FastQCFastQC Report
Tue 7 Mar 2017
1HL_23A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_23A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3609794
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG142400.081.0228358
CACCGGC142700.078.484169
CTTCACC162600.073.43996
CCTTCAC179350.070.297555
TTCACCG172250.069.1893167
CGTAGTG28400.067.4337544
GTGTGGC54500.066.920647
CGGGCCC10500.066.16197
GGGCCCG10600.065.537738
AACGGGC10700.064.486545
TAGCTGC751.2732926E-1163.518681
GGCGCGC4800.062.5855876
CAGCTGC1700.062.2732161
ACCTTCA70950.061.262424
ACGGGCC11350.061.2070436
GGCCCGA11350.061.2070439
GCGTAGT19200.061.1187363
CAGCGTA13150.058.3663441
GCCTTCA52250.057.4050564
TGTGGCA64950.057.165358
Produced by FastQC (version 0.11.5)