FastQCFastQC Report
Tue 7 Mar 2017
1HL_21A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_21A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2591845
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG102050.078.8822568
CACCGGC98950.078.127889
CTTCACC112300.075.0679866
CCTTCAC123700.071.033695
CGTAGTG23750.070.951094
TTCACCG123100.067.414367
GTGTGGC40100.064.613457
GCGTAGT16400.063.8247643
GCTGCTA750.062.5905652
AGGCGCG4000.062.193375
GCCTTCA38750.061.050194
CAGCGTA10350.060.879541
CGGGGCA13200.059.38418294
TGTGGCA45650.057.3748638
CAGCAGC651.8779247E-857.0229151
AGCGTAG14700.056.842452
ACCTTCA49700.056.3827364
AACGGGC7800.055.363235
GGGCCCG7550.055.3313528
ACGGGCC7900.054.068276
Produced by FastQC (version 0.11.5)