FastQCFastQC Report
Tue 7 Mar 2017
1HL_28A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_28A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3770238
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA190920.5063871299371552No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG103800.2753141844095784No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA91830.2435655255715952No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA86660.22985286340013547No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT80610.21380613107183152No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG65980.17500221471429656No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG65280.17314556799862502No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG63790.16919356284669562No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT61030.16187307008204788No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA53830.1427761324351407No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT50500.13394379877344612No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA49270.13068140525876615No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC47800.12678244715585593No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG45840.12158383635197566No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA44480.11797663701867096No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC44170.11715440775887359No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA43880.11638522554809538No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC43450.11524471399418286No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA42380.11240669687165637No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC39900.10582886279327725No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA38170.10124029305311762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCT750.085.999823
GCTGCAC402.1827873E-1185.999823
AGCTGCC3050.085.999822
GCAGCCG550.085.999823
GCTGCTC357.2759576E-1085.999813
GCTGCTA900.085.999813
GCTGCCG258.3600025E-785.999813
CAGCTGC9300.085.537451
CAGCAGC7300.085.4107741
AGCTGCA2450.084.244722
GCTGCCC2000.083.8498153
AGCAGCA3300.083.393762
AGCAGCG1500.083.1331562
AGCAGCC1350.082.814642
AGCTGCT2650.082.754532
GCTGCAT1200.082.416493
TGCTGCA850.080.9412
GCTGCCA800.080.6248253
GCAGCAA1600.080.6248253
TGCAGCA800.080.6248252
Produced by FastQC (version 0.11.5)