FastQCFastQC Report
Tue 7 Mar 2017
1HL_23A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_23A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3609794
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA172980.47919631978999355No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG94190.26092901700207827No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG71820.19895872174423251No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA71620.19840467350768493No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT67000.18560615924343604No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61760.1710900954458897No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC59140.16383206354711655No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG57560.15945508247839074No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG56410.1562693051182422No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA55410.15349906393550436No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT52150.14446807767977896No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA49650.13754247472293435No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT47330.1311155151789825No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA45050.1247993652823402No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA44590.1235250543382808No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC43790.12130886139209052No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA42020.11640553449864452No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA40920.11335826919763288No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38000.10526916494403836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCC600.086.000012
AGCTGCC2950.086.000012
GCTGCCT500.086.03
GCTGCCC1950.086.03
GCAGCTT402.1827873E-1186.03
GCAGCCA900.086.03
CAGCTGC7200.085.402781
AGCAGCA3100.084.612912
GCAGCAA1850.083.675673
CAGCAGC7300.083.643841
AGCTGCA2150.082.02
CTGCAGC2050.081.804881
GCAGCAG950.081.473693
GCTGCAT750.080.266673
CAGCCCT194900.080.2637251
CTGCTGC3550.079.9436651
GCTGCAA1350.079.629633
AGCTGCT1350.079.629632
AGCAGCC1850.079.027022
AGCCCTT168500.078.420772
Produced by FastQC (version 0.11.5)