FastQCFastQC Report
Tue 7 Mar 2017
1HL_25A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_25A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2957874
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA147290.4979590070435725No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG82190.2778684960887448No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA72330.24453374281663115No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT59730.2019355794060193No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG55960.18918993844903467No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA54670.1848286979093768No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG54470.18415253658539882No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG48040.16241395001950726No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46900.15855983047283284No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC46590.157511780420667No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA45080.15240676242463336No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG45040.15227153015983777No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA41170.13918780854086416No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA38070.12870730801920569No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC37900.1281325708938244No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT36850.1245827239429401No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35130.11876773655672959No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA33540.11339225403110477No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC31640.1069687214533141No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30940.10460215681939124No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG30820.10419646002500443No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC29710.10044376467692674No Hit
CAGCCTTATTCAGGACACATCAGTACAAGTGACAGCCCTTCAACCGCTGA29620.10013949208113665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCA550.086.03
GCTGCAG550.086.03
GCTGCAC500.086.03
AGCTGCG550.086.02
AGCTGCC2750.086.02
CAGCTGC6700.086.01
GCAGCCG450.086.03
GCAGCCA450.086.03
GCAGCAT450.086.03
AGCAGCC1250.086.02
GCTGCCT600.085.999993
CTGCAGC600.085.999991
GCAGCTC159.829784E-485.999993
AGCTGCA2000.083.8500062
GCTGCCC1950.083.7948763
CAGCAGC5550.083.6756741
AGCAGCG1050.081.9047552
CAGCGTA60900.080.775041
GCTGCAA800.080.6253
GCAGCAA800.080.6253
Produced by FastQC (version 0.11.5)