##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_22A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2074229 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.718544577286307 12.0 12.0 12.0 2.0 27.0 2 14.07500232616553 12.0 12.0 12.0 12.0 22.0 3 13.714082678431359 12.0 12.0 12.0 12.0 22.0 4 13.738361579169899 12.0 12.0 12.0 12.0 22.0 5 22.4097016288944 22.0 22.0 22.0 22.0 27.0 6 26.71897895555409 27.0 22.0 32.0 22.0 32.0 7 34.83119993019093 37.0 37.0 37.0 27.0 41.0 8 37.47318690462818 41.0 37.0 41.0 27.0 41.0 9 38.4515489851892 41.0 37.0 41.0 32.0 41.0 10-11 38.846387983197616 41.0 41.0 41.0 34.5 41.0 12-13 39.02508739391841 41.0 41.0 41.0 37.0 41.0 14-15 38.968171306061194 41.0 41.0 41.0 37.0 41.0 16-17 38.85794986956599 41.0 41.0 41.0 37.0 41.0 18-19 38.78749814991498 41.0 41.0 41.0 34.5 41.0 20-21 38.811551424649835 41.0 41.0 41.0 37.0 41.0 22-23 38.78678053387548 41.0 41.0 41.0 34.5 41.0 24-25 38.786119083283474 41.0 41.0 41.0 34.5 41.0 26-27 38.752218294122784 41.0 41.0 41.0 32.0 41.0 28-29 38.69665090016579 41.0 41.0 41.0 32.0 41.0 30-31 38.58353489417031 41.0 41.0 41.0 32.0 41.0 32-33 38.65846345798849 41.0 41.0 41.0 32.0 41.0 34-35 38.61991202514284 41.0 41.0 41.0 32.0 41.0 36-37 38.551509982745394 41.0 41.0 41.0 32.0 41.0 38-39 38.441999894900704 41.0 41.0 41.0 32.0 41.0 40-41 38.482149270885714 41.0 41.0 41.0 32.0 41.0 42-43 38.22112842892467 41.0 41.0 41.0 32.0 41.0 44-45 38.348011236946355 41.0 41.0 41.0 32.0 41.0 46-47 38.35304853032139 41.0 41.0 41.0 32.0 41.0 48-49 38.30368488725208 41.0 41.0 41.0 32.0 41.0 50-51 38.17504094292385 41.0 41.0 41.0 32.0 41.0 52-53 38.148141550426686 41.0 37.0 41.0 32.0 41.0 54-55 38.01445284006732 41.0 37.0 41.0 32.0 41.0 56-57 37.810719308234525 41.0 37.0 41.0 27.0 41.0 58-59 37.78835364851229 41.0 37.0 41.0 27.0 41.0 60-61 37.81659594962755 41.0 37.0 41.0 27.0 41.0 62-63 37.811916620585286 41.0 37.0 41.0 27.0 41.0 64-65 37.781379731938955 41.0 37.0 41.0 27.0 41.0 66-67 37.64113677901524 41.0 37.0 41.0 27.0 41.0 68-69 37.657073785006375 41.0 37.0 41.0 27.0 41.0 70-71 37.63162577516754 41.0 37.0 41.0 27.0 41.0 72-73 37.52334168503092 41.0 37.0 41.0 27.0 41.0 74-75 37.410803966196596 41.0 37.0 41.0 27.0 41.0 76-77 36.303903040599664 39.0 34.5 41.0 24.5 41.0 78-79 36.85318496655866 41.0 37.0 41.0 27.0 41.0 80-81 37.541332948290666 41.0 37.0 41.0 27.0 41.0 82-83 37.72570723868965 41.0 37.0 41.0 27.0 41.0 84-85 37.660370431615796 41.0 37.0 41.0 27.0 41.0 86-87 37.53563878433866 41.0 37.0 41.0 27.0 41.0 88-89 37.442802120691596 41.0 37.0 41.0 27.0 41.0 90-91 37.25810650608009 41.0 37.0 41.0 27.0 41.0 92-93 37.1417013261313 41.0 37.0 41.0 27.0 41.0 94-95 36.968284601169884 41.0 37.0 41.0 27.0 41.0 96-97 36.89990618200787 41.0 37.0 41.0 27.0 41.0 98-99 36.76651348525163 41.0 37.0 41.0 27.0 41.0 100 36.63210715885276 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3436457343238946 1101 2 -0.4653249958723933 1101 3 -0.11883382382126051 1101 4 0.014677718071185453 1101 5 -1.140349095694173 1101 6 -0.9468715277454933 1101 7 -0.7331354835145021 1101 8 -0.669141989991175 1101 9 -0.4685742003709592 1101 10-11 -0.4727736921534671 1101 12-13 -0.38160641873704293 1101 14-15 -0.35907777189814993 1101 16-17 -0.37865238354761743 1101 18-19 -0.4148584750593258 1101 20-21 -0.3019019209823455 1101 22-23 -0.19775096315906637 1101 24-25 -0.3212437269877171 1101 26-27 -0.10168781420144768 1101 28-29 -0.24542040496236694 1101 30-31 -0.3576873316372442 1101 32-33 -0.3483334094688786 1101 34-35 -0.3572494275777274 1101 36-37 -0.363009057084021 1101 38-39 -0.4150174676494274 1101 40-41 -0.3754217879963875 1101 42-43 -0.3426662010371402 1101 44-45 -0.3487991810436242 1101 46-47 -0.405199169446 1101 48-49 -0.2530242100499791 1101 50-51 -0.5218015253181463 1101 52-53 -0.3941963000376205 1101 54-55 -0.3237572542688625 1101 56-57 0.04830574688177336 1101 58-59 -0.3966065122151292 1101 60-61 -0.3304696508803886 1101 62-63 -0.5349139245904837 1101 64-65 -0.3423977766092676 1101 66-67 -0.5039993713590363 1101 68-69 -0.4472720518012139 1101 70-71 -0.595070554027771 1101 72-73 -0.384846012324644 1101 74-75 -0.11465699738274537 1101 76-77 -0.747362375070658 1101 78-79 -0.4039710647619401 1101 80-81 -0.5390428906121656 1101 82-83 -0.5049003394594749 1101 84-85 -0.2976221480844785 1101 86-87 -0.5354457047746308 1101 88-89 -0.6904514026704689 1101 90-91 -0.20125343290411735 1101 92-93 -0.30830350916284033 1101 94-95 -0.1462455978205668 1101 96-97 -0.6294061228683674 1101 98-99 -0.7706676152493941 1101 100 -0.8600217614993042 1102 1 1.5354785186074444 1102 2 0.5479183253577986 1102 3 -0.09840676728917153 1102 4 -0.03128616487300384 1102 5 -0.8061400880716008 1102 6 -0.2396252729062418 1102 7 -0.16573942224535898 1102 8 -0.4357673777325388 1102 9 -0.22287121626535367 1102 10-11 -0.465429449962393 1102 12-13 -0.4208668847480581 1102 14-15 -0.23153671339429138 1102 16-17 -0.44178689673683635 1102 18-19 -0.3005042297422804 1102 20-21 -0.14112451458130693 1102 22-23 -0.11360026702806181 1102 24-25 -0.14415172708389434 1102 26-27 -0.2549785060041714 1102 28-29 -0.32350009332652263 1102 30-31 -0.4943875023636721 1102 32-33 -0.41289144443172887 1102 34-35 -0.261039073380509 1102 36-37 -0.3682691143134491 1102 38-39 -0.15340025553987147 1102 40-41 -0.3715311490948068 1102 42-43 -0.3134305023813013 1102 44-45 -0.21694066756585073 1102 46-47 -0.6779707086289122 1102 48-49 -0.6337399385228224 1102 50-51 -0.3882339441028577 1102 52-53 -0.3255660790551076 1102 54-55 -0.3787888747271779 1102 56-57 -0.31243859630239257 1102 58-59 -0.1141222022144035 1102 60-61 -0.30495146009961616 1102 62-63 -0.5208506355819011 1102 64-65 -0.6564304069985738 1102 66-67 -0.5366832629597766 1102 68-69 -0.7622034005400025 1102 70-71 -0.5765996376356242 1102 72-73 -0.4806006952784543 1102 74-75 -0.6214103097159196 1102 76-77 -0.4967570176272247 1102 78-79 -0.699139025903655 1102 80-81 -0.564503371050769 1102 82-83 -0.6034918464207877 1102 84-85 -0.7004854226654871 1102 86-87 -0.5548718475596388 1102 88-89 -0.576728364220962 1102 90-91 -0.7704300986890971 1102 92-93 -0.6098670987461219 1102 94-95 -0.48771588901733054 1102 96-97 -0.41177922023052815 1102 98-99 -0.28644538234007655 1102 100 -0.5712656598329175 1103 1 1.6789871122244602 1103 2 0.4780512445726188 1103 3 0.3562457752718675 1103 4 1.1367895408594446 1103 5 2.79757600063002 1103 6 0.040380228390141326 1103 7 -0.45771271012104364 1103 8 -0.4167465838189912 1103 9 -0.4769361315626526 1103 10-11 -0.3728978915166721 1103 12-13 -0.44175762604157853 1103 14-15 -0.48623902665241303 1103 16-17 -0.327084250440592 1103 18-19 -0.36890440094854426 1103 20-21 -0.29115294616911314 1103 22-23 -0.26321486791270843 1103 24-25 -0.3391631243885058 1103 26-27 -0.19808665211662912 1103 28-29 -0.36100912852741374 1103 30-31 -0.07676540688781586 1103 32-33 -0.33201745372225844 1103 34-35 -0.4723943165106661 1103 36-37 -0.20839323885490302 1103 38-39 0.03425901685817223 1103 40-41 -0.213017337880558 1103 42-43 -0.217199137097694 1103 44-45 -0.44052118508242444 1103 46-47 -0.46982619968745354 1103 48-49 -0.27919217962268306 1103 50-51 -0.2457608523496262 1103 52-53 -0.1818215834351733 1103 54-55 -0.14834160452964795 1103 56-57 -0.36183585897572357 1103 58-59 -0.41496478866246633 1103 60-61 -0.1911498669439169 1103 62-63 -0.15600706482116777 1103 64-65 -0.3711903481865093 1103 66-67 -0.23981369795690455 1103 68-69 -0.31945727920064826 1103 70-71 -0.3502271505316017 1103 72-73 -0.3217245509224611 1103 74-75 -0.5377212217716547 1103 76-77 -0.43464417444376835 1103 78-79 -0.4859832183355408 1103 80-81 -0.3170924340788517 1103 82-83 -0.4336374704232142 1103 84-85 -0.41381669198477766 1103 86-87 -0.2868193311482585 1103 88-89 -0.38765707186036025 1103 90-91 -0.7522027372637936 1103 92-93 -0.2821705384131832 1103 94-95 -0.2518999209495192 1103 96-97 -0.19900949018876446 1103 98-99 -0.0944612398885738 1103 100 -0.048643737043484236 1104 1 1.6466813511892973 1104 2 -0.44938703312591954 1104 3 -0.048223582998254244 1104 4 0.048618954140080106 1104 5 -1.0396164949615745 1104 6 -0.5278862576963945 1104 7 -0.11082233566943955 1104 8 -0.031600099257794056 1104 9 0.05092155210351734 1104 10-11 -0.08106416055031929 1104 12-13 -0.031121265592318537 1104 14-15 -0.20162511816890571 1104 16-17 -0.03460086768759396 1104 18-19 0.01786890394465246 1104 20-21 -0.08022200515349454 1104 22-23 0.051516436108329344 1104 24-25 -0.11046124705629268 1104 26-27 -0.03184329701650768 1104 28-29 0.08815258233401835 1104 30-31 -0.005842190962319194 1104 32-33 0.05096711270397947 1104 34-35 -0.2082898764905181 1104 36-37 -0.32325182849701406 1104 38-39 -0.19648422783959774 1104 40-41 -0.15007692009832851 1104 42-43 -0.05977905272445838 1104 44-45 -0.07162475972025817 1104 46-47 -0.34922145921296277 1104 48-49 -0.32816254689044655 1104 50-51 -0.18447201086096499 1104 52-53 -0.17696849238429024 1104 54-55 -0.3385398121365242 1104 56-57 -0.26281185891667747 1104 58-59 -0.2324318570830286 1104 60-61 -0.1969512189577003 1104 62-63 -0.30808631957139454 1104 64-65 -0.4669321075265813 1104 66-67 -0.5296540358434925 1104 68-69 -0.7275522326133128 1104 70-71 -0.4725294426568709 1104 72-73 -0.2690191087317828 1104 74-75 -0.25857991843331973 1104 76-77 -0.31481424943742553 1104 78-79 -0.21591674319698484 1104 80-81 -0.4523368661401861 1104 82-83 -0.39841019839487046 1104 84-85 -0.22902850768233662 1104 86-87 -0.3289633573348354 1104 88-89 -0.17759581853830042 1104 90-91 -0.16074022271005362 1104 92-93 -0.28047052654262217 1104 94-95 -0.23648018592749764 1104 96-97 -0.34355739712602684 1104 98-99 -0.4952954696644838 1104 100 -0.46394974840600867 1105 1 1.451979708042483 1105 2 0.19505308758261286 1105 3 -0.08210132555030114 1105 4 -0.23188341310533112 1105 5 -1.1167188797562666 1105 6 0.5819073856488046 1105 7 -0.01818120702176884 1105 8 -0.09633787103320657 1105 9 -0.033013607118057564 1105 10-11 -0.10751779420525764 1105 12-13 -0.1197355972508376 1105 14-15 -0.018931379828678985 1105 16-17 -0.07475820080728113 1105 18-19 -0.057102769226695216 1105 20-21 -0.07811609142728315 1105 22-23 -0.0951574028731983 1105 24-25 -0.0917693552056491 1105 26-27 -0.08819937955917112 1105 28-29 -0.12663726502537997 1105 30-31 -0.22519866645627218 1105 32-33 -0.09187640493495053 1105 34-35 -0.16056918474364323 1105 36-37 -0.277949885486386 1105 38-39 -0.2906418432738107 1105 40-41 -0.3209992874200438 1105 42-43 -0.15756860632415481 1105 44-45 -0.035091734643863504 1105 46-47 -0.3584958727427008 1105 48-49 -0.4392456808868417 1105 50-51 -0.3510242256946938 1105 52-53 -0.17201463554826546 1105 54-55 -0.0998676823023672 1105 56-57 -0.18398888156669813 1105 58-59 -0.309034468873854 1105 60-61 -0.224381508253785 1105 62-63 -0.40240989016337636 1105 64-65 -0.3724215989407824 1105 66-67 -0.4679988334354164 1105 68-69 -0.5415489287704034 1105 70-71 -0.48729563663746944 1105 72-73 -0.509949754736077 1105 74-75 -0.6600750408777074 1105 76-77 -0.5695344337811719 1105 78-79 -0.3017060221122918 1105 80-81 -0.5326973131127417 1105 82-83 -0.45348060534743695 1105 84-85 -0.557871514103077 1105 86-87 -0.5061576851019964 1105 88-89 -0.7156300701568341 1105 90-91 -0.5028768095274927 1105 92-93 -0.5492644712776453 1105 94-95 -0.4957345703864675 1105 96-97 -0.3123635500565669 1105 98-99 -0.636309665506829 1105 100 -0.9686823316983322 1106 1 1.225103609781769 1106 2 -0.4962260947734922 1106 3 -0.21858473957217583 1106 4 -0.0714028680094021 1106 5 -1.0759241872692655 1106 6 -0.6595689636429292 1106 7 0.010064516485499553 1106 8 0.02102357777439323 1106 9 -0.10004233223909154 1106 10-11 -0.12351435149412993 1106 12-13 -0.004389568920196041 1106 14-15 -0.056879237099614954 1106 16-17 -0.08578864728388425 1106 18-19 -0.10038008678093746 1106 20-21 -0.04119789167831556 1106 22-23 -0.07595023055833394 1106 24-25 -0.1305550822990682 1106 26-27 -0.15867755779119364 1106 28-29 -0.06949586283782594 1106 30-31 0.02339032404041319 1106 32-33 -0.26207773181319993 1106 34-35 -0.16025016017846383 1106 36-37 -0.41239880067376333 1106 38-39 -0.3079845006164632 1106 40-41 -0.07094413231873631 1106 42-43 0.007724275153343285 1106 44-45 -0.11266218470662892 1106 46-47 -0.41182920607603535 1106 48-49 -0.23386816609394145 1106 50-51 -0.13470921762583288 1106 52-53 -0.1880908055321271 1106 54-55 -0.3056158623274712 1106 56-57 -0.09634333736730838 1106 58-59 -0.20937501038362427 1106 60-61 -0.2464630574737896 1106 62-63 -0.31505238612894715 1106 64-65 -0.3615538205653124 1106 66-67 -0.25710193546160554 1106 68-69 -0.32308523861440364 1106 70-71 -0.6343328250900413 1106 72-73 -0.28359106726969685 1106 74-75 -0.4637888655146085 1106 76-77 -0.31600486591314336 1106 78-79 -0.3047835189743964 1106 80-81 -0.4005293374986181 1106 82-83 -0.3440263467854905 1106 84-85 -0.6307803898427267 1106 86-87 -0.3325526644815895 1106 88-89 -0.44833711695618916 1106 90-91 -0.4725215647722436 1106 92-93 -0.41761706227639195 1106 94-95 -0.34230713628210196 1106 96-97 -0.27339220345445625 1106 98-99 -0.5343540621358471 1106 100 -0.46978586406340384 1107 1 1.198894708782678 1107 2 -0.3748527977707674 1107 3 -0.15629227817344749 1107 4 -0.31845100606571464 1107 5 -1.3331957585680847 1107 6 -0.4785001171397525 1107 7 -0.5513916143046913 1107 8 -0.26804308889227 1107 9 -0.21556494804290338 1107 10-11 -0.27646040054045073 1107 12-13 -0.3619212673761467 1107 14-15 -0.3651284650741857 1107 16-17 -0.1959990015073103 1107 18-19 -0.208891621749153 1107 20-21 -0.24096755562018046 1107 22-23 -0.10307030321955324 1107 24-25 -0.352349814360835 1107 26-27 -0.2789514905795656 1107 28-29 -0.25413600816025905 1107 30-31 -0.4231041355417915 1107 32-33 -0.3440882676896777 1107 34-35 -0.3771344471902651 1107 36-37 -0.2859130604376219 1107 38-39 -0.3809545278644251 1107 40-41 -0.3817786463968531 1107 42-43 -0.2872068783434756 1107 44-45 -0.17546400123705297 1107 46-47 -0.35086644494978003 1107 48-49 -0.6025286565571548 1107 50-51 -0.3757577189882326 1107 52-53 -0.22925483822967863 1107 54-55 -0.5910574608742465 1107 56-57 -0.3012930194744783 1107 58-59 -0.5224089795026075 1107 60-61 -0.36192681769177426 1107 62-63 -0.35714720901723496 1107 64-65 -0.6315854281947466 1107 66-67 -0.7186241879593283 1107 68-69 -0.693690143246549 1107 70-71 -0.7364552592408131 1107 72-73 -0.41451858770566474 1107 74-75 -0.46037742137291815 1107 76-77 -0.750761632488981 1107 78-79 -0.8986240276029207 1107 80-81 -0.7518920986248645 1107 82-83 -0.7055733040970225 1107 84-85 -0.40552480401166235 1107 86-87 -0.14744603414553836 1107 88-89 -0.28498234856381544 1107 90-91 -0.19763110155698627 1107 92-93 -0.3329202412046399 1107 94-95 -0.25854909813497073 1107 96-97 -0.3531575077233029 1107 98-99 -0.3165549704010573 1107 100 -0.5594977623376991 1108 1 1.4456075410306273 1108 2 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0.25409410563075596 2223 36-37 0.19123078131979554 2223 38-39 0.2578032807340165 2223 40-41 0.1944573682171722 2223 42-43 0.3748693984116471 2223 44-45 0.2413481046824444 2223 46-47 0.4375920157889155 2223 48-49 0.27667674280209553 2223 50-51 0.2374717041319343 2223 52-53 0.2900198054003482 2223 54-55 0.31681757818699907 2223 56-57 0.49824744505498586 2223 58-59 0.47990344620801295 2223 60-61 0.5566400507791514 2223 62-63 0.3816558668185337 2223 64-65 0.3427994484593384 2223 66-67 0.4763514407441889 2223 68-69 0.5981494880737017 2223 70-71 0.6614395221768419 2223 72-73 0.7143086111868939 2223 74-75 0.46386172398164405 2223 76-77 0.6404928832829953 2223 78-79 0.7495603181102766 2223 80-81 0.5784597300255001 2223 82-83 0.5398904806528861 2223 84-85 0.2959625897928575 2223 86-87 0.4744346881443491 2223 88-89 0.6320796033010438 2223 90-91 0.6518462808869216 2223 92-93 0.6754264532648762 2223 94-95 0.586836272078024 2223 96-97 0.6154901116580902 2223 98-99 0.6277299678748705 2223 100 0.6210154221102329 2224 1 1.3447649854431454 2224 2 -0.6726917032391011 2224 3 -0.06897627396370964 2224 4 -0.2777520743586077 2224 5 -1.0573210126660904 2224 6 0.5913051104311435 2224 7 0.3589280085489861 2224 8 0.19015796930878537 2224 9 0.27548359368683606 2224 10-11 0.1624623680825934 2224 12-13 0.19876598663536527 2224 14-15 0.23087949305911337 2224 16-17 0.24398701409177903 2224 18-19 0.23175324655239393 2224 20-21 0.14631745223702808 2224 22-23 0.30125188584378293 2224 24-25 0.39407983833585547 2224 26-27 0.26358487607124914 2224 28-29 0.13493165038968868 2224 30-31 0.2639956150456939 2224 32-33 0.27892544278997633 2224 34-35 0.13131386098555708 2224 36-37 0.026866807791840586 2224 38-39 0.025295922663964632 2224 40-41 0.22972676715215812 2224 42-43 0.4013814180104802 2224 44-45 0.41724681000237496 2224 46-47 0.23832952408269392 2224 48-49 0.32320696618119626 2224 50-51 0.2976632691466534 2224 52-53 0.3577166018752749 2224 54-55 0.24265503714342884 2224 56-57 0.3786069271829575 2224 58-59 0.5222884816798654 2224 60-61 0.6120110166002775 2224 62-63 0.5928333281567646 2224 64-65 0.4953922111807145 2224 66-67 0.4222419798823154 2224 68-69 0.3562586767295173 2224 70-71 0.5384968045395908 2224 72-73 0.3563729538943221 2224 74-75 0.24988309215734716 2224 76-77 0.6031972504889751 2224 78-79 0.4125836767927993 2224 80-81 0.2787532021839283 2224 82-83 0.5275884680293288 2224 84-85 0.34831802285568614 2224 86-87 0.6203235259945998 2224 88-89 0.4436755814565103 2224 90-91 0.49399483734415384 2224 92-93 0.5437416678823368 2224 94-95 0.6973034457284726 2224 96-97 0.5562776378153913 2224 98-99 0.5885305939488177 2224 100 0.8257739242963851 2225 1 1.4794929934032108 2225 2 1.1940242981023594 2225 3 0.9624468651074114 2225 4 1.1063516282364514 2225 5 3.1765793922662695 2225 6 0.012551448440184743 2225 7 0.15114948243625292 2225 8 0.19146450670751847 2225 9 0.18392120888191243 2225 10-11 0.16383902723818977 2225 12-13 0.2045077944463145 2225 14-15 0.1422259139393205 2225 16-17 0.18944739252754061 2225 18-19 0.05269789471024211 2225 20-21 0.16190097334700937 2225 22-23 0.11158278847432257 2225 24-25 0.11117846056805547 2225 26-27 0.04418900410858129 2225 28-29 -0.06233637270337766 2225 30-31 0.11245252065294409 2225 32-33 0.07102385715135284 2225 34-35 0.1172565274415831 2225 36-37 -0.017263197391066853 2225 38-39 0.012204079792127231 2225 40-41 0.06806798571862771 2225 42-43 0.429722109970875 2225 44-45 0.4214552240588674 2225 46-47 0.284510937105388 2225 48-49 0.17574063432513043 2225 50-51 0.0838085403626394 2225 52-53 0.1729912269456051 2225 54-55 0.09516597807763105 2225 56-57 0.09402872479438429 2225 58-59 0.02497791435882135 2225 60-61 0.18374626677974248 2225 62-63 0.29326736243050533 2225 64-65 0.07902072107952307 2225 66-67 0.1330548656559145 2225 68-69 0.19712219983504298 2225 70-71 0.13378423445247734 2225 72-73 0.29191401392463945 2225 74-75 0.2302415168845542 2225 76-77 0.49785677194262945 2225 78-79 0.09724253305981989 2225 80-81 0.04269385090717748 2225 82-83 0.037690712757033396 2225 84-85 -0.09497232279191081 2225 86-87 0.10985431997799111 2225 88-89 0.013047063242872525 2225 90-91 0.06927497792026571 2225 92-93 0.26074550102027416 2225 94-95 0.2609712293543538 2225 96-97 0.31520859975839954 2225 98-99 0.23418332218403748 2225 100 0.4419889569598183 2226 1 1.313739418895512 2226 2 1.4960537760553567 2226 3 0.7826021997891441 2226 4 1.0821672791021886 2226 5 3.156306885568913 2226 6 -0.23709260913987862 2226 7 0.02262339700217808 2226 8 0.43723139980524905 2226 9 0.4494500251344249 2226 10-11 0.2970837109386011 2226 12-13 0.42919894919964463 2226 14-15 0.4627408561906634 2226 16-17 0.45204084679577505 2226 18-19 0.2993169351063969 2226 20-21 0.30817892927611723 2226 22-23 0.3010679251646735 2226 24-25 0.4936458506036985 2226 26-27 0.32416090650733764 2226 28-29 0.46113721932937324 2226 30-31 0.4068618705061411 2226 32-33 0.2851798211110932 2226 34-35 0.2818218886195254 2226 36-37 0.430223947801295 2226 38-39 0.25546006343089545 2226 40-41 0.475645175180361 2226 42-43 0.5677097792796459 2226 44-45 0.3434384252682605 2226 46-47 0.4544750637481485 2226 48-49 0.38172838934201536 2226 50-51 0.15319433458083864 2226 52-53 0.48674622353137664 2226 54-55 0.4533743063126394 2226 56-57 0.6443939428623295 2226 58-59 0.6699630592252745 2226 60-61 0.6325009898889675 2226 62-63 0.5448016504695445 2226 64-65 0.5228496239987308 2226 66-67 0.5094994280116651 2226 68-69 0.5085681521283334 2226 70-71 0.5434563388855622 2226 72-73 0.3767589865516143 2226 74-75 0.5994770117727981 2226 76-77 0.9122884961248943 2226 78-79 0.6646815689337444 2226 80-81 0.5633799556481378 2226 82-83 0.3905813317218687 2226 84-85 0.4690964669925819 2226 86-87 0.5786273857516377 2226 88-89 0.5221197183505879 2226 90-91 0.5968299960458339 2226 92-93 0.5360981870956891 2226 94-95 0.7841303233519099 2226 96-97 0.5641645959714552 2226 98-99 0.5825356400528889 2226 100 0.8648271248135231 2227 1 1.5682867033708305 2227 2 1.531937996862096 2227 3 0.4031705436812434 2227 4 -0.04920432423387311 2227 5 3.1336414316261347 2227 6 0.07345550803172785 2227 7 0.3890275425909806 2227 8 0.46056639432562463 2227 9 0.0029407258322962093 2227 10-11 0.12210781153198269 2227 12-13 0.0978632458551516 2227 14-15 0.22877972223265175 2227 16-17 0.07587187482587154 2227 18-19 0.06244939288519191 2227 20-21 0.21050901650850307 2227 22-23 0.16623851034026416 2227 24-25 0.2731074998355467 2227 26-27 0.2261440369521921 2227 28-29 0.2582695784692248 2227 30-31 0.23983457905728045 2227 32-33 0.21139028452493136 2227 34-35 0.17942705523621072 2227 36-37 0.12553137158693062 2227 38-39 0.18920249991629845 2227 40-41 0.33138355370505934 2227 42-43 0.46245785711036547 2227 44-45 0.20435276823246085 2227 46-47 0.3210459493137705 2227 48-49 0.317197150901265 2227 50-51 0.1686425138605827 2227 52-53 0.26986167182535326 2227 54-55 0.3193517810751274 2227 56-57 0.32403712791417405 2227 58-59 0.4660889569401192 2227 60-61 0.532369547747308 2227 62-63 0.4910143160553915 2227 64-65 0.4968108038353236 2227 66-67 0.39457343303422476 2227 68-69 0.42375972675586837 2227 70-71 0.6355231862756128 2227 72-73 0.44871452676333945 2227 74-75 0.39748550848645436 2227 76-77 0.6360670218510194 2227 78-79 0.4269212581522268 2227 80-81 0.4293654591627245 2227 82-83 0.44353195546988644 2227 84-85 0.5053052787774064 2227 86-87 0.4642461989529707 2227 88-89 0.42192777382697244 2227 90-91 0.4904133313385657 2227 92-93 0.5301874484677 2227 94-95 0.6628687827376112 2227 96-97 0.5373395315837328 2227 98-99 0.8042537872692321 2227 100 0.7223724026742957 2228 1 1.6572065036724446 2228 2 1.0467063811107522 2228 3 -0.10961153485620656 2228 4 -0.09283909523341016 2228 5 3.1866181492795036 2228 6 0.25978194739672844 2228 7 0.3983757705047921 2228 8 0.41244994433387916 2228 9 0.47774630656047634 2228 10-11 0.4287913291060832 2228 12-13 0.4344215779179592 2228 14-15 0.4713531316034931 2228 16-17 0.3759125317086145 2228 18-19 0.22687650485892874 2228 20-21 0.2728201147525553 2228 22-23 0.33179510706224846 2228 24-25 0.49938275264198495 2228 26-27 0.3085541678251005 2228 28-29 0.3165058520603452 2228 30-31 0.41489814826334026 2228 32-33 0.23127832392098924 2228 34-35 0.4001284035943158 2228 36-37 0.3533422497013632 2228 38-39 0.28352675340283184 2228 40-41 0.21026658579487645 2228 42-43 0.633175936460951 2228 44-45 0.5658389942858761 2228 46-47 0.43652020442771544 2228 48-49 0.575444159736719 2228 50-51 0.34224126468927807 2228 52-53 0.5073295588837325 2228 54-55 0.37630310873362305 2228 56-57 0.7067147548084662 2228 58-59 0.6414790089089806 2228 60-61 0.7195553776816155 2228 62-63 0.775814494899997 2228 64-65 0.5831531462085309 2228 66-67 0.678387882330469 2228 68-69 0.6501323390919183 2228 70-71 0.6107964353601147 2228 72-73 0.9337619873926855 2228 74-75 0.7438724420952525 2228 76-77 0.6190566560589943 2228 78-79 0.7876096214328854 2228 80-81 0.7579881330265721 2228 82-83 0.6599333531073412 2228 84-85 0.4500354729654177 2228 86-87 0.3656458349825016 2228 88-89 0.44772804917457165 2228 90-91 0.6628323473392257 2228 92-93 0.7694779002102052 2228 94-95 0.9951964006953844 2228 96-97 0.6173657815401938 2228 98-99 1.0050075670174223 2228 100 0.9516692270405613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 18.0 13 34.0 14 103.0 15 352.0 16 1364.0 17 3407.0 18 5766.0 19 7281.0 20 8587.0 21 10049.0 22 11664.0 23 13317.0 24 15227.0 25 17101.0 26 19407.0 27 21901.0 28 24654.0 29 28031.0 30 31663.0 31 36780.0 32 43211.0 33 51226.0 34 63205.0 35 81534.0 36 114460.0 37 189164.0 38 466468.0 39 807694.0 40 561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.528333099647078 26.06302789996489 18.33366484174006 33.07497415864797 2 31.72965058114335 43.792226125921545 9.821026073465083 14.657097219470025 3 30.783148823008453 20.04826853736979 25.582903334202733 23.585679305419024 4 19.387444684265816 17.666178613836756 23.429187423375144 39.51718927852229 5 17.91224594777144 35.50123925564632 26.53506435403227 20.051450442549978 6 17.37344333725929 27.713960223292606 31.244525074135982 23.668071365312123 7 20.625832538258795 26.836429343143887 36.151215704726916 16.38652241387041 8 20.553323668698102 27.802426829438794 34.49527511186084 17.148974390002262 9 20.814239893473673 28.05403839209653 27.99290724408925 23.138814470340545 10-11 19.56842759406025 30.149636322701113 28.88229795263686 21.39963813060178 12-13 24.233293430956756 26.672320172941365 27.30291592683354 21.79147046926834 14-15 21.745935477712443 27.346498385665228 28.64987424242936 22.257691894192973 16-17 21.028488175606455 29.52875502174543 28.201611297498975 21.24114550514914 18-19 21.428950226807164 29.565636195424904 29.802856868744964 19.202556709022968 20-21 22.711282119765947 29.050553241710535 29.855575252298568 18.382589386224954 22-23 21.98076972214736 30.42301500943242 28.50649566658262 19.089719601837597 24-25 22.118700490640137 28.397057412657905 26.67388702018919 22.810355076512767 26-27 20.17964265276399 26.38361048852369 30.135317749390257 23.301429109322065 28-29 19.816553214681818 28.02535211335508 30.67704065927258 21.48105401269052 30-31 20.04245907780966 30.1384717528521 28.233526856596225 21.585542312742014 32-33 20.619227674475674 30.15532518347781 28.79759177988544 20.427855362161075 34-35 21.448571975418336 29.274202607330242 29.874955947486992 19.402269469764427 36-37 21.89615997076504 28.22205262774747 28.754901218717894 21.126886182769596 38-39 21.55473173196362 29.37910865544292 28.854473536468333 20.211686076125122 40-41 21.00154252948319 29.719642434000153 29.772530536611754 19.5062844999049 42-43 20.569889684152066 29.929380887135732 29.281139085133447 20.219590343578762 44-45 20.573138260047468 30.108247450016368 28.916117747847515 20.40249654208865 46-47 19.876973454412127 28.048104642470673 29.0053473976424 23.069574505474797 48-49 20.769071488507652 28.327375696554164 30.247728251733115 20.655824563205066 50-51 21.906935058761594 30.54559067489655 28.129271165334202 19.418203101007652 52-53 20.36566886783766 30.409714786286173 28.298008050336538 20.92660829553963 54-55 22.61312651126883 28.18481860916373 28.19115963941478 21.010895240152657 56-57 21.652712049508555 28.680872812484182 29.300477100846923 20.365938037160348 58-59 21.30870606297018 27.9887643483351 28.91555372543196 21.786975863262764 60-61 19.378978289261497 30.548560981994736 29.606041190284383 20.466419538459387 62-63 20.386076277428135 31.28353365630918 27.409583438306512 20.920806627956175 64-65 19.767060098562066 30.32094863336975 28.889257968996628 21.022733299071557 66-67 20.18171571219957 30.221518453362673 29.52412679602879 20.072639038408973 68-69 20.8300771033478 30.013007242691142 29.287074860104646 19.869840793856415 70-71 19.63867056144717 30.85049914932247 29.297608894678458 20.2132213945519 72-73 20.014906743662344 30.70114244859174 28.578594745324647 20.705356062421266 74-75 19.98246095296132 29.172839643067377 30.49841652006601 20.346282883905296 76-77 19.606827404302997 30.467826840720093 28.605303464564425 21.320042290412484 78-79 19.45424058770753 29.497201128708543 28.49950511732311 22.549053166260812 80-81 21.15193812053267 29.096998109174216 28.270448832480167 21.48061493781295 82-83 20.47384835522018 27.920085005079 29.989070637812894 21.61699600188793 84-85 20.786952646019316 29.166837412841108 28.39259792433719 21.653612016802388 86-87 21.12654870797776 29.540060427272014 29.293366354438206 20.040024510312023 88-89 21.299528644137173 29.364260166066526 28.94145246257766 20.394758727218644 90-91 21.776115366239697 29.464200915135212 28.353330321772575 20.406353396852516 92-93 21.967945680057504 29.652053847477788 28.734194729704388 19.645805742760324 94-95 22.555489292648016 29.008537630126664 28.29752645440788 20.13844662281744 96-97 21.528818659849033 29.481243392123048 28.383365578246185 20.606572369781738 98-99 22.938330338646313 30.13023152217041 27.75236003353535 19.17907810564793 100 22.06410189038915 31.699874989694965 26.631100037652544 19.604923082263337 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 7.0 2 10.0 3 16.0 4 24.0 5 26.0 6 29.0 7 39.5 8 47.5 9 59.5 10 74.0 11 78.0 12 86.0 13 95.5 14 116.5 15 151.0 16 201.0 17 258.5 18 373.0 19 604.5 20 966.5 21 1448.0 22 2246.5 23 3551.0 24 5442.5 25 8016.5 26 11414.5 27 15567.5 28 20017.0 29 25012.5 30 31052.0 31 37732.0 32 45171.0 33 53731.0 34 63076.5 35 73162.5 36 82369.5 37 89393.5 38 94390.0 39 97693.5 40 99895.5 41 100233.0 42 98452.0 43 96716.0 44 96251.5 45 93682.0 46 86845.5 47 79143.5 48 73577.5 49 71018.5 50 69175.5 51 63577.0 52 54996.0 53 46912.0 54 39195.0 55 31332.5 56 24376.0 57 18150.5 58 12758.5 59 9214.0 60 7225.0 61 5929.5 62 4873.5 63 3829.0 64 3019.5 65 2879.5 66 3329.5 67 3061.5 68 1997.5 69 1701.5 70 2129.0 71 2003.5 72 1212.0 73 551.0 74 257.0 75 150.5 76 98.5 77 80.0 78 70.5 79 64.5 80 50.5 81 37.5 82 27.5 83 22.5 84 17.5 85 13.5 86 11.0 87 14.0 88 13.0 89 11.0 90 11.0 91 11.0 92 11.5 93 11.5 94 12.0 95 8.5 96 7.5 97 11.0 98 35.0 99 70.0 100 73.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.288772840414438 2 0.0061709676221863635 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.687373959191584E-4 30-31 0.011184878815212785 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0012293724559824398 40-41 0.0018561113551107424 42-43 1.4463205364499291E-4 44-45 0.0 46-47 0.0020489540933040663 48-49 2.4105342274165482E-5 50-51 0.0 52-53 0.0014463205364499293 54-55 0.020899331751701476 56-57 0.011763407029792757 58-59 0.014222151941757636 60-61 9.642136909666193E-5 62-63 0.013812361123096825 64-65 1.4463205364499291E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4463205364499291E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2074229.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.57346632799084 #Duplication Level Percentage of deduplicated Percentage of total 1 89.36930812020651 72.00794938579297 2 7.167453171443612 11.55013093533526 3 1.64061909935923 3.9657110327783873 4 0.6521768699187497 2.1019260427317135 5 0.33884265142057984 1.3650863482361613 6 0.19050717448868165 0.9209894045342679 7 0.13120210615017583 0.739998593743687 8 0.09212507035849449 0.5938269003595154 9 0.06420976239403359 0.4656242815365561 >10 0.33362847008277174 4.6565951385997915 >50 0.014296824224198612 0.7841452681145314 >100 0.0054492954527619675 0.7642252748225363 >500 1.8138450022313403E-4 0.08379139341458149 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 4.821068454833097E-5 0.0 0.0 0.0 0.0 36-37 4.821068454833097E-5 0.0 0.0 0.0 0.0 38-39 4.821068454833097E-5 0.0 0.0 0.0 0.0 40-41 4.821068454833097E-5 0.0 0.0 0.0 0.0 42-43 4.821068454833097E-5 0.0 0.0 0.0 0.0 44-45 4.821068454833097E-5 0.0 0.0 0.0 0.0 46-47 4.821068454833097E-5 0.0 0.0 0.0 0.0 48-49 4.821068454833097E-5 0.0 0.0 0.0 0.0 50-51 4.821068454833097E-5 0.0 0.0 0.0 0.0 52-53 4.821068454833097E-5 0.0 0.0 0.0 0.0 54-55 9.642136909666194E-5 0.0 0.0 0.0 0.0 56-57 1.4463205364499291E-4 0.0 0.0 0.0 0.0 58-59 2.1694808046748937E-4 0.0 0.0 0.0 0.0 60-61 2.6515876501582037E-4 0.0 0.0 0.0 0.0 62-63 3.133694495641513E-4 0.0 0.0 0.0 0.0 64-65 3.374747918383168E-4 0.0 0.0 0.0 0.0 66-67 3.8568547638664777E-4 0.0 0.0 0.0 0.0 68-69 4.3389616093497874E-4 0.0 0.0 2.4105342274165486E-5 0.0 70-71 5.544228723058061E-4 0.0 0.0 4.821068454833097E-5 0.0 72-73 6.749495836766336E-4 0.0 0.0 4.821068454833097E-5 0.0 74-75 7.231602682249646E-4 0.0 0.0 4.821068454833097E-5 0.0 76-77 8.43686979595792E-4 0.0 0.0 4.821068454833097E-5 0.0 78-79 8.91897664144123E-4 0.0 0.0 4.821068454833097E-5 0.0 80-81 0.0010124243755149503 0.0 0.0 4.821068454833097E-5 0.0 82-83 0.0013498991673532672 0.0 0.0 4.821068454833097E-5 0.0 84-85 0.0015186365632724255 0.0 0.0 4.821068454833097E-5 0.0 86-87 0.001542741905546591 4.821068454833097E-5 0.0 4.821068454833097E-5 0.0 88 0.001542741905546591 4.821068454833097E-5 0.0 4.821068454833097E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTGC 40 1.0459189E-9 82.150955 2 TGCTGCG 40 4.276626E-8 79.40675 1 TCACCGG 8545 0.0 78.881485 8 CACCGGC 8390 0.0 76.19875 9 GAGCTGC 35 1.7952643E-6 75.62548 1 CTTCACC 9310 0.0 74.46695 6 TGCTGCA 65 3.6379788E-12 73.298546 1 GTGTGGC 3175 0.0 71.849754 7 CCTTCAC 10025 0.0 71.73104 5 GCAGCCA 90 0.0 67.807144 2 GGCGCGC 340 0.0 66.266655 6 TTCACCG 10390 0.0 66.22945 7 GGCTGCG 40 3.967978E-6 66.172295 1 GGCTGCA 65 2.564775E-10 65.15426 1 TGCTGCC 65 1.0913936E-11 64.992294 3 CGTAGTG 1640 0.0 64.68407 4 CAGCGTA 550 0.0 64.487915 1 AGCTGCG 50 2.0058906E-7 63.525406 1 CGCAGCC 25 0.0032691897 63.525406 1 CGGGCCC 585 0.0 61.782803 7 >>END_MODULE