##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_26A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3142369 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.75702789837858 12.0 12.0 12.0 2.0 27.0 2 14.57692365218725 12.0 12.0 12.0 12.0 22.0 3 13.685088543070531 12.0 12.0 12.0 12.0 22.0 4 13.698417658779093 12.0 12.0 12.0 12.0 22.0 5 22.444422663283657 22.0 22.0 22.0 22.0 27.0 6 26.775444577005437 27.0 22.0 32.0 22.0 32.0 7 34.946805737963935 37.0 37.0 37.0 27.0 41.0 8 37.59766787414209 41.0 37.0 41.0 27.0 41.0 9 38.54810049360849 41.0 41.0 41.0 32.0 41.0 10-11 38.9048396289551 41.0 41.0 41.0 37.0 41.0 12-13 39.08939306618669 41.0 41.0 41.0 37.0 41.0 14-15 39.040487288412024 41.0 41.0 41.0 37.0 41.0 16-17 38.94914219176678 41.0 41.0 41.0 37.0 41.0 18-19 38.872130548640214 41.0 41.0 41.0 37.0 41.0 20-21 38.87878396840091 41.0 41.0 41.0 37.0 41.0 22-23 38.873275385545114 41.0 41.0 41.0 37.0 41.0 24-25 38.89036424430103 41.0 41.0 41.0 37.0 41.0 26-27 38.86396362107696 41.0 41.0 41.0 37.0 41.0 28-29 38.8111214819138 41.0 41.0 41.0 37.0 41.0 30-31 38.68817522703412 41.0 41.0 41.0 32.0 41.0 32-33 38.72416463502536 41.0 41.0 41.0 32.0 41.0 34-35 38.69487924556282 41.0 41.0 41.0 32.0 41.0 36-37 38.625194717743206 41.0 41.0 41.0 32.0 41.0 38-39 38.51916181708768 41.0 41.0 41.0 32.0 41.0 40-41 38.57871608967629 41.0 41.0 41.0 32.0 41.0 42-43 38.32107670995991 41.0 41.0 41.0 32.0 41.0 44-45 38.422508941502414 41.0 41.0 41.0 32.0 41.0 46-47 38.44409568067913 41.0 41.0 41.0 32.0 41.0 48-49 38.37677067842765 41.0 41.0 41.0 32.0 41.0 50-51 38.27447142585737 41.0 41.0 41.0 32.0 41.0 52-53 38.23782630238524 41.0 41.0 41.0 32.0 41.0 54-55 38.11990157744046 41.0 37.0 41.0 32.0 41.0 56-57 37.90989377759264 41.0 37.0 41.0 29.5 41.0 58-59 37.89857317838866 41.0 37.0 41.0 29.5 41.0 60-61 37.88821570604853 41.0 37.0 41.0 29.5 41.0 62-63 37.87855722863865 41.0 37.0 41.0 29.5 41.0 64-65 37.86164546557072 41.0 37.0 41.0 29.5 41.0 66-67 37.72429240487034 41.0 37.0 41.0 27.0 41.0 68-69 37.755330452916255 41.0 37.0 41.0 27.0 41.0 70-71 37.7239744918563 41.0 37.0 41.0 27.0 41.0 72-73 37.57177848941356 41.0 37.0 41.0 27.0 41.0 74-75 37.51015507726814 41.0 37.0 41.0 27.0 41.0 76-77 36.424198431183605 39.0 34.5 41.0 24.5 41.0 78-79 36.969909962833775 41.0 37.0 41.0 27.0 41.0 80-81 37.635796750795336 41.0 37.0 41.0 27.0 41.0 82-83 37.81793290348779 41.0 37.0 41.0 29.5 41.0 84-85 37.73383854665063 41.0 37.0 41.0 27.0 41.0 86-87 37.582194357187205 41.0 37.0 41.0 27.0 41.0 88-89 37.5053096883275 41.0 37.0 41.0 27.0 41.0 90-91 37.3350645643462 41.0 37.0 41.0 27.0 41.0 92-93 37.21893832328412 41.0 37.0 41.0 27.0 41.0 94-95 37.05375928161206 41.0 37.0 41.0 27.0 41.0 96-97 36.96794997659409 41.0 37.0 41.0 27.0 41.0 98-99 36.85249408965019 41.0 37.0 41.0 27.0 41.0 100 36.713870013356164 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3372300145763294 1101 2 -0.6170590007132404 1101 3 -0.08709442449709925 1101 4 0.09715661727018343 1101 5 -1.0410408604555172 1101 6 -0.6912052533107875 1101 7 -0.04381081072055082 1101 8 0.04230880688074734 1101 9 0.09171440195380143 1101 10-11 0.1015139737771733 1101 12-13 0.06737055631847255 1101 14-15 0.05192204321176774 1101 16-17 0.006922784140279248 1101 18-19 0.16725005431420925 1101 20-21 0.12374787943888776 1101 22-23 0.03543727570740174 1101 24-25 0.07910524777907568 1101 26-27 0.14229445618256875 1101 28-29 0.20543199081243557 1101 30-31 0.16567189395287585 1101 32-33 0.1085668924367198 1101 34-35 0.18892872343142386 1101 36-37 0.276560690987516 1101 38-39 0.11249371509013883 1101 40-41 0.1670928757314769 1101 42-43 0.42902823287067093 1101 44-45 0.1385118680535271 1101 46-47 0.18967494518484074 1101 48-49 0.29895911916905504 1101 50-51 0.15280923487878084 1101 52-53 0.2692461237313637 1101 54-55 0.2644063346291503 1101 56-57 0.22935718424215423 1101 58-59 0.3127273540372997 1101 60-61 0.14797176671223866 1101 62-63 0.0894618183002649 1101 64-65 0.06739643501768455 1101 66-67 0.10554714633683915 1101 68-69 0.14140037185858745 1101 70-71 0.10813739628395069 1101 72-73 0.12042582070544938 1101 74-75 0.16061241396464254 1101 76-77 0.04610099362453468 1101 78-79 0.12978332743755772 1101 80-81 0.08685788750977252 1101 82-83 0.1464944278969469 1101 84-85 0.17034160467332526 1101 86-87 0.06492783399192348 1101 88-89 -0.1433167239936708 1101 90-91 -0.01077845237353614 1101 92-93 0.10296263549869167 1101 94-95 0.17719537198718882 1101 96-97 -0.056952165737712335 1101 98-99 -0.06528382967163537 1101 100 -0.3399672630527917 1102 1 1.453472361910615 1102 2 1.0074330472892825 1102 3 -0.013082284460645965 1102 4 0.0012388641280143275 1102 5 -0.8027564907198794 1102 6 -0.18421854377138658 1102 7 -0.040476925843741185 1102 8 -0.2022703241907351 1102 9 -0.1711104821243552 1102 10-11 -0.19630816778362714 1102 12-13 -0.2691261611292859 1102 14-15 -0.3194618401667455 1102 16-17 -0.24935145717257967 1102 18-19 -0.150440355213334 1102 20-21 -0.057375327990534686 1102 22-23 -0.06028706807529005 1102 24-25 -0.07837452859826755 1102 26-27 -0.068913384941645 1102 28-29 -0.11778208318732197 1102 30-31 -0.18820013666950786 1102 32-33 -0.2275660017464034 1102 34-35 -0.12991354338620198 1102 36-37 -0.15769771538190724 1102 38-39 -0.22428572267425295 1102 40-41 -0.17264887163641873 1102 42-43 -0.08051201438582467 1102 44-45 -0.00951886116946099 1102 46-47 -0.3286126295829561 1102 48-49 -0.3768697920450421 1102 50-51 -0.18844617361298788 1102 52-53 -0.17390162173697377 1102 54-55 -0.17328849158905513 1102 56-57 -0.023665317428758215 1102 58-59 -0.12083044011885846 1102 60-61 -0.10287873938317915 1102 62-63 -0.34931053712477933 1102 64-65 -0.15086449629169607 1102 66-67 -0.22099514510480844 1102 68-69 -0.41655071361805796 1102 70-71 -0.40265379065932194 1102 72-73 -0.2579742168168764 1102 74-75 -0.17867098774704715 1102 76-77 -0.10677317759454041 1102 78-79 -0.2349922616825353 1102 80-81 -0.3155079082827754 1102 82-83 -0.35382464217469334 1102 84-85 -0.5312307502305629 1102 86-87 -0.3506818452051945 1102 88-89 -0.44216955699456406 1102 90-91 -0.5733924687869418 1102 92-93 -0.2679556713583793 1102 94-95 -0.15207862308540854 1102 96-97 -0.2999926859430957 1102 98-99 -0.21776759931059786 1102 100 -0.45290725468844073 1103 1 1.353556182300844 1103 2 0.7679060806040408 1103 3 0.32579157889673205 1103 4 0.9656690493690689 1103 5 2.820504637557427 1103 6 -0.2564570030817066 1103 7 -0.6901909130117971 1103 8 -0.27411904878957927 1103 9 -0.3650115954705271 1103 10-11 -0.33840493517969605 1103 12-13 -0.4994705472034653 1103 14-15 -0.6584791280386142 1103 16-17 -0.5231604951914548 1103 18-19 -0.4643481848940496 1103 20-21 -0.26972314849314927 1103 22-23 -0.23249729737388947 1103 24-25 -0.5699981757150994 1103 26-27 -0.36239104811211575 1103 28-29 -0.375554400784317 1103 30-31 -0.3051730632855225 1103 32-33 -0.4881462689682152 1103 34-35 -0.5600690827232526 1103 36-37 -0.35402774696338923 1103 38-39 -0.23312887418713757 1103 40-41 -0.3764508922898173 1103 42-43 -0.31418172191086313 1103 44-45 -0.6839641447840847 1103 46-47 -0.6310497091012834 1103 48-49 -0.5695750767433694 1103 50-51 -0.5313349868353399 1103 52-53 -0.28353628391002417 1103 54-55 -0.41155641313103075 1103 56-57 -0.38420143344742286 1103 58-59 -0.6283267792482619 1103 60-61 -0.487990084034827 1103 62-63 -0.5516829953366624 1103 64-65 -0.5794657125904479 1103 66-67 -0.6913810600682524 1103 68-69 -0.5901173213941533 1103 70-71 -0.7677620313475941 1103 72-73 -0.6774104460900574 1103 74-75 -0.5791885602603841 1103 76-77 -0.5645703135193614 1103 78-79 -0.7645526963176863 1103 80-81 -0.6721019239216659 1103 82-83 -0.5737672723689045 1103 84-85 -0.7392302792843992 1103 86-87 -0.5375101364934309 1103 88-89 -0.4438028839281998 1103 90-91 -0.847777345528165 1103 92-93 -0.6186255989704748 1103 94-95 -0.5032374249287841 1103 96-97 -0.23968160726899157 1103 98-99 -0.39481753632824024 1103 100 -0.5729545098549664 1104 1 1.6620199521505832 1104 2 -0.38221204244655205 1104 3 -0.029025518983734955 1104 4 0.016715774795487803 1104 5 -1.1524741757370194 1104 6 -0.38037452279941775 1104 7 -0.29875984578728065 1104 8 -0.22356998137968276 1104 9 0.11917233699239915 1104 10-11 -0.06935590926376278 1104 12-13 -0.09509787921761159 1104 14-15 -0.1488906564603596 1104 16-17 -0.12007892238734996 1104 18-19 -0.029739357028219615 1104 20-21 -0.04017427939201923 1104 22-23 0.0680074469479024 1104 24-25 -0.11383404664626795 1104 26-27 -0.1831085351735453 1104 28-29 -0.05374951613750767 1104 30-31 -0.12220270775239328 1104 32-33 -0.10548241634612765 1104 34-35 -0.18168557403753738 1104 36-37 -0.15167331530124528 1104 38-39 -0.0964865192058113 1104 40-41 0.08829965629259817 1104 42-43 -0.07391542233097681 1104 44-45 -0.2929542816171349 1104 46-47 -0.26630167979469377 1104 48-49 -0.15041284507992714 1104 50-51 -0.31830980539358933 1104 52-53 -0.35586119019835394 1104 54-55 -0.33314556962898223 1104 56-57 -0.3184739986144862 1104 58-59 -0.2846550519357578 1104 60-61 -0.2977655613230894 1104 62-63 -0.24307440080696807 1104 64-65 -0.20479800103055368 1104 66-67 -0.5056606522635079 1104 68-69 -0.5345482937753516 1104 70-71 -0.36351283482145647 1104 72-73 -0.09160498915000659 1104 74-75 -0.37026959633749357 1104 76-77 -0.5161740144568085 1104 78-79 -0.4250746875748561 1104 80-81 -0.39586811195286487 1104 82-83 -0.3768673423158546 1104 84-85 -0.2615178544854615 1104 86-87 -0.2907919984619056 1104 88-89 -0.23633546746037837 1104 90-91 -0.29406321893818443 1104 92-93 -0.3159618217919373 1104 94-95 -0.13542834077042443 1104 96-97 -0.10116353692111346 1104 98-99 -0.23528877293431805 1104 100 -0.14621235652348474 1105 1 1.7054508676082065 1105 2 0.5506629687434614 1105 3 -0.032371463202297335 1105 4 -0.1489666911296208 1105 5 -1.1554070002630326 1105 6 0.4639961976709195 1105 7 -0.10145101806352841 1105 8 -0.2051015143178816 1105 9 -0.3022967973065036 1105 10-11 -0.13736107072264758 1105 12-13 -0.19274824958103665 1105 14-15 -0.09781279790629327 1105 16-17 -0.164262951791585 1105 18-19 -0.21382399568966548 1105 20-21 -0.1681724223702119 1105 22-23 -0.06797372988761907 1105 24-25 -0.250145133711861 1105 26-27 -0.26856022918548206 1105 28-29 -0.16263654696366103 1105 30-31 -0.19752587795093746 1105 32-33 -0.29148867963101566 1105 34-35 -0.22401882721914745 1105 36-37 -0.2210761224853357 1105 38-39 -0.27006087242302357 1105 40-41 -0.07817020926069063 1105 42-43 -0.1066518074673084 1105 44-45 -0.16430549138772932 1105 46-47 -0.4320502448706023 1105 48-49 -0.3191072463257143 1105 50-51 -0.24231302239241614 1105 52-53 -0.3098193582838036 1105 54-55 -0.08305364236511537 1105 56-57 -0.21787727161542847 1105 58-59 -0.22175409813021218 1105 60-61 -0.273948072528718 1105 62-63 -0.4186881340245492 1105 64-65 -0.3919438891429081 1105 66-67 -0.2181261583956342 1105 68-69 -0.30387839189355503 1105 70-71 -0.6160063862507528 1105 72-73 -0.4221468044096497 1105 74-75 -0.34968062167585146 1105 76-77 -0.48598628506152153 1105 78-79 -0.49455198642004206 1105 80-81 -0.5306858526526597 1105 82-83 -0.4536649919965967 1105 84-85 -0.2682126705424537 1105 86-87 -0.458865419004475 1105 88-89 -0.3543456774370881 1105 90-91 -0.5272573146705781 1105 92-93 -0.3888524001126612 1105 94-95 -0.3441019936489127 1105 96-97 -0.4359900214455834 1105 98-99 -0.502132736260215 1105 100 -0.7578356059369185 1106 1 1.3787288528917614 1106 2 -0.5305868793746686 1106 3 -0.0970668764825735 1106 4 -0.1511556690028435 1106 5 -1.1260277229877431 1106 6 -0.6500246847395701 1106 7 -0.3428659032132799 1106 8 -0.22034506769975337 1106 9 -0.3051821144070317 1106 10-11 -0.2053122049701841 1106 12-13 -0.1713503175795239 1106 14-15 -0.22310456767517195 1106 16-17 -0.22960998526622234 1106 18-19 -0.0724689488649517 1106 20-21 -0.22073137821461586 1106 22-23 -0.20280530949163023 1106 24-25 -0.29702151552371703 1106 26-27 -0.304123948295846 1106 28-29 -0.25057959420625053 1106 30-31 -0.27187916181858895 1106 32-33 -0.37203987114897785 1106 34-35 -0.3171723307486971 1106 36-37 -0.5363506284527588 1106 38-39 -0.17657654826038538 1106 40-41 -0.2419403268755005 1106 42-43 -0.15821304034975725 1106 44-45 -0.1594929342832856 1106 46-47 -0.32715887203637806 1106 48-49 -0.30487638488181545 1106 50-51 -0.3576061425007211 1106 52-53 -0.3689421787946401 1106 54-55 -0.3471171519098064 1106 56-57 -0.3186688360775989 1106 58-59 -0.24298311244005788 1106 60-61 -0.21995159393335229 1106 62-63 -0.524550360821948 1106 64-65 -0.705766529700341 1106 66-67 -0.6960868151666588 1106 68-69 -0.5126801998953354 1106 70-71 -0.5780176930260765 1106 72-73 -0.420196891730761 1106 74-75 -0.5299321455677131 1106 76-77 -0.5604751665570902 1106 78-79 -0.5385831354185981 1106 80-81 -0.44256261401260844 1106 82-83 -0.6061136158403855 1106 84-85 -0.8329604439229286 1106 86-87 -0.6432426807917011 1106 88-89 -0.7686372039444436 1106 90-91 -0.8348336332745205 1106 92-93 -0.4863341298198023 1106 94-95 -0.5346909420601804 1106 96-97 -0.2823012899654671 1106 98-99 -0.614224875506018 1106 100 -0.8840935949232644 1107 1 1.4535458415486264 1107 2 -0.5686011461026226 1107 3 -0.21353213590822762 1107 4 -0.19449203392971803 1107 5 -1.2012027540201835 1107 6 -0.21411578282275912 1107 7 -0.3696961364123297 1107 8 -0.3669550997949713 1107 9 -0.34404786641831464 1107 10-11 -0.320556968537403 1107 12-13 -0.32399405709512763 1107 14-15 -0.36762566204430414 1107 16-17 -0.27586200020012086 1107 18-19 -0.3213575046455617 1107 20-21 -0.36783252089929164 1107 22-23 -0.27916723839198454 1107 24-25 -0.15033287799850115 1107 26-27 -0.2688555766246594 1107 28-29 -0.29209145587280005 1107 30-31 -0.4107478512902034 1107 32-33 -0.20411175647657842 1107 34-35 -0.40295588001267646 1107 36-37 -0.3106966274931082 1107 38-39 -0.4321759401934884 1107 40-41 -0.4618186882819657 1107 42-43 -0.32777743389278413 1107 44-45 -0.17243433812709696 1107 46-47 -0.3483919825849924 1107 48-49 -0.3790780827591007 1107 50-51 -0.2856185080740872 1107 52-53 -0.4165369787082014 1107 54-55 -0.33769319922787844 1107 56-57 -0.3814322777321948 1107 58-59 -0.7098653273406157 1107 60-61 -0.4192608621123881 1107 62-63 -0.39920464191194327 1107 64-65 -0.3980738631898362 1107 66-67 -0.3312760519085671 1107 68-69 -0.6326628474472429 1107 70-71 -0.3834987544754753 1107 72-73 -0.44438549080424394 1107 74-75 -0.41726314389322994 1107 76-77 -0.4131650607606119 1107 78-79 -0.6047171552183457 1107 80-81 -0.5176301692967584 1107 82-83 -0.5123583917625965 1107 84-85 -0.4326835662239006 1107 86-87 -0.35256591514480107 1107 88-89 -0.43794233194334 1107 90-91 -0.3621554813480614 1107 92-93 -0.19231943848384958 1107 94-95 -0.27811317412156455 1107 96-97 -0.310626034904395 1107 98-99 -0.38253092659866894 1107 100 -0.5072590374800257 1108 1 1.7477693635578895 1108 2 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0.18431688825597803 2223 32-33 0.3069837422873434 2223 34-35 0.18655775983230427 2223 36-37 0.1519884650022547 2223 38-39 0.16561267210933295 2223 40-41 0.15354140023726615 2223 42-43 0.46168585002186546 2223 44-45 0.3388053507913398 2223 46-47 0.30253661212423566 2223 48-49 0.23450690548444442 2223 50-51 0.27854756459572627 2223 52-53 0.22718609073038465 2223 54-55 0.27170553351702864 2223 56-57 0.3504409780473523 2223 58-59 0.5120430675985546 2223 60-61 0.39888772871805855 2223 62-63 0.44241394662005007 2223 64-65 0.26467061213374166 2223 66-67 0.37087317156328226 2223 68-69 0.47755412175536094 2223 70-71 0.6042904065211374 2223 72-73 0.6003029004280833 2223 74-75 0.515705375495763 2223 76-77 0.6755325551016469 2223 78-79 0.6621714247949981 2223 80-81 0.519175742537513 2223 82-83 0.4650763231581081 2223 84-85 0.3589036304489426 2223 86-87 0.5367131138542973 2223 88-89 0.4031615084833575 2223 90-91 0.5482351102475107 2223 92-93 0.5531497288442395 2223 94-95 0.6233297894115353 2223 96-97 0.494305356878904 2223 98-99 0.5501742450552598 2223 100 0.7460070180715519 2224 1 1.176385407898314 2224 2 -0.9530435676671409 2224 3 -0.01798182464777831 2224 4 -0.301827196295374 2224 5 -1.0193815797004042 2224 6 0.5342714487253915 2224 7 0.14452688989763374 2224 8 -0.0056236874942072745 2224 9 0.26688404116448794 2224 10-11 0.2380864119397188 2224 12-13 0.21453380821652246 2224 14-15 0.1397215911787626 2224 16-17 0.17094379784078484 2224 18-19 0.13976152636401196 2224 20-21 0.04635993010108308 2224 22-23 0.15906322980905685 2224 24-25 0.1440225906773165 2224 26-27 0.06838147887806656 2224 28-29 -0.013569376447890136 2224 30-31 0.06170366816974848 2224 32-33 0.1653380018961812 2224 34-35 0.12544484771767372 2224 36-37 -0.056092859450188826 2224 38-39 0.07594867286742613 2224 40-41 0.11272144766738279 2224 42-43 0.1719778370247127 2224 44-45 0.39024770524046914 2224 46-47 0.16651091706849286 2224 48-49 0.07018310888035018 2224 50-51 0.155835344731436 2224 52-53 0.29727381899719774 2224 54-55 0.3367574896977814 2224 56-57 0.37272191298429647 2224 58-59 0.3090897820278329 2224 60-61 0.20776429320132905 2224 62-63 0.2995893108868728 2224 64-65 0.13056855508293097 2224 66-67 0.31059053592486663 2224 68-69 0.40966471223791956 2224 70-71 0.5231277950217077 2224 72-73 0.44151498687958224 2224 74-75 0.24930993995908324 2224 76-77 0.5431533934327675 2224 78-79 0.3957481845347459 2224 80-81 0.3815892424165668 2224 82-83 0.13297770242201779 2224 84-85 0.07812230524838526 2224 86-87 0.27539409766770717 2224 88-89 0.33677492557166744 2224 90-91 0.3406699811114038 2224 92-93 0.27403544020877035 2224 94-95 0.27679178920705283 2224 96-97 0.2126120866194796 2224 98-99 0.2544538034713568 2224 100 0.45550276756470964 2225 1 1.462604489170399 2225 2 1.4904193298593604 2225 3 0.8124068679317276 2225 4 0.9543565492876649 2225 5 3.063657289557547 2225 6 -0.2780960424406871 2225 7 0.0346328969647729 2225 8 0.39578974006763445 2225 9 0.1326084227899358 2225 10-11 0.13967651794678204 2225 12-13 0.028844762280122893 2225 14-15 0.1407816618501414 2225 16-17 0.22447736674537566 2225 18-19 0.0830477454452847 2225 20-21 0.08505250960638477 2225 22-23 0.2468724170882055 2225 24-25 0.12034767901653964 2225 26-27 0.19117299831269463 2225 28-29 0.09067090623762653 2225 30-31 0.10092628301073603 2225 32-33 0.017634821684168855 2225 34-35 0.07509149082721223 2225 36-37 0.10521456104451232 2225 38-39 0.0863727011359714 2225 40-41 0.1215553699288634 2225 42-43 0.14229585822612734 2225 44-45 0.30120176884470595 2225 46-47 0.10644024569040056 2225 48-49 0.13096049403936405 2225 50-51 0.08481060974909838 2225 52-53 0.2059310628134483 2225 54-55 0.19188116460944116 2225 56-57 0.2101777433729879 2225 58-59 0.24583889863560415 2225 60-61 0.257410936310869 2225 62-63 0.14375892219429431 2225 64-65 0.3063636080864711 2225 66-67 0.3434527267375884 2225 68-69 0.37662584297996915 2225 70-71 0.3204776183432614 2225 72-73 0.2280874250421263 2225 74-75 0.23817919790960929 2225 76-77 0.2943901425493749 2225 78-79 0.12154323753828322 2225 80-81 0.02222528481939179 2225 82-83 0.104002437404354 2225 84-85 0.20904103316358658 2225 86-87 0.17150717187264775 2225 88-89 0.14967245207343893 2225 90-91 0.19296680089939144 2225 92-93 0.23428279003208985 2225 94-95 0.3509967362035198 2225 96-97 0.2133188004004012 2225 98-99 0.4254785384536959 2225 100 0.5724638983067578 2226 1 1.479900975365311 2226 2 1.4061090303934858 2226 3 0.7347338118037481 2226 4 1.068740579117181 2226 5 3.090612239386406 2226 6 -0.21449054331621298 2226 7 0.2005450977852803 2226 8 0.19490112364805867 2226 9 0.10329528463581994 2226 10-11 0.12947977356342477 2226 12-13 0.2508923691589402 2226 14-15 0.17093803517729356 2226 16-17 0.19201013754427265 2226 18-19 0.17602970246590388 2226 20-21 0.08384628740318334 2226 22-23 0.15668521472561991 2226 24-25 0.28219766032272986 2226 26-27 0.18448080653067223 2226 28-29 0.14901255841711958 2226 30-31 0.1489253539854971 2226 32-33 0.08449077619466294 2226 34-35 0.1591140820019774 2226 36-37 0.30272822262504206 2226 38-39 0.2990535180235838 2226 40-41 0.2536881248663576 2226 42-43 0.34326856527727756 2226 44-45 0.2970535074940486 2226 46-47 0.350833427299726 2226 48-49 0.20017476275928914 2226 50-51 0.3172062108523477 2226 52-53 0.3187343020651383 2226 54-55 0.23600193285540882 2226 56-57 0.388425146067668 2226 58-59 0.36672239156909825 2226 60-61 0.41440770486399714 2226 62-63 0.4443082807514571 2226 64-65 0.3828806068565811 2226 66-67 0.46659848425192507 2226 68-69 0.609507294072074 2226 70-71 0.5883806145126087 2226 72-73 0.3779138307592831 2226 74-75 0.5346512937930683 2226 76-77 0.5462048259317811 2226 78-79 0.467844760359192 2226 80-81 0.35022450685516304 2226 82-83 0.3510172199834969 2226 84-85 0.4282391257665026 2226 86-87 0.5255458510454858 2226 88-89 0.5082471158682651 2226 90-91 0.57521662812335 2226 92-93 0.5454793695071842 2226 94-95 0.6320162344040057 2226 96-97 0.6338508876482081 2226 98-99 0.4810168758509903 2226 100 0.5314158369860564 2227 1 1.6135120770181484 2227 2 1.770560521392909 2227 3 0.6855890458401177 2227 4 -0.11410193757864917 2227 5 3.1960338036741796 2227 6 -0.06017666426816248 2227 7 0.11155103007093459 2227 8 0.32343575812361536 2227 9 0.13395228793967107 2227 10-11 0.12950969417660474 2227 12-13 0.22665190805370372 2227 14-15 0.14655559409231955 2227 16-17 0.19857456761440773 2227 18-19 0.21745215509731253 2227 20-21 0.13498067885475962 2227 22-23 0.1182046380950581 2227 24-25 0.1711157639834937 2227 26-27 0.192197783702575 2227 28-29 0.23652450760467758 2227 30-31 0.18602316631222493 2227 32-33 0.15080298629504796 2227 34-35 0.23011283842252084 2227 36-37 0.15786360355319573 2227 38-39 0.08345561650673972 2227 40-41 0.1565180431417943 2227 42-43 0.4431221639075602 2227 44-45 0.19779749529304524 2227 46-47 0.18158604951431556 2227 48-49 0.1708397931728598 2227 50-51 0.17786394597246868 2227 52-53 0.31148068148037567 2227 54-55 0.14491443862989684 2227 56-57 0.4224498382601567 2227 58-59 0.36540131073744675 2227 60-61 0.2893674295587729 2227 62-63 0.23442432699208382 2227 64-65 0.3086020811411174 2227 66-67 0.36445414823035804 2227 68-69 0.3466865336641476 2227 70-71 0.3852545901604074 2227 72-73 0.395497813029003 2227 74-75 0.37189541855351393 2227 76-77 0.6410290934675444 2227 78-79 0.458147432454183 2227 80-81 0.3523521102026166 2227 82-83 0.23084193474031878 2227 84-85 0.2898652469318961 2227 86-87 0.25140779339768216 2227 88-89 0.22152626515217833 2227 90-91 0.30052831070617714 2227 92-93 0.4380638599826412 2227 94-95 0.5104485289478546 2227 96-97 0.41604584858497873 2227 98-99 0.42395585253806445 2227 100 0.48853457976635895 2228 1 1.1218674876560133 2228 2 1.6335896444077669 2228 3 -0.1939334634969434 2228 4 -0.1918189732129445 2228 5 3.1824237775819846 2228 6 0.1530961060659486 2228 7 0.2930516341820919 2228 8 0.36943407872963974 2228 9 0.3319820794074033 2228 10-11 0.3549362271994312 2228 12-13 0.36552330673251987 2228 14-15 0.3228008316784994 2228 16-17 0.27056637944216533 2228 18-19 0.21724423370537238 2228 20-21 0.2936280807261298 2228 22-23 0.32379420602413234 2228 24-25 0.3145356465260605 2228 26-27 0.3793078841507622 2228 28-29 0.3521122153821139 2228 30-31 0.30132559242065327 2228 32-33 0.1969329946219105 2228 34-35 0.22031900230914658 2228 36-37 0.36145250703510357 2228 38-39 0.2869479448207528 2228 40-41 0.2633436981129549 2228 42-43 0.44230230770254053 2228 44-45 0.3977534074458191 2228 46-47 0.300105123454216 2228 48-49 0.2932156774959651 2228 50-51 0.39566179180169314 2228 52-53 0.32917590602295377 2228 54-55 0.24591090909854785 2228 56-57 0.4652333887113258 2228 58-59 0.4837319911223332 2228 60-61 0.4553276934149011 2228 62-63 0.5051031171073959 2228 64-65 0.38898022268188015 2228 66-67 0.44493405984436407 2228 68-69 0.38198597802207956 2228 70-71 0.5502673158008022 2228 72-73 0.5792908725353243 2228 74-75 0.4548494820710616 2228 76-77 0.4956883477345073 2228 78-79 0.5186268959293656 2228 80-81 0.5009717174527495 2228 82-83 0.44845526754622966 2228 84-85 0.408935857086675 2228 86-87 0.5289026748511887 2228 88-89 0.46209538393100047 2228 90-91 0.42364224712253673 2228 92-93 0.44639310832615564 2228 94-95 0.5378645479829984 2228 96-97 0.41365243677643804 2228 98-99 0.5798190980946103 2228 100 0.597525571424967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 31.0 13 46.0 14 152.0 15 568.0 16 2042.0 17 4921.0 18 8224.0 19 10769.0 20 12617.0 21 14784.0 22 16768.0 23 19168.0 24 21969.0 25 24950.0 26 28155.0 27 31627.0 28 35838.0 29 40951.0 30 46794.0 31 53720.0 32 63325.0 33 76102.0 34 93790.0 35 121112.0 36 170585.0 37 281218.0 38 696125.0 39 1264751.0 40 1267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.514661624585315 18.63675012589052 18.90626172091893 36.942326528605236 2 18.627477813216657 50.55779638761041 12.677957320158642 18.136768479014293 3 40.137965974078796 16.590444979567962 23.319890184761878 19.951698861591368 4 17.483911023816745 15.615893614021777 23.288639876475358 43.61155548568612 5 17.537692104269105 35.825296138041075 26.03395718325887 20.603054574430946 6 18.022962930196933 27.985032948071982 30.72961832299135 23.26238579873974 7 20.452403902915282 26.795102675720134 35.81762676502983 16.93486665633476 8 20.879152002836076 27.775350380556834 33.76268032175725 17.582817294849843 9 21.135550917158362 27.988533491770063 27.657286588557866 23.21862900251371 10-11 19.852092481818655 29.81821994807102 28.677010879371583 21.65267669073874 12-13 24.275745464647848 26.733986365064066 27.18237418966391 21.807893980624172 14-15 21.914358243732675 27.367871182537762 28.484242302543084 22.233528271186483 16-17 21.02746367469893 29.342973406369527 28.34477745929902 21.284785459632527 18-19 21.532560307207717 29.433398814715904 29.55162172233751 19.48241915573887 20-21 22.85447698853954 28.791685508608317 29.801035460825894 18.552802042026254 22-23 21.91335581531004 30.119569025789144 28.536241288021873 19.430833870878946 24-25 22.136785972621293 28.318539293125667 26.63662351557058 22.908051218682466 26-27 20.45630223566997 26.492273822711464 29.83537261219163 23.21605132942694 28-29 19.980517802565974 27.77104185521946 30.48042615158817 21.7680141906264 30-31 20.234513246171275 29.87630647604741 28.106644091100048 21.782536186681263 32-33 21.104316520434104 29.794989067165567 28.546392864746313 20.554301547654017 34-35 21.587646135765723 28.897720159535687 29.79527547528632 19.71935822941227 36-37 22.070243819233195 28.030349077399887 28.65825114746231 21.241155955904606 38-39 21.536938175183863 29.374254237727733 28.81942858797676 20.269378999111648 40-41 21.037119236283896 29.540041997639005 29.707211504113438 19.715627261963657 42-43 20.82435389407465 29.586308269824883 29.096501869293306 20.492835966807156 44-45 20.675929529600122 30.032341841457832 28.603213053591098 20.688515575350955 46-47 20.14138396980041 27.829397885748435 28.847093874729733 23.18212426972142 48-49 21.058064959599744 28.32032589430552 29.92904874141909 20.692560404675646 50-51 22.20476016661315 30.441444018827834 28.018765460071688 19.33503035448733 52-53 20.430136099602493 30.14921088835008 28.2719738945388 21.148679117508625 54-55 22.52490783892708 28.200285697734596 27.987428790412576 21.287377672925746 56-57 21.46531422267013 28.7673946866271 29.073710807601046 20.693580283101724 58-59 21.34974487851354 27.796935295754892 28.870859656686033 21.982460169045538 60-61 19.68039030487875 30.238842476555867 29.418472844011706 20.66229437455368 62-63 20.41417010980925 30.923642756797115 27.31855948801939 21.343627645374244 64-65 20.204851980187545 30.010407761157076 28.70007365468032 21.084666603975062 66-67 20.297552578961923 30.034999072355923 29.25727373201556 20.4101746166666 68-69 20.994526740812425 29.794591278108967 29.00779634727812 20.203085633800484 70-71 19.840795272611206 30.44276467849575 29.318230927048987 20.39820912184406 72-73 20.203069722238222 30.643584505829836 28.344363758680153 20.808982013251786 74-75 20.20531325251745 29.05336706160225 30.242454021154103 20.4988656647262 76-77 19.771850473321244 30.156945285547305 28.613173691568367 21.458030549563084 78-79 19.70968399955575 29.355463982746777 28.336312508174565 22.59853950952291 80-81 21.309720961670894 29.09355822559067 28.098845327912365 21.497875484826075 82-83 20.658379076422914 27.756956614579636 29.785394395120367 21.79926991387708 84-85 20.858132829085317 29.04852994667399 28.034756580147018 22.058580644093677 86-87 21.39105878399386 28.35064882577444 28.25000819445457 22.008284195777136 88-89 20.590484440242378 28.431972820505802 28.31408405569174 22.66345868356008 90-91 20.994192598004883 28.71699663534104 27.872792787861645 22.416017978792432 92-93 21.337802785096212 28.13213534120277 28.612059882209888 21.91800199149113 94-95 21.60327128990898 28.749217548925664 28.416665897607825 21.230845263557526 96-97 21.601345990875036 28.70409235834493 28.154618378681818 21.539943272098217 98-99 22.516642698550044 29.394845099350203 27.432854002823987 20.65565819927577 100 21.84040130232955 30.73200505733095 26.34146403557316 21.08612960476634 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 6.5 2 16.5 3 21.5 4 26.0 5 41.5 6 46.5 7 47.5 8 58.0 9 69.0 10 79.5 11 98.5 12 106.5 13 121.5 14 159.0 15 205.5 16 282.0 17 371.5 18 523.0 19 835.5 20 1358.5 21 2099.5 22 3108.0 23 4802.0 24 7364.0 25 11010.0 26 16074.0 27 21668.5 28 27999.0 29 35816.0 30 44248.0 31 53547.0 32 64152.5 33 75865.0 34 88940.5 35 103240.5 36 117073.0 37 128088.0 38 136551.0 39 142782.0 40 146881.5 41 148606.0 42 148239.5 43 147631.0 44 146980.5 45 143413.0 46 133500.0 47 122754.0 48 117027.5 49 113031.0 50 108759.0 51 101584.0 52 89025.5 53 76045.0 54 64090.0 55 51893.5 56 40361.0 57 30053.5 58 21850.0 59 15920.5 60 12446.5 61 10681.0 62 9175.5 63 7351.5 64 5682.5 65 5332.5 66 6655.0 67 6363.5 68 4063.5 69 3428.5 70 4394.0 71 4323.5 72 2605.5 73 1090.0 74 539.0 75 326.5 76 219.5 77 170.5 78 131.0 79 91.5 80 58.5 81 55.0 82 48.5 83 33.0 84 29.0 85 28.0 86 23.5 87 19.5 88 22.0 89 23.0 90 15.0 91 13.5 92 15.5 93 13.0 94 14.5 95 17.5 96 21.5 97 24.5 98 49.0 99 93.5 100 94.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.335969773123399 2 0.005823631788628261 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.137006188643027E-4 30-31 0.01046980796971966 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.706052981047102E-4 40-41 0.0015752446641371526 42-43 1.5911562264011642E-4 44-45 0.0 46-47 0.002386734339601746 48-49 1.5911562264011643E-5 50-51 0.0 52-53 0.0012092787320648849 54-55 0.018680174097949667 56-57 0.01124947452065623 58-59 0.013827147607426118 60-61 1.5911562264011642E-4 62-63 0.011933671698008732 64-65 7.955781132005821E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5911562264011642E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3142369.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.29169304869775 #Duplication Level Percentage of deduplicated Percentage of total 1 85.72654079221384 62.83043313869603 2 8.84337357747208 12.962916435100954 3 2.4605903976633154 5.410225084323385 4 1.035435692199712 3.035553396974687 5 0.5592378681997354 2.0493745088651547 6 0.3449914739505145 1.5171005527919348 7 0.21307841891014856 1.0931814651845106 8 0.1491372877357822 0.8744419451877009 9 0.11387229932009449 0.7511304247666019 >10 0.5166885208398879 6.414477236343767 >50 0.0251669609684588 1.2587491954122523 >100 0.011268975022760024 1.4656361809206957 >500 5.736115391499934E-4 0.29697087950649964 >1k 4.4123964455397596E-5 0.039809555925756364 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 1.5911562264011643E-5 12-13 0.0 0.0 0.0 0.0 3.1823124528023286E-5 14-15 0.0 0.0 0.0 0.0 3.1823124528023286E-5 16-17 0.0 0.0 3.1823124528023286E-5 0.0 3.1823124528023286E-5 18-19 0.0 0.0 3.1823124528023286E-5 0.0 3.1823124528023286E-5 20-21 0.0 0.0 3.1823124528023286E-5 0.0 3.1823124528023286E-5 22-23 0.0 0.0 4.773468679203493E-5 0.0 3.1823124528023286E-5 24-25 0.0 0.0 6.364624905604657E-5 0.0 3.1823124528023286E-5 26-27 0.0 0.0 6.364624905604657E-5 0.0 3.1823124528023286E-5 28-29 1.5911562264011643E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 30-31 3.1823124528023286E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 32-33 4.773468679203493E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 34-35 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 36-37 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 38-39 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 40-41 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 42-43 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 44-45 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 46-47 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 48-49 6.364624905604657E-5 3.1823124528023286E-5 6.364624905604657E-5 0.0 3.1823124528023286E-5 50-51 7.955781132005821E-5 3.1823124528023286E-5 6.364624905604657E-5 3.1823124528023286E-5 3.1823124528023286E-5 52-53 9.546937358406985E-5 3.1823124528023286E-5 6.364624905604657E-5 3.1823124528023286E-5 3.1823124528023286E-5 54-55 9.546937358406985E-5 3.1823124528023286E-5 6.364624905604657E-5 3.1823124528023286E-5 3.1823124528023286E-5 56-57 1.432040603761048E-4 3.1823124528023286E-5 9.546937358406985E-5 3.1823124528023286E-5 3.1823124528023286E-5 58-59 1.909387471681397E-4 3.1823124528023286E-5 9.546937358406985E-5 3.1823124528023286E-5 3.1823124528023286E-5 60-61 2.3867343396017464E-4 3.1823124528023286E-5 9.546937358406985E-5 3.1823124528023286E-5 3.1823124528023286E-5 62-63 2.545849962241863E-4 3.1823124528023286E-5 9.546937358406985E-5 3.1823124528023286E-5 3.1823124528023286E-5 64-65 2.8640812075220954E-4 3.1823124528023286E-5 9.546937358406985E-5 3.1823124528023286E-5 3.1823124528023286E-5 66-67 2.8640812075220954E-4 3.1823124528023286E-5 9.546937358406985E-5 3.1823124528023286E-5 3.1823124528023286E-5 68-69 3.818774943362794E-4 3.1823124528023286E-5 1.1138093584808149E-4 3.1823124528023286E-5 3.1823124528023286E-5 70-71 3.818774943362794E-4 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 72-73 4.137006188643027E-4 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 74-75 4.137006188643027E-4 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 76-77 6.046393660324423E-4 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 78-79 7.796665509365705E-4 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 80-81 9.865168603687218E-4 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 82-83 0.0011933671698008733 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 84-85 0.0012888365433849432 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 3.1823124528023286E-5 86-87 0.0014002174792330246 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 9.546937358406985E-5 88 0.0014002174792330246 3.1823124528023286E-5 1.2729249811209314E-4 3.1823124528023286E-5 1.5911562264011642E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11485 0.0 79.12312 8 CACCGGC 11705 0.0 74.10706 9 CTTCACC 13150 0.0 71.96045 6 CCTTCAC 14460 0.0 69.69359 5 GTGTGGC 5025 0.0 67.06833 7 CGTAGTG 2560 0.0 66.74066 4 TTCACCG 14220 0.0 66.215614 7 GGCGCGC 810 0.0 64.902596 6 GCGCGCA 815 0.0 64.50442 7 AGGCGCG 845 0.0 62.214325 5 ATGCTGC 100 0.0 61.021973 2 ACCTTCA 5635 0.0 60.307835 4 CGGGCCC 935 0.0 60.24191 7 GCTGCAA 110 0.0 59.74179 2 CAGCTGC 80 2.3646862E-11 59.55364 1 CGGGGCA 1225 0.0 59.391556 94 GCCTTCA 4055 0.0 57.99305 4 CAGCGTA 1260 0.0 57.97814 1 GCGTAGT 1680 0.0 57.83492 3 TGTGGCA 5750 0.0 57.632298 8 >>END_MODULE