FastQCFastQC Report
Tue 7 Mar 2017
1HL_25A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_25A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2957874
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG112700.079.593348
CACCGGC111350.077.059459
CTTCACC126550.074.406136
CCTTCAC138750.071.58515
GTGTGGC47000.069.111167
ATGCAGC850.066.2688752
TTCACCG140800.065.9755557
CGTAGTG24100.064.6637654
GGCGCGC4300.064.405586
GCGTAGT15550.063.6930433
CGGGCCC6450.063.313967
GCCTTCA42850.062.878334
ACGCAGC603.6561687E-1062.587272
CGGGGCA12850.062.46444794
TGTGGCA55400.059.310048
GCTGCCT1450.058.3998681
AGGCGCG4900.057.477145
ACCTTCA56150.056.5115054
AGGCTGC754.1836756E-1156.3285452
AGCGTAG14350.055.9360122
Produced by FastQC (version 0.11.5)