FastQCFastQC Report
Mon 6 Mar 2017
1HL_22A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_22A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2074229
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTGC401.0459189E-982.1509552
TGCTGCG404.276626E-879.406751
TCACCGG85450.078.8814858
CACCGGC83900.076.198759
GAGCTGC351.7952643E-675.625481
CTTCACC93100.074.466956
TGCTGCA653.6379788E-1273.2985461
GTGTGGC31750.071.8497547
CCTTCAC100250.071.731045
GCAGCCA900.067.8071442
GGCGCGC3400.066.2666556
TTCACCG103900.066.229457
GGCTGCG403.967978E-666.1722951
GGCTGCA652.564775E-1065.154261
TGCTGCC651.0913936E-1164.9922943
CGTAGTG16400.064.684074
CAGCGTA5500.064.4879151
AGCTGCG502.0058906E-763.5254061
CGCAGCC250.003269189763.5254061
CGGGCCC5850.061.7828037
Produced by FastQC (version 0.11.5)