##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1HL_24A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2721808
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.798297308259803	12.0	12.0	12.0	2.0	32.0
2	14.60334307195805	12.0	12.0	12.0	12.0	22.0
3	13.69370322961796	12.0	12.0	12.0	12.0	22.0
4	13.694232289713309	12.0	12.0	12.0	12.0	22.0
5	22.435181320651566	22.0	22.0	22.0	22.0	27.0
6	26.76207763369055	27.0	22.0	32.0	22.0	32.0
7	34.927016527249535	37.0	37.0	37.0	27.0	41.0
8	37.56082758225415	41.0	37.0	41.0	27.0	41.0
9	38.50806339021709	41.0	37.0	41.0	32.0	41.0
10-11	38.873517713225915	41.0	41.0	41.0	37.0	41.0
12-13	39.04878742365369	41.0	41.0	41.0	37.0	41.0
14-15	39.003975115070574	41.0	41.0	41.0	37.0	41.0
16-17	38.91131924073998	41.0	41.0	41.0	37.0	41.0
18-19	38.851244099510325	41.0	41.0	41.0	37.0	41.0
20-21	38.85963741748132	41.0	41.0	41.0	37.0	41.0
22-23	38.84440618147937	41.0	41.0	41.0	34.5	41.0
24-25	38.869072138813614	41.0	41.0	41.0	37.0	41.0
26-27	38.83781552556242	41.0	41.0	41.0	37.0	41.0
28-29	38.78582986015178	41.0	41.0	41.0	34.5	41.0
30-31	38.657453795418334	41.0	41.0	41.0	32.0	41.0
32-33	38.706805182437556	41.0	41.0	41.0	32.0	41.0
34-35	38.677804606349895	41.0	41.0	41.0	32.0	41.0
36-37	38.59919950268351	41.0	41.0	41.0	32.0	41.0
38-39	38.500091483308154	41.0	41.0	41.0	32.0	41.0
40-41	38.5457291623803	41.0	41.0	41.0	32.0	41.0
42-43	38.28210402791086	41.0	41.0	41.0	32.0	41.0
44-45	38.39012524028146	41.0	41.0	41.0	32.0	41.0
46-47	38.40959152886611	41.0	41.0	41.0	32.0	41.0
48-49	38.3487417187399	41.0	41.0	41.0	32.0	41.0
50-51	38.22881720532823	41.0	41.0	41.0	32.0	41.0
52-53	38.2081509790551	41.0	41.0	41.0	32.0	41.0
54-55	38.08238531152822	41.0	37.0	41.0	32.0	41.0
56-57	37.87146870756497	41.0	37.0	41.0	27.0	41.0
58-59	37.86889266252432	41.0	37.0	41.0	29.5	41.0
60-61	37.85241482867271	41.0	37.0	41.0	29.5	41.0
62-63	37.8623999194653	41.0	37.0	41.0	29.5	41.0
64-65	37.852658416758274	41.0	37.0	41.0	29.5	41.0
66-67	37.70980392445022	41.0	37.0	41.0	27.0	41.0
68-69	37.75241512259498	41.0	37.0	41.0	27.0	41.0
70-71	37.72468722995891	41.0	37.0	41.0	27.0	41.0
72-73	37.56541993410262	41.0	37.0	41.0	27.0	41.0
74-75	37.504155326165545	41.0	37.0	41.0	27.0	41.0
76-77	36.42463061318065	39.0	34.5	41.0	24.5	41.0
78-79	36.961291722266964	41.0	37.0	41.0	27.0	41.0
80-81	37.60799567787294	41.0	37.0	41.0	27.0	41.0
82-83	37.79640702062746	41.0	37.0	41.0	27.0	41.0
84-85	37.71250415165214	41.0	37.0	41.0	27.0	41.0
86-87	37.5726355422572	41.0	37.0	41.0	27.0	41.0
88-89	37.50405539259198	41.0	37.0	41.0	27.0	41.0
90-91	37.33055123653101	41.0	37.0	41.0	27.0	41.0
92-93	37.22050361377437	41.0	37.0	41.0	27.0	41.0
94-95	37.05931608695396	41.0	37.0	41.0	27.0	41.0
96-97	37.00253654923492	41.0	37.0	41.0	27.0	41.0
98-99	36.878346856207344	41.0	37.0	41.0	27.0	41.0
100	36.75287015101726	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.412503421219915
1101	2	-0.48246910549284827
1101	3	0.031055095420486012
1101	4	0.12007011188420513
1101	5	-1.0759735205761345
1101	6	-0.6602808240604077
1101	7	-0.040075764171717765
1101	8	-0.17241778424374843
1101	9	-0.06468772709423831
1101	10-11	-0.05200316885736811
1101	12-13	0.042417455942178606
1101	14-15	0.08355639165802842
1101	16-17	-0.04497941182902565
1101	18-19	0.059692975140201554
1101	20-21	0.17901590345385188
1101	22-23	0.031446171110616206
1101	24-25	-0.017389013765871653
1101	26-27	0.07564988178203436
1101	28-29	0.013281915690690482
1101	30-31	-0.004944031286143513
1101	32-33	-0.014151394472918355
1101	34-35	0.01616952320929954
1101	36-37	0.05416017086696456
1101	38-39	0.13835750683414716
1101	40-41	0.1142867993109391
1101	42-43	0.24771062642416553
1101	44-45	0.05335636952332834
1101	46-47	0.023725122960172484
1101	48-49	0.06741369224383931
1101	50-51	0.13862271069643128
1101	52-53	0.09251272209436223
1101	54-55	-0.039995766471989214
1101	56-57	0.15868166803798545
1101	58-59	0.22948128437124637
1101	60-61	-0.021631888479419104
1101	62-63	-0.03719253820472801
1101	64-65	-0.032709616871450464
1101	66-67	-0.00773577160722283
1101	68-69	0.14373081579586966
1101	70-71	0.20927887777811804
1101	72-73	0.16459435516579646
1101	74-75	0.17553662295831174
1101	76-77	-0.20225227093498432
1101	78-79	-0.20990797328214228
1101	80-81	-0.05795551945703892
1101	82-83	-0.059856062237301444
1101	84-85	-0.005552573387099358
1101	86-87	-0.03132508734317696
1101	88-89	-0.1731644738362519
1101	90-91	-0.15144528745673114
1101	92-93	0.08949838076247829
1101	94-95	-9.091353057044671E-5
1101	96-97	-0.054275919341300494
1101	98-99	-0.21122505867571562
1101	100	-0.5767876617426353
1102	1	1.3517525609993086
1102	2	0.7790367458093268
1102	3	-0.3003997127180025
1102	4	-0.11849923788986239
1102	5	-0.7566020005752492
1102	6	-0.13902421754189476
1102	7	0.04092775087213596
1102	8	-0.12911929176542003
1102	9	-0.16662763762228394
1102	10-11	-0.1183403081044645
1102	12-13	-0.1634412450476006
1102	14-15	-0.18774110761161467
1102	16-17	-0.21895684180279318
1102	18-19	0.045678024334527834
1102	20-21	0.07208153250739002
1102	22-23	-0.05089718799323606
1102	24-25	-0.15844977173033925
1102	26-27	0.06893905209929585
1102	28-29	-0.15307291486950447
1102	30-31	-0.22204208759823274
1102	32-33	-0.10257477791834901
1102	34-35	-0.161549680130598
1102	36-37	-0.199416991848409
1102	38-39	-0.1992352859224269
1102	40-41	-0.15423908978583967
1102	42-43	-0.1437532543602984
1102	44-45	-0.06057204806235461
1102	46-47	-0.43554621673140304
1102	48-49	-0.24491751686124275
1102	50-51	-0.25640490403979044
1102	52-53	-0.16824380419812002
1102	54-55	-0.3011035199108747
1102	56-57	-0.16866956985143844
1102	58-59	-0.1335174295863908
1102	60-61	-0.041939644552080324
1102	62-63	-0.2971688100126073
1102	64-65	-0.26310596119812146
1102	66-67	-0.16445273520262305
1102	68-69	-0.32868286497798493
1102	70-71	-0.3372115018556343
1102	72-73	-0.22000257572926785
1102	74-75	-0.38252216002489803
1102	76-77	-0.19244979331943313
1102	78-79	-0.2703173097210154
1102	80-81	-0.2742136282643344
1102	82-83	-0.2943486992895288
1102	84-85	-0.3069733885598396
1102	86-87	-0.29324567084807995
1102	88-89	-0.29635778530698076
1102	90-91	-0.28126625691297136
1102	92-93	-0.4222188652701462
1102	94-95	-0.316652876271732
1102	96-97	-0.37552997872658267
1102	98-99	-0.35854188774089835
1102	100	-0.557689014218397
1103	1	1.5607115624853645
1103	2	0.7223822158694553
1103	3	0.33100054255098854
1103	4	1.0272101821144037
1103	5	2.9389499553420393
1103	6	0.06018285767568088
1103	7	-0.4547091741850764
1103	8	-0.34435624719458247
1103	9	-0.4443921176292278
1103	10-11	-0.3051386347547549
1103	12-13	-0.4984572083987757
1103	14-15	-0.3155256547984209
1103	16-17	-0.4301481814587973
1103	18-19	-0.4490858880062447
1103	20-21	-0.13035147746111875
1103	22-23	-0.09172449815952177
1103	24-25	-0.2675863044832383
1103	26-27	-0.14081034317351993
1103	28-29	-0.23301025464843406
1103	30-31	-0.15166467998861322
1103	32-33	-0.33710243989303734
1103	34-35	-0.2381030787154117
1103	36-37	-0.1909697938413899
1103	38-39	-0.13742349075952376
1103	40-41	-0.2042248077389175
1103	42-43	0.0498350104975529
1103	44-45	-0.4381696631413021
1103	46-47	-0.45213381991467116
1103	48-49	-0.2667245754465597
1103	50-51	-0.1768945793853618
1103	52-53	-0.2229125872592519
1103	54-55	-0.19770113860553806
1103	56-57	-0.1858693124130255
1103	58-59	-0.37848237529652806
1103	60-61	-0.27782699355106644
1103	62-63	-0.2632263503910224
1103	64-65	-0.2190890541471262
1103	66-67	-0.24816686285880962
1103	68-69	-0.2216183137415939
1103	70-71	-0.5586164937022104
1103	72-73	-0.4080947835167805
1103	74-75	-0.2382385945028389
1103	76-77	-0.34752462039818965
1103	78-79	-0.46238209597843394
1103	80-81	-0.34174493125705396
1103	82-83	-0.387167541467484
1103	84-85	-0.39395611632232885
1103	86-87	-0.2591486397059839
1103	88-89	-0.16233361068274377
1103	90-91	-0.5335226395416655
1103	92-93	-0.23766065056216235
1103	94-95	-0.3573142254370367
1103	96-97	-0.08853441520040661
1103	98-99	-0.29139782708377737
1103	100	-0.3899541468606529
1104	1	1.4090363001057344
1104	2	-0.5550729722143615
1104	3	-0.12743347483278988
1104	4	-0.019540277726184385
1104	5	-1.091913462986664
1104	6	-0.5044454829309473
1104	7	-0.5367555549691545
1104	8	-0.2125893773565224
1104	9	-0.17798031685741478
1104	10-11	-0.2618712419607334
1104	12-13	-0.12200194818311161
1104	14-15	-0.2564154012549409
1104	16-17	-0.24866983904886553
1104	18-19	-0.27130249997291855
1104	20-21	-0.242891600806594
1104	22-23	-0.09536084540228273
1104	24-25	-0.1831658839545085
1104	26-27	-0.2564782841696669
1104	28-29	-0.21036355648418947
1104	30-31	-0.19794808605490744
1104	32-33	-0.3381582699503838
1104	34-35	-0.23808504575115563
1104	36-37	-0.2994083194662309
1104	38-39	-0.2301093204562079
1104	40-41	-0.1970503929086007
1104	42-43	-0.260907226311339
1104	44-45	-0.2859939860034899
1104	46-47	-0.5405223942285176
1104	48-49	-0.48744174643513105
1104	50-51	-0.4015591662501521
1104	52-53	-0.3544711763601285
1104	54-55	-0.5783148003792604
1104	56-57	-0.4080751046011457
1104	58-59	-0.29739624985923285
1104	60-61	-0.28057882389694555
1104	62-63	-0.3196145279796596
1104	64-65	-0.3755520685374307
1104	66-67	-0.7223373465029113
1104	68-69	-0.7625423228302068
1104	70-71	-0.5634601479020915
1104	72-73	-0.43521906670645194
1104	74-75	-0.617721589417549
1104	76-77	-0.78381776426518
1104	78-79	-0.5723029312653338
1104	80-81	-0.5833037006875728
1104	82-83	-0.5771637545449906
1104	84-85	-0.22630094266488499
1104	86-87	-0.3780210384508891
1104	88-89	-0.28533465659467083
1104	90-91	-0.6255123968179603
1104	92-93	-0.5898002617714582
1104	94-95	-0.41243739058292306
1104	96-97	-0.39773393191696726
1104	98-99	-0.43803648254596794
1104	100	-0.5468499523931669
1105	1	1.4317993086548793
1105	2	0.2986214175613746
1105	3	-0.1167288212241342
1105	4	-0.0748155476854695
1105	5	-1.0724453287689144
1105	6	0.4553567031231509
1105	7	0.2750834309349983
1105	8	0.16958897105843107
1105	9	0.09036598076944102
1105	10-11	0.04809434278617175
1105	12-13	0.09818616423658
1105	14-15	0.023644925230051683
1105	16-17	0.07161207793105717
1105	18-19	0.20061114776623157
1105	20-21	0.1428584365069696
1105	22-23	0.14704410914458776
1105	24-25	0.08984677835947963
1105	26-27	-0.025249522528703494
1105	28-29	0.030251388378509603
1105	30-31	-0.03154539114794375
1105	32-33	-0.04924760035416398
1105	34-35	0.060173712522910705
1105	36-37	0.12114407745288247
1105	38-39	0.0770920235262409
1105	40-41	0.06479076238870363
1105	42-43	0.18401465817975549
1105	44-45	0.015553659076843473
1105	46-47	-0.25680707414757364
1105	48-49	0.03841854811640388
1105	50-51	-0.05328331315799062
1105	52-53	0.043556151051625136
1105	54-55	0.14982214213009115
1105	56-57	0.14852696621479566
1105	58-59	0.0688793186085519
1105	60-61	0.12251178037450217
1105	62-63	0.007954554176123452
1105	64-65	-0.010883846849743861
1105	66-67	0.048236744347804006
1105	68-69	0.11008080324797476
1105	70-71	0.16449760273224
1105	72-73	0.025411393656135317
1105	74-75	-0.051488714607152986
1105	76-77	-0.11578871869404139
1105	78-79	0.09292022119267074
1105	80-81	-0.05951691211836163
1105	82-83	0.06881445086356308
1105	84-85	0.06969739471220038
1105	86-87	-0.006103485804054287
1105	88-89	-0.006563817403971939
1105	90-91	0.028772762103102423
1105	92-93	0.0968052974286735
1105	94-95	0.07265210990128423
1105	96-97	0.06390729210672674
1105	98-99	0.08815607626194577
1105	100	-0.0584836269286555
1106	1	1.4310143220837563
1106	2	-0.5482856418976194
1106	3	-0.21575691609740666
1106	4	-0.12674189963368931
1106	5	-1.1417900569809056
1106	6	-0.5220660976103844
1106	7	-0.11329666092202473
1106	8	-0.0683453860537071
1106	9	-0.12515666991612306
1106	10-11	-0.09128741402396656
1106	12-13	-0.09271212036387055
1106	14-15	-0.11080806741231441
1106	16-17	-0.08611474708201428
1106	18-19	-0.01681874843034592
1106	20-21	-0.22225929193899674
1106	22-23	-0.07406596381328256
1106	24-25	-0.13709283935205718
1106	26-27	-0.12879273442529637
1106	28-29	-0.15372754543641776
1106	30-31	-0.08577496505824911
1106	32-33	0.028423677513913503
1106	34-35	-0.028050962187656125
1106	36-37	-0.23049634908367267
1106	38-39	0.005490373967013795
1106	40-41	-0.10907807111275503
1106	42-43	-0.05216596757007608
1106	44-45	0.011604004241554833
1106	46-47	-0.20786681451411226
1106	48-49	-0.08170189804821604
1106	50-51	0.10550876581777402
1106	52-53	0.14927948474346664
1106	54-55	0.03178539354446741
1106	56-57	-0.14695387289167883
1106	58-59	-0.24624886782950028
1106	60-61	-0.09444143187719334
1106	62-63	-0.22600372701592164
1106	64-65	-0.3868848533996285
1106	66-67	-0.4180607407557204
1106	68-69	-0.3971988427808455
1106	70-71	-0.6023212867632282
1106	72-73	-0.2726742585734101
1106	74-75	-0.3828755531009236
1106	76-77	-0.19443655723691222
1106	78-79	-0.2321210543187533
1106	80-81	-0.1941134791444057
1106	82-83	-0.167630640600116
1106	84-85	-0.42760111308270154
1106	86-87	-0.44843738680841483
1106	88-89	-0.4094869748646346
1106	90-91	-0.5560113096698203
1106	92-93	-0.49503473318240054
1106	94-95	-0.365989720605846
1106	96-97	-0.36834420399782175
1106	98-99	-0.42903665884026054
1106	100	-0.6117526967076756
1107	1	1.3336234972550454
1107	2	-0.6061875749836823
1107	3	-0.2672533426797621
1107	4	-0.274906364060552
1107	5	-1.2523524298922908
1107	6	-0.2870109632132092
1107	7	-0.3149082183717411
1107	8	-0.38318813438854704
1107	9	-0.2181319701731681
1107	10-11	-0.13957567932833115
1107	12-13	-0.2342702945867785
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2222	30-31	0.14287473021970953
2222	32-33	0.24122838067069807
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2222	88-89	0.35205772998052254
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2222	92-93	0.43512053886213664
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2223	6	0.3605609113036117
2223	7	0.36506465904056284
2223	8	0.31228368080186897
2223	9	0.4119462780155416
2223	10-11	0.32715956007857017
2223	12-13	0.30719424681876006
2223	14-15	0.2765761062415706
2223	16-17	0.4237941526677673
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2223	42-43	0.47309450167484357
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2223	66-67	0.5689598945477314
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2223	70-71	0.49276763084711206
2223	72-73	0.5023846038927573
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2223	82-83	0.40545498450509854
2223	84-85	0.3761853517304772
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2223	92-93	0.5226358387397383
2223	94-95	0.8470238480128813
2223	96-97	0.6849149783203288
2223	98-99	0.6908135152254857
2223	100	0.8305447998773872
2224	1	1.2238541587895515
2224	2	-0.8403934573766421
2224	3	-0.1603038356910691
2224	4	-0.31261863376297505
2224	5	-0.9334087408043956
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2224	7	0.07536802306842105
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2224	9	0.18222117205110777
2224	10-11	0.30143082703312274
2224	12-13	0.11481480785916176
2224	14-15	0.12809796399335482
2224	16-17	0.16501798326832073
2224	18-19	-0.00329588412364501
2224	20-21	0.10665878169359644
2224	22-23	0.08385692972733239
2224	24-25	0.14731308024730794
2224	26-27	0.051521174782621415
2224	28-29	0.09392938318556077
2224	30-31	0.03308545205472058
2224	32-33	0.21112415695681364
2224	34-35	0.14425488288533472
2224	36-37	0.0022708118439922487
2224	38-39	0.08136671249976501
2224	40-41	0.12474270085701988
2224	42-43	0.28757544304002636
2224	44-45	0.2223444838587767
2224	46-47	0.2947265219638737
2224	48-49	0.04606229087661973
2224	50-51	0.20445005895582113
2224	52-53	0.2468138119628307
2224	54-55	0.1636460181779995
2224	56-57	0.1631822491961188
2224	58-59	0.27849510912935216
2224	60-61	0.04663005476454174
2224	62-63	0.23395964007386993
2224	64-65	0.3533203606298301
2224	66-67	0.27827818088163525
2224	68-69	0.28583487646026384
2224	70-71	0.2856666421782421
2224	72-73	0.4242367694177247
2224	74-75	0.24224207820108745
2224	76-77	0.26072450251928814
2224	78-79	0.15578549236817452
2224	80-81	0.21439342217135504
2224	82-83	0.30535338433990233
2224	84-85	0.17720439590085846
2224	86-87	0.35771670407888223
2224	88-89	0.40153706030724834
2224	90-91	0.26047070057747845
2224	92-93	0.3022786969513973
2224	94-95	0.4245606849308956
2224	96-97	0.2865433242177673
2224	98-99	0.36878962197897636
2224	100	0.4361820379090702
2225	1	1.4029437774173452
2225	2	1.5260823785428332
2225	3	0.8472810131655919
2225	4	1.0823860497203395
2225	5	3.2969014264942196
2225	6	-0.03927589000197784
2225	7	0.4564559279104117
2225	8	0.370219217317576
2225	9	0.22957173701186662
2225	10-11	0.22924034343229494
2225	12-13	0.2604786205437648
2225	14-15	0.30737985981154736
2225	16-17	0.24937554801660866
2225	18-19	0.34413777650487276
2225	20-21	0.3593808381567527
2225	22-23	0.30777207239151494
2225	24-25	0.30972820146423174
2225	26-27	0.17216351526736418
2225	28-29	0.3587873474514325
2225	30-31	0.4447923148790309
2225	32-33	0.3318036560685016
2225	34-35	0.35437095745796654
2225	36-37	0.4352960028991859
2225	38-39	0.24204884513561353
2225	40-41	0.31039443324706895
2225	42-43	0.48982236785191446
2225	44-45	0.4242420178227633
2225	46-47	0.41615143809510613
2225	48-49	0.3499083833352685
2225	50-51	0.351682264961255
2225	52-53	0.4499373312289521
2225	54-55	0.39407264909257833
2225	56-57	0.5320676599710268
2225	58-59	0.5853094027812631
2225	60-61	0.46492721205476784
2225	62-63	0.4552178162187275
2225	64-65	0.44069700147000646
2225	66-67	0.5069443906717623
2225	68-69	0.6422756386260957
2225	70-71	0.6269985569997019
2225	72-73	0.6398795039335496
2225	74-75	0.5167266207110544
2225	76-77	0.5630857462210273
2225	78-79	0.5647578293914393
2225	80-81	0.3708736373222976
2225	82-83	0.4178988849538925
2225	84-85	0.36333345454564636
2225	86-87	0.4331290152058216
2225	88-89	0.529740308519898
2225	90-91	0.5423059177783784
2225	92-93	0.49815603457817303
2225	94-95	0.673421236833768
2225	96-97	0.6957268918250179
2225	98-99	0.7295732051479575
2225	100	0.7289536126068867
2226	1	1.7176275498734555
2226	2	1.5698969174614117
2226	3	0.9119164174582792
2226	4	0.7998407916548853
2226	5	2.9768946499390943
2226	6	-0.44326784234154104
2226	7	0.08035812096805017
2226	8	-0.006733177382670874
2226	9	0.010323112622486974
2226	10-11	0.045797943649631634
2226	12-13	-0.030112293058152773
2226	14-15	-0.06900289325419351
2226	16-17	0.0026680917008263805
2226	18-19	-0.11499137601473564
2226	20-21	-0.03925152069592741
2226	22-23	0.08580781370755375
2226	24-25	-0.013899922041488821
2226	26-27	-0.07182955981505756
2226	28-29	-0.053626930629917524
2226	30-31	-0.0431331871223577
2226	32-33	0.03919205592935526
2226	34-35	0.07258330021131343
2226	36-37	0.06835556741530269
2226	38-39	-0.03991599496875864
2226	40-41	-0.022010131103634478
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2226	44-45	0.07140403462982192
2226	46-47	0.005189207365440041
2226	48-49	0.07243702079657766
2226	50-51	0.07339541017501006
2226	52-53	0.17184369848416026
2226	54-55	0.24702140289812746
2226	56-57	0.3394937017911914
2226	58-59	0.35835069265241515
2226	60-61	0.2419557427552732
2226	62-63	0.2711075348012315
2226	64-65	0.19920102680389817
2226	66-67	0.3162976406614888
2226	68-69	0.24695086019238488
2226	70-71	0.4318247473828052
2226	72-73	0.3673822932478501
2226	74-75	0.3851767443631431
2226	76-77	0.46643688304829567
2226	78-79	0.23607410505287874
2226	80-81	0.19765797145056752
2226	82-83	0.2516662786968098
2226	84-85	0.14620901955095889
2226	86-87	0.1946709621822862
2226	88-89	0.2368788479717736
2226	90-91	0.3018762982177847
2226	92-93	0.07884766422940714
2226	94-95	0.4951294199250569
2226	96-97	0.32652518204803727
2226	98-99	0.3958667101807549
2226	100	0.43754670553290964
2227	1	1.2582459140212308
2227	2	1.4529484181599717
2227	3	0.7324125614838355
2227	4	0.03985620814088975
2227	5	3.1609660104810438
2227	6	0.2932362471037244
2227	7	0.2890069178521415
2227	8	0.30269533792819203
2227	9	0.2857853292611736
2227	10-11	0.2986755641743102
2227	12-13	0.219710750882534
2227	14-15	0.23945931226683115
2227	16-17	0.22322297402040903
2227	18-19	0.2143618356914132
2227	20-21	0.17037748305072853
2227	22-23	0.1601997704524507
2227	24-25	0.3374032527910984
2227	26-27	0.3189653898034166
2227	28-29	0.23746949281945007
2227	30-31	0.263052677887039
2227	32-33	0.2112702427134252
2227	34-35	0.1914060513870055
2227	36-37	0.2468792165271836
2227	38-39	0.11449901238741944
2227	40-41	0.3115307318489897
2227	42-43	0.3988829834788774
2227	44-45	0.2983172909548415
2227	46-47	0.3859217498626819
2227	48-49	0.3506304754606262
2227	50-51	0.29884484150679214
2227	52-53	0.4148080738014741
2227	54-55	0.33310172427256646
2227	56-57	0.540211902509391
2227	58-59	0.45469724488131646
2227	60-61	0.37984898358968877
2227	62-63	0.3885581816636403
2227	64-65	0.35396253450658577
2227	66-67	0.32916262411470143
2227	68-69	0.3938336541340064
2227	70-71	0.5365104697155871
2227	72-73	0.4179880203241666
2227	74-75	0.41453292077813586
2227	76-77	0.50733226217897
2227	78-79	0.44558359397413483
2227	80-81	0.42003707618262354
2227	82-83	0.2932907909095519
2227	84-85	0.2326210177276593
2227	86-87	0.2453100422331289
2227	88-89	0.37290710164708685
2227	90-91	0.3304551650319496
2227	92-93	0.37230381062673246
2227	94-95	0.5657256228742042
2227	96-97	0.4942637762572133
2227	98-99	0.6120986764127281
2227	100	0.672492469890436
2228	1	1.2808562609667913
2228	2	1.903831137374679
2228	3	-0.19166738757596846
2228	4	-0.2778860818012401
2228	5	3.1496256183993125
2228	6	0.35628319210070813
2228	7	0.5244278224914183
2228	8	0.43499897305213864
2228	9	0.38359445003564474
2228	10-11	0.2606422839975977
2228	12-13	0.24842800461992454
2228	14-15	0.13195803030946252
2228	16-17	0.26013066745510827
2228	18-19	0.08296824942272707
2228	20-21	0.19518828868345395
2228	22-23	0.22858585656617691
2228	24-25	0.2265871953594072
2228	26-27	0.11673168474941065
2228	28-29	0.06315146596314491
2228	30-31	0.29636525572016126
2228	32-33	0.20548452963520702
2228	34-35	0.22995915823181434
2228	36-37	0.2519816885917905
2228	38-39	0.2275803307467399
2228	40-41	0.17693084342153753
2228	42-43	0.33917335386593805
2228	44-45	0.22183092478273636
2228	46-47	0.3659527985883315
2228	48-49	0.39600190499805166
2228	50-51	0.39290670228903934
2228	52-53	0.4272515127155003
2228	54-55	0.2948951685398171
2228	56-57	0.5585129004639029
2228	58-59	0.5673009989032849
2228	60-61	0.5060851567638096
2228	62-63	0.3428092086382719
2228	64-65	0.2843592932675634
2228	66-67	0.34792569021862363
2228	68-69	0.3231949857918721
2228	70-71	0.4166192811297975
2228	72-73	0.5941092399586765
2228	74-75	0.43861258808906456
2228	76-77	0.5328911110657728
2228	78-79	0.5771589082649982
2228	80-81	0.5016047832539527
2228	82-83	0.3515832236983627
2228	84-85	0.4549152424392915
2228	86-87	0.35470890284069156
2228	88-89	0.4537352179304861
2228	90-91	0.4201823194247041
2228	92-93	0.4485760476564167
2228	94-95	0.7156160225631538
2228	96-97	0.327847396504815
2228	98-99	0.5844281197333032
2228	100	0.7252865734500489
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	20.0
13	27.0
14	142.0
15	477.0
16	1762.0
17	4540.0
18	7380.0
19	9492.0
20	11386.0
21	12919.0
22	14895.0
23	17014.0
24	19482.0
25	21956.0
26	24918.0
27	27585.0
28	31222.0
29	35449.0
30	40448.0
31	46915.0
32	55374.0
33	66009.0
34	80453.0
35	104445.0
36	147018.0
37	242446.0
38	594705.0
39	1102115.0
40	1213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.11547670194171	20.398359530330833	18.80690177192765	34.67926199579981
2	18.887742527154444	51.44041086780318	11.893392767780181	17.778453837262195
3	40.064067707935315	16.612560474508122	23.585241868640257	19.73812994891631
4	19.129674098981265	16.085337393379696	21.757706642055574	43.02728186558347
5	16.639785025247924	34.93302980959715	26.642290712643945	21.78489445251098
6	17.410559451658603	28.317353758971976	29.780021221188267	24.49206556818115
7	19.976500914098274	26.230064721684997	36.565951749719304	17.227482614497422
8	21.295403643460524	27.923461169928228	33.86194029850746	16.91919488810379
9	20.634666368825428	27.932425799321624	27.69170345593811	23.741204375914833
10-11	19.593740631227476	30.298628705625084	28.38231058178975	21.725320081357687
12-13	24.491955347327952	26.45361098211189	27.247127644565673	21.807306025994485
14-15	21.725062899366893	27.55475404584012	28.521666480515893	22.198516574277097
16-17	20.84590463397859	29.98227648680583	28.204946858852647	20.966872020362935
18-19	21.448849441253756	29.662636012532843	29.605688571714094	19.282825974499303
20-21	22.78384441518285	28.987202624138074	29.816651284734263	18.412301675944814
22-23	21.971847389676274	30.44303639345611	28.661738814787817	18.923377402079794
24-25	22.226053417434294	28.331443658039067	26.60025982729127	22.842243097235368
26-27	20.055033272001552	26.621422231105207	30.043081657486493	23.28046283940675
28-29	19.472649870655307	28.117673728055397	30.684507331002276	21.72516907028702
30-31	20.012849370979747	30.25620844714782	28.220334561317255	21.510607620555174
32-33	20.552588573477628	30.12839259786142	28.74640680018576	20.57261202847519
34-35	21.385472450665148	29.159551298254687	30.06918562955212	19.38579062152804
36-37	21.686540711174338	28.127186046921754	28.88809570697125	21.298177534932663
38-39	21.560109391507442	29.192788478583985	28.989851690995994	20.257250438912575
40-41	20.835771245937604	29.891671257749806	29.844257110698347	19.428300385614243
42-43	20.51329201391651	30.023015989214503	29.36121215216308	20.102479844705908
44-45	20.562820742682806	30.132874912558123	28.82374509884606	20.48055924591301
46-47	19.603821434902695	28.180280903572243	28.952706023049483	23.26319163847558
48-49	20.74251747367926	27.977487758137237	30.55682105424042	20.723173713943087
50-51	21.807452987132084	30.88250530529707	28.088829190008997	19.221212517561856
52-53	20.33921314791719	30.441832050803715	28.26953200849668	20.949422792782414
54-55	22.497580625750228	28.37549790786369	28.11108010016678	21.015841366219306
56-57	21.423840566836237	28.73650349449925	29.476373525326093	20.36328241333842
58-59	21.193699627723316	28.03565713210623	28.905570480674577	21.865072759495884
60-61	19.542652425616403	30.50551417571367	29.58702374859606	20.364809650073866
62-63	20.26717080412129	31.287424489615944	27.437717712421183	21.007686993841588
64-65	19.705761488100453	30.296892999885554	28.80580555811207	21.191539953901923
66-67	20.13444739673041	30.626921516874077	29.275485265676345	19.96314582071917
68-69	20.79233729932457	29.979814887751083	29.388296308924065	19.839551504000283
70-71	19.66479634125552	30.863492208120487	29.336014884224014	20.13569656639998
72-73	19.973873983763735	30.804266869668982	28.68424223898232	20.537616907584958
74-75	19.799357632867565	29.374206409856978	30.420661560257006	20.405774397018455
76-77	19.392899866559286	30.692631515522034	28.545566035517567	21.36890258240111
78-79	19.301122636130103	29.553370406729645	28.62101220953131	22.52449474760894
80-81	20.976603179915948	29.529045376747714	28.188903562585292	21.30544788075104
82-83	20.20107590248835	28.128453586733528	30.04006895416576	21.63040155661237
84-85	20.612346646052917	28.7033288167277	28.90451861409769	21.77980592312169
86-87	21.02269888250751	27.962222169969376	30.138734987919797	20.87634395960332
88-89	20.368317677073474	27.329774914321657	30.041703896821524	22.260203511783345
90-91	20.473670442588162	27.214024648322	30.33318294310253	21.979121965987314
92-93	20.43309079846925	27.169128020786182	30.759811125545962	21.6379700551986
94-95	20.79176782491638	27.24670513129508	30.659455038709567	21.302072005078976
96-97	20.516436133628822	27.55536026053271	30.713169334501185	21.215034271337288
98-99	21.563166836161844	28.309564818679352	29.593380576440364	20.53388776871844
100	20.658841475960095	29.75301711215486	28.63930887116211	20.948832540722933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	8.5
2	11.5
3	14.0
4	20.0
5	25.0
6	32.0
7	40.5
8	54.5
9	69.5
10	79.5
11	95.0
12	111.0
13	125.0
14	148.5
15	175.5
16	251.5
17	355.5
18	470.0
19	742.5
20	1178.0
21	1843.0
22	2887.5
23	4305.0
24	6543.0
25	10174.0
26	14778.5
27	19752.5
28	25164.5
29	31799.5
30	39549.0
31	48064.5
32	57291.0
33	67297.0
34	79499.5
35	94145.5
36	108199.0
37	117791.5
38	124740.5
39	130540.5
40	133263.0
41	133326.5
42	131705.5
43	129155.0
44	127243.0
45	123700.0
46	114940.5
47	105361.5
48	98963.0
49	94930.5
50	90905.5
51	83850.0
52	73527.0
53	62397.0
54	51680.5
55	41466.5
56	31852.0
57	23292.5
58	16676.5
59	12136.5
60	9436.5
61	7742.0
62	6311.5
63	4790.0
64	3524.5
65	2978.0
66	3122.5
67	2971.0
68	2259.5
69	1990.5
70	2219.0
71	2091.0
72	1337.5
73	647.0
74	342.0
75	227.0
76	161.0
77	127.0
78	106.5
79	80.5
80	57.5
81	37.5
82	28.0
83	23.0
84	24.5
85	24.5
86	19.5
87	14.0
88	12.5
89	12.5
90	11.0
91	11.5
92	10.5
93	10.0
94	14.5
95	13.0
96	13.5
97	20.0
98	39.0
99	79.0
100	86.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.320012285951103
2	0.0056580037974757955
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	4.041431283911282E-4
30-31	0.010066837925379012
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.001369677802402E-4
40-41	0.0017084232245624968
42-43	1.6533127979637065E-4
44-45	0.0
46-47	0.002039085784155238
48-49	0.0
50-51	0.0
52-53	0.0014145009493689489
54-55	0.020078565424159235
56-57	0.011628300012344736
58-59	0.016349426557641097
60-61	1.837014219959674E-4
62-63	0.01205081328293546
64-65	9.18507109979837E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.3881184859475757E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2721808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.84949921522347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8214420501233	63.37870517329927
2	8.794265254439399	12.989041700123789
3	2.4212583438999586	5.364261485030799
4	1.0496006514990002	3.1004992995669385
5	0.5535577906783582	2.0439982814141127
6	0.34210062927492013	1.5158376091899401
7	0.22464749324132982	1.1613073413079256
8	0.15417101341913853	0.9108361707605501
9	0.11849356733611345	0.7875621547197607
>10	0.4879707741825656	6.134414075577232
>50	0.022497548146577773	1.109939402513738
>100	0.009540229446103903	1.2686399664024208
>500	4.041371673160657E-4	0.19630764109589524
>1k	5.051714589900701E-5	0.038649698997753126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.6740284399193476E-5	0.0
3	0.0	0.0	0.0	3.6740284399193476E-5	0.0
4	0.0	0.0	0.0	3.6740284399193476E-5	0.0
5	0.0	0.0	0.0	3.6740284399193476E-5	0.0
6	0.0	0.0	0.0	3.6740284399193476E-5	0.0
7	0.0	0.0	0.0	3.6740284399193476E-5	0.0
8	0.0	0.0	0.0	3.6740284399193476E-5	0.0
9	0.0	0.0	0.0	3.6740284399193476E-5	0.0
10-11	0.0	0.0	0.0	3.6740284399193476E-5	0.0
12-13	0.0	0.0	0.0	3.6740284399193476E-5	0.0
14-15	0.0	0.0	0.0	3.6740284399193476E-5	0.0
16-17	0.0	0.0	0.0	3.6740284399193476E-5	0.0
18-19	0.0	0.0	0.0	3.6740284399193476E-5	0.0
20-21	0.0	0.0	0.0	3.6740284399193476E-5	0.0
22-23	0.0	0.0	0.0	3.6740284399193476E-5	0.0
24-25	0.0	0.0	0.0	3.6740284399193476E-5	0.0
26-27	0.0	0.0	0.0	3.6740284399193476E-5	0.0
28-29	0.0	0.0	0.0	3.6740284399193476E-5	0.0
30-31	0.0	0.0	0.0	3.6740284399193476E-5	0.0
32-33	0.0	0.0	0.0	3.6740284399193476E-5	0.0
34-35	0.0	0.0	0.0	3.6740284399193476E-5	0.0
36-37	0.0	0.0	0.0	3.6740284399193476E-5	0.0
38-39	3.6740284399193476E-5	0.0	0.0	3.6740284399193476E-5	0.0
40-41	3.6740284399193476E-5	0.0	0.0	3.6740284399193476E-5	0.0
42-43	3.6740284399193476E-5	0.0	0.0	3.6740284399193476E-5	0.0
44-45	3.6740284399193476E-5	0.0	0.0	3.6740284399193476E-5	0.0
46-47	3.6740284399193476E-5	0.0	0.0	3.6740284399193476E-5	0.0
48-49	3.6740284399193476E-5	0.0	0.0	3.6740284399193476E-5	0.0
50-51	3.6740284399193476E-5	0.0	0.0	7.348056879838695E-5	0.0
52-53	3.6740284399193476E-5	0.0	0.0	7.348056879838695E-5	0.0
54-55	1.2859099539717716E-4	0.0	0.0	7.348056879838695E-5	0.0
56-57	2.2044170639516086E-4	0.0	0.0	7.348056879838695E-5	0.0
58-59	2.939222751935478E-4	0.0	0.0	7.348056879838695E-5	0.0
60-61	3.306625595927413E-4	0.0	0.0	7.348056879838695E-5	0.0
62-63	4.2251327059072497E-4	0.0	0.0	7.348056879838695E-5	0.0
64-65	4.776236971895152E-4	0.0	0.0	7.348056879838695E-5	0.0
66-67	5.143639815887086E-4	0.0	0.0	7.348056879838695E-5	0.0
68-69	5.143639815887086E-4	0.0	0.0	7.348056879838695E-5	0.0
70-71	5.878445503870956E-4	0.0	0.0	7.348056879838695E-5	0.0
72-73	5.878445503870956E-4	0.0	0.0	7.348056879838695E-5	0.0
74-75	7.71545972383063E-4	0.0	0.0	7.348056879838695E-5	0.0
76-77	8.450265411814499E-4	0.0	0.0	7.348056879838695E-5	0.0
78-79	9.552473943790304E-4	0.0	0.0	7.348056879838695E-5	0.0
80-81	0.0011022085319758045	0.0	0.0	7.348056879838695E-5	0.0
82-83	0.0014328710915685458	0.0	0.0	7.348056879838695E-5	0.0
84-85	0.0015247218025665294	0.0	0.0	7.348056879838695E-5	0.0
86-87	0.0015430919447661261	3.6740284399193476E-5	0.0	7.348056879838695E-5	0.0
88	0.0015430919447661261	3.6740284399193476E-5	0.0	7.348056879838695E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10550	0.0	80.61813	8
CACCGGC	10410	0.0	78.3657	9
CTTCACC	12025	0.0	74.632805	6
CGTAGTG	2575	0.0	74.00751	4
CCTTCAC	13340	0.0	71.67408	5
CGGGCCC	775	0.0	69.04466	7
CCGCGTA	300	0.0	68.82864	1
GTGTGGC	4270	0.0	68.703545	7
TTCACCG	13155	0.0	67.40127	7
GCGTAGT	1795	0.0	66.68109	3
CGGGGCA	1150	0.0	66.52988	94
CAGCGTA	1220	0.0	65.09644	1
AACGGGC	845	0.0	63.324986	5
GGCCCGA	825	0.0	62.584347	9
GCCTTCA	4185	0.0	62.247868	4
GGGCCCG	855	0.0	62.035355	8
GCAGCCT	60	9.98989E-9	61.769295	1
TGTGGCA	4665	0.0	60.77322	8
TAGTGTG	2880	0.0	58.835804	6
ACCTTCA	4975	0.0	58.59027	4
>>END_MODULE