##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_25A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2957874 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.806354834587275 12.0 12.0 12.0 2.0 32.0 2 14.627581499414783 12.0 12.0 12.0 12.0 22.0 3 13.732668463903465 12.0 12.0 12.0 12.0 22.0 4 13.712517166045613 12.0 12.0 12.0 12.0 22.0 5 22.446438894963073 22.0 22.0 22.0 22.0 27.0 6 26.768736937408423 27.0 22.0 32.0 22.0 32.0 7 34.938120420274835 37.0 37.0 37.0 27.0 41.0 8 37.5882187679394 41.0 37.0 41.0 27.0 41.0 9 38.53282797035979 41.0 41.0 41.0 32.0 41.0 10-11 38.89270384742555 41.0 41.0 41.0 37.0 41.0 12-13 39.06973944799542 41.0 41.0 41.0 37.0 41.0 14-15 39.02238753239658 41.0 41.0 41.0 37.0 41.0 16-17 38.92981901866003 41.0 41.0 41.0 37.0 41.0 18-19 38.86510226601945 41.0 41.0 41.0 37.0 41.0 20-21 38.871021720330205 41.0 41.0 41.0 37.0 41.0 22-23 38.86551911947568 41.0 41.0 41.0 37.0 41.0 24-25 38.88587005396444 41.0 41.0 41.0 37.0 41.0 26-27 38.85362003249631 41.0 41.0 41.0 37.0 41.0 28-29 38.80589403064498 41.0 41.0 41.0 34.5 41.0 30-31 38.684129209019716 41.0 41.0 41.0 32.0 41.0 32-33 38.722307305855495 41.0 41.0 41.0 32.0 41.0 34-35 38.69472448792612 41.0 41.0 41.0 32.0 41.0 36-37 38.61935971579587 41.0 41.0 41.0 32.0 41.0 38-39 38.52498399188065 41.0 41.0 41.0 32.0 41.0 40-41 38.57091461637649 41.0 41.0 41.0 32.0 41.0 42-43 38.30192445655224 41.0 41.0 41.0 32.0 41.0 44-45 38.41163974530355 41.0 41.0 41.0 32.0 41.0 46-47 38.430493996701685 41.0 41.0 41.0 32.0 41.0 48-49 38.371803193780394 41.0 41.0 41.0 32.0 41.0 50-51 38.25235168908479 41.0 41.0 41.0 32.0 41.0 52-53 38.228953971670194 41.0 41.0 41.0 32.0 41.0 54-55 38.094233560996855 41.0 37.0 41.0 32.0 41.0 56-57 37.88799117203776 41.0 37.0 41.0 29.5 41.0 58-59 37.877515742726025 41.0 37.0 41.0 29.5 41.0 60-61 37.87953661988307 41.0 37.0 41.0 29.5 41.0 62-63 37.87950974247043 41.0 37.0 41.0 29.5 41.0 64-65 37.860436245763005 41.0 37.0 41.0 29.5 41.0 66-67 37.71909131355832 41.0 37.0 41.0 27.0 41.0 68-69 37.75223352989343 41.0 37.0 41.0 27.0 41.0 70-71 37.72211341659584 41.0 37.0 41.0 27.0 41.0 72-73 37.56823870793685 41.0 37.0 41.0 27.0 41.0 74-75 37.507341928696086 41.0 37.0 41.0 27.0 41.0 76-77 36.409759002580905 39.0 34.5 41.0 24.5 41.0 78-79 36.94842883773954 41.0 37.0 41.0 27.0 41.0 80-81 37.60926378202723 41.0 37.0 41.0 27.0 41.0 82-83 37.79896726500182 41.0 37.0 41.0 27.0 41.0 84-85 37.724716975773816 41.0 37.0 41.0 27.0 41.0 86-87 37.57798709478497 41.0 37.0 41.0 27.0 41.0 88-89 37.50770942237567 41.0 37.0 41.0 27.0 41.0 90-91 37.34402293674443 41.0 37.0 41.0 27.0 41.0 92-93 37.21973451201775 41.0 37.0 41.0 27.0 41.0 94-95 37.0604606551868 41.0 37.0 41.0 27.0 41.0 96-97 36.991632503615776 41.0 37.0 41.0 27.0 41.0 98-99 36.871788994392595 41.0 37.0 41.0 27.0 41.0 100 36.73514186202658 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4902425631577998 1101 2 -0.5404739776608505 1101 3 -0.01958097845794704 1101 4 -0.03403659498821021 1101 5 -1.0538078990496516 1101 6 -0.7945613201497181 1101 7 -0.17792210769468397 1101 8 -0.06751119180341192 1101 9 -0.21482327571746396 1101 10-11 -0.07272624887978196 1101 12-13 -0.016747235058325316 1101 14-15 0.03660733801489613 1101 16-17 -0.23174486949176298 1101 18-19 0.04723102362807907 1101 20-21 0.19170271008269424 1101 22-23 0.12500195682539328 1101 24-25 -0.036846428195318026 1101 26-27 0.09911472320232662 1101 28-29 0.019168932829913388 1101 30-31 0.06116391418154876 1101 32-33 -0.10068886074422068 1101 34-35 0.0567986928300499 1101 36-37 -0.033623638587130245 1101 38-39 0.18333849552244885 1101 40-41 -0.025329689677533906 1101 42-43 0.19328809495090127 1101 44-45 -0.03590356590310506 1101 46-47 0.035505399976280216 1101 48-49 -0.003613290810427827 1101 50-51 0.13442486800489917 1101 52-53 0.03648325336050817 1101 54-55 -0.0077394826788648174 1101 56-57 0.12542131692409697 1101 58-59 0.1387631080739382 1101 60-61 -0.03393337524248352 1101 62-63 0.033779058762043235 1101 64-65 0.13083953915018043 1101 66-67 0.010978196308947474 1101 68-69 -0.11384823682783463 1101 70-71 0.009454247631090595 1101 72-73 0.10992349160995474 1101 74-75 0.07702603543404507 1101 76-77 -0.2639162833687152 1101 78-79 0.033002434948741666 1101 80-81 -0.028974849609575415 1101 82-83 -0.024481882857109838 1101 84-85 -0.11572604873789771 1101 86-87 0.10267039620080709 1101 88-89 -0.26023956379862057 1101 90-91 -0.08330610064776778 1101 92-93 0.03284257869273688 1101 94-95 0.22100613473102015 1101 96-97 -0.015116466784512284 1101 98-99 -0.2874400273428961 1101 100 -0.5089228600833167 1102 1 1.567864675993846 1102 2 0.9419190552812164 1102 3 -0.15778620375139596 1102 4 -0.032143372723917096 1102 5 -0.7527855590269326 1102 6 -0.1538949059126864 1102 7 0.11174089874226922 1102 8 -0.053879991500494384 1102 9 0.0017613513177536788 1102 10-11 -0.06326013755831639 1102 12-13 -0.06729626951497636 1102 14-15 -0.054078008444783165 1102 16-17 0.08972427098532876 1102 18-19 0.041172712382334 1102 20-21 0.06996851848860075 1102 22-23 -0.0652668807361323 1102 24-25 -0.07811867355692215 1102 26-27 -0.026784557373211726 1102 28-29 -0.011690590078075047 1102 30-31 0.05302305844507771 1102 32-33 -0.04010574828682678 1102 34-35 -0.06455685431118496 1102 36-37 0.04418779647534876 1102 38-39 0.07447821532555565 1102 40-41 -0.006965433713887137 1102 42-43 0.14463228275855045 1102 44-45 0.0958647036917526 1102 46-47 -0.34010989725961593 1102 48-49 -0.21054248429774702 1102 50-51 -0.11093673744758092 1102 52-53 -0.10834824985796132 1102 54-55 -0.2315183543183963 1102 56-57 -0.07336702082675828 1102 58-59 -0.1562009207030357 1102 60-61 0.004120392269825857 1102 62-63 -0.24111681401342366 1102 64-65 -0.2256542132163517 1102 66-67 -0.17607216340328335 1102 68-69 -0.15549912664230447 1102 70-71 -0.22151886861275472 1102 72-73 -0.19942902637566107 1102 74-75 -0.29972519516042695 1102 76-77 -0.1732309369090359 1102 78-79 -0.16995099178355133 1102 80-81 -0.309361066951503 1102 82-83 -0.24769278781735693 1102 84-85 -0.1605954164016623 1102 86-87 -0.05389908505628682 1102 88-89 -0.18640389549116065 1102 90-91 -0.2438513486598879 1102 92-93 -0.24735431642275074 1102 94-95 -0.14362847337197593 1102 96-97 -0.13495743611430555 1102 98-99 -0.15699701333305427 1102 100 -0.4278929471715358 1103 1 1.4556390843731482 1103 2 0.7934572495765675 1103 3 0.3882958170629376 1103 4 0.9769110640972585 1103 5 2.8746042775073626 1103 6 -0.37163483392519936 1103 7 -0.446177889979829 1103 8 -0.26085827964922714 1103 9 -0.4394118339288653 1103 10-11 -0.2502585500141308 1103 12-13 -0.4537924901268653 1103 14-15 -0.4546722790527511 1103 16-17 -0.3021746894990258 1103 18-19 -0.26217734516501423 1103 20-21 -0.0611722552732914 1103 22-23 -0.2747096608331887 1103 24-25 -0.2816474987291784 1103 26-27 -0.2502406353673692 1103 28-29 -0.26311193307380165 1103 30-31 -0.39556845025038 1103 32-33 -0.4214913197411718 1103 34-35 -0.4410027821717577 1103 36-37 -0.4272541242399299 1103 38-39 -0.3197236901478533 1103 40-41 -0.42898074103895567 1103 42-43 -0.3641811848529386 1103 44-45 -0.5612607333182993 1103 46-47 -0.6005519714144754 1103 48-49 -0.49528146134516504 1103 50-51 -0.4165680452482903 1103 52-53 -0.4347341381005023 1103 54-55 -0.4556757953424935 1103 56-57 -0.44947454083771277 1103 58-59 -0.5521138577018192 1103 60-61 -0.274624982397512 1103 62-63 -0.15565802215046176 1103 64-65 -0.40241954163561644 1103 66-67 -0.47634457121266394 1103 68-69 -0.5117198556394698 1103 70-71 -0.7856769582268726 1103 72-73 -0.4798768747833506 1103 74-75 -0.37613495511906336 1103 76-77 -0.669562800004897 1103 78-79 -0.7964930821231349 1103 80-81 -0.22489490308222315 1103 82-83 -0.4682236047014712 1103 84-85 -0.4149420205074108 1103 86-87 -0.24728881169060912 1103 88-89 -0.22133181942356117 1103 90-91 -0.48650749295435247 1103 92-93 -0.2865933371599141 1103 94-95 -0.4181937453999396 1103 96-97 -0.2695089376152211 1103 98-99 -0.20420040425777586 1103 100 -0.6748613142951072 1104 1 1.530537595418716 1104 2 -0.5230088924570637 1104 3 0.10962668240078699 1104 4 -0.06990019864674224 1104 5 -1.0880418380676886 1104 6 -0.5492256311969115 1104 7 -0.3258640301367066 1104 8 -0.038158486399048286 1104 9 -0.07371335256793543 1104 10-11 -0.059492434905202174 1104 12-13 -0.019285294975517786 1104 14-15 -0.18563945851540353 1104 16-17 -0.03757984493551447 1104 18-19 0.031776004568463634 1104 20-21 0.05845683395418888 1104 22-23 -0.029080785040704882 1104 24-25 -0.0636320823438794 1104 26-27 0.03733220392528125 1104 28-29 0.14829123030271774 1104 30-31 0.07093421492714924 1104 32-33 -0.03833471426533919 1104 34-35 -0.06319631128876324 1104 36-37 -0.017749489695226828 1104 38-39 -0.018507110664472748 1104 40-41 0.12649319198538933 1104 42-43 0.022408166825997 1104 44-45 0.00764933142978208 1104 46-47 -0.12299193208846759 1104 48-49 -0.10448382164184267 1104 50-51 -0.02554194423343148 1104 52-53 -0.01602496597683256 1104 54-55 -0.029665061034116036 1104 56-57 0.07227052945784607 1104 58-59 0.06617267632848467 1104 60-61 0.08150127666964124 1104 62-63 -0.03908847454065523 1104 64-65 -0.038557950551314946 1104 66-67 -0.4744297894284841 1104 68-69 -0.28933346837638396 1104 70-71 -0.11483052286731521 1104 72-73 0.04740615903566692 1104 74-75 -0.07636442840687607 1104 76-77 -0.18719004682222362 1104 78-79 0.00522089903448375 1104 80-81 -0.10673379277864825 1104 82-83 -0.22942970869706159 1104 84-85 -0.11987979009943217 1104 86-87 -0.20402253251886293 1104 88-89 -0.20318739139561615 1104 90-91 -0.1170676126251422 1104 92-93 -0.19536735549692708 1104 94-95 -0.25976594561423383 1104 96-97 -0.21963491332902407 1104 98-99 -0.16498632343583353 1104 100 -0.1335217766272052 1105 1 1.6938376577779266 1105 2 0.41157738223070695 1105 3 -0.01248193424398103 1105 4 -0.29099855495428884 1105 5 -1.198083227782206 1105 6 0.33941999900268627 1105 7 -0.14416609133920133 1105 8 -0.19661902752895344 1105 9 -0.2845309573104089 1105 10-11 -0.1621660089632968 1105 12-13 -0.2653782980253325 1105 14-15 -0.3350588687082947 1105 16-17 -0.17516657285108295 1105 18-19 -0.2808339795435941 1105 20-21 -0.2810079254546878 1105 22-23 -0.1938887918106289 1105 24-25 -0.10167839734923945 1105 26-27 -0.19464736469466715 1105 28-29 -0.21726871458149333 1105 30-31 -0.14777764586524 1105 32-33 -0.15753046843434504 1105 34-35 -0.10920245791545113 1105 36-37 -0.17905122279956487 1105 38-39 -0.32443543605089786 1105 40-41 -0.1283548474592351 1105 42-43 -0.07907432574479856 1105 44-45 -0.09593862898342564 1105 46-47 -0.5111506287897711 1105 48-49 -0.2427481148399906 1105 50-51 -0.2963067096050622 1105 52-53 -0.47987755209231153 1105 54-55 -0.32994779234991967 1105 56-57 -0.14347060374090148 1105 58-59 -0.21898816666934806 1105 60-61 -0.41349272973781126 1105 62-63 -0.4405052593608545 1105 64-65 -0.49913660466741305 1105 66-67 -0.397385028556819 1105 68-69 -0.3975373468666916 1105 70-71 -0.39170035190674213 1105 72-73 -0.3563842705438063 1105 74-75 -0.48282509819120634 1105 76-77 -0.6006023146384436 1105 78-79 -0.48741840829949723 1105 80-81 -0.6085431057728314 1105 82-83 -0.5508359250556225 1105 84-85 -0.26697372750933823 1105 86-87 -0.26043676334310817 1105 88-89 -0.3615219216850747 1105 90-91 -0.486461562166177 1105 92-93 -0.4122992030185202 1105 94-95 -0.2827104194807859 1105 96-97 -0.2869638418638374 1105 98-99 -0.4499796660099662 1105 100 -0.7798250621028586 1106 1 1.277896147646553 1106 2 -0.6649705361954457 1106 3 -0.1784726434292807 1106 4 -0.30225370088485626 1106 5 -1.0847257027959856 1106 6 -0.6862394680208759 1106 7 -0.01994556048322238 1106 8 0.13562149375589883 1106 9 -0.013295955760000311 1106 10-11 -0.11358987678595156 1106 12-13 -0.10196641806957274 1106 14-15 -0.10495696312545277 1106 16-17 -0.07217330967209534 1106 18-19 0.08123322309623404 1106 20-21 0.09003805006269516 1106 22-23 0.10475229764159621 1106 24-25 -0.24216511578323718 1106 26-27 -0.04162930228775252 1106 28-29 -0.17337640495641438 1106 30-31 -0.108994848613996 1106 32-33 -0.052613467782954615 1106 34-35 -0.1102236196812143 1106 36-37 -0.19904818734502072 1106 38-39 -0.0063454340974615775 1106 40-41 -0.09903409940123709 1106 42-43 0.023145332236452987 1106 44-45 0.016109801766454268 1106 46-47 -0.21119528918558217 1106 48-49 -0.1587610816046663 1106 50-51 -0.09583432606884656 1106 52-53 -0.1179157915577278 1106 54-55 -0.24864466735672153 1106 56-57 -0.03709317183712102 1106 58-59 -0.04627211020408595 1106 60-61 -0.10256981358099893 1106 62-63 -0.1691155803420159 1106 64-65 -0.4570145169427775 1106 66-67 -0.3053568729004539 1106 68-69 -0.2729681744492467 1106 70-71 -0.46770835156397794 1106 72-73 -0.30119012127351397 1106 74-75 -0.4888099543230098 1106 76-77 -0.3609455533812991 1106 78-79 -0.12832500707267513 1106 80-81 -0.20675131616905418 1106 82-83 -0.31382915918720045 1106 84-85 -0.42510050348626294 1106 86-87 -0.19018763095299107 1106 88-89 -0.31801144028958106 1106 90-91 -0.4037753446547967 1106 92-93 -0.37143639321880073 1106 94-95 -0.3016720616471744 1106 96-97 -0.35618375183138795 1106 98-99 -0.4011177799179322 1106 100 -0.3140578001336891 1107 1 1.2200240544256324 1107 2 -0.45424637560724257 1107 3 -0.07276244809313503 1107 4 -0.2715397546276108 1107 5 -1.1998919147943496 1107 6 -0.32240573840796216 1107 7 -0.31294830031859533 1107 8 -0.16476719775283044 1107 9 -0.10365095180213757 1107 10-11 -0.02888801773337235 1107 12-13 -0.3105654508862514 1107 14-15 -0.22674039122343714 1107 16-17 -0.13358989399095123 1107 18-19 -0.13226927985830628 1107 20-21 -0.1583622098040678 1107 22-23 -0.1386008853937426 1107 24-25 -0.1439656318212883 1107 26-27 -0.1299962731917006 1107 28-29 0.0020072791650349586 1107 30-31 -0.1628276247908076 1107 32-33 -0.1400613706300362 1107 34-35 -0.32532227814488124 1107 36-37 -0.16834458845632838 1107 38-39 -0.08711536700955946 1107 40-41 -0.24872179364537317 1107 42-43 -0.06294662187148958 1107 44-45 -0.1200698462899723 1107 46-47 -0.23269364727992325 1107 48-49 -0.40821647742477296 1107 50-51 -0.272623700208257 1107 52-53 -0.17574191289836705 1107 54-55 -0.09907219845241144 1107 56-57 -0.09199224434334496 1107 58-59 -0.29135825574278584 1107 60-61 -0.19795096998811346 1107 62-63 -0.32603408964097014 1107 64-65 -0.4169121075580122 1107 66-67 -0.39933780861299084 1107 68-69 -0.6395288052248276 1107 70-71 -0.3864175764647726 1107 72-73 -0.26805149092512437 1107 74-75 -0.3260351300619746 1107 76-77 -0.4159115142342529 1107 78-79 -0.5685861007451933 1107 80-81 -0.3220350447102689 1107 82-83 -0.2696534342616488 1107 84-85 -0.09478951332438612 1107 86-87 -0.10211807298518494 1107 88-89 0.01877434183475657 1107 90-91 -0.1055543529482108 1107 92-93 -0.057761828307164365 1107 94-95 -0.020220532786517253 1107 96-97 -0.16523259954102798 1107 98-99 -0.44878574173023367 1107 100 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0.32853175822445735 2223 24-25 0.3155830597853182 2223 26-27 0.4240869305284747 2223 28-29 0.3327107662015578 2223 30-31 0.31926939224189255 2223 32-33 0.2816325190435762 2223 34-35 0.3445902095127096 2223 36-37 0.3266780770555897 2223 38-39 0.2934015454529302 2223 40-41 0.31585603891804226 2223 42-43 0.5373831297207801 2223 44-45 0.5525964876087599 2223 46-47 0.44995426993563825 2223 48-49 0.30417142252778007 2223 50-51 0.26285260142381617 2223 52-53 0.23356868863132974 2223 54-55 0.34180848823861965 2223 56-57 0.4154043256639781 2223 58-59 0.37885276287190806 2223 60-61 0.5030480175962282 2223 62-63 0.42014760194425804 2223 64-65 0.43643019528244764 2223 66-67 0.45563033862853075 2223 68-69 0.5686534016609102 2223 70-71 0.5267595246978019 2223 72-73 0.5497340582270809 2223 74-75 0.5880068670752152 2223 76-77 0.5074381165810138 2223 78-79 0.7223272977809785 2223 80-81 0.5504404446906435 2223 82-83 0.27765601184267297 2223 84-85 0.2519707056506846 2223 86-87 0.5880382178636907 2223 88-89 0.5033482783654932 2223 90-91 0.38339192733715066 2223 92-93 0.6331875626940473 2223 94-95 0.6258128244108647 2223 96-97 0.6474910895450918 2223 98-99 0.7588278451526804 2223 100 0.7445852301819897 2224 1 1.2557896057645195 2224 2 -0.9016484701464957 2224 3 -0.05683721983785439 2224 4 -0.32034054342509855 2224 5 -1.0727294012947155 2224 6 0.6702281047924181 2224 7 0.35100216898935344 2224 8 0.09214940897626178 2224 9 0.20591674905951862 2224 10-11 0.10431976406845678 2224 12-13 0.08825298721125563 2224 14-15 0.1497055344294509 2224 16-17 0.08744325619532844 2224 18-19 0.13416766135967606 2224 20-21 0.07265669398979924 2224 22-23 0.20980352011669368 2224 24-25 0.24729950414982227 2224 26-27 0.29974725526453483 2224 28-29 0.12865942948307918 2224 30-31 0.07235422282751358 2224 32-33 0.09491852054988215 2224 34-35 0.1932958728651215 2224 36-37 0.08317924145490707 2224 38-39 0.20276800168804243 2224 40-41 0.1618221564678919 2224 42-43 0.3069650499119376 2224 44-45 0.3499296998917245 2224 46-47 0.23552250973128253 2224 48-49 0.1436656792423534 2224 50-51 0.258100309245755 2224 52-53 0.3086908791654466 2224 54-55 0.23815499801720108 2224 56-57 0.43308172730949934 2224 58-59 0.46245472996865544 2224 60-61 0.2234768853789717 2224 62-63 0.3136169634874335 2224 64-65 0.36403213510023846 2224 66-67 0.18999683393506217 2224 68-69 0.30931273966604067 2224 70-71 0.4865173131588847 2224 72-73 0.5415159180919673 2224 74-75 0.3284748972065188 2224 76-77 0.5516723615286878 2224 78-79 0.30981182727514067 2224 80-81 0.28623965340206325 2224 82-83 0.2944649624085258 2224 84-85 0.04574436961062389 2224 86-87 0.26162137811063957 2224 88-89 0.24379080551622678 2224 90-91 0.29476423917026295 2224 92-93 0.1999087588534536 2224 94-95 0.27018910462142287 2224 96-97 0.2654702219976244 2224 98-99 0.2208524862887984 2224 100 0.49533742603124153 2225 1 1.4904127886579506 2225 2 2.0822825682606254 2225 3 0.8001134538950829 2225 4 1.1559655321153084 2225 5 3.2146687558104254 2225 6 0.09594506066017416 2225 7 0.30057819706618005 2225 8 0.349548006760628 2225 9 0.2714461780889863 2225 10-11 0.26241586151875396 2225 12-13 0.24250255398363407 2225 14-15 0.23256421059767263 2225 16-17 0.20437035858108743 2225 18-19 0.12282466360286293 2225 20-21 0.24468167779234307 2225 22-23 0.22798816984730053 2225 24-25 0.2931127448635067 2225 26-27 0.20710742553500694 2225 28-29 0.18035338474743412 2225 30-31 0.33491055026102856 2225 32-33 0.26420459025164433 2225 34-35 0.2224808886875067 2225 36-37 0.2053480735109119 2225 38-39 0.2514728254712537 2225 40-41 0.26493246760596634 2225 42-43 0.5182211097861256 2225 44-45 0.3036522485350801 2225 46-47 0.29495345165960174 2225 48-49 0.36127393765664806 2225 50-51 0.36367956497704057 2225 52-53 0.30435575074168497 2225 54-55 0.36949166938872224 2225 56-57 0.5549620499551509 2225 58-59 0.5065263607370767 2225 60-61 0.43189013638942697 2225 62-63 0.3063869005501729 2225 64-65 0.3777871742101624 2225 66-67 0.39373802944218284 2225 68-69 0.48483520482279374 2225 70-71 0.4978108949440312 2225 72-73 0.46432139614835677 2225 74-75 0.33531505172662435 2225 76-77 0.6154738716180361 2225 78-79 0.5750126579198351 2225 80-81 0.40477181221829284 2225 82-83 0.34269516468028627 2225 84-85 0.33947846718727703 2225 86-87 0.2722664911420054 2225 88-89 0.5112497963208682 2225 90-91 0.522476388069073 2225 92-93 0.5307812766278772 2225 94-95 0.719245373654239 2225 96-97 0.4717386914844397 2225 98-99 0.6525298069819172 2225 100 0.7335078627871425 2226 1 1.5130820743036413 2226 2 1.4865628475262564 2226 3 0.928520562705538 2226 4 0.8585572806391699 2226 5 3.1896282590787166 2226 6 -0.3996083016015106 2226 7 0.033377609530418795 2226 8 0.2705699670097417 2226 9 0.22725083654927403 2226 10-11 0.22406386662019884 2226 12-13 0.1625422560811174 2226 14-15 0.16075303825201814 2226 16-17 0.13108244983978068 2226 18-19 0.25132843998390086 2226 20-21 0.03784819011524121 2226 22-23 0.15436435716268448 2226 24-25 0.2760360765511649 2226 26-27 0.1337392072656911 2226 28-29 0.15739832691822642 2226 30-31 0.2669048085880803 2226 32-33 0.19215502763891124 2226 34-35 0.16500507285481092 2226 36-37 0.2803746588555427 2226 38-39 0.22802928264639633 2226 40-41 0.16947616776417362 2226 42-43 0.3062476712987774 2226 44-45 0.06192903028199481 2226 46-47 0.22256784408593688 2226 48-49 0.2175231259240391 2226 50-51 0.1808235832991798 2226 52-53 0.14897690905186067 2226 54-55 0.40081922466657005 2226 56-57 0.40517646017791265 2226 58-59 0.4524270705210043 2226 60-61 0.3261091618364773 2226 62-63 0.3937101508424661 2226 64-65 0.3746167458282841 2226 66-67 0.45164514763030894 2226 68-69 0.3581669841420876 2226 70-71 0.4029369978251154 2226 72-73 0.34965645328093586 2226 74-75 0.4400507332569745 2226 76-77 0.41309865563194137 2226 78-79 0.39051396212119016 2226 80-81 0.33371282779608435 2226 82-83 0.2651703818932347 2226 84-85 0.2707490936565904 2226 86-87 0.3126985662739372 2226 88-89 0.3333916902127285 2226 90-91 0.42062804207164817 2226 92-93 0.27650699766147824 2226 94-95 0.5013279019532959 2226 96-97 0.5329619797527769 2226 98-99 0.5822753985246862 2226 100 0.506700923084999 2227 1 1.2251394385248329 2227 2 1.6578770625856887 2227 3 0.7260235459748774 2227 4 -0.0021602506451028347 2227 5 3.2738562171723835 2227 6 0.22582480473418443 2227 7 0.33855976092797135 2227 8 0.5407099723845334 2227 9 0.29267355217815805 2227 10-11 0.230884120921921 2227 12-13 0.2609658622402904 2227 14-15 0.2748779195656752 2227 16-17 0.32746025742058293 2227 18-19 0.26413759235088463 2227 20-21 0.2765490441441827 2227 22-23 0.2523013261895173 2227 24-25 0.4133609594598866 2227 26-27 0.28790184888642756 2227 28-29 0.31701295645235916 2227 30-31 0.3563345288294073 2227 32-33 0.2078155716784309 2227 34-35 0.275149484595687 2227 36-37 0.30726645180646983 2227 38-39 0.15523017633832836 2227 40-41 0.25665691806833735 2227 42-43 0.47340040543184614 2227 44-45 0.32533992790604316 2227 46-47 0.39534389316494156 2227 48-49 0.30968126120232853 2227 50-51 0.39242158314508657 2227 52-53 0.35689721677400854 2227 54-55 0.42666105207516125 2227 56-57 0.5149745150250666 2227 58-59 0.5546739184525293 2227 60-61 0.4380431791392283 2227 62-63 0.48361632543738864 2227 64-65 0.4079978883759381 2227 66-67 0.5140110358057441 2227 68-69 0.3034033591924796 2227 70-71 0.5180005461973778 2227 72-73 0.42260153648705057 2227 74-75 0.5182812482962262 2227 76-77 0.6359365205866396 2227 78-79 0.565940168378134 2227 80-81 0.41636736855348033 2227 82-83 0.4641768417626224 2227 84-85 0.40884936312282605 2227 86-87 0.43750333107382033 2227 88-89 0.32391109595430834 2227 90-91 0.3913139583202181 2227 92-93 0.3918102329795161 2227 94-95 0.5239575967305612 2227 96-97 0.5235384945051962 2227 98-99 0.7021947794003509 2227 100 0.5907666688012156 2228 1 1.3904933707742142 2228 2 1.6387582040249296 2228 3 -0.18740459852306657 2228 4 -0.11720147244516177 2228 5 3.090841517186721 2228 6 0.04462757207620882 2228 7 0.10848532253238119 2228 8 0.3164503144269162 2228 9 0.3660651243274273 2228 10-11 0.19543864772928288 2228 12-13 0.16150662810107264 2228 14-15 0.15278940173072186 2228 16-17 0.13200661320132667 2228 18-19 0.1737889242382309 2228 20-21 0.13803649119725492 2228 22-23 0.26025399656136017 2228 24-25 0.19609063127938242 2228 26-27 0.2642194519452161 2228 28-29 0.28083665097146593 2228 30-31 0.2854055577226191 2228 32-33 0.09173295853951657 2228 34-35 0.2689693952261507 2228 36-37 0.2102646725068169 2228 38-39 0.1847025518784875 2228 40-41 0.21846137122066978 2228 42-43 0.3959692069483651 2228 44-45 0.23901330326765446 2228 46-47 0.1862017780555263 2228 48-49 0.14794046372099245 2228 50-51 0.09711008848619684 2228 52-53 0.12706886694257236 2228 54-55 0.15404795747608802 2228 56-57 0.31387148361135786 2228 58-59 0.30637527457705716 2228 60-61 0.33074599206803157 2228 62-63 0.18487202753742338 2228 64-65 0.24672382290851402 2228 66-67 0.19335785114240167 2228 68-69 0.19332186192189482 2228 70-71 0.2819683772829862 2228 72-73 0.30634881275194914 2228 74-75 0.21736253671674177 2228 76-77 0.2523745076264987 2228 78-79 0.2986218732980106 2228 80-81 0.21428527203615744 2228 82-83 0.07634826921495375 2228 84-85 0.2092357286012927 2228 86-87 0.18920618817420376 2228 88-89 0.28008174347100123 2228 90-91 0.41483146234318724 2228 92-93 0.2807646803638093 2228 94-95 0.5182147916582878 2228 96-97 0.20030484645773328 2228 98-99 0.47113398544702534 2228 100 0.5656366163914299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 23.0 13 29.0 14 118.0 15 500.0 16 1929.0 17 4942.0 18 7948.0 19 10398.0 20 12031.0 21 13895.0 22 16167.0 23 18131.0 24 20543.0 25 23767.0 26 26345.0 27 29951.0 28 33979.0 29 38829.0 30 44372.0 31 51605.0 32 59647.0 33 70947.0 34 88008.0 35 113049.0 36 158811.0 37 263306.0 38 645327.0 39 1201964.0 40 1313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.543489445774537 19.179321599556076 19.619090521739196 35.65809843293019 2 18.560993850643808 51.77323116413114 12.277310674346486 17.38846431087856 3 41.38137730004726 14.929675841499671 24.01961679233125 19.669330066121816 4 17.545304499109836 15.738838097904104 22.532129495712123 44.18372790727394 5 16.767313279740787 35.4086752850189 26.47039731915558 21.353614116084728 6 17.560957633759923 28.373757638087355 30.469722510154252 23.595562217998467 7 20.140005963742876 26.746845876463976 36.32808564529794 16.78506251449521 8 20.86268718681053 27.64495715503771 34.3070394479278 17.185316210223963 9 20.745001308372164 27.904366446981854 28.01708253968898 23.333549704957008 10-11 19.508234630684065 30.077802502743523 28.694173585487416 21.719789281084996 12-13 24.15150205857315 26.743718630340574 27.383840555750517 21.72093875533576 14-15 21.70031583495443 27.41065035224624 28.667887814017774 22.22114599878156 16-17 20.827087293103087 29.738031437444594 28.281360193165767 21.153521076286548 18-19 21.35699492270462 29.55198564915206 29.7900451472916 19.300974280851722 20-21 22.785149063144676 28.899489971513326 29.91694372376917 18.398417241572833 22-23 21.84393250016735 30.399689101023235 28.666231218774023 19.09014718003539 24-25 22.20114852762491 28.458582076180395 26.630579936805965 22.709689459388738 26-27 20.19396363739632 26.536593512773027 30.018215786067966 23.251227063762688 28-29 19.645419623123118 27.977957055136944 30.7094227672855 21.667200554454443 30-31 20.021906419791446 30.119450679364434 28.240566909336874 21.618075991507247 32-33 20.74353065749251 29.956144176526788 28.89592322052934 20.40440194545136 34-35 21.45302673474259 29.149585141219674 29.90861003545114 19.4887780885866 36-37 21.80772406126833 28.260382288089353 28.808782929901678 21.12311072074064 38-39 21.42200054498971 29.42912327653765 28.945799894112422 20.203076284360215 40-41 20.848880305290603 29.945272517506456 29.729439159521103 19.476408017681837 42-43 20.473282819540533 30.00251532182596 29.304327570631862 20.219874288001645 44-45 20.59252692981513 30.145553867406115 28.862842027753715 20.399077175025035 46-47 19.88769370789951 28.049120225680944 29.024320069889686 23.038865996529857 48-49 20.82090041698869 28.25921590980549 30.2503757766558 20.669507896550023 50-51 21.902048565963256 30.804980198615628 28.095111556476038 19.197859678945083 52-53 20.32687443814441 30.309081006263703 28.363535060972 21.00050949461988 54-55 22.470948678181976 28.342146806074915 28.262395261639 20.92450925410411 56-57 21.411731888437593 28.835052809981278 29.245732302633527 20.507482998947605 58-59 21.21064780924101 27.883635148847453 29.15584527560692 21.749871766304615 60-61 19.620202666313258 30.385561622615214 29.588247206517398 20.405988504554127 62-63 20.25072602789286 31.224609310942135 27.470565819935615 21.05409884122939 64-65 19.97841014337849 30.220694246080075 28.74568038053052 21.055215230010916 66-67 20.18495378775431 30.22745390777295 29.450747394919457 20.136844909553282 68-69 20.85918805195894 30.08686306448483 29.172422489937027 19.8815263936192 70-71 19.618685582955866 30.905204210862262 29.220903256866247 20.255206949315625 72-73 19.96770315436019 30.853241213114558 28.644644768506026 20.534410864019225 74-75 20.05763261044926 29.27176749246249 30.395344764516675 20.27525513257157 76-77 19.516517606902795 30.529782539756596 28.736298435971243 21.217401417369366 78-79 19.61959840074324 29.524465883266153 28.532165332262295 22.323770383728313 80-81 21.050478536758362 29.43884300566184 28.188839202250538 21.321839255329255 82-83 20.27158695738899 28.014208854062073 29.990087474990485 21.724116713558455 84-85 20.788647521834942 28.942984048678206 28.7252770063904 21.543091423096453 86-87 21.11543628971349 28.622077884318266 29.287505147278083 20.974980678690166 88-89 20.359386505307526 28.665436729218353 28.9432207051416 22.031956060332522 90-91 20.569300788336488 28.94465754795505 28.52209052853502 21.96395113517344 92-93 20.424636073071404 28.480979920037164 29.418393075567113 21.675990931324325 94-95 20.857176472020107 28.95791030990502 28.855962086282243 21.328951131792632 96-97 20.44226697959413 28.718075888290034 29.493311750263874 21.346345381851968 98-99 21.75160267137816 29.43568590142785 28.222263693450095 20.5904477337439 100 21.00924515378275 30.999697755888185 27.23212685868296 20.758930231646108 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 13.5 2 15.0 3 14.0 4 30.5 5 38.5 6 38.5 7 42.5 8 53.0 9 61.0 10 78.5 11 90.0 12 104.0 13 126.5 14 145.0 15 184.5 16 257.0 17 349.0 18 518.0 19 816.0 20 1299.0 21 2047.5 22 3046.5 23 4676.0 24 7350.0 25 11058.0 26 15714.5 27 21367.5 28 27641.0 29 34709.0 30 42958.5 31 52220.0 32 62814.0 33 74722.5 34 87464.0 35 101320.5 36 115058.0 37 126135.0 38 134444.5 39 140916.5 40 144877.5 41 145529.0 42 143035.5 43 140469.5 44 139237.0 45 134874.5 46 125239.0 47 114933.5 48 107749.5 49 103583.0 50 99538.5 51 91062.0 52 78878.5 53 66562.0 54 55306.0 55 45180.0 56 35604.0 57 26335.5 58 18309.5 59 12812.0 60 9731.5 61 8109.0 62 6976.5 63 5356.5 64 3846.0 65 3413.0 66 3922.5 67 3646.5 68 2398.0 69 2126.5 70 2741.5 71 2646.0 72 1653.0 73 731.0 74 297.5 75 172.5 76 129.5 77 93.0 78 75.5 79 66.5 80 56.0 81 37.0 82 29.5 83 29.5 84 23.5 85 21.0 86 20.5 87 20.5 88 18.5 89 13.5 90 9.0 91 10.5 92 13.0 93 12.5 94 12.0 95 13.5 96 18.0 97 23.0 98 48.5 99 108.0 100 110.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.291758878167224 2 0.005612138989017111 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.8879276128733005E-4 30-31 0.00992266742937664 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.761613239779652E-4 40-41 0.0012339944162597866 42-43 6.761613239779653E-5 44-45 0.0 46-47 0.0023665646339228784 48-49 0.0 50-51 0.0 52-53 0.0013861307141548288 54-55 0.01908465336927807 56-57 0.011376414275929265 58-59 0.012948489354178036 60-61 2.3665646339228783E-4 62-63 0.011596166706222103 64-65 1.1832823169614392E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0142419859669479E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2957874.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.99122105291646 #Duplication Level Percentage of deduplicated Percentage of total 1 85.33312675789224 62.28569118321852 2 8.959845568327172 13.079801369555247 3 2.6340436384258394 5.767861844261063 4 1.0821496824261776 3.1594970672924987 5 0.5736304661174724 2.0934994077533964 6 0.35975874533002805 1.575553806366237 7 0.21125530006704057 1.0793847614055487 8 0.16547023970334523 0.9662299875092755 9 0.1221798111917291 0.8026248246209184 >10 0.5241183113439437 6.431611298677989 >50 0.02387783915347745 1.1950780939554972 >100 0.00988343502670726 1.2429493293171783 >500 6.60204994926345E-4 0.32021702606659136 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.6904033099449132E-5 0.0 0.0 0.0 0.0 12-13 3.3808066198898264E-5 0.0 0.0 0.0 0.0 14-15 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 16-17 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 18-19 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 20-21 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 22-23 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 24-25 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 26-27 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 28-29 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 30-31 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 32-33 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 34-35 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 36-37 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 38-39 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 40-41 3.3808066198898264E-5 0.0 0.0 3.3808066198898264E-5 0.0 42-43 5.0712099298347395E-5 0.0 0.0 3.3808066198898264E-5 0.0 44-45 6.761613239779653E-5 0.0 0.0 3.3808066198898264E-5 0.0 46-47 1.0142419859669479E-4 0.0 0.0 3.3808066198898264E-5 0.0 48-49 1.0142419859669479E-4 0.0 0.0 3.3808066198898264E-5 0.0 50-51 1.0142419859669479E-4 0.0 0.0 3.3808066198898264E-5 0.0 52-53 1.1832823169614392E-4 0.0 0.0 3.3808066198898264E-5 0.0 54-55 1.3523226479559305E-4 0.0 0.0 3.3808066198898264E-5 0.0 56-57 1.690403309944913E-4 0.0 2.873685626906352E-4 3.3808066198898264E-5 0.0 58-59 2.5356049649173694E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 60-61 3.3808066198898266E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 62-63 3.8879276128733005E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 64-65 5.07120992983474E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 66-67 5.240250260829232E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 68-69 5.747371253812704E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 70-71 5.916411584807196E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 72-73 6.085451915801688E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 74-75 6.59257290878516E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 76-77 7.606814894752108E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 78-79 8.621056880719057E-4 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 80-81 0.0010649540852652952 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 82-83 0.001284706515558134 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 84-85 0.001403034747254278 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 86-87 0.0014537468465526252 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 88 0.0014537468465526252 0.0 5.747371253812704E-4 3.3808066198898264E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11270 0.0 79.59334 8 CACCGGC 11135 0.0 77.05945 9 CTTCACC 12655 0.0 74.40613 6 CCTTCAC 13875 0.0 71.5851 5 GTGTGGC 4700 0.0 69.11116 7 ATGCAGC 85 0.0 66.268875 2 TTCACCG 14080 0.0 65.975555 7 CGTAGTG 2410 0.0 64.663765 4 GGCGCGC 430 0.0 64.40558 6 GCGTAGT 1555 0.0 63.693043 3 CGGGCCC 645 0.0 63.31396 7 GCCTTCA 4285 0.0 62.87833 4 ACGCAGC 60 3.6561687E-10 62.58727 2 CGGGGCA 1285 0.0 62.464447 94 TGTGGCA 5540 0.0 59.31004 8 GCTGCCT 145 0.0 58.399868 1 AGGCGCG 490 0.0 57.47714 5 ACCTTCA 5615 0.0 56.511505 4 AGGCTGC 75 4.1836756E-11 56.328545 2 AGCGTAG 1435 0.0 55.936012 2 >>END_MODULE