##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_27A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3199649 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.738613829204391 12.0 12.0 12.0 2.0 27.0 2 14.431998009781697 12.0 12.0 12.0 12.0 22.0 3 13.73493873859289 12.0 12.0 12.0 12.0 22.0 4 13.724848569327449 12.0 12.0 12.0 12.0 22.0 5 22.46691058925526 22.0 22.0 22.0 22.0 27.0 6 26.813615180915157 27.0 22.0 32.0 22.0 32.0 7 35.0065100890754 37.0 37.0 37.0 27.0 41.0 8 37.63418206184491 41.0 37.0 41.0 32.0 41.0 9 38.58759195149218 41.0 41.0 41.0 32.0 41.0 10-11 38.946945118042635 41.0 41.0 41.0 37.0 41.0 12-13 39.13194275372079 41.0 41.0 41.0 37.0 41.0 14-15 39.08788917159351 41.0 41.0 41.0 37.0 41.0 16-17 38.99003687591983 41.0 41.0 41.0 37.0 41.0 18-19 38.92199941306062 41.0 41.0 41.0 37.0 41.0 20-21 38.92933740544666 41.0 41.0 41.0 37.0 41.0 22-23 38.923911341525276 41.0 41.0 41.0 37.0 41.0 24-25 38.94057254405092 41.0 41.0 41.0 37.0 41.0 26-27 38.89994871312447 41.0 41.0 41.0 37.0 41.0 28-29 38.83997744752628 41.0 41.0 41.0 37.0 41.0 30-31 38.72730555757835 41.0 41.0 41.0 32.0 41.0 32-33 38.784553243183865 41.0 41.0 41.0 32.0 41.0 34-35 38.750688903689124 41.0 41.0 41.0 32.0 41.0 36-37 38.67398299000922 41.0 41.0 41.0 32.0 41.0 38-39 38.57290502802026 41.0 41.0 41.0 32.0 41.0 40-41 38.62298677136148 41.0 41.0 41.0 32.0 41.0 42-43 38.36751265529438 41.0 41.0 41.0 32.0 41.0 44-45 38.47798133482766 41.0 41.0 41.0 32.0 41.0 46-47 38.487594420513005 41.0 41.0 41.0 32.0 41.0 48-49 38.42857357166364 41.0 41.0 41.0 32.0 41.0 50-51 38.30559367605635 41.0 41.0 41.0 32.0 41.0 52-53 38.26628327044622 41.0 41.0 41.0 32.0 41.0 54-55 38.16104375823723 41.0 39.0 41.0 32.0 41.0 56-57 37.95664290051815 41.0 37.0 41.0 29.5 41.0 58-59 37.94501646899394 41.0 37.0 41.0 29.5 41.0 60-61 37.94536447591595 41.0 37.0 41.0 32.0 41.0 62-63 37.93216990363631 41.0 37.0 41.0 32.0 41.0 64-65 37.909424283726125 41.0 37.0 41.0 32.0 41.0 66-67 37.78283180436354 41.0 37.0 41.0 27.0 41.0 68-69 37.810961452334304 41.0 37.0 41.0 27.0 41.0 70-71 37.77841569497154 41.0 37.0 41.0 27.0 41.0 72-73 37.63836861480743 41.0 37.0 41.0 27.0 41.0 74-75 37.55440034203752 41.0 37.0 41.0 27.0 41.0 76-77 36.44816572067749 39.0 34.5 41.0 24.5 41.0 78-79 37.00380916781809 41.0 37.0 41.0 27.0 41.0 80-81 37.68564786325 41.0 37.0 41.0 27.0 41.0 82-83 37.876521143412916 41.0 37.0 41.0 32.0 41.0 84-85 37.8097152218884 41.0 37.0 41.0 27.0 41.0 86-87 37.67781247255559 41.0 37.0 41.0 27.0 41.0 88-89 37.59445004748959 41.0 37.0 41.0 27.0 41.0 90-91 37.43678728510534 41.0 37.0 41.0 27.0 41.0 92-93 37.303174035652034 41.0 37.0 41.0 27.0 41.0 94-95 37.13449975294165 41.0 37.0 41.0 27.0 41.0 96-97 37.068214513529455 41.0 37.0 41.0 27.0 41.0 98-99 36.934715339088754 41.0 37.0 41.0 27.0 41.0 100 36.79749372509297 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4697353084420772 1101 2 -0.5095949147047065 1101 3 -0.013613631053464914 1101 4 0.07185669525639504 1101 5 -1.1540221386280933 1101 6 -0.6746064796285367 1101 7 -0.4827793157615474 1101 8 -0.1782376668017136 1101 9 -0.16379589613423917 1101 10-11 -0.18435110996097848 1101 12-13 -0.04919994665259253 1101 14-15 -0.1068649919693172 1101 16-17 -0.11689910949093019 1101 18-19 -0.1147488640016121 1101 20-21 0.0692079618517667 1101 22-23 0.03642294152374603 1101 24-25 -0.053125531981045526 1101 26-27 0.04990850608032105 1101 28-29 0.023468319801771997 1101 30-31 0.10608483619817832 1101 32-33 -0.007895613932880963 1101 34-35 0.06140700648793285 1101 36-37 0.08469626309825884 1101 38-39 0.03416938084843224 1101 40-41 0.09286655550786094 1101 42-43 0.08503094694686553 1101 44-45 0.010313132171376083 1101 46-47 0.04148813668575002 1101 48-49 0.13249165840564103 1101 50-51 -0.029427285323102126 1101 52-53 0.032675749403544785 1101 54-55 0.12690767220783528 1101 56-57 0.312462863037851 1101 58-59 0.1764224780130732 1101 60-61 0.0765277417080128 1101 62-63 -0.1732912517961367 1101 64-65 -0.13807558630589511 1101 66-67 0.023920103124694947 1101 68-69 0.0711248180128834 1101 70-71 0.017764674752591247 1101 72-73 0.2578763447605752 1101 74-75 0.06798849688323827 1101 76-77 -0.3077175960432328 1101 78-79 -0.04849663677777727 1101 80-81 -0.008944263674287356 1101 82-83 -0.06399661193299977 1101 84-85 -0.016675849996751424 1101 86-87 -0.07856796727050153 1101 88-89 -0.3502750183179302 1101 90-91 -0.18791734774117685 1101 92-93 0.13148480967127796 1101 94-95 0.15624003575496204 1101 96-97 -0.0693431669375073 1101 98-99 -0.11280785300752427 1101 100 -0.3749661526278629 1102 1 1.6877967154354536 1102 2 0.7227424464071817 1102 3 -0.04654828953153789 1102 4 0.038868573442048415 1102 5 -0.7499344314032683 1102 6 -5.583586158408593E-5 1102 7 -0.17824606001222065 1102 8 -0.47218070596857586 1102 9 -0.31495887911486875 1102 10-11 -0.2321212225883329 1102 12-13 -0.2462967631595845 1102 14-15 -0.22246640166073206 1102 16-17 -0.4217060229219811 1102 18-19 -0.33949517736266444 1102 20-21 -0.27199417991435126 1102 22-23 -0.1586487957051972 1102 24-25 -0.2100735587798468 1102 26-27 -0.12207098637855296 1102 28-29 -0.2868016140964116 1102 30-31 -0.2550917053332 1102 32-33 -0.26945572381868743 1102 34-35 -0.15717803036758227 1102 36-37 -0.26638633837794856 1102 38-39 -0.3168046776218105 1102 40-41 -0.1790444914472502 1102 42-43 -0.3826850931108581 1102 44-45 -0.09330863586115612 1102 46-47 -0.6079841615352279 1102 48-49 -0.4940959695566747 1102 50-51 -0.25679025599473704 1102 52-53 -0.23842010600673547 1102 54-55 -0.2879876484447479 1102 56-57 -0.07563725514098962 1102 58-59 -0.1796738032388916 1102 60-61 -0.2061763095073772 1102 62-63 -0.2975457646301365 1102 64-65 -0.22480424557596734 1102 66-67 -0.13790726127581365 1102 68-69 -0.26787388829871617 1102 70-71 -0.40208321583904194 1102 72-73 -0.3363281052540046 1102 74-75 -0.4662806313348966 1102 76-77 -0.31997356855064396 1102 78-79 -0.5109174442109179 1102 80-81 -0.25351011962541037 1102 82-83 -0.37722275749376877 1102 84-85 -0.40355283894164984 1102 86-87 -0.2966498270983351 1102 88-89 -0.2536960501727492 1102 90-91 -0.369798063577953 1102 92-93 -0.28458260137909264 1102 94-95 -0.23892905447933543 1102 96-97 -0.21051938520219693 1102 98-99 -0.3973180324889114 1102 100 -0.4577604698482105 1103 1 1.3650785281096205 1103 2 0.6672337801420056 1103 3 0.6261335807658188 1103 4 0.9736040024091075 1103 5 2.8424183172718216 1103 6 -0.19705299306604118 1103 7 -0.7152851806998441 1103 8 -0.48413777540451974 1103 9 -0.5059452526980053 1103 10-11 -0.39074089743184715 1103 12-13 -0.4717938404176607 1103 14-15 -0.39425791900188756 1103 16-17 -0.4721749129649311 1103 18-19 -0.39566317599700795 1103 20-21 -0.2819468609246769 1103 22-23 -0.20844625199409705 1103 24-25 -0.3126451545637323 1103 26-27 -0.43021118890424503 1103 28-29 -0.3051706795579463 1103 30-31 -0.40107161239971134 1103 32-33 -0.44636071996555415 1103 34-35 -0.5770704027101985 1103 36-37 -0.35877159343265674 1103 38-39 -0.3517509667836336 1103 40-41 -0.44786954739040397 1103 42-43 -0.28269926717974414 1103 44-45 -0.5984212134999041 1103 46-47 -0.5879617765479495 1103 48-49 -0.6610578756844347 1103 50-51 -0.45249365929037566 1103 52-53 -0.2308199857138149 1103 54-55 -0.43917552810960103 1103 56-57 -0.40952701615923104 1103 58-59 -0.6229238438557729 1103 60-61 -0.4256553938084551 1103 62-63 -0.3770028784807309 1103 64-65 -0.5322916170084113 1103 66-67 -0.7067938154270621 1103 68-69 -0.5116518453944181 1103 70-71 -0.6120707591368983 1103 72-73 -0.48490517549842593 1103 74-75 -0.545874336348966 1103 76-77 -0.6926319827594227 1103 78-79 -0.5537706366463766 1103 80-81 -0.5844860216484804 1103 82-83 -0.6165185676081109 1103 84-85 -0.5924078239269193 1103 86-87 -0.5973355121641646 1103 88-89 -0.46878664448720997 1103 90-91 -0.7914468746941239 1103 92-93 -0.45878641393327513 1103 94-95 -0.5362934243460629 1103 96-97 -0.3747408426054477 1103 98-99 -0.4693506248656121 1103 100 -0.4903632743312869 1104 1 1.555870175660898 1104 2 -0.4943085388090509 1104 3 -0.05059069211837652 1104 4 0.15564125406752005 1104 5 -1.1056271518696619 1104 6 -0.4918934290444277 1104 7 -0.34100286495215215 1104 8 -0.256346624400571 1104 9 -0.1577398223487947 1104 10-11 -0.2553242388512089 1104 12-13 -0.17265654935113162 1104 14-15 -0.16663573381586616 1104 16-17 -0.18228229035409527 1104 18-19 -0.17208795502827456 1104 20-21 -0.15149949222443837 1104 22-23 -0.013342871047591132 1104 24-25 -0.1170400512267733 1104 26-27 -0.2816767150904482 1104 28-29 -0.25658310959433095 1104 30-31 -0.11515246810678548 1104 32-33 -0.22866210685665322 1104 34-35 -0.3495903377010521 1104 36-37 -0.23282834110717232 1104 38-39 -0.21953928961546865 1104 40-41 -0.1345617685198306 1104 42-43 -0.2102356368779894 1104 44-45 -0.23362957578125076 1104 46-47 -0.2786261175411511 1104 48-49 -0.3624518048693801 1104 50-51 -0.21551567116352288 1104 52-53 -0.16318197259658263 1104 54-55 -0.31080109571701797 1104 56-57 -0.26148676089405853 1104 58-59 -0.26691053669791387 1104 60-61 -0.29127659417991936 1104 62-63 -0.43469390235924976 1104 64-65 -0.6144145030273833 1104 66-67 -0.8453234686693563 1104 68-69 -0.4830955224632163 1104 70-71 -0.4603965743969667 1104 72-73 -0.2807180058777021 1104 74-75 -0.48644681996794503 1104 76-77 -0.49488686135631355 1104 78-79 -0.28660255809305113 1104 80-81 -0.45439236852728726 1104 82-83 -0.45994579729061513 1104 84-85 -0.4070690112365227 1104 86-87 -0.33531985604506076 1104 88-89 -0.4310285414377262 1104 90-91 -0.44297454045334206 1104 92-93 -0.4560817964571413 1104 94-95 -0.3391307563214596 1104 96-97 -0.3748332174859996 1104 98-99 -0.42243156000990467 1104 100 -0.34403279530413755 1105 1 1.378691302763924 1105 2 0.28917481902437636 1105 3 -0.06592145571901398 1105 4 -0.19566169421321433 1105 5 -1.1646686736648704 1105 6 0.4061437246989037 1105 7 0.009490654557204437 1105 8 -0.2193655542710644 1105 9 -0.15852209736450362 1105 10-11 -0.20705726559395998 1105 12-13 -0.19479722802222454 1105 14-15 -0.21429390752831523 1105 16-17 -0.1872479717097164 1105 18-19 -0.1327253771000727 1105 20-21 -0.13195347833406146 1105 22-23 -0.20907325153341816 1105 24-25 -0.1764102568917778 1105 26-27 -0.1568342994429699 1105 28-29 -0.13955486598564448 1105 30-31 -0.2104699880802201 1105 32-33 -0.18108570297663107 1105 34-35 -0.12463850476083138 1105 36-37 -0.2428728856090956 1105 38-39 -0.274797367549219 1105 40-41 -0.19727410780353694 1105 42-43 0.10439258500768744 1105 44-45 -0.10695576273980123 1105 46-47 -0.3015174124173612 1105 48-49 -0.20261609743381825 1105 50-51 -0.06035170567841419 1105 52-53 -0.15355473194889413 1105 54-55 -0.22649491737362126 1105 56-57 -0.0695756795697946 1105 58-59 -0.24824307022971226 1105 60-61 -0.26477866341953415 1105 62-63 -0.4074117834776416 1105 64-65 -0.3618076807696937 1105 66-67 -0.37331825021122 1105 68-69 -0.46334844735469716 1105 70-71 -0.39308833853944236 1105 72-73 -0.23298269081391254 1105 74-75 -0.40844932305051174 1105 76-77 -0.4762640751588165 1105 78-79 -0.36070466361994846 1105 80-81 -0.502091766577891 1105 82-83 -0.44113774842372777 1105 84-85 -0.48461149760719735 1105 86-87 -0.37193700413547504 1105 88-89 -0.24360103887971718 1105 90-91 -0.3149810548861822 1105 92-93 -0.28121790465744567 1105 94-95 -0.17369285881208185 1105 96-97 -0.18495163254597458 1105 98-99 -0.3882326390014157 1105 100 -0.5180082655886693 1106 1 1.215278160299592 1106 2 -0.8693014775269798 1106 3 -0.12957152700898256 1106 4 -0.28404882547252974 1106 5 -1.1566440459949163 1106 6 -0.7128522241413755 1106 7 -0.16503887515275295 1106 8 -0.172649927286038 1106 9 -0.26383509575721575 1106 10-11 -0.14390125181579094 1106 12-13 -0.10070950966174053 1106 14-15 -0.248542056288791 1106 16-17 -0.17708626094933777 1106 18-19 -0.13715890464212066 1106 20-21 -0.23801729000423677 1106 22-23 -0.20556910403590223 1106 24-25 -0.34132216685386396 1106 26-27 -0.16325841447154943 1106 28-29 -0.2664807206883566 1106 30-31 -0.30846187333009567 1106 32-33 -0.3185497316865309 1106 34-35 -0.2441541804741334 1106 36-37 -0.502231512068299 1106 38-39 -0.29818364730672187 1106 40-41 -0.4467031043614824 1106 42-43 -0.20962959878990972 1106 44-45 -0.2208323210813461 1106 46-47 -0.4566044209858333 1106 48-49 -0.3098680959769382 1106 50-51 -0.27953075848520115 1106 52-53 -0.42698066558644854 1106 54-55 -0.4462537994076783 1106 56-57 -0.4605872388622885 1106 58-59 -0.3623137336925808 1106 60-61 -0.11844111567016569 1106 62-63 -0.3162948741433169 1106 64-65 -0.6832816863460991 1106 66-67 -0.640843462744833 1106 68-69 -0.4697851795269159 1106 70-71 -0.839625460910824 1106 72-73 -0.5174970426594285 1106 74-75 -0.6276789065853237 1106 76-77 -0.548772500054497 1106 78-79 -0.4547737359553423 1106 80-81 -0.38859701667702495 1106 82-83 -0.4391442397266019 1106 84-85 -0.7066079648069703 1106 86-87 -0.5961663328412783 1106 88-89 -0.698977309450953 1106 90-91 -0.7803435149681519 1106 92-93 -0.6199843582207691 1106 94-95 -0.3913820320597594 1106 96-97 -0.5490895460060372 1106 98-99 -0.6438509971318922 1106 100 -0.7095363819299223 1107 1 1.3708645827056785 1107 2 -0.5698228754301802 1107 3 -0.11095759904419822 1107 4 -0.37673368616980873 1107 5 -1.2640671516581783 1107 6 -0.3330470808552093 1107 7 -0.49027359042000285 1107 8 -0.5198786681442016 1107 9 -0.28074027477016017 1107 10-11 -0.2808329862935963 1107 12-13 -0.41426088534537087 1107 14-15 -0.4741028766201367 1107 16-17 -0.2877446174011169 1107 18-19 -0.31682578100389946 1107 20-21 -0.3323911852697634 1107 22-23 -0.28193368802690344 1107 24-25 -0.4141787315914556 1107 26-27 -0.49877269110834277 1107 28-29 -0.2682069019729525 1107 30-31 -0.43641430829101324 1107 32-33 -0.23151560919466618 1107 34-35 -0.3337350083058226 1107 36-37 -0.4337919834994395 1107 38-39 -0.4307415144107267 1107 40-41 -0.5180708211011549 1107 42-43 -0.3799444406653407 1107 44-45 -0.2927298539561889 1107 46-47 -0.4391246722852671 1107 48-49 -0.4059392031595479 1107 50-51 -0.31743302382635363 1107 52-53 -0.4206686397617503 1107 54-55 -0.402815405525196 1107 56-57 -0.24202527826621179 1107 58-59 -0.43513613105798044 1107 60-61 -0.4597801368884262 1107 62-63 -0.45870150213175975 1107 64-65 -0.44412865429925574 1107 66-67 -0.4393547922397545 1107 68-69 -0.7033019897472599 1107 70-71 -0.5517488686415533 1107 72-73 -0.3867721061067826 1107 74-75 -0.5232247490563751 1107 76-77 -0.6642076326327739 1107 78-79 -0.8324972685395977 1107 80-81 -0.6009840646693121 1107 82-83 -0.5083686670015766 1107 84-85 -0.49820208127422205 1107 86-87 -0.38468684378738516 1107 88-89 -0.33950479535864986 1107 90-91 -0.36712711899075146 1107 92-93 -0.3565130318091505 1107 94-95 -0.23670792286463893 1107 96-97 -0.3800475813732618 1107 98-99 -0.6121392384085595 1107 100 -0.6140142981436654 1108 1 1.555365753815563 1108 2 -0.4975777991900898 1108 3 0.06371964185439793 1108 4 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0.3326584393485632 2223 28-29 0.2809990133610256 2223 30-31 0.24036039750668436 2223 32-33 0.3946241039289262 2223 34-35 0.3375094211413696 2223 36-37 0.3777579281133896 2223 38-39 0.34004518787603644 2223 40-41 0.263860547821821 2223 42-43 0.5380865908686658 2223 44-45 0.511432275469744 2223 46-47 0.38925563960183496 2223 48-49 0.28286882993001683 2223 50-51 0.3172907312751292 2223 52-53 0.2853817676935293 2223 54-55 0.2563265635709726 2223 56-57 0.5996325103894833 2223 58-59 0.4717811779444503 2223 60-61 0.5696424264720861 2223 62-63 0.4170520236542572 2223 64-65 0.3796501955525784 2223 66-67 0.5523151039108498 2223 68-69 0.6392622647036532 2223 70-71 0.5822257198632599 2223 72-73 0.6749939230622033 2223 74-75 0.5361865811029745 2223 76-77 0.464580011730078 2223 78-79 0.6551346675446155 2223 80-81 0.5337878257060353 2223 82-83 0.4160082390693134 2223 84-85 0.45816329296034297 2223 86-87 0.5821618282439118 2223 88-89 0.5673437489668132 2223 90-91 0.5368166089093833 2223 92-93 0.4533383922827525 2223 94-95 0.6111315646616973 2223 96-97 0.5674918465733896 2223 98-99 0.4510022070937154 2223 100 0.49539300437032097 2224 1 1.1766007104294296 2224 2 -0.926853055540084 2224 3 -0.025851769307863037 2224 4 -0.22527841895189127 2224 5 -0.9782575238390869 2224 6 0.5702552229567424 2224 7 0.5074000622227075 2224 8 0.26913618788921667 2224 9 0.41708765873305964 2224 10-11 0.41140963336074066 2224 12-13 0.3015442353924058 2224 14-15 0.3708977301023424 2224 16-17 0.3500342582554481 2224 18-19 0.2733721838755372 2224 20-21 0.26630436138719205 2224 22-23 0.314445912229651 2224 24-25 0.37498347133121257 2224 26-27 0.4015280698950008 2224 28-29 0.31967858349170797 2224 30-31 0.33256137080479675 2224 32-33 0.37936725869585075 2224 34-35 0.3683928541241883 2224 36-37 0.38169157432121636 2224 38-39 0.21370437461106206 2224 40-41 0.21997327895604712 2224 42-43 0.5310476372724935 2224 44-45 0.579106955030241 2224 46-47 0.5233954795687055 2224 48-49 0.36794139385537505 2224 50-51 0.39574585529719286 2224 52-53 0.4138668708385609 2224 54-55 0.42158870631975986 2224 56-57 0.520668222869233 2224 58-59 0.5216175569479873 2224 60-61 0.43539555260834106 2224 62-63 0.5789993663481425 2224 64-65 0.4766940613905817 2224 66-67 0.6134413316703089 2224 68-69 0.6298571275582034 2224 70-71 0.7853088740041088 2224 72-73 0.6238117342731968 2224 74-75 0.6027005027334624 2224 76-77 0.7937707680467554 2224 78-79 0.6741385419549601 2224 80-81 0.46027757140119974 2224 82-83 0.49039535262156164 2224 84-85 0.5340296173753885 2224 86-87 0.5632546334951911 2224 88-89 0.373820563907735 2224 90-91 0.40662173257025813 2224 92-93 0.47557652042965515 2224 94-95 0.6426834478081318 2224 96-97 0.7120866949801581 2224 98-99 0.47640150734005005 2224 100 0.7440126403346738 2225 1 1.537424001476074 2225 2 1.6061866291385325 2225 3 0.8514462459769465 2225 4 0.9750291060254899 2225 5 3.1821668028785304 2225 6 -0.13667299233961927 2225 7 0.28977103648493596 2225 8 0.42260823632319955 2225 9 0.32587738672505395 2225 10-11 0.17563639332762904 2225 12-13 0.2717627995058294 2225 14-15 0.19344005689696075 2225 16-17 0.15779529354141175 2225 18-19 0.17773684882249796 2225 20-21 0.2626784301495846 2225 22-23 0.28710843364570593 2225 24-25 0.3167890616444211 2225 26-27 0.19541005538021494 2225 28-29 0.23101598026148906 2225 30-31 0.27750597780809017 2225 32-33 0.16142276719489246 2225 34-35 0.22177117722272754 2225 36-37 0.29122220169141855 2225 38-39 0.07853788280210239 2225 40-41 0.20472600769880245 2225 42-43 0.4994759151326278 2225 44-45 0.4772550675295122 2225 46-47 0.3549847676892881 2225 48-49 0.22004774744654299 2225 50-51 0.28026649652768043 2225 52-53 0.28569103777794425 2225 54-55 0.31560941492661243 2225 56-57 0.40518693906073366 2225 58-59 0.4808157541489848 2225 60-61 0.4784761203023322 2225 62-63 0.42130245892326457 2225 64-65 0.5051389186489459 2225 66-67 0.5643451374723796 2225 68-69 0.533814887390335 2225 70-71 0.5811078373706167 2225 72-73 0.6172963195235468 2225 74-75 0.5494262520548787 2225 76-77 0.7369160651765227 2225 78-79 0.5744043176494245 2225 80-81 0.3577108928634587 2225 82-83 0.3938802747537409 2225 84-85 0.302424447683201 2225 86-87 0.3107461873866555 2225 88-89 0.4639802458912925 2225 90-91 0.6504405527138672 2225 92-93 0.5063332291905098 2225 94-95 0.7072971406104145 2225 96-97 0.5444287936576586 2225 98-99 0.6375412266693701 2225 100 0.7272202846644689 2226 1 1.127535612281985 2226 2 1.6680946796515546 2226 3 0.8269184380264587 2226 4 0.8418551236706637 2226 5 3.1211232330519323 2226 6 -0.37968095533007684 2226 7 0.06553646819779857 2226 8 0.07749338249369231 2226 9 0.2538041178354149 2226 10-11 0.1665446939051236 2226 12-13 0.10110869532272204 2226 14-15 0.1513967469029751 2226 16-17 0.13312926636252342 2226 18-19 0.07058300251836869 2226 20-21 0.11003775911721192 2226 22-23 0.16751021691795387 2226 24-25 0.1387646491005583 2226 26-27 0.09081033435838748 2226 28-29 0.198707113522417 2226 30-31 0.10672220152201817 2226 32-33 0.046174793107823575 2226 34-35 0.19955768663531614 2226 36-37 0.18664983147097303 2226 38-39 0.17251694576364685 2226 40-41 0.2410068457273553 2226 42-43 0.31193562154130916 2226 44-45 0.12184507901685038 2226 46-47 0.17221132805250505 2226 48-49 0.15740391153571665 2226 50-51 0.10612809561181535 2226 52-53 0.2558356844446621 2226 54-55 0.25860961224310586 2226 56-57 0.3094183522375715 2226 58-59 0.3816410157398593 2226 60-61 0.4292893670768976 2226 62-63 0.3642919112820735 2226 64-65 0.260058155639733 2226 66-67 0.3726169966235702 2226 68-69 0.31868056565113534 2226 70-71 0.3227963683981443 2226 72-73 0.27066032157095066 2226 74-75 0.4984253104058638 2226 76-77 0.5372066186004503 2226 78-79 0.4957421918669951 2226 80-81 0.32863235113004663 2226 82-83 0.21117277008456625 2226 84-85 0.3089942566964581 2226 86-87 0.22188953879756923 2226 88-89 0.2198342942006235 2226 90-91 0.31481764798061107 2226 92-93 0.12638981604492727 2226 94-95 0.3965446614556285 2226 96-97 0.24591824015558927 2226 98-99 0.3022536134565854 2226 100 0.551521178644947 2227 1 1.5116782169361116 2227 2 1.7117317185225627 2227 3 0.6117275639006703 2227 4 -0.24345787655731144 2227 5 3.3190872668903246 2227 6 0.17652156514479955 2227 7 0.31653477921979345 2227 8 0.21624758348943374 2227 9 0.24686803180294703 2227 10-11 0.2324269704219546 2227 12-13 0.2854303095836741 2227 14-15 0.197457371162983 2227 16-17 0.24291706551876757 2227 18-19 0.15123648257298328 2227 20-21 0.11348459798282562 2227 22-23 0.11182572732514728 2227 24-25 0.3463410882250386 2227 26-27 0.32906084485137654 2227 28-29 0.17237817708829084 2227 30-31 0.2846130136287286 2227 32-33 0.24300532414309828 2227 34-35 0.33518733959812863 2227 36-37 0.3326915803598993 2227 38-39 0.2676754130835164 2227 40-41 0.38132721225274224 2227 42-43 0.5279217125990385 2227 44-45 0.37638803764546935 2227 46-47 0.30099498198115526 2227 48-49 0.26655491205295334 2227 50-51 0.3696303594306727 2227 52-53 0.35382876674373165 2227 54-55 0.5432159504456351 2227 56-57 0.4162753823327563 2227 58-59 0.4947487352763602 2227 60-61 0.4369142815861693 2227 62-63 0.4966389656210808 2227 64-65 0.48784116976684544 2227 66-67 0.31175170794069373 2227 68-69 0.42416355452937893 2227 70-71 0.3809495481627678 2227 72-73 0.4447218846968113 2227 74-75 0.40471712119129677 2227 76-77 0.6382945876350377 2227 78-79 0.518506563500992 2227 80-81 0.24535960692073644 2227 82-83 0.35157899503487045 2227 84-85 0.2276047597373534 2227 86-87 0.21122201252525485 2227 88-89 0.377407240155776 2227 90-91 0.43673091197351965 2227 92-93 0.41655200941207937 2227 94-95 0.5419011363207389 2227 96-97 0.5036470304894323 2227 98-99 0.5962443204005154 2227 100 0.6669101215996562 2228 1 1.3365539313160628 2228 2 1.4484950751038532 2228 3 -0.16395263375694213 2228 4 -0.05051382157779116 2228 5 3.258923431756923 2228 6 0.15402841659574662 2228 7 0.47549863594201014 2228 8 0.4597550466235276 2228 9 0.3928276247338047 2228 10-11 0.3629136783072724 2228 12-13 0.3392080527127348 2228 14-15 0.3089021427712879 2228 16-17 0.26674332351713304 2228 18-19 0.22758833231482356 2228 20-21 0.20995602578322092 2228 22-23 0.3570944781122378 2228 24-25 0.4347835750168727 2228 26-27 0.34578738050590374 2228 28-29 0.44293732744840497 2228 30-31 0.39411569124172274 2228 32-33 0.3211646376187218 2228 34-35 0.32482980480116197 2228 36-37 0.4658945812054611 2228 38-39 0.25514910171281 2228 40-41 0.26109489259432195 2228 42-43 0.5808661873611172 2228 44-45 0.40830230605443774 2228 46-47 0.32870093029992375 2228 48-49 0.2760728817446534 2228 50-51 0.4567178481115306 2228 52-53 0.3913363378219188 2228 54-55 0.30412370023124424 2228 56-57 0.5199538768725773 2228 58-59 0.5773769338694876 2228 60-61 0.428132130740309 2228 62-63 0.3438276218971339 2228 64-65 0.35410324601134846 2228 66-67 0.46073103664843273 2228 68-69 0.4206640672637789 2228 70-71 0.5623207978554277 2228 72-73 0.47817195755906994 2228 74-75 0.5008287353751513 2228 76-77 0.44247951984015543 2228 78-79 0.49980255217884917 2228 80-81 0.4681606959566196 2228 82-83 0.3302522422927865 2228 84-85 0.4317063351213051 2228 86-87 0.5562239015467441 2228 88-89 0.4760570395596915 2228 90-91 0.4334913900458801 2228 92-93 0.5244643108399387 2228 94-95 0.743141593763788 2228 96-97 0.5102535083339106 2228 98-99 0.6844160474459073 2228 100 0.6982498876008663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 21.0 13 41.0 14 179.0 15 520.0 16 1880.0 17 4692.0 18 7737.0 19 10240.0 20 11941.0 21 14258.0 22 16421.0 23 18907.0 24 21257.0 25 24542.0 26 27781.0 27 31811.0 28 35727.0 29 40915.0 30 47050.0 31 54626.0 32 64282.0 33 77734.0 34 94640.0 35 122917.0 36 173665.0 37 287244.0 38 709220.0 39 1298015.0 40 1386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.143963483506518 19.344075165089915 19.882670881988386 37.62929046941518 2 23.009442855285396 47.10263244751608 14.497370803036516 15.390553894162007 3 37.03993781817943 15.983128149368882 24.913576457917728 22.063357574533956 4 20.03279109677343 17.37778112536719 20.453524745995576 42.135903031863805 5 16.34848072397941 35.30827912686673 26.519346340801757 21.823893808352103 6 18.015507325959817 28.945799992436672 29.230299948525605 23.80839273307791 7 20.217405096621537 26.074516298506495 35.65622354201977 18.051855062852205 8 21.849990420824284 28.05438971587196 32.66523921842677 17.430380644876987 9 21.449071445024124 28.250504977264697 26.782062657497747 23.518360920213436 10-11 19.97079992211646 29.942971869726957 28.452511509856237 21.633716698300347 12-13 24.348092556402282 26.74735572558115 27.083298824339796 21.821252893676775 14-15 22.053434611108905 27.402208804778272 28.351891098054814 22.192465486058 16-17 20.757370574084845 29.53705234542914 28.365486339282842 21.340090741203177 18-19 21.415755290658446 29.510471304821245 29.553397888330878 19.520375516189432 20-21 22.978661096889063 28.728448026642923 29.685365488527022 18.60752538794099 22-23 21.648749597221446 30.193358709033397 28.67687987026077 19.481011823484387 24-25 22.273005570298494 28.50834575917546 26.396661008754396 22.82198766177165 26-27 20.32504190303374 26.712023725102345 29.763514685517066 23.199419686346843 28-29 19.807237749485022 27.789602020976726 30.518845708002868 21.884314521535384 30-31 20.25040328894993 29.934386036528398 28.120312478999438 21.694898195522235 32-33 21.170884681413494 29.780532177123177 28.474170135536742 20.574413005926587 34-35 21.483325202233118 28.835537898063198 29.912593537603655 19.76854336210003 36-37 21.981254818887948 28.056546202411575 28.66776949596659 21.294429482733886 38-39 21.56899207749918 29.279374025861188 28.880436151656713 20.27119774498292 40-41 21.048830939987663 29.562221908641405 29.708271392006598 19.680675759364334 42-43 20.82806061297425 29.642429081976278 29.05495765936534 20.47455264568413 44-45 20.62657497744284 30.129867369827128 28.517346746471254 20.72621090625878 46-47 20.266550818037693 28.037670598696234 28.74915516189247 22.9466234213736 48-49 21.166337307623433 28.23026838256321 29.87812100639789 20.725273303415467 50-51 22.107534295168 30.68108408141018 27.916249563624014 19.295132059797808 52-53 20.35697202140326 30.19869787112884 28.172495586566658 21.27183452090124 54-55 22.349537597821858 28.43589747597337 27.9966896474115 21.21787527879327 56-57 21.34301388411783 28.710576516433296 29.21459821890452 20.731811380544357 58-59 21.22417077099734 27.914736408274482 28.958369247691934 21.902723573036248 60-61 19.844469978571023 30.11814299704701 29.38627929802717 20.651107726354798 62-63 20.41755541274493 30.994316192724327 27.303373525910963 21.284754868619782 64-65 19.989308182194076 30.228434859764043 28.58273563644444 21.19952132159744 66-67 20.2913975876729 30.175137960445035 29.234269133895623 20.29919531798644 68-69 21.074545989263196 29.793564856645215 29.05640587451936 20.07548327957223 70-71 19.686815647591345 30.588136386209865 29.25308369761808 20.471964268580713 72-73 20.203372307399967 30.582901437001368 28.430618483464905 20.783107772133754 74-75 20.150788414604225 29.24723930656144 30.06975140085678 20.532220877977554 76-77 19.692956946215038 30.32446683995651 28.70980535677507 21.272770857053384 78-79 19.803219040588516 29.37072160102561 28.367720959392734 22.45833839899314 80-81 21.312034898920675 29.190482493267606 28.084389480630715 21.413093127181007 82-83 20.580757451832998 28.102832529443074 29.58251045661571 21.73389956210822 84-85 20.695535666568425 28.734417431412005 28.580963099389965 21.9890838026296 86-87 21.329683349642416 27.737917502826093 29.658940715059686 21.273458432471813 88-89 20.843083100677607 27.168026867947077 29.37840994434077 22.610480087034546 90-91 21.114097202536904 27.065140582607654 28.79359579753904 23.0271664173164 92-93 21.397378274929533 27.083611358620896 29.348062865645574 22.170947500803994 94-95 21.858944527977915 27.172324214312262 28.544490348785136 22.4242409089247 96-97 21.112878318840597 27.464262486291464 28.925000836029202 22.497858358838734 98-99 22.718554441440293 28.024589572168697 28.023980130320545 21.232875856070464 100 21.60677624326918 29.651314878600747 26.99736752375026 21.74454135437981 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 8.0 2 15.0 3 20.0 4 25.5 5 24.5 6 33.5 7 50.5 8 59.5 9 75.5 10 83.0 11 97.5 12 120.5 13 134.0 14 146.0 15 180.0 16 255.0 17 338.5 18 484.0 19 789.5 20 1263.5 21 1949.5 22 3014.0 23 4634.0 24 7268.5 25 11055.0 26 15797.5 27 21484.0 28 27875.5 29 35247.0 30 43627.5 31 53062.0 32 64077.5 33 76351.0 34 89470.5 35 103153.5 36 117262.5 37 128957.0 38 137267.0 39 144070.0 40 149132.0 41 152072.0 42 151595.0 43 149922.5 44 149888.0 45 147216.0 46 138184.0 47 127137.5 48 120120.0 49 117946.0 50 114166.5 51 105203.0 52 92699.0 53 78921.5 54 66772.5 55 55610.5 56 44190.0 57 32990.5 58 23346.0 59 16552.0 60 12645.0 61 10413.5 62 8517.0 63 6537.5 64 4954.0 65 4268.0 66 4756.0 67 4647.0 68 3252.5 69 2898.5 70 3900.5 71 4001.5 72 2491.0 73 1002.5 74 375.0 75 200.0 76 166.5 77 132.5 78 112.5 79 102.5 80 70.5 81 46.0 82 42.5 83 38.0 84 30.0 85 25.0 86 20.5 87 23.5 88 23.0 89 21.5 90 21.5 91 20.0 92 17.0 93 15.0 94 18.5 95 17.5 96 16.0 97 19.0 98 51.5 99 111.0 100 103.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.345400698639132 2 0.006031911625306401 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.500274248831669E-4 30-31 0.010313631276430634 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.750959870910841E-4 40-41 0.0015157912633541992 42-43 1.2501371244158345E-4 44-45 0.0 46-47 0.002234620109893304 48-49 0.0 50-51 0.0 52-53 0.0013282706946918241 54-55 0.01856453629757514 56-57 0.011938809538171218 58-59 0.015236046203817981 60-61 2.9690756704876066E-4 62-63 0.012032569822502406 64-65 1.8752056866237516E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.656541389383648E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3199649.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.03826875433303 #Duplication Level Percentage of deduplicated Percentage of total 1 86.55743974502026 64.95120426273817 2 8.51417085848391 12.777772821984518 3 2.2832486607728923 5.139930799201418 4 0.9448530781666464 2.836005568513106 5 0.47741585116260726 1.7912229473559187 6 0.2953108791112062 1.3295770267695022 7 0.19511316973077097 1.024866812743717 8 0.139092256984214 0.8349793728982565 9 0.103896506378817 0.7016592571460952 >10 0.4557950991854187 5.8651612202572245 >50 0.023548136042750207 1.2056489694895747 >100 0.009608976022734646 1.2661720426012446 >500 5.06782937908381E-4 0.27579889830120047 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.125342811039586E-5 0.0 0.0 0.0 0.0 2 3.125342811039586E-5 0.0 0.0 0.0 0.0 3 3.125342811039586E-5 0.0 0.0 0.0 0.0 4 3.125342811039586E-5 0.0 0.0 0.0 0.0 5 3.125342811039586E-5 0.0 0.0 0.0 0.0 6 3.125342811039586E-5 0.0 0.0 0.0 0.0 7 3.125342811039586E-5 0.0 0.0 0.0 0.0 8 3.125342811039586E-5 0.0 0.0 0.0 0.0 9 3.125342811039586E-5 0.0 0.0 0.0 0.0 10-11 4.688014216559379E-5 0.0 0.0 0.0 0.0 12-13 6.250685622079172E-5 0.0 0.0 0.0 0.0 14-15 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 16-17 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 18-19 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 20-21 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 22-23 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 24-25 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 26-27 6.250685622079172E-5 0.0 0.0 3.125342811039586E-5 0.0 28-29 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 30-31 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 32-33 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 34-35 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 36-37 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 38-39 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 40-41 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 42-43 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 44-45 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 46-47 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 48-49 6.250685622079172E-5 3.125342811039586E-5 0.0 3.125342811039586E-5 0.0 50-51 9.376028433118758E-5 3.125342811039586E-5 0.0 9.376028433118758E-5 0.0 52-53 1.2501371244158345E-4 3.125342811039586E-5 0.0 9.376028433118758E-5 0.0 54-55 1.562671405519793E-4 6.250685622079172E-5 0.0 9.376028433118758E-5 0.0 56-57 1.8752056866237516E-4 6.250685622079172E-5 1.0938699838638551E-4 9.376028433118758E-5 0.0 58-59 2.1877399677277103E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 60-61 2.656541389383648E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 62-63 3.2816099515915655E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 64-65 3.750411373247503E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 66-67 3.750411373247503E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 68-69 4.219212794903441E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 70-71 5.000548497663338E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 72-73 5.469349919319276E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 74-75 5.938151340975213E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 76-77 7.032021324839069E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 78-79 8.594692730358861E-4 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 80-81 0.001125123411974251 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 82-83 0.0015001645492990013 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 84-85 0.0016876851179613763 6.250685622079172E-5 2.1877399677277103E-4 9.376028433118758E-5 0.0 86-87 0.0017189385460717722 1.2501371244158345E-4 2.1877399677277103E-4 9.376028433118758E-5 0.0 88 0.0017189385460717722 1.2501371244158345E-4 2.1877399677277103E-4 9.376028433118758E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11890 0.0 81.00615 8 CAGCTGC 90 0.0 76.484695 1 CTTCACC 13390 0.0 75.647285 6 CACCGGC 12300 0.0 75.443886 9 GGCGCGC 570 0.0 74.935745 6 CCTTCAC 14790 0.0 72.104546 5 CGGGCCC 1060 0.0 70.40666 7 TTCACCG 14795 0.0 67.73379 7 GGGCCCG 1115 0.0 66.51272 8 CGTAGTG 2315 0.0 66.50363 4 GTGTGGC 4725 0.0 65.067184 7 AACGGGC 1165 0.0 62.4494 5 GCCTTCA 4520 0.0 62.30678 4 GCGCGCA 705 0.0 61.25213 7 ACCTTCA 6550 0.0 60.983273 4 GCGTAGT 1465 0.0 60.874928 3 AGGCGCG 710 0.0 60.820774 5 ACGGGCC 1230 0.0 60.29405 6 CGGGGCA 1290 0.0 59.672832 94 GGCCCGA 1230 0.0 59.530834 9 >>END_MODULE