FastQCFastQC Report
Mon 6 Mar 2017
1HL_21A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_21A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2591845
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA130760.504505477758122No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG80210.30947066664866146No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA61450.23708979510734635No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT58980.22755990423810063No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA54260.2093489386903924No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG48020.18527342491545598No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG47730.18415453084578748No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG42880.16544199209443466No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42810.16517191421554916No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA35980.13882002974714924No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC35130.1355405126463967No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33070.12759250649633755No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC32070.12373425108368749No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT32000.12346417320480199No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC31840.12284685233877797No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30400.11729096454456188No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30300.11690513900329688No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA29030.1120051546292313No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC26980.10409573103329867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAGC700.086.01
GCAGCAG450.086.03
GCAGCAA1100.086.03
AGCAGCA1900.086.02
GCAGCTA302.4598194E-885.999993
GCAGCAC159.829644E-485.999993
TGCAGCA302.4598194E-885.999992
CAGCTGC4850.084.22681
AGCTGCC1850.083.6756742
CAGCAGC4400.083.0681841
GCTGCCC1450.083.0344853
CAGCCCT153800.080.576071
CAGCGTA56850.080.327181
AGCTGCA1350.079.629632
AGCCCTT130450.078.0229952
GCTGCAT501.8189894E-1277.43
GCTGCAG501.8189894E-1277.43
GCTGCTT456.184564E-1176.444443
CAGCTAG42700.076.231861
CTGCTGC1750.076.1714251
Produced by FastQC (version 0.11.5)