407339 2226 88-89 0.17897387415803223 2226 90-91 0.22469703610257596 2226 92-93 0.11601561908444324 2226 94-95 0.2907092106618947 2226 96-97 0.2507116336678976 2226 98-99 0.25891863316959274 2226 100 0.34526485222711756 2227 1 1.1327618137247146 2227 2 1.8406872622141783 2227 3 0.5025279566419023 2227 4 -0.1450227958688597 2227 5 3.327423150662799 2227 6 0.08837793950943151 2227 7 0.44205777440098615 2227 8 0.3659180272554039 2227 9 0.38102057409273726 2227 10-11 0.3276321766927026 2227 12-13 0.3218920813247408 2227 14-15 0.27074444379316986 2227 16-17 0.30969011418755343 2227 18-19 0.3673087596358755 2227 20-21 0.4090656205111074 2227 22-23 0.43206045315610453 2227 24-25 0.5544307377041662 2227 26-27 0.5297799877615006 2227 28-29 0.5069631513811146 2227 30-31 0.5047655802385123 2227 32-33 0.39930037370501736 2227 34-35 0.4627088429717574 2227 36-37 0.37005912129066587 2227 38-39 0.41212804559108207 2227 40-41 0.3612985666896833 2227 42-43 0.5914762318512459 2227 44-45 0.4258819021934883 2227 46-47 0.3109527078980534 2227 48-49 0.40335235631640387 2227 50-51 0.43554391190544095 2227 52-53 0.39478094361205507 2227 54-55 0.486950078740378 2227 56-57 0.5798696348187562 2227 58-59 0.5246071341222702 2227 60-61 0.4833294396787622 2227 62-63 0.401400962244864 2227 64-65 0.43218778449140416 2227 66-67 0.40979803029058814 2227 68-69 0.47372369834610595 2227 70-71 0.5846504085709796 2227 72-73 0.5237223590514475 2227 74-75 0.4413375048693382 2227 76-77 0.5565919584249954 2227 78-79 0.4765114547264986 2227 80-81 0.3041186721712563 2227 82-83 0.3969275756641153 2227 84-85 0.4839593203127279 2227 86-87 0.5605458917194426 2227 88-89 0.552192374521141 2227 90-91 0.4282078385717085 2227 92-93 0.34444538968548244 2227 94-95 0.4588229703269775 2227 96-97 0.44280989181647357 2227 98-99 0.6084710222080005 2227 100 0.49365329257057056 2228 1 1.383926869522666 2228 2 1.839901831339155 2228 3 -0.13354161782518048 2228 4 2.8559312122311553E-4 2228 5 3.1208930724844777 2228 6 0.06334887562536906 2228 7 0.2377889723036759 2228 8 0.2604340724020844 2228 9 0.16566292946755823 2228 10-11 0.13245451634713135 2228 12-13 0.08228308175775823 2228 14-15 0.08697913818701863 2228 16-17 0.18795921280707262 2228 18-19 0.1389666483035512 2228 20-21 0.0567795754114897 2228 22-23 0.0992041146406919 2228 24-25 0.14372084351082037 2228 26-27 0.07498903592517792 2228 28-29 0.12760430419360347 2228 30-31 0.2106617245083342 2228 32-33 0.09460871856977349 2228 34-35 0.12352434378836818 2228 36-37 0.162195134384703 2228 38-39 0.21887248462282116 2228 40-41 0.13682124593265854 2228 42-43 0.32313267026640347 2228 44-45 0.4214075201834433 2228 46-47 0.3029256484806524 2228 48-49 0.2308633523486634 2228 50-51 0.2904837278969268 2228 52-53 0.22780966508355505 2228 54-55 0.3159086062251646 2228 56-57 0.4691405019815065 2228 58-59 0.42586560324395606 2228 60-61 0.3952393957652376 2228 62-63 0.30847912114782616 2228 64-65 0.27188627022774625 2228 66-67 0.4012997858903873 2228 68-69 0.339145045239448 2228 70-71 0.49828544443604983 2228 72-73 0.3559051244035345 2228 74-75 0.3276108648190714 2228 76-77 0.2268794761339663 2228 78-79 0.34788898575543925 2228 80-81 0.3629964973690534 2228 82-83 0.24395299891427413 2228 84-85 0.14605460278544058 2228 86-87 0.2509369833448005 2228 88-89 0.374237492376956 2228 90-91 0.5268531762305031 2228 92-93 0.35990602283101936 2228 94-95 0.47974131846289225 2228 96-97 0.37551736617670883 2228 98-99 0.6285388546070507 2228 100 0.6796274386571852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 19.0 13 28.0 14 141.0 15 477.0 16 1861.0 17 4678.0 18 7629.0 19 9753.0 20 11649.0 21 13269.0 22 15443.0 23 17872.0 24 19989.0 25 22518.0 26 25676.0 27 29042.0 28 33098.0 29 37421.0 30 42920.0 31 49480.0 32 57900.0 33 69396.0 34 85020.0 35 110080.0 36 154901.0 37 256400.0 38 632741.0 39 1198195.0 40 1341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.124436255882564 19.089459995434627 20.60924863959248 34.176855109090326 2 19.136940798543428 51.91979636585663 11.418597567474094 17.52466526812585 3 40.48243723310706 15.341887657970021 24.642429642708095 19.53324546621482 4 17.708627684742677 16.325579988298138 21.72844522640221 44.237347100556974 5 17.823102451822624 35.29954918255391 26.06655762343508 20.810790742188388 6 17.413777811696228 27.66325717495526 30.74190649646022 24.181058516888292 7 20.334328198125913 26.40901937408085 36.35381709751119 16.90283533028205 8 21.14431452303212 27.73067009334678 34.082850854848054 17.042164528773043 9 20.8205881321637 28.08461369750748 27.717710037133823 23.377088133195002 10-11 19.577488416734905 30.241466129563438 28.633559738983784 21.54748571471788 12-13 24.39484787919165 26.4471604413707 27.223182481029156 21.93480919840849 14-15 21.697299839322802 27.267648880794297 28.677012022944453 22.35803925693844 16-17 21.02189871355113 29.462951771402484 28.334670591122947 21.180478923923438 18-19 21.454582393987085 29.619314677727747 29.772789932270815 19.153312996014353 20-21 22.973023144529034 28.928048655557458 29.68760420469601 18.4113239952175 22-23 22.005866058334693 30.427135263797307 28.585294702052778 18.98170397581523 24-25 22.277150630230004 28.155979948696054 26.68276188767172 22.884107533402222 26-27 20.3122926648832 26.313279966778254 30.069324268856885 23.305103099481666 28-29 19.759691460115704 27.91794018894252 30.650167570379043 21.67220078056273 30-31 20.160459512697745 30.05394433458999 28.070255357603614 21.715340795108652 32-33 20.669983340999362 30.032231694178424 28.738202739281483 20.55958222554073 34-35 21.48065720204418 29.22948168713118 29.768252193755934 19.521608917068704 36-37 21.92963548896539 28.066634627482607 28.686465644850458 21.317264238701547 38-39 21.460299060370012 29.35651864219978 28.960167567863028 20.223014729567183 40-41 20.95531481117655 29.757404402256597 29.865022605666862 19.422258180899995 42-43 20.549063394496027 29.880311887286602 29.34099088824761 20.22963382996976 44-45 20.621322970788654 30.142099969129628 28.705957294380287 20.530619765701434 46-47 19.842843158572336 27.861021992160207 29.01251427741457 23.28362057185289 48-49 20.990554281512456 28.115923445574794 30.274426706387935 20.619095566524816 50-51 21.894107059002287 30.601133472078125 28.19202403076312 19.312735438156466 52-53 20.358860984469032 30.35795686201441 28.186481616062164 21.096700537454392 54-55 22.47499323932514 28.16126663671855 28.168246507013308 21.195493616942997 56-57 21.549752724636033 28.67546735121147 29.254904293286167 20.519875630866334 58-59 21.18116392482183 27.8064427623273 28.996264058773857 22.01612925407701 60-61 19.556682201593784 30.439295699551277 29.555602766516476 20.448419332338467 62-63 20.36565028571586 31.135920158744597 27.313407973639293 21.18502158190026 64-65 19.795801556239656 30.252824487312367 28.80409840714901 21.147275549298968 66-67 20.292818203756834 30.25380740325163 29.437048847380044 20.01632554561149 68-69 20.892830991929014 29.987971555254873 29.24319803309661 19.8759994197195 70-71 19.690725618765338 30.870853899258076 29.130545236784013 20.307875245192577 72-73 20.00468899646538 30.911813864716265 28.463566428710408 20.61993071010795 74-75 20.0820540004634 29.174169404779338 30.307675859712376 20.436100735044885 76-77 19.555195057440205 30.592333009503815 28.51800210248551 21.33446983057047 78-79 19.546171145620843 29.413088212949194 28.469221413450548 22.57151922797942 80-81 21.147217700232748 29.20438023076155 28.10992413542627 21.538477933579436 82-83 20.36537045478453 27.871150914869965 30.042922881133936 21.720555749211567 84-85 21.43916439607857 28.748653288588482 28.105531984524934 21.706650330808014 86-87 21.86148346922485 28.519686566025126 28.747725114801348 20.871104849948676 88-89 21.82913489390286 28.087638856517394 28.903452050198386 21.179774199381356 90-91 22.442812462830076 29.081317649258935 27.89775856343449 20.57811132447649 92-93 21.780887045375323 29.085150663231733 29.12011187588048 20.013850415512465 94-95 22.586782530256748 29.499958404063616 28.017957068868434 19.895301996811206 96-97 21.749157252578087 29.525637878841003 28.548906851916406 20.1762980166645 98-99 23.10686924231455 30.28364647166767 27.413372165374444 19.196112120643342 100 22.41797521982249 31.426451852875516 26.594310363438478 19.56126256386351 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 4.5 2 11.5 3 20.5 4 26.5 5 30.5 6 32.0 7 40.0 8 47.0 9 48.5 10 69.5 11 98.0 12 114.0 13 128.5 14 143.5 15 183.0 16 248.5 17 316.0 18 473.0 19 746.0 20 1188.0 21 1911.5 22 2878.0 23 4410.5 24 7011.0 25 10636.5 26 15213.0 27 20342.5 28 26392.0 29 33388.0 30 40823.5 31 49500.0 32 59511.5 33 70902.5 34 84322.5 35 99075.5 36 112738.5 37 122919.5 38 129835.0 39 136202.0 40 140947.0 41 141604.5 42 140302.5 43 139302.5 44 138650.0 45 133902.5 46 123166.0 47 112436.5 48 105383.5 49 102121.5 50 99533.0 51 92124.0 52 79953.0 53 67461.0 54 56590.5 55 46448.0 56 35898.0 57 26314.5 58 18909.0 59 13653.0 60 10554.5 61 8696.0 62 7321.5 63 5715.0 64 4194.0 65 3664.0 66 4044.0 67 3747.5 68 2572.0 69 2348.5 70 2918.0 71 2660.0 72 1563.5 73 672.5 74 310.0 75 193.5 76 137.0 77 102.0 78 81.0 79 66.5 80 54.0 81 44.5 82 33.0 83 24.0 84 22.0 85 19.5 86 21.0 87 17.0 88 14.0 89 15.5 90 15.0 91 18.0 92 18.5 93 14.0 94 10.5 95 14.5 96 21.0 97 23.5 98 42.0 99 104.0 100 111.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.297765713810332 2 0.006600346930735547 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.7814487624005736E-4 30-31 0.01002083922036152 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.281737871346862E-4 40-41 0.0014953911014947724 42-43 8.594201732728576E-5 44-45 0.0 46-47 0.0020797968193203157 48-49 3.4376806930914304E-5 50-51 0.0 52-53 0.0013750722772365722 54-55 0.01974947558181027 56-57 0.011636549146114492 58-59 0.014919534208016809 60-61 3.093912623782288E-4 62-63 0.012599099740180093 64-65 1.0313042079274292E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.546956311891144E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2908938.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.6509741547109 #Duplication Level Percentage of deduplicated Percentage of total 1 85.27713613018281 61.95467012981675 2 9.029414321596462 13.119914930209623 3 2.561514292771876 5.582895260432765 4 1.1059692206118596 3.213989650503119 5 0.5721446641344097 2.0783433603392374 6 0.3722487498615962 1.6226540583190963 7 0.2318273564139329 1.1789738295428516 8 0.15766949623965582 0.9163873997034819 9 0.11855972523220507 0.77521315802711 >10 0.5365056375333379 6.610484287840164 >50 0.02551892009872355 1.2457032076542756 >100 0.01086522167454221 1.4018795031880278 >500 6.262636486504036E-4 0.2988912244234842 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 1.7188403465457152E-5 0.0 0.0 50-51 3.4376806930914304E-5 0.0 3.4376806930914304E-5 0.0 0.0 52-53 3.4376806930914304E-5 0.0 3.4376806930914304E-5 0.0 0.0 54-55 3.4376806930914304E-5 0.0 3.4376806930914304E-5 0.0 0.0 56-57 1.3750722772365722E-4 0.0 2.4063764851640015E-4 0.0 0.0 58-59 1.5469563118911437E-4 0.0 4.46898490101886E-4 0.0 0.0 60-61 1.7188403465457153E-4 0.0 4.46898490101886E-4 0.0 0.0 62-63 2.0626084158548584E-4 0.0 4.46898490101886E-4 0.0 0.0 64-65 3.4376806930914305E-4 0.0 4.46898490101886E-4 0.0 0.0 66-67 4.125216831709717E-4 0.0 4.46898490101886E-4 0.0 0.0 68-69 4.984637004982575E-4 0.0 4.46898490101886E-4 0.0 0.0 70-71 5.328405074291717E-4 0.0 4.46898490101886E-4 0.0 0.0 72-73 6.015941212910004E-4 0.0 4.46898490101886E-4 0.0 0.0 74-75 6.875361386182862E-4 0.0 4.46898490101886E-4 0.0 0.0 76-77 8.250433663419433E-4 0.0 4.46898490101886E-4 0.0 0.0 78-79 9.281737871346862E-4 0.0 4.46898490101886E-4 0.0 0.0 80-81 9.96927400996515E-4 0.0 4.46898490101886E-4 0.0 0.0 82-83 0.001134434628720172 0.0 4.46898490101886E-4 0.0 0.0 84-85 0.001237565049512915 0.0 4.46898490101886E-4 0.0 0.0 86-87 0.0012719418564438292 0.0 4.46898490101886E-4 0.0 0.0 88 0.0012719418564438292 0.0 4.46898490101886E-4 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11735 0.0 79.437965 8 CACCGGC 11435 0.0 77.45825 9 CTTCACC 12985 0.0 74.21282 6 ATGCAGC 45 2.6593625E-9 73.02219 2 GTGTGGC 5135 0.0 72.48776 7 CCTTCAC 14370 0.0 70.62052 5 CGTAGTG 2705 0.0 68.54279 4 CAGCGTA 1165 0.0 67.66935 1 AGCAGCC 55 5.0495146E-9 67.33905 1 CGGGGCA 1285 0.0 66.116135 94 TTCACCG 14710 0.0 65.286736 7 TGTGGCA 5850 0.0 64.27005 8 CGGGCCC 780 0.0 63.788624 7 TGTGTGG 5605 0.0 62.47341 6 GCCTTCA 4320 0.0 61.93314 4 ATGTGTG 5445 0.0 59.9989 5 GGCCCGA 860 0.0 58.946404 9 ACCTTCA 5510 0.0 58.52442 4 GCGTAGT 1800 0.0 57.10887 3 GCTGCCT 140 0.0 56.68849 1 >>END_MODULE