FastQCFastQC Report
Tue 7 Mar 2017
1HL_27A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_27A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3199649
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG118900.081.006158
CAGCTGC900.076.4846951
CTTCACC133900.075.6472856
CACCGGC123000.075.4438869
GGCGCGC5700.074.9357456
CCTTCAC147900.072.1045465
CGGGCCC10600.070.406667
TTCACCG147950.067.733797
GGGCCCG11150.066.512728
CGTAGTG23150.066.503634
GTGTGGC47250.065.0671847
AACGGGC11650.062.44945
GCCTTCA45200.062.306784
GCGCGCA7050.061.252137
ACCTTCA65500.060.9832734
GCGTAGT14650.060.8749283
AGGCGCG7100.060.8207745
ACGGGCC12300.060.294056
CGGGGCA12900.059.67283294
GGCCCGA12300.059.5308349
Produced by FastQC (version 0.11.5)