##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_21A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2591845 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.77766803184604 12.0 12.0 12.0 2.0 27.0 2 14.547270380751936 12.0 12.0 12.0 12.0 22.0 3 13.716634289473328 12.0 12.0 12.0 12.0 22.0 4 13.706031803599366 12.0 12.0 12.0 12.0 22.0 5 22.438834112379407 22.0 22.0 22.0 22.0 27.0 6 26.788758972855245 27.0 22.0 32.0 22.0 32.0 7 34.942334514602535 37.0 37.0 37.0 27.0 41.0 8 37.576508240269 41.0 37.0 41.0 27.0 41.0 9 38.526444289685536 41.0 37.0 41.0 32.0 41.0 10-11 38.89291238480696 41.0 41.0 41.0 37.0 41.0 12-13 39.062966728334445 41.0 41.0 41.0 37.0 41.0 14-15 39.02169940717906 41.0 41.0 41.0 37.0 41.0 16-17 38.93042716674801 41.0 41.0 41.0 37.0 41.0 18-19 38.85781460696917 41.0 41.0 41.0 37.0 41.0 20-21 38.87318165245222 41.0 41.0 41.0 37.0 41.0 22-23 38.859949379688985 41.0 41.0 41.0 37.0 41.0 24-25 38.87167538953911 41.0 41.0 41.0 37.0 41.0 26-27 38.84469923934495 41.0 41.0 41.0 37.0 41.0 28-29 38.792583854358575 41.0 41.0 41.0 34.5 41.0 30-31 38.66316349936049 41.0 41.0 41.0 32.0 41.0 32-33 38.70697533996053 41.0 41.0 41.0 32.0 41.0 34-35 38.682461528370716 41.0 41.0 41.0 32.0 41.0 36-37 38.6097804845583 41.0 41.0 41.0 32.0 41.0 38-39 38.51300560025773 41.0 41.0 41.0 32.0 41.0 40-41 38.56344187248851 41.0 41.0 41.0 32.0 41.0 42-43 38.30860101587865 41.0 41.0 41.0 32.0 41.0 44-45 38.41107280720877 41.0 41.0 41.0 32.0 41.0 46-47 38.4229064623849 41.0 41.0 41.0 32.0 41.0 48-49 38.38560234118938 41.0 41.0 41.0 32.0 41.0 50-51 38.264237444754606 41.0 41.0 41.0 32.0 41.0 52-53 38.23161859601944 41.0 41.0 41.0 32.0 41.0 54-55 38.091490231861854 41.0 37.0 41.0 32.0 41.0 56-57 37.89524431437837 41.0 37.0 41.0 29.5 41.0 58-59 37.892699023282645 41.0 37.0 41.0 29.5 41.0 60-61 37.8928983021747 41.0 37.0 41.0 29.5 41.0 62-63 37.893507906529905 41.0 37.0 41.0 29.5 41.0 64-65 37.881516255794615 41.0 37.0 41.0 29.5 41.0 66-67 37.73925041813843 41.0 37.0 41.0 27.0 41.0 68-69 37.766869739509886 41.0 37.0 41.0 27.0 41.0 70-71 37.73996207334929 41.0 37.0 41.0 27.0 41.0 72-73 37.601675640325716 41.0 37.0 41.0 27.0 41.0 74-75 37.533164984788826 41.0 37.0 41.0 27.0 41.0 76-77 36.45278305608553 39.0 34.5 41.0 24.5 41.0 78-79 36.98618648105886 41.0 37.0 41.0 27.0 41.0 80-81 37.64765485590381 41.0 37.0 41.0 27.0 41.0 82-83 37.83483406608034 41.0 37.0 41.0 32.0 41.0 84-85 37.76144908356788 41.0 37.0 41.0 27.0 41.0 86-87 37.62555457598738 41.0 37.0 41.0 27.0 41.0 88-89 37.55051845307108 41.0 37.0 41.0 27.0 41.0 90-91 37.38521593690981 41.0 37.0 41.0 27.0 41.0 92-93 37.247481041497466 41.0 37.0 41.0 27.0 41.0 94-95 37.073618985703234 41.0 37.0 41.0 27.0 41.0 96-97 37.01249090898568 41.0 37.0 41.0 27.0 41.0 98-99 36.89549953797392 41.0 37.0 41.0 27.0 41.0 100 36.76243641112798 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4836550263373134 1101 2 -0.5195675887939597 1101 3 -0.0037677276395999826 1101 4 0.18857206017270478 1101 5 -1.0754621016411967 1101 6 -0.8613800377734648 1101 7 -0.2478821321489164 1101 8 -0.14858910293384753 1101 9 -0.08825891465505009 1101 10-11 -0.006395653162442727 1101 12-13 -0.030451911977181112 1101 14-15 -0.08535708685266741 1101 16-17 -0.2486930698419343 1101 18-19 -0.029647283241288847 1101 20-21 -0.1449315934789297 1101 22-23 0.01292305592846077 1101 24-25 -0.12850242496793385 1101 26-27 -0.010821581095136423 1101 28-29 0.04164705250583722 1101 30-31 -0.04872007263912792 1101 32-33 -0.06627073425148211 1101 34-35 -0.10248800235295619 1101 36-37 -0.0026470974666210623 1101 38-39 -0.024547852335679465 1101 40-41 -0.08535926584509212 1101 42-43 -0.033028333630575446 1101 44-45 -0.2070961211745228 1101 46-47 -0.02405006648076835 1101 48-49 -0.010570889381114057 1101 50-51 0.05348657376267596 1101 52-53 0.04086579216331643 1101 54-55 -0.06270845026108418 1101 56-57 0.017440748325483924 1101 58-59 0.03940020756265028 1101 60-61 0.003325981427117597 1101 62-63 -0.10625548993021283 1101 64-65 0.021298340727305742 1101 66-67 -0.01618503267645366 1101 68-69 -0.04413094411243179 1101 70-71 -0.2937562562536442 1101 72-73 -0.11173925006541907 1101 74-75 -0.2232089396808874 1101 76-77 -0.62585446384319 1101 78-79 -0.284446956133813 1101 80-81 -0.21238558768371973 1101 82-83 -0.13482006222259457 1101 84-85 -0.12235735562677519 1101 86-87 -0.11918854288128244 1101 88-89 -0.44993148857092535 1101 90-91 -0.31503334949761097 1101 92-93 -0.06534874721924666 1101 94-95 -0.05158014058667959 1101 96-97 -0.3323263097908651 1101 98-99 -0.3244619136933409 1101 100 -0.5803289511445726 1102 1 1.9707833851737355 1102 2 0.8719662168980395 1102 3 -0.07176131377964445 1102 4 0.006298870770503839 1102 5 -0.78184224016087 1102 6 -0.22723649149219938 1102 7 -0.1980587576278552 1102 8 -0.11071444157832389 1102 9 -0.17380335491313303 1102 10-11 -0.19167041888184144 1102 12-13 -0.19809583454625113 1102 14-15 -0.1355467626634166 1102 16-17 -0.1560972812790311 1102 18-19 -0.017362422227328977 1102 20-21 0.09788466424825515 1102 22-23 0.0284503329641268 1102 24-25 -0.004636548250317674 1102 26-27 -0.13139142490030764 1102 28-29 -0.12225125031111617 1102 30-31 0.019023075112542642 1102 32-33 -0.07612005042766867 1102 34-35 -0.03975304063037299 1102 36-37 -0.19449221077665868 1102 38-39 -0.1101889581273312 1102 40-41 -0.05623899200917748 1102 42-43 -0.18087501918564186 1102 44-45 0.024384575678290332 1102 46-47 -0.22781160647422638 1102 48-49 -0.2242354593978888 1102 50-51 -0.09215154189352148 1102 52-53 -0.058931530172316116 1102 54-55 -0.18366350185448965 1102 56-57 -0.18567418941783842 1102 58-59 -0.08030207035046999 1102 60-61 -0.045112176967457174 1102 62-63 -0.08548122324541652 1102 64-65 -0.025040323721519542 1102 66-67 -0.04570234298174114 1102 68-69 -0.30285435406135974 1102 70-71 -0.3500813332321755 1102 72-73 -0.17404799144138394 1102 74-75 -0.4024796801582724 1102 76-77 -0.24669025906118236 1102 78-79 -0.43469563961129865 1102 80-81 -0.3131810761859555 1102 82-83 -0.2376697660304785 1102 84-85 -0.1956137353095997 1102 86-87 -0.1696360257670264 1102 88-89 -0.31473732270916344 1102 90-91 -0.2978984383474881 1102 92-93 -0.22642813958182728 1102 94-95 -0.07304240990558242 1102 96-97 -0.1737845573581822 1102 98-99 -0.29583359664512443 1102 100 -0.6047423794512241 1103 1 1.2724930891393509 1103 2 0.7801946348087458 1103 3 0.3421601938999945 1103 4 0.9980218151190723 1103 5 2.8921244729312434 1103 6 -0.10629993713741115 1103 7 -0.4011593203294055 1103 8 -0.08862128292719262 1103 9 0.09597725294278092 1103 10-11 -0.04096872571117416 1103 12-13 -0.19739748927013068 1103 14-15 -0.2566110475403818 1103 16-17 -0.23216713976225378 1103 18-19 -0.15723863456277343 1103 20-21 -0.13097161446779637 1103 22-23 -0.002333311952483541 1103 24-25 -0.24557381734622652 1103 26-27 -0.26632620379101724 1103 28-29 -0.19372065006405847 1103 30-31 -0.08618662188238346 1103 32-33 -0.1541266806333752 1103 34-35 -0.2171562054804994 1103 36-37 -0.10412448628657955 1103 38-39 -0.13450547220134723 1103 40-41 -0.3449233244321377 1103 42-43 -0.13381883955156582 1103 44-45 -0.3771258647455795 1103 46-47 -0.4431046905880578 1103 48-49 -0.2411987796295989 1103 50-51 -0.10941558356213932 1103 52-53 -0.22012038028933745 1103 54-55 -0.19221810876356926 1103 56-57 -0.3090216205616656 1103 58-59 -0.4742979111069303 1103 60-61 -0.1625091919022097 1103 62-63 -0.17587800863831404 1103 64-65 -0.19423594345803252 1103 66-67 -0.2919706187060811 1103 68-69 -0.1181037879137321 1103 70-71 -0.5498622768593293 1103 72-73 -0.1774296594704481 1103 74-75 -0.2242741987614707 1103 76-77 -0.4757158960791159 1103 78-79 -0.28133035761155867 1103 80-81 -0.0011429702734915281 1103 82-83 -0.21775401515859727 1103 84-85 -0.3556741191530719 1103 86-87 -0.1517810463482263 1103 88-89 0.04505432632624462 1103 90-91 -0.38156899924058507 1103 92-93 -0.023026070013571598 1103 94-95 -0.14578459865889926 1103 96-97 0.02865958238313482 1103 98-99 -0.15420744670225872 1103 100 -0.05817022093499702 1104 1 1.6851314008736917 1104 2 -0.558474254100652 1104 3 0.03290506214708877 1104 4 0.10660414770029014 1104 5 -1.1263690609197 1104 6 -0.5678383231537758 1104 7 -0.24908361589213968 1104 8 -0.040064637827775584 1104 9 -0.11972572735098197 1104 10-11 -0.10008733994595076 1104 12-13 -0.01863661082187207 1104 14-15 0.027340284872565235 1104 16-17 0.07940206455612042 1104 18-19 0.018180534597789233 1104 20-21 -0.04995659174825562 1104 22-23 0.03237532206137672 1104 24-25 -0.09425713263758695 1104 26-27 -0.03653752171670277 1104 28-29 0.008145609697606915 1104 30-31 -0.028949013853882377 1104 32-33 -0.041667368351788525 1104 34-35 -0.036482216382658805 1104 36-37 0.09421254282124636 1104 38-39 0.023914836028794184 1104 40-41 0.01855385578846125 1104 42-43 0.027367457918586524 1104 44-45 -0.11189976797630408 1104 46-47 -0.161086791820928 1104 48-49 -0.2802755815086613 1104 50-51 -0.03175032078579676 1104 52-53 0.11573048465542257 1104 54-55 0.0169470520050794 1104 56-57 -0.06029259325561043 1104 58-59 -0.09491175199023871 1104 60-61 -0.08174540854181345 1104 62-63 0.009154624988333637 1104 64-65 -0.06101939044633298 1104 66-67 -0.5949827616472376 1104 68-69 -0.3270584534944163 1104 70-71 -0.29425926607125064 1104 72-73 -0.14177585886397992 1104 74-75 -0.3178743596174911 1104 76-77 -0.38951625121120514 1104 78-79 -0.2929599222105139 1104 80-81 -0.2024091616634891 1104 82-83 -0.3067931851320935 1104 84-85 -0.15745411908631013 1104 86-87 -0.11403201355594916 1104 88-89 -0.12568368119149653 1104 90-91 -0.23945971178839187 1104 92-93 -0.3646749777850573 1104 94-95 -0.13060891666528818 1104 96-97 -0.25926209769834685 1104 98-99 -0.3841458513333009 1104 100 -0.16165472136138703 1105 1 1.6128709382041713 1105 2 0.20416442998118534 1105 3 0.017121587311084596 1105 4 -0.26690092878522265 1105 5 -1.2258486011676553 1105 6 0.5338300806461973 1105 7 0.05920640248191944 1105 8 -0.15278449730515575 1105 9 -0.20250630209395126 1105 10-11 -0.22068259216788277 1105 12-13 -0.1439951543810878 1105 14-15 -0.08366347837039712 1105 16-17 -0.15487407615092508 1105 18-19 -0.08715002083388868 1105 20-21 -0.05468341462339055 1105 22-23 -0.05718744186258107 1105 24-25 -0.03544228963336593 1105 26-27 -0.10075612161497105 1105 28-29 -0.11240023059244209 1105 30-31 -0.17802065529746613 1105 32-33 -0.10576758746488935 1105 34-35 -0.06886262061193804 1105 36-37 -0.13179423237657062 1105 38-39 -0.12831368933776588 1105 40-41 -0.1886797865001384 1105 42-43 0.0028869162050924047 1105 44-45 -0.08441681367563802 1105 46-47 -0.2800153597403252 1105 48-49 -0.15775694858093203 1105 50-51 -0.1591970144756587 1105 52-53 -0.21608414822998867 1105 54-55 -0.12090175088458466 1105 56-57 -0.032009883690435004 1105 58-59 -0.12240519333572308 1105 60-61 -0.036560511549161845 1105 62-63 -0.32726060832290926 1105 64-65 -0.29664240782799567 1105 66-67 -0.2721012958644806 1105 68-69 -0.25148691899978104 1105 70-71 -0.39717175461108667 1105 72-73 -0.29205143194791106 1105 74-75 -0.41761673733099514 1105 76-77 -0.3669182757588274 1105 78-79 -0.2519187306101216 1105 80-81 -0.48497814374368176 1105 82-83 -0.3279204234075763 1105 84-85 -0.24303086120175976 1105 86-87 -0.21006207188280968 1105 88-89 -0.15076935373418365 1105 90-91 -0.2797791868541708 1105 92-93 -0.2928326742178058 1105 94-95 -0.16623633671621718 1105 96-97 -0.06391566242438529 1105 98-99 -0.37450485594457916 1105 100 -0.3689083929608046 1106 1 1.4157249782663541 1106 2 -0.6286804610862706 1106 3 -0.21395747339212434 1106 4 -0.32331972959747546 1106 5 -1.0938985264136747 1106 6 -0.8731008407245149 1106 7 -0.16660194467408473 1106 8 -0.01093230935654077 1106 9 -0.20897121979018607 1106 10-11 -0.06969684377267527 1106 12-13 0.02619548686674733 1106 14-15 -0.050435891547934375 1106 16-17 -0.06416718613969152 1106 18-19 0.09379403641055006 1106 20-21 -0.10858754357214906 1106 22-23 -0.031825015221897957 1106 24-25 -0.19339726616583164 1106 26-27 -0.105479197164847 1106 28-29 -0.08504845546432449 1106 30-31 -0.14421716529070494 1106 32-33 -0.1936102098791821 1106 34-35 -0.22468413339450422 1106 36-37 -0.3859183463001514 1106 38-39 -0.2105689165377882 1106 40-41 -0.4167229429777066 1106 42-43 -0.09702568486154206 1106 44-45 -0.12446244707616216 1106 46-47 -0.5751790374874375 1106 48-49 -0.30098799416970223 1106 50-51 -0.26887300617462273 1106 52-53 -0.19248532049142852 1106 54-55 -0.29553467536446476 1106 56-57 -0.31346197047943747 1106 58-59 -0.4260227653561657 1106 60-61 -0.17557632517743826 1106 62-63 -0.40078858713889076 1106 64-65 -0.5579654045328724 1106 66-67 -0.6722993381283757 1106 68-69 -0.44625711559159953 1106 70-71 -0.8256273596720405 1106 72-73 -0.39042915898058794 1106 74-75 -0.7103235752747707 1106 76-77 -0.29834953556796506 1106 78-79 -0.18152205102577312 1106 80-81 -0.5053923031147036 1106 82-83 -0.45470968412417534 1106 84-85 -0.5384530166454056 1106 86-87 -0.5040916090031828 1106 88-89 -0.5133579613762009 1106 90-91 -0.666959622881869 1106 92-93 -0.31425880166403886 1106 94-95 -0.442452669531356 1106 96-97 -0.3195538234020674 1106 98-99 -0.5500742506976692 1106 100 -0.6784110744344645 1107 1 1.289119278007048 1107 2 -0.48523478642088236 1107 3 -0.17056568065795297 1107 4 -0.24727698326537428 1107 5 -1.1326055845724845 1107 6 -0.1891213607599873 1107 7 -0.23936163500265195 1107 8 -0.16410292424666295 1107 9 -0.16175006813874404 1107 10-11 0.030126730320596096 1107 12-13 -0.1936824555918406 1107 14-15 -0.21417952580935662 1107 16-17 -0.22640175214692704 1107 18-19 -0.12866930329631998 1107 20-21 -0.07516465647350401 1107 22-23 -0.022934496570890417 1107 24-25 -0.022671992920827222 1107 26-27 -0.15001373585693756 1107 28-29 0.01084192474004908 1107 30-31 -0.13515527216401324 1107 32-33 -0.06745764169421165 1107 34-35 -0.2824059005721722 1107 36-37 0.040721591436280846 1107 38-39 -0.09379348819515343 1107 40-41 -0.12511935683583175 1107 42-43 -0.10682321602139666 1107 44-45 -0.06299158260792836 1107 46-47 -0.19520440689672114 1107 48-49 -0.1898724136854426 1107 50-51 -0.12514314174006103 1107 52-53 -0.20700500423442492 1107 54-55 -0.17011063579118257 1107 56-57 0.052676790460651546 1107 58-59 -0.43789213160737006 1107 60-61 -0.2372877874357684 1107 62-63 -0.14707746912623776 1107 64-65 -0.2420661188426365 1107 66-67 -0.24021059009749735 1107 68-69 -0.3887143935812034 1107 70-71 0.010477088954900182 1107 72-73 0.04939426544065384 1107 74-75 -0.17040866382448883 1107 76-77 -0.1933768694226572 1107 78-79 -0.4700240585018918 1107 80-81 -0.34689242846503276 1107 82-83 -0.32118614258457256 1107 84-85 -0.12676568900210583 1107 86-87 -0.17833417498048476 1107 88-89 0.05067790752606527 1107 90-91 -0.02679064315869084 1107 92-93 -0.18197192528191408 1107 94-95 -0.07369895111682467 1107 96-97 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0.27192434364532403 2223 14-15 0.272224797299657 2223 16-17 0.3773887492108514 2223 18-19 0.2086133064867397 2223 20-21 0.29550110957632825 2223 22-23 0.4595561820078231 2223 24-25 0.31478605929395087 2223 26-27 0.2984110060092604 2223 28-29 0.2812526230602117 2223 30-31 0.21234746136616423 2223 32-33 0.3120201697475906 2223 34-35 0.24042560688774017 2223 36-37 0.23403636405956263 2223 38-39 0.22392599362164134 2223 40-41 0.36548076373220084 2223 42-43 0.4000812866057899 2223 44-45 0.47021779857863777 2223 46-47 0.3204480407197323 2223 48-49 0.2724622082018442 2223 50-51 0.19621913261632784 2223 52-53 0.2692312535470123 2223 54-55 0.37027465579710395 2223 56-57 0.40405333734601356 2223 58-59 0.3892278780558627 2223 60-61 0.21155972067034412 2223 62-63 0.38599889446162194 2223 64-65 0.39190381547482644 2223 66-67 0.38136304885271954 2223 68-69 0.3064631812078531 2223 70-71 0.43498506278563553 2223 72-73 0.5119240600859314 2223 74-75 0.385133871980031 2223 76-77 0.4020793753830816 2223 78-79 0.6103092154243299 2223 80-81 0.3916508287469114 2223 82-83 0.4224687512218068 2223 84-85 0.4630114639099645 2223 86-87 0.42185855232862224 2223 88-89 0.5153741475742919 2223 90-91 0.5364733975157989 2223 92-93 0.418473405781846 2223 94-95 0.5336867306317075 2223 96-97 0.4942178387441416 2223 98-99 0.5414834174421657 2223 100 0.7404422203275871 2224 1 1.4485167909699257 2224 2 -0.751719437455618 2224 3 0.007014276208931136 2224 4 -0.3596984029873038 2224 5 -0.9147030470604385 2224 6 0.6740686543824914 2224 7 0.5726651625929051 2224 8 0.3015052985771831 2224 9 0.4021580675011407 2224 10-11 0.3227895119477253 2224 12-13 0.3279357245577472 2224 14-15 0.3263954294515088 2224 16-17 0.35767914538897827 2224 18-19 0.47444867036495 2224 20-21 0.4555103309077637 2224 22-23 0.5016089348061499 2224 24-25 0.4794387765923176 2224 26-27 0.4408353900091626 2224 28-29 0.35966379414766436 2224 30-31 0.44870766547651186 2224 32-33 0.37142181501206295 2224 34-35 0.3595777639072466 2224 36-37 0.3938223577373918 2224 38-39 0.296435025547801 2224 40-41 0.3524078307932186 2224 42-43 0.55559132075647 2224 44-45 0.455718553674366 2224 46-47 0.4765422548754117 2224 48-49 0.4118919143097912 2224 50-51 0.6066973575028172 2224 52-53 0.4816564925764837 2224 54-55 0.378597665039365 2224 56-57 0.4700814616853535 2224 58-59 0.3856865404432881 2224 60-61 0.4157946243478321 2224 62-63 0.5702859044340798 2224 64-65 0.44567191630626013 2224 66-67 0.5123533065665669 2224 68-69 0.6637565833379924 2224 70-71 0.6501836461062851 2224 72-73 0.5521072925594481 2224 74-75 0.3860888353724974 2224 76-77 0.7730286277553375 2224 78-79 0.5316737343673807 2224 80-81 0.4754301062938353 2224 82-83 0.47330061921967825 2224 84-85 0.3296652750689333 2224 86-87 0.5741780996030954 2224 88-89 0.6235223988765028 2224 90-91 0.5499541831616881 2224 92-93 0.5122819635814864 2224 94-95 0.6247994199197464 2224 96-97 0.6058525903201755 2224 98-99 0.5275502237516392 2224 100 0.8492815303295558 2225 1 1.3451946695220638 2225 2 1.6684668321812541 2225 3 0.9092551008137555 2225 4 1.072256707747652 2225 5 3.163273712170991 2225 6 -0.1198878434048467 2225 7 0.2648850795298685 2225 8 0.22322161943893093 2225 9 0.21497058182831807 2225 10-11 0.22886383350373052 2225 12-13 0.26031464674672833 2225 14-15 0.29572527065366216 2225 16-17 0.2881212155755719 2225 18-19 0.29617405145868503 2225 20-21 0.21939959376874185 2225 22-23 0.3368206895292971 2225 24-25 0.3020790955707042 2225 26-27 0.2533992937538443 2225 28-29 0.16777435735933466 2225 30-31 0.2235215122763563 2225 32-33 0.2877301961996821 2225 34-35 0.30429906898818615 2225 36-37 0.19479989855027924 2225 38-39 0.24855442848942033 2225 40-41 0.2877548886193253 2225 42-43 0.5130784563186452 2225 44-45 0.35180151337925736 2225 46-47 0.23126967652259367 2225 48-49 0.1508837324472836 2225 50-51 0.33377678990293447 2225 52-53 0.2122977969005717 2225 54-55 0.31724133739517413 2225 56-57 0.25756880828907924 2225 58-59 0.41714155548181964 2225 60-61 0.4437287932116405 2225 62-63 0.3857697317380584 2225 64-65 0.32802998904573144 2225 66-67 0.6107156787317862 2225 68-69 0.5136834829120502 2225 70-71 0.5870557520171218 2225 72-73 0.6172342595212612 2225 74-75 0.5316551071488931 2225 76-77 0.6305221855301468 2225 78-79 0.597993722840144 2225 80-81 0.48885334939113534 2225 82-83 0.39350781107900445 2225 84-85 0.30995708731181537 2225 86-87 0.43767551652731385 2225 88-89 0.4546589594960011 2225 90-91 0.5301561208141976 2225 92-93 0.348283486719275 2225 94-95 0.6896047304528281 2225 96-97 0.6289900213041335 2225 98-99 0.5519180633484169 2225 100 0.751371169135588 2226 1 1.1550082917779108 2226 2 1.271711419126179 2226 3 0.8506251981893573 2226 4 0.8979540842354172 2226 5 3.15783741797701 2226 6 -0.4193537862897294 2226 7 0.05529200759114161 2226 8 0.23409254788973755 2226 9 0.22299416473996558 2226 10-11 0.2062996621775497 2226 12-13 0.15855349030908883 2226 14-15 0.13623020878226555 2226 16-17 0.14913845826502836 2226 18-19 0.17778493562974518 2226 20-21 0.13672743799729403 2226 22-23 0.16671162059174094 2226 24-25 0.16422205230978904 2226 26-27 0.21098937485468383 2226 28-29 0.23307923617285553 2226 30-31 0.07141593732207951 2226 32-33 0.05039394900035887 2226 34-35 0.18958625287159236 2226 36-37 0.22955870599240313 2226 38-39 0.18496915235333944 2226 40-41 0.2445965224991582 2226 42-43 0.3527394086208986 2226 44-45 0.07723822440190276 2226 46-47 0.28394759780911016 2226 48-49 0.11384269584972628 2226 50-51 0.06892219670925925 2226 52-53 0.16241225878952292 2226 54-55 0.22115513722741298 2226 56-57 0.2835992972770569 2226 58-59 0.17242614986326288 2226 60-61 0.12555203867650278 2226 62-63 0.24934862633794097 2226 64-65 0.18319014347153484 2226 66-67 0.14631164781416572 2226 68-69 0.24152996159514117 2226 70-71 0.34839183174722166 2226 72-73 0.2277905007743115 2226 74-75 0.38581561053037206 2226 76-77 0.5550861569261087 2226 78-79 0.5485895665050506 2226 80-81 0.3850335942208005 2226 82-83 0.401020594439494 2226 84-85 0.3896100018294746 2226 86-87 0.39767886960037657 2226 88-89 0.26707794534080165 2226 90-91 0.39597408620339536 2226 92-93 0.17802935771781137 2226 94-95 0.42085205149869154 2226 96-97 0.4287656346877995 2226 98-99 0.5452802513079007 2226 100 0.7138591714786813 2227 1 1.1821488369008328 2227 2 1.46778997642574 2227 3 0.629230022604288 2227 4 -0.1593968547015674 2227 5 3.191779155074652 2227 6 0.08691178773344177 2227 7 0.3345904379864919 2227 8 0.18497625328701872 2227 9 0.21502925606475287 2227 10-11 0.22932673849989982 2227 12-13 0.09186712865779612 2227 14-15 0.23675812000070096 2227 16-17 0.1526533707936082 2227 18-19 0.039108695487939826 2227 20-21 0.0988623315159316 2227 22-23 0.1271311237643502 2227 24-25 0.19646702366642899 2227 26-27 0.1624600764242885 2227 28-29 0.07323645352842334 2227 30-31 0.22557885766014607 2227 32-33 0.07732191673483868 2227 34-35 0.14166514412958975 2227 36-37 0.05803829599420851 2227 38-39 -0.01113445182884476 2227 40-41 0.16290772038238543 2227 42-43 0.267308987576385 2227 44-45 0.26641989779054853 2227 46-47 0.30060955480553986 2227 48-49 0.17815217256254812 2227 50-51 0.2101930370000673 2227 52-53 0.3536959436355147 2227 54-55 0.28656860345907376 2227 56-57 0.42729454736054606 2227 58-59 0.5554321611150925 2227 60-61 0.45776875201172373 2227 62-63 0.3845857368268071 2227 64-65 0.25318012471848306 2227 66-67 0.3041911401973394 2227 68-69 0.319289614278361 2227 70-71 0.20164278866658236 2227 72-73 0.377542600859087 2227 74-75 0.3462569494467971 2227 76-77 0.5175517977409712 2227 78-79 0.44344660660574675 2227 80-81 0.2502049405870821 2227 82-83 0.27746817580975147 2227 84-85 0.308542233956274 2227 86-87 0.25143654422210915 2227 88-89 0.3145385767791282 2227 90-91 0.37604896996018056 2227 92-93 0.33629804012333864 2227 94-95 0.5794874459549817 2227 96-97 0.4097698220127981 2227 98-99 0.4743352773823446 2227 100 0.444151770445977 2228 1 1.468491036315383 2228 2 1.643542569393107 2228 3 -0.12304672922976145 2228 4 -0.020862829910269554 2228 5 3.225943984679656 2228 6 -0.06188930366188217 2228 7 0.20653037510477645 2228 8 0.35381818165968326 2228 9 0.1611822682435644 2228 10-11 0.08111697015036867 2228 12-13 0.07132260551081515 2228 14-15 0.08047085174150936 2228 16-17 0.059938073189613306 2228 18-19 0.019440541102483166 2228 20-21 0.1771920810732297 2228 22-23 0.15165137962788577 2228 24-25 0.1968415132042196 2228 26-27 0.18059503721356407 2228 28-29 0.20620418147464648 2228 30-31 0.11461476167517759 2228 32-33 0.12786083834952677 2228 34-35 0.028993032532611096 2228 36-37 0.12749548944583466 2228 38-39 0.12877330358296035 2228 40-41 0.17839053632687296 2228 42-43 0.4001055073985853 2228 44-45 0.31419780606997705 2228 46-47 0.18412117346768042 2228 48-49 0.12471852361599645 2228 50-51 0.14588112199880499 2228 52-53 0.24123931033711443 2228 54-55 0.08584853551791127 2228 56-57 0.4200753467534142 2228 58-59 0.2972923215718879 2228 60-61 0.3152662222874625 2228 62-63 0.3955697092166801 2228 64-65 0.1853704141912189 2228 66-67 0.37759063786638336 2228 68-69 0.3397234619889602 2228 70-71 0.4406425537636096 2228 72-73 0.3643537027346966 2228 74-75 0.33008433190497044 2228 76-77 0.37583574121969576 2228 78-79 0.45072498469111366 2228 80-81 0.23327340576723543 2228 82-83 0.07004207989707112 2228 84-85 0.3062112737452196 2228 86-87 0.36791137346572356 2228 88-89 0.23402965046638258 2228 90-91 0.43884982645253245 2228 92-93 0.2675681423964775 2228 94-95 0.5784791701427636 2228 96-97 0.408847827867298 2228 98-99 0.4997057012918589 2228 100 0.5672752571287845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 27.0 13 25.0 14 127.0 15 444.0 16 1657.0 17 4322.0 18 7006.0 19 8904.0 20 10686.0 21 12341.0 22 14019.0 23 15837.0 24 18129.0 25 20445.0 26 23469.0 27 26480.0 28 29588.0 29 33543.0 30 38515.0 31 44656.0 32 52235.0 33 62115.0 34 76071.0 35 98395.0 36 139425.0 37 229363.0 38 564117.0 39 1058766.0 40 1138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.064883362230812 19.629387105125186 19.287185835086397 36.018543697557604 2 19.598830871926456 51.04586801971698 11.746997598078462 17.608303510278102 3 40.3864042795769 15.530288269553155 24.168652060597758 19.91465539027218 4 19.677990003260227 16.06720309277754 21.203621358530313 43.05118554543193 5 17.742534758058447 35.60548566754571 26.06691372362159 20.585065850774257 6 17.6680704285943 27.644284283975313 30.80118602771385 23.886459259716535 7 20.412717581491176 26.433139327390332 36.26652056739504 16.887622523723447 8 20.76177394867363 27.591464767376134 34.32184409175703 17.324917192193208 9 20.77651248434995 28.009776819215652 27.999320947047373 23.21438974938702 10-11 19.60638078280144 30.147269609100853 28.601575325684987 21.644774282412722 12-13 24.308880353570526 26.531428384027596 27.262066211521134 21.89762505088074 14-15 21.78774193672847 27.245880830065065 28.622487070021545 22.343890163184913 16-17 21.004631835622884 29.411808962341496 28.26793268887607 21.315626513159543 18-19 21.506803068856357 29.298511292149026 29.824584417663864 19.370101221330753 20-21 22.922069028047588 28.732620970775645 29.878850780042786 18.46645922113398 22-23 22.127268413041676 30.231553198590195 28.59947257648509 19.04170581188304 24-25 22.289064353771153 28.263746481753344 26.60789900630632 22.83929015816918 26-27 20.385574754663182 26.251184002129758 29.928487235926532 23.434754007280528 28-29 19.838377057181493 27.72034099392901 30.745456404439324 21.695825544450166 30-31 20.213787056053263 29.875665492037246 28.03782223126855 21.872725220640937 32-33 20.759516869257226 29.855218965640308 28.860830798138004 20.52443336696446 34-35 21.611419664370363 29.078050577870208 29.616643742199088 19.693886015560345 36-37 21.966089793178217 28.02254764463153 28.626750442252526 21.384612119937728 38-39 21.602056466003685 29.211424382433226 28.813384391307284 20.373134760255805 40-41 21.02725201866805 29.85290577079784 29.574142837741068 19.545699372793045 42-43 20.528982658286015 29.748946984828944 29.266819016429622 20.455251340455423 44-45 20.62206652018157 29.951694642233623 28.861293788787524 20.564945048797284 46-47 19.96159795415445 27.86237614250234 29.030584665649865 23.145441237693344 48-49 20.91811045799421 27.98709027738927 30.23255634499748 20.862242919619035 50-51 21.873240876672796 30.41557269049654 28.23511822659148 19.476068206239187 52-53 20.512619188079977 30.277293023747525 28.255865502331 20.9542222858415 54-55 22.638856569964585 27.962294716946033 28.309679739719783 21.089168973369592 56-57 21.828283878705047 28.52533647328115 29.093316915877192 20.553062732136613 58-59 21.28334953402589 27.808858582424612 29.02325452169111 21.884537361858385 60-61 19.70304471189943 30.33434188402207 29.478674978914576 20.48393842516392 62-63 20.46906168177129 31.03903072136191 27.375623447951998 21.116284148914804 64-65 19.967945570745744 30.147618980558804 28.672413031429585 21.21202241726587 66-67 20.348419755039362 30.14055624468284 29.198736807177898 20.312287193099895 68-69 20.907133721345218 29.970735132695047 29.20350175261252 19.91862939334721 70-71 19.92817857549352 30.72378942413609 29.082236784992933 20.265795215377462 72-73 20.062889563226197 30.70015760973361 28.429130600016588 20.807822227023607 74-75 20.176187233418666 29.028838530081853 30.34922998867602 20.445744247823463 76-77 19.54175500463955 30.311380503077924 28.63782363528683 21.509040856995693 78-79 19.74988859287496 29.324477351076162 28.496765817400345 22.42886823864853 80-81 21.046781235187893 29.244990191333148 28.379755621536123 21.328472951942835 82-83 20.502094068125214 27.863220987366144 29.94324506287992 21.691439881628725 84-85 20.956249312748255 29.004589394813348 28.381635475886867 21.65752581655153 86-87 21.220655556177164 29.17352310805623 29.329724578437364 20.27609675732924 88-89 20.48448113216647 28.43204358285314 29.603757169120836 21.47971811585955 90-91 20.87480154098721 28.746124864719917 28.940561646240422 21.43851194805245 92-93 21.268555797125213 28.31027704202991 29.75202992462898 20.6691372362159 94-95 21.593170116268524 28.331053747427028 28.737771741751533 21.338004394552915 96-97 21.511780218338675 28.71549031674348 28.990255975955353 20.7824734889625 98-99 22.18367610717462 29.445838775081075 28.27734683208294 20.09313828566137 100 21.68250802034844 30.66718881723251 27.418499177227034 20.231803985192016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 9.0 2 16.0 3 22.5 4 23.0 5 25.5 6 36.5 7 47.0 8 50.5 9 64.0 10 73.0 11 78.0 12 96.5 13 112.5 14 126.5 15 152.0 16 209.5 17 286.5 18 420.5 19 673.0 20 1126.0 21 1778.0 22 2752.0 23 4176.0 24 6255.0 25 9301.0 26 13490.5 27 18339.5 28 23576.0 29 29687.5 30 36860.0 31 45119.5 32 54181.5 33 64275.5 34 75480.5 35 87605.0 36 99671.0 37 109636.5 38 116879.0 39 122041.0 40 125307.5 41 126227.5 42 124469.0 43 121834.5 44 120326.0 45 116599.5 46 108610.0 47 99672.0 48 93250.5 49 90094.0 50 87392.5 51 81305.5 52 71290.5 53 59887.5 54 49672.0 55 40566.5 56 31413.0 57 23222.0 58 16911.5 59 12713.0 60 10281.0 61 8728.5 62 7313.5 63 5954.5 64 4852.5 65 4330.5 66 4520.0 67 4069.0 68 2912.5 69 2603.0 70 2878.0 71 2692.5 72 1847.0 73 1011.5 74 600.5 75 388.0 76 256.5 77 190.0 78 143.0 79 99.0 80 79.5 81 56.5 82 37.5 83 28.0 84 17.5 85 14.0 86 12.5 87 10.5 88 14.0 89 15.0 90 14.5 91 15.5 92 13.0 93 9.0 94 12.5 95 15.0 96 13.5 97 13.0 98 36.0 99 90.5 100 98.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.323593810586667 2 0.006559034201505106 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.858255412650062E-4 30-31 0.009549182146308904 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.716510825300124E-4 40-41 0.0014275545026805228 42-43 7.716510825300125E-5 44-45 0.0 46-47 0.0021606230310840346 48-49 0.0 50-51 0.0 52-53 0.0014661370568070234 54-55 0.0186739561972263 56-57 0.011362562190254432 58-59 0.015124361217588244 60-61 2.7007787888550433E-4 62-63 0.011941300502151942 64-65 1.3503893944275216E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.736214935692528E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2591845.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.16692800685429 #Duplication Level Percentage of deduplicated Percentage of total 1 86.68450532411349 65.15807971007416 2 8.262087430828997 12.420714621989177 3 2.2624408899730937 5.101821944891127 4 0.9877000434140387 2.969695122226796 5 0.5146513158391158 1.9342379203155826 6 0.31545104091006854 1.4226891409064624 7 0.2241805888043825 1.1795676325435256 8 0.14447759266251978 0.8687949445013795 9 0.11197558587821342 0.7575174722009699 >10 0.4636461783117499 5.858987969829818 >50 0.020076590478882616 1.0207244025254203 >100 0.008389499062094767 1.0991316131997184 >500 4.179197233643959E-4 0.2080375047958584 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.858255412650062E-5 0.0 0.0 0.0 0.0 8 3.858255412650062E-5 0.0 0.0 0.0 0.0 9 3.858255412650062E-5 0.0 0.0 0.0 0.0 10-11 3.858255412650062E-5 0.0 0.0 0.0 0.0 12-13 3.858255412650062E-5 0.0 0.0 0.0 0.0 14-15 3.858255412650062E-5 0.0 0.0 0.0 0.0 16-17 3.858255412650062E-5 0.0 0.0 0.0 0.0 18-19 3.858255412650062E-5 0.0 0.0 0.0 0.0 20-21 3.858255412650062E-5 0.0 0.0 0.0 0.0 22-23 3.858255412650062E-5 0.0 0.0 0.0 0.0 24-25 3.858255412650062E-5 0.0 0.0 0.0 0.0 26-27 3.858255412650062E-5 0.0 0.0 0.0 0.0 28-29 3.858255412650062E-5 0.0 0.0 0.0 0.0 30-31 3.858255412650062E-5 0.0 0.0 0.0 0.0 32-33 3.858255412650062E-5 0.0 0.0 0.0 0.0 34-35 3.858255412650062E-5 0.0 0.0 0.0 0.0 36-37 3.858255412650062E-5 0.0 0.0 0.0 0.0 38-39 3.858255412650062E-5 0.0 0.0 0.0 0.0 40-41 3.858255412650062E-5 0.0 0.0 0.0 0.0 42-43 3.858255412650062E-5 0.0 0.0 0.0 0.0 44-45 3.858255412650062E-5 0.0 0.0 0.0 0.0 46-47 3.858255412650062E-5 0.0 0.0 0.0 0.0 48-49 3.858255412650062E-5 0.0 0.0 0.0 0.0 50-51 3.858255412650062E-5 0.0 0.0 0.0 0.0 52-53 3.858255412650062E-5 0.0 0.0 0.0 3.858255412650062E-5 54-55 3.858255412650062E-5 0.0 0.0 0.0 3.858255412650062E-5 56-57 5.787383118975093E-5 0.0 0.0 0.0 3.858255412650062E-5 58-59 1.350389394427522E-4 0.0 0.0 0.0 3.858255412650062E-5 60-61 1.543302165060025E-4 0.0 0.0 0.0 3.858255412650062E-5 62-63 1.543302165060025E-4 0.0 0.0 0.0 3.858255412650062E-5 64-65 1.929127706325031E-4 0.0 0.0 0.0 3.858255412650062E-5 66-67 2.122040476957534E-4 0.0 0.0 0.0 3.858255412650062E-5 68-69 2.8936915594875466E-4 0.0 0.0 0.0 3.858255412650062E-5 70-71 3.08660433012005E-4 0.0 0.0 0.0 3.858255412650062E-5 72-73 3.2795171007525526E-4 0.0 0.0 0.0 3.858255412650062E-5 74-75 3.472429871385056E-4 0.0 0.0 0.0 3.858255412650062E-5 76-77 4.6299064951800745E-4 0.0 0.0 0.0 3.858255412650062E-5 78-79 5.59447034834259E-4 0.0 3.858255412650062E-5 0.0 3.858255412650062E-5 80-81 6.1732086602401E-4 0.0 3.858255412650062E-5 0.0 3.858255412650062E-5 82-83 9.066900219727647E-4 0.0 3.858255412650062E-5 0.0 3.858255412650062E-5 84-85 0.0010224376843522664 0.0 3.858255412650062E-5 0.0 3.858255412650062E-5 86-87 0.0010803115155420173 0.0 3.858255412650062E-5 0.0 3.858255412650062E-5 88 0.0010803115155420173 0.0 3.858255412650062E-5 0.0 3.858255412650062E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10205 0.0 78.882256 8 CACCGGC 9895 0.0 78.12788 9 CTTCACC 11230 0.0 75.067986 6 CCTTCAC 12370 0.0 71.03369 5 CGTAGTG 2375 0.0 70.95109 4 TTCACCG 12310 0.0 67.41436 7 GTGTGGC 4010 0.0 64.61345 7 GCGTAGT 1640 0.0 63.824764 3 GCTGCTA 75 0.0 62.590565 2 AGGCGCG 400 0.0 62.19337 5 GCCTTCA 3875 0.0 61.05019 4 CAGCGTA 1035 0.0 60.87954 1 CGGGGCA 1320 0.0 59.384182 94 TGTGGCA 4565 0.0 57.374863 8 CAGCAGC 65 1.8779247E-8 57.022915 1 AGCGTAG 1470 0.0 56.84245 2 ACCTTCA 4970 0.0 56.382736 4 AACGGGC 780 0.0 55.36323 5 GGGCCCG 755 0.0 55.331352 8 ACGGGCC 790 0.0 54.06827 6 >>END_MODULE