FastQCFastQC Report
Mon 6 Mar 2017
1HL_22A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_22A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2074229
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA120990.5833010723502564No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA57040.2749937446636798No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG55180.2660265573376903No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA48880.23565382607224178No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT46080.22215483439870912No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG37710.18180249143175609No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG36280.17490836354134476No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG33450.16126473981416709No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT33120.15967378722407216No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA32020.15437061192375576No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG30400.14656048102692615No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA29870.14400531474586462No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC29730.14333036516218797No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT29450.14198046599483471No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC29110.14034130272019146No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA27730.13368822825252177No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC25210.12153913574634238No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA24510.11816438782795921No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA24010.11575385360054265No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA23870.11507890401686602No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA23390.11276479115854615No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA22470.10832940818009967No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC22450.10823298681100302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCT159.829365E-486.000013
GCTGCAC302.4594556E-886.000013
GCAGCTA159.829365E-486.000013
GCTGCCG202.8583798E-586.03
GCTGCCC1250.086.03
GCTGCCA450.086.03
GCTGCAT258.3585655E-786.03
GCTGCAG450.086.03
TGCTGCC550.086.02
TGCTGCA750.086.02
AGCTGCT1050.086.02
AGCTGCC1500.086.02
GCAGCCT357.2759576E-1086.03
GCAGCCG258.3585655E-786.03
GCAGCAA450.086.03
AGCAGCG800.086.02
AGCAGCC1050.086.02
CAGCTGC4200.084.976191
CAGCAGC3300.084.696971
AGCAGCA1050.081.904762
Produced by FastQC (version 0.11.5)