FastQCFastQC Report
Tue 7 Mar 2017
1HL_24A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_24A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2721808
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG105500.080.618138
CACCGGC104100.078.36579
CTTCACC120250.074.6328056
CGTAGTG25750.074.007514
CCTTCAC133400.071.674085
CGGGCCC7750.069.044667
CCGCGTA3000.068.828641
GTGTGGC42700.068.7035457
TTCACCG131550.067.401277
GCGTAGT17950.066.681093
CGGGGCA11500.066.5298894
CAGCGTA12200.065.096441
AACGGGC8450.063.3249865
GGCCCGA8250.062.5843479
GCCTTCA41850.062.2478684
GGGCCCG8550.062.0353558
GCAGCCT609.98989E-961.7692951
TGTGGCA46650.060.773228
TAGTGTG28800.058.8358046
ACCTTCA49750.058.590274
Produced by FastQC (version 0.11.5)