FastQCFastQC Report
Tue 7 Mar 2017
1HL_26A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_26A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3142369
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG114850.079.123128
CACCGGC117050.074.107069
CTTCACC131500.071.960456
CCTTCAC144600.069.693595
GTGTGGC50250.067.068337
CGTAGTG25600.066.740664
TTCACCG142200.066.2156147
GGCGCGC8100.064.9025966
GCGCGCA8150.064.504427
AGGCGCG8450.062.2143255
ATGCTGC1000.061.0219732
ACCTTCA56350.060.3078354
CGGGCCC9350.060.241917
GCTGCAA1100.059.741792
CAGCTGC802.3646862E-1159.553641
CGGGGCA12250.059.39155694
GCCTTCA40550.057.993054
CAGCGTA12600.057.978141
GCGTAGT16800.057.834923
TGTGGCA57500.057.6322988
Produced by FastQC (version 0.11.5)