FastQCFastQC Report
Tue 7 Mar 2017
1HL_27A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_27A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3199649
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA141270.44151717891556225No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG81150.25362156911586237No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG74770.23368188198142986No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG68580.2143360099810948No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66800.20877289977744434No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA64920.20289725529268993No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT61450.19205231573838255No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA52180.16308038788004559No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50400.15751727767639512No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA49290.15404814715614118No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG47910.14973517407690656No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC46120.1441408104451457No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45920.14351574188293778No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC42200.13188946662587053No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT40420.12632635642222007No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA39650.12391984245771959No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38220.11945060223793297No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC37720.11788793083241317No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA36560.11426253317160726No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG36310.11348119746884737No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34380.10744928584354096No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG33580.1049490115947093No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32840.10263625791454001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC2350.085.999673
GCTGCAC550.085.999673
TGCTGCC202.8586717E-585.999672
CTGCAGC1800.085.999671
GCTGCTT500.085.9996643
GCTGCCT500.085.9996643
GCTGCCA258.3598025E-785.9996643
GCTGCAA700.085.9996643
TGCTGCA850.085.9996642
GCAGCTT600.085.9996643
GCAGCTC600.085.9996643
GCAGCCT357.2759576E-1085.9996643
CAGCTGC7300.084.8215941
AGCTGCC3100.084.612582
AGCAGCT1750.083.5425342
AGCTGCA1550.083.225492
AGCAGCG1450.083.0341642
CAGCAGC8000.082.774681
AGCTGCG1100.082.090592
CTGCTGC2400.080.624691
Produced by FastQC (version 0.11.5)