FastQCFastQC Report
Mon 6 Mar 2017
1HL_24A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_24A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2721808
Sequences flagged as poor quality0
Sequence length92
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA144250.5299786024583659No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG87840.3227266581625155No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA62250.22870827038497943No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT56490.20754586657104396No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG55890.20534144950709235No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG53130.19520113101291495No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG50940.18715500872949156No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC50740.1864202030415077No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA47210.1734508826485924No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45680.1678296191355158No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45680.1678296191355158No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA44810.16463321439278597No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA43510.1598569774208908No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42740.15702797552215292No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC40430.14854096982593923No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34970.12848077454397958No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA34100.12528436980124977No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT32420.11911200202218526No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG31350.11518079159147156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC5650.085.999751
GCTGCCG159.829813E-485.999753
GCTGCAC302.4598194E-885.999753
GCTGCAA650.085.999753
TGCTGCG159.829813E-485.999752
TGCTGCC302.4598194E-885.999752
GCAGCTC159.829813E-485.999753
GCAGCAT1100.085.999753
GCTGCTC202.8585835E-585.999743
GCTGCCT402.1827873E-1185.999743
GCTGCCC1600.085.999743
GCTGCAT800.085.999743
GCAGCTT500.085.999743
GCAGCCT258.3594205E-785.999743
GCAGCAC402.1827873E-1185.999743
TGCAGCA258.3594205E-785.999742
AGCTGCC2200.084.045212
CAGCTGC6300.083.9521261
AGCTGCA1600.083.312252
AGCAGCC1450.083.034242
Produced by FastQC (version 0.11.5)