##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_28A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3770238 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.099932152824305 12.0 12.0 32.0 2.0 32.0 2 13.294544270149524 12.0 12.0 12.0 12.0 22.0 3 14.283969871398039 12.0 12.0 12.0 12.0 22.0 4 16.55776797114665 12.0 12.0 22.0 12.0 22.0 5 22.772794714816413 22.0 22.0 22.0 22.0 27.0 6 29.80262094859794 32.0 27.0 37.0 22.0 37.0 7 34.55454005821383 37.0 32.0 41.0 22.0 41.0 8 36.03073280784927 37.0 37.0 41.0 27.0 41.0 9 37.346970668695185 41.0 37.0 41.0 27.0 41.0 10-11 38.096929955085066 41.0 37.0 41.0 32.0 41.0 12-13 38.67953230008291 41.0 41.0 41.0 32.0 41.0 14-15 38.446805612802166 41.0 41.0 41.0 32.0 41.0 16-17 38.27236569680747 41.0 37.0 41.0 32.0 41.0 18-19 38.013635346097516 41.0 37.0 41.0 29.5 41.0 20-21 37.195955666459255 41.0 37.0 41.0 27.0 41.0 22-23 37.70542615612065 41.0 37.0 41.0 27.0 41.0 24-25 37.239379450315866 41.0 37.0 41.0 27.0 41.0 26-27 37.02679618634155 41.0 37.0 41.0 27.0 41.0 28-29 37.327111047101 41.0 37.0 41.0 27.0 41.0 30-31 37.49253840208496 41.0 37.0 41.0 27.0 41.0 32-33 37.492565986550446 41.0 37.0 41.0 27.0 41.0 34-35 37.932856891262574 41.0 37.0 41.0 29.5 41.0 36-37 38.061723556974385 41.0 37.0 41.0 32.0 41.0 38-39 37.71321226935806 41.0 37.0 41.0 27.0 41.0 40-41 37.4161834080501 41.0 37.0 41.0 27.0 41.0 42-43 36.97302146442745 41.0 37.0 41.0 27.0 41.0 44-45 37.63119901183957 41.0 37.0 41.0 27.0 41.0 46-47 37.66750149460061 41.0 37.0 41.0 27.0 41.0 48-49 37.660531908065224 41.0 37.0 41.0 27.0 41.0 50-51 37.79779976754783 41.0 37.0 41.0 27.0 41.0 52-53 37.73202712932181 41.0 37.0 41.0 27.0 41.0 54-55 37.45082326367725 41.0 37.0 41.0 27.0 41.0 56-57 36.804569499326035 41.0 37.0 41.0 27.0 41.0 58-59 35.81812567270289 41.0 34.5 41.0 22.0 41.0 60-61 35.92695805941163 41.0 34.5 41.0 22.0 41.0 62-63 35.475460832976594 41.0 32.0 41.0 22.0 41.0 64-65 35.1140931951776 41.0 32.0 41.0 22.0 41.0 66-67 35.57747866315071 41.0 32.0 41.0 22.0 41.0 68-69 36.082368540129295 41.0 37.0 41.0 22.0 41.0 70-71 36.44459620321051 41.0 37.0 41.0 22.0 41.0 72-73 36.87056718965752 41.0 37.0 41.0 27.0 41.0 74-75 36.619103621575086 41.0 37.0 41.0 22.0 41.0 76-77 35.50524595529513 39.0 34.5 41.0 22.0 41.0 78-79 36.258060764333706 41.0 34.5 41.0 22.0 41.0 80-81 37.12343809595044 41.0 37.0 41.0 27.0 41.0 82-83 37.32431613070581 41.0 37.0 41.0 27.0 41.0 84-85 37.32418391093613 41.0 37.0 41.0 27.0 41.0 86-87 37.243568708394534 41.0 37.0 41.0 27.0 41.0 88-89 37.170999417012936 41.0 37.0 41.0 27.0 41.0 90-91 36.952215881331625 41.0 37.0 41.0 27.0 41.0 92-93 36.84738722064761 41.0 37.0 41.0 27.0 41.0 94-95 36.68695477579929 41.0 37.0 41.0 22.0 41.0 96-97 36.55803360424461 41.0 37.0 41.0 22.0 41.0 98-99 36.396024468481826 41.0 37.0 41.0 22.0 41.0 100 36.254504888020335 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2104475611581549 1101 2 -0.5272620052569916 1101 3 -0.4979309520930748 1101 4 -0.8602824416393187 1101 5 -1.3281792133828354 1101 6 -1.8293180043037154 1101 7 -1.0388081130208064 1101 8 -0.9189342412764674 1101 9 -0.4983975626715704 1101 10-11 -0.24940135103772576 1101 12-13 -0.11603339891003372 1101 14-15 -0.1569574282531505 1101 16-17 -0.2043428250478314 1101 18-19 -0.23990842667049606 1101 20-21 -0.34124086431251044 1101 22-23 -0.22969886516593618 1101 24-25 -0.4114186018266963 1101 26-27 -0.28676730895559643 1101 28-29 -0.3335905768549736 1101 30-31 -0.4984888810421211 1101 32-33 -0.43555535691332636 1101 34-35 -0.3282139560047881 1101 36-37 -0.19740583866081352 1101 38-39 -0.16921416725532623 1101 40-41 -0.22729442520125787 1101 42-43 -0.25010859504422456 1101 44-45 -0.3443155655886869 1101 46-47 -0.043223147944814855 1101 48-49 -0.012831235332868118 1101 50-51 -0.09480476793964954 1101 52-53 -0.16664762741084616 1101 54-55 -0.3585642272259264 1101 56-57 -0.39130018786702436 1101 58-59 -0.7351379256425901 1101 60-61 -0.7062712109022797 1101 62-63 -0.7701730086101506 1101 64-65 -0.761515568549342 1101 66-67 -0.36107549898498803 1101 68-69 -0.31194158181076403 1101 70-71 -0.4692476682213069 1101 72-73 -0.334596580367311 1101 74-75 -0.34899900547091534 1101 76-77 -0.7455103966205314 1101 78-79 -0.6698623882248498 1101 80-81 -0.6254488148958544 1101 82-83 -0.5340545680743816 1101 84-85 -0.3995326205236083 1101 86-87 -0.3865342493450754 1101 88-89 -0.6568110036840409 1101 90-91 -0.6565488651872542 1101 92-93 -0.3223382336731646 1101 94-95 -0.33000533547080124 1101 96-97 -0.4655555541091516 1101 98-99 -0.539429874760458 1101 100 -0.654792393594704 1102 1 2.325233832975375 1102 2 0.4105188775099702 1102 3 -0.36338150471213915 1102 4 -0.09867186501048764 1102 5 -0.7266370754708404 1102 6 -0.686244636240243 1102 7 -0.36014720156543945 1102 8 -0.609212688384936 1102 9 -0.6443154715731794 1102 10-11 -0.5289332512503009 1102 12-13 -0.39619709692193084 1102 14-15 -0.26813194380758887 1102 16-17 -0.38800131331201726 1102 18-19 -0.5033914568518014 1102 20-21 -0.5146215764360349 1102 22-23 -0.5761892728196898 1102 24-25 -0.6148906789118271 1102 26-27 -0.5525103672722125 1102 28-29 -0.7329063710292587 1102 30-31 -0.7700402991643216 1102 32-33 -0.8187459357998748 1102 34-35 -0.47470427298721063 1102 36-37 -0.4236627132474098 1102 38-39 -0.4622546442952995 1102 40-41 -0.43373077461045995 1102 42-43 -0.4776058080343404 1102 44-45 -0.05135496998688183 1102 46-47 -0.40475411514944426 1102 48-49 -0.40334470251823973 1102 50-51 -0.4214446382071131 1102 52-53 -0.38386112528846184 1102 54-55 -0.481506294142271 1102 56-57 -0.5934096562655782 1102 58-59 -1.0778836982053548 1102 60-61 -1.0939480709905283 1102 62-63 -1.0393094866366326 1102 64-65 -1.039126506050053 1102 66-67 -0.8659393355880098 1102 68-69 -0.9454786385016476 1102 70-71 -0.6459429160011112 1102 72-73 -0.34646745170515203 1102 74-75 -0.7193193790005097 1102 76-77 -0.49174823929580924 1102 78-79 -0.5457069122887859 1102 80-81 -0.5954231866455686 1102 82-83 -0.6646340167836584 1102 84-85 -0.6248141889924739 1102 86-87 -0.47470603886610263 1102 88-89 -0.4423728644228504 1102 90-91 -0.6206735310067515 1102 92-93 -0.45759011181634435 1102 94-95 -0.32427536102652255 1102 96-97 -0.4011574235705453 1102 98-99 -0.6553389296070833 1102 100 -0.6357846008475931 1103 1 2.5138174521976246 1103 2 0.5136795314793297 1103 3 1.0653657297723704 1103 4 2.454953936419308 1103 5 2.19845662442059 1103 6 -0.35220421598211615 1103 7 -1.202622172220103 1103 8 -1.2019555823882797 1103 9 -1.089474819540321 1103 10-11 -0.9059327007474849 1103 12-13 -0.6861294953271155 1103 14-15 -0.6326019699597794 1103 16-17 -0.790011669799938 1103 18-19 -1.0816717851413529 1103 20-21 -1.0133427589561848 1103 22-23 -0.9698891358842374 1103 24-25 -1.0758612866756394 1103 26-27 -1.2297705728020958 1103 28-29 -0.9874770951342953 1103 30-31 -1.0732377636364703 1103 32-33 -1.3580676467346393 1103 34-35 -1.031619617151705 1103 36-37 -0.6794896560391805 1103 38-39 -0.7009597391222968 1103 40-41 -1.0962565670196511 1103 42-43 -0.95268118304665 1103 44-45 -0.966496454506327 1103 46-47 -0.8866504961843873 1103 48-49 -0.8014730307578617 1103 50-51 -0.7547667194216885 1103 52-53 -0.5038285678974503 1103 54-55 -0.94881047370869 1103 56-57 -1.2815833295982202 1103 58-59 -1.7428219354200678 1103 60-61 -1.34616322549536 1103 62-63 -1.4608413236083777 1103 64-65 -1.5464942799441133 1103 66-67 -1.4769303295434497 1103 68-69 -1.2551166171823809 1103 70-71 -1.45785519372685 1103 72-73 -1.2685754633460888 1103 74-75 -1.3318116798091708 1103 76-77 -1.293746691231199 1103 78-79 -1.2209293969343733 1103 80-81 -0.8017936162287711 1103 82-83 -0.8426703821185768 1103 84-85 -0.8592769041041421 1103 86-87 -0.732666014606913 1103 88-89 -0.6517394306525404 1103 90-91 -1.1065718368158883 1103 92-93 -0.8833035765176902 1103 94-95 -0.8992113515997033 1103 96-97 -0.6486035235578242 1103 98-99 -0.7932117080464423 1103 100 -0.7668975980281303 1104 1 2.800110433649401 1104 2 -0.6605050514076325 1104 3 -0.25182741775752326 1104 4 0.046829712253522615 1104 5 -1.0650426547739222 1104 6 -0.49541003381765947 1104 7 -0.5143984145467826 1104 8 -0.9171514468730635 1104 9 -0.6319513295947559 1104 10-11 -0.554216843593565 1104 12-13 -0.2879856403119945 1104 14-15 -0.39305856197631783 1104 16-17 -0.3348668379658477 1104 18-19 -0.5323874892540061 1104 20-21 -0.5261054561857605 1104 22-23 -0.5226757151095498 1104 24-25 -0.6547294992480772 1104 26-27 -0.7699924637497944 1104 28-29 -0.5452158820271649 1104 30-31 -0.6937954573612117 1104 32-33 -0.7847545488581389 1104 34-35 -0.7968596935589787 1104 36-37 -0.7388795881908834 1104 38-39 -0.6821093057887779 1104 40-41 -0.6815847271801729 1104 42-43 -0.588777478125337 1104 44-45 -0.47921503428334233 1104 46-47 -0.5809720982430804 1104 48-49 -0.8292555005416489 1104 50-51 -0.6551570691304391 1104 52-53 -0.66302360860454 1104 54-55 -0.8412922740786328 1104 56-57 -1.0351435229970463 1104 58-59 -1.3581688573066657 1104 60-61 -1.1481700500023706 1104 62-63 -1.125949425537641 1104 64-65 -1.1192570905375874 1104 66-67 -1.6188292505080284 1104 68-69 -1.2260943241706954 1104 70-71 -0.812976408080992 1104 72-73 -0.9167224358685573 1104 74-75 -0.9154452385477327 1104 76-77 -0.8298081269094624 1104 78-79 -0.8581224877823175 1104 80-81 -0.8507145808044427 1104 82-83 -0.8892353613635393 1104 84-85 -0.7216587930868812 1104 86-87 -0.6604294014668994 1104 88-89 -0.6362275642859174 1104 90-91 -0.5371326669419716 1104 92-93 -0.8540165787906417 1104 94-95 -0.787442477926767 1104 96-97 -0.7598896559404125 1104 98-99 -0.9146109398170523 1104 100 -1.0819668728203453 1105 1 1.746901804940471 1105 2 0.3076714680242727 1105 3 -0.3408273194868183 1105 4 -0.21695996679795826 1105 5 -0.9300975917048646 1105 6 0.2773633908984152 1105 7 -0.08004640166520716 1105 8 -0.3172800102004203 1105 9 -0.16697401750592178 1105 10-11 -0.14733595580338488 1105 12-13 -0.06779596883367134 1105 14-15 -0.19395634150914276 1105 16-17 -0.18313659736489285 1105 18-19 -0.25784108970334785 1105 20-21 -0.40211906442463174 1105 22-23 -0.5263320615489491 1105 24-25 -0.5505442941282581 1105 26-27 -0.38495764977444225 1105 28-29 -0.35289614340921105 1105 30-31 -0.3405367917331432 1105 32-33 -0.5681236743660634 1105 34-35 -0.31954814566913825 1105 36-37 -0.23145000726586318 1105 38-39 -0.12266774485326692 1105 40-41 -0.3777723196470433 1105 42-43 -0.13910407044886597 1105 44-45 0.014243382332161048 1105 46-47 -0.1816751394960363 1105 48-49 -0.1490075518299463 1105 50-51 -0.1698658497532648 1105 52-53 -0.19483536881319452 1105 54-55 -0.32734635362020015 1105 56-57 -0.5706920944204654 1105 58-59 -0.7169198636798342 1105 60-61 -0.719372225301079 1105 62-63 -0.7915466995623532 1105 64-65 -1.1718946672721415 1105 66-67 -0.7488983735817101 1105 68-69 -0.7038958732415921 1105 70-71 -0.7439492610238361 1105 72-73 -0.43693158117486774 1105 74-75 -0.5207985577985994 1105 76-77 -0.5965470090215845 1105 78-79 -0.5566692533688169 1105 80-81 -0.6359224053163999 1105 82-83 -0.5164135676256407 1105 84-85 -0.34165972169996905 1105 86-87 -0.3485994566697954 1105 88-89 -0.3520016805031929 1105 90-91 -0.3435531249195165 1105 92-93 -0.3003783989083644 1105 94-95 -0.21188691035033003 1105 96-97 -0.3586091615936766 1105 98-99 -0.3483037726369389 1105 100 -0.6108155458502651 1106 1 0.33618125930759746 1106 2 -0.3945590348588617 1106 3 -0.33948633667499273 1106 4 -0.7182834085825576 1106 5 -1.2960352320717412 1106 6 -1.2035304538976916 1106 7 -0.8846764728888488 1106 8 -0.5869454237219642 1106 9 -0.6017953159714082 1106 10-11 -0.47204926604971575 1106 12-13 -0.3226510998315604 1106 14-15 -0.18768553473588412 1106 16-17 -0.27643701188985403 1106 18-19 -0.2590800075960473 1106 20-21 -0.4867679527079076 1106 22-23 -0.4714028429076009 1106 24-25 -0.5811481729309804 1106 26-27 -0.7197793193850899 1106 28-29 -0.7510303171565766 1106 30-31 -0.5598507970636746 1106 32-33 -0.5647922930795346 1106 34-35 -0.4988786828655236 1106 36-37 -0.6961185344301981 1106 38-39 -0.4547850033948464 1106 40-41 -0.4041444366776048 1106 42-43 -0.17437571821938036 1106 44-45 -0.30840530040738656 1106 46-47 -0.4499822942822149 1106 48-49 -0.4139588474225917 1106 50-51 -0.5995287606960034 1106 52-53 -0.5541254017010786 1106 54-55 -0.7598511318932282 1106 56-57 -0.9407334072952125 1106 58-59 -1.1622358217641136 1106 60-61 -1.0299102205357542 1106 62-63 -1.1463052267770522 1106 64-65 -1.2940624872723916 1106 66-67 -1.1933594606464482 1106 68-69 -1.1630933230855192 1106 70-71 -0.970359075798541 1106 72-73 -0.833001834358619 1106 74-75 -0.8519678497428913 1106 76-77 -0.7903837106297971 1106 78-79 -0.7924929000343823 1106 80-81 -0.8242768068815067 1106 82-83 -0.6936668407062072 1106 84-85 -1.0099089248203796 1106 86-87 -0.8049896352144756 1106 88-89 -0.8258131919591705 1106 90-91 -0.954508472887106 1106 92-93 -0.7914182036082451 1106 94-95 -0.9011997501083187 1106 96-97 -0.7659544070960465 1106 98-99 -1.087855439887278 1106 100 -1.1997400263818037 1107 1 1.6735482327077982 1107 2 -0.4432030950334127 1107 3 -0.42996251133618735 1107 4 -0.6016406197797117 1107 5 -1.1554559357370238 1107 6 -0.5056141696493555 1107 7 -0.8280089657620664 1107 8 -0.7063918007658998 1107 9 -0.6926536022783196 1107 10-11 -0.37221813300745765 1107 12-13 -0.39507952301379134 1107 14-15 -0.5913049407654611 1107 16-17 -0.5957916444392879 1107 18-19 -0.5790391341267025 1107 20-21 -0.9219073058719687 1107 22-23 -0.8855293767061667 1107 24-25 -0.8554249860247154 1107 26-27 -0.9882054349971483 1107 28-29 -0.7414992930031161 1107 30-31 -1.0033901436572492 1107 32-33 -1.0017399669423526 1107 34-35 -0.7617432204722832 1107 36-37 -0.7390670935138388 1107 38-39 -0.7635831846259933 1107 40-41 -0.7767970912674329 1107 42-43 -0.8947194966258678 1107 44-45 -0.3953749252692589 1107 46-47 -0.4791451233014783 1107 48-49 -0.5324628260243358 1107 50-51 -0.5956181078595648 1107 52-53 -0.41440345546832447 1107 54-55 -0.8811089263828649 1107 56-57 -0.8851028167132569 1107 58-59 -1.2371793420365407 1107 60-61 -1.1930389061695337 1107 62-63 -1.1718015971652207 1107 64-65 -1.501654759335878 1107 66-67 -1.3244090792394232 1107 68-69 -1.2921287995083688 1107 70-71 -0.7276801221974196 1107 72-73 -0.6900208536807853 1107 74-75 -0.7518633053609847 1107 76-77 -0.9218961806912134 1107 78-79 -1.3333431213350977 1107 80-81 -1.020085597747233 1107 82-83 -0.8088753419856971 1107 84-85 -0.5701061849084397 1107 86-87 -0.6117218374243336 1107 88-89 -0.723592178116526 1107 90-91 -0.5095451163796341 1107 92-93 -0.652611548061742 1107 94-95 -0.46707972037596335 1107 96-97 -0.5316283225142797 1107 98-99 -0.7275302168294928 1107 100 -1.0168185948014639 1108 1 2.977134993697593 1108 2 -0.7074960891282984 1108 3 -0.4372900396725736 1108 4 -0.5177915866102119 1108 5 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0.8587450375323087 2224 34-35 0.6406007437881698 2224 36-37 0.4660697895964887 2224 38-39 0.6479533650499505 2224 40-41 0.7577356044267916 2224 42-43 0.9580741439434348 2224 44-45 0.6808383752980163 2224 46-47 0.5281902524978221 2224 48-49 0.4020093354531653 2224 50-51 0.4639053859119997 2224 52-53 0.6178775207293725 2224 54-55 0.7281410808343338 2224 56-57 0.9945990500140311 2224 58-59 1.2610621885860098 2224 60-61 1.2164029430921488 2224 62-63 1.457987687827341 2224 64-65 1.429444085552177 2224 66-67 1.1081700121879976 2224 68-69 1.1310184114069202 2224 70-71 0.9614791558666411 2224 72-73 0.8833607356175506 2224 74-75 0.789672730089876 2224 76-77 0.912384093634337 2224 78-79 0.7205069360415948 2224 80-81 0.6162564485438509 2224 82-83 0.4789360348384477 2224 84-85 0.4631869125669894 2224 86-87 0.7060529392035022 2224 88-89 0.6010368580993486 2224 90-91 0.7430738680889633 2224 92-93 0.5386306122610094 2224 94-95 0.8542556590319421 2224 96-97 0.6730592902543862 2224 98-99 0.7496621863518413 2224 100 0.9508467511186538 2225 1 2.132537363714505 2225 2 1.2842517294011024 2225 3 1.3035835448957176 2225 4 3.015508476460951 2225 5 3.002265733635184 2225 6 1.2225992574044149 2225 7 0.8590497377148409 2225 8 0.8303072396779712 2225 9 0.6077214117395329 2225 10-11 0.44688991011135926 2225 12-13 0.41358187819717074 2225 14-15 0.5448607304641016 2225 16-17 0.5242953982134253 2225 18-19 0.6095210958563797 2225 20-21 0.8013886683787703 2225 22-23 0.8132940451585569 2225 24-25 0.9561061103934705 2225 26-27 0.9789468678543045 2225 28-29 0.7389312130975014 2225 30-31 0.6885705553413857 2225 32-33 0.8112288780878174 2225 34-35 0.6387809363795256 2225 36-37 0.5438705570396394 2225 38-39 0.5470793808858616 2225 40-41 0.6064399787831931 2225 42-43 1.011679578665536 2225 44-45 0.6899334116176234 2225 46-47 0.5829845109376066 2225 48-49 0.4558560312033535 2225 50-51 0.6166289219950443 2225 52-53 0.6383774024471052 2225 54-55 0.7387320398947494 2225 56-57 1.1200623512024208 2225 58-59 1.434677590328235 2225 60-61 1.4052145716301752 2225 62-63 1.4548466849329103 2225 64-65 1.3044146019586265 2225 66-67 1.3116813429330278 2225 68-69 1.2460895721038057 2225 70-71 0.9363872261958832 2225 72-73 0.8840449463269735 2225 74-75 0.9041163859859012 2225 76-77 0.9935920860364504 2225 78-79 0.8058916838916375 2225 80-81 0.6643499297995916 2225 82-83 0.606260020394096 2225 84-85 0.42916023643773826 2225 86-87 0.6091535000005877 2225 88-89 0.5370941623770022 2225 90-91 0.6626237518940314 2225 92-93 0.7228857644972422 2225 94-95 0.9350697541030542 2225 96-97 0.8852321876132123 2225 98-99 0.8325443076398642 2225 100 0.9704610813627355 2226 1 1.6557249224774964 2226 2 1.1279104085361116 2226 3 1.2921739954331617 2226 4 2.280647093593064 2226 5 3.0316408765721903 2226 6 0.5727809462210232 2226 7 0.8052698479986802 2226 8 1.078367108048866 2226 9 0.8770147311815037 2226 10-11 0.6201638663535789 2226 12-13 0.506755206787112 2226 14-15 0.520253966049701 2226 16-17 0.5563768151320829 2226 18-19 0.5609911235543947 2226 20-21 0.9686541881612527 2226 22-23 0.8155784961533996 2226 24-25 0.9711900938254843 2226 26-27 1.0337724211793429 2226 28-29 0.8238307125003459 2226 30-31 0.9449893798274474 2226 32-33 1.046153788513962 2226 34-35 0.7608415162590774 2226 36-37 0.6828040563916602 2226 38-39 0.8085969271974491 2226 40-41 0.9502168191765534 2226 42-43 1.1789768622271453 2226 44-45 0.6646799366601854 2226 46-47 0.5783660574524845 2226 48-49 0.6191027976506405 2226 50-51 0.5611172710408638 2226 52-53 0.49901953900594265 2226 54-55 0.8955587113048082 2226 56-57 1.1466308466157358 2226 58-59 1.3093898104893498 2226 60-61 1.5539986541651842 2226 62-63 1.635524142814127 2226 64-65 1.692457444943912 2226 66-67 1.3564657304003163 2226 68-69 1.3342353297334668 2226 70-71 1.1913220469211154 2226 72-73 0.9135293566641778 2226 74-75 0.980086451800446 2226 76-77 1.1333501730946125 2226 78-79 0.9200926342639093 2226 80-81 0.7082843185374301 2226 82-83 0.6791388352239736 2226 84-85 0.6467942160580975 2226 86-87 0.8001224223190917 2226 88-89 0.7229491162559896 2226 90-91 0.8368544331635732 2226 92-93 0.7478266838680625 2226 94-95 0.8518537349939592 2226 96-97 0.8270981190542699 2226 98-99 0.857871474564881 2226 100 0.9494055074547774 2227 1 1.390397460760326 2227 2 1.2171041629134027 2227 3 0.9796098473996508 2227 4 -0.06969744739808803 2227 5 3.004941860488831 2227 6 1.0092117451584706 2227 7 0.9230634601531804 2227 8 0.9689066948862717 2227 9 0.8089837824981387 2227 10-11 0.620236768970365 2227 12-13 0.36867514287641967 2227 14-15 0.4409688774112226 2227 16-17 0.5316900097892301 2227 18-19 0.5380709528153957 2227 20-21 0.8106494987496831 2227 22-23 0.703363188036846 2227 24-25 0.9430517436457322 2227 26-27 0.8434976660837705 2227 28-29 0.6176488124109625 2227 30-31 0.7574076778468992 2227 32-33 0.746912280235847 2227 34-35 0.5601710629552628 2227 36-37 0.5982825178777631 2227 38-39 0.6030603585641998 2227 40-41 0.760687793366877 2227 42-43 0.9542333648002312 2227 44-45 0.6181602305634186 2227 46-47 0.5781667843291984 2227 48-49 0.44284661570577555 2227 50-51 0.4385101606175823 2227 52-53 0.5593745765409395 2227 54-55 0.6902325213531881 2227 56-57 1.0960364753425864 2227 58-59 1.5755911184375222 2227 60-61 1.3920669855908656 2227 62-63 1.4454668864960567 2227 64-65 1.6373448578682712 2227 66-67 1.3148461727956047 2227 68-69 0.9919113752232676 2227 70-71 0.9122893410518245 2227 72-73 0.8265907023354302 2227 74-75 0.7746829707452747 2227 76-77 0.9223191294766409 2227 78-79 0.8007270034592366 2227 80-81 0.680055198330507 2227 82-83 0.47279644190449943 2227 84-85 0.5364481346185315 2227 86-87 0.6693583240814718 2227 88-89 0.6738137824891837 2227 90-91 0.7367369291915438 2227 92-93 0.5807874436315501 2227 94-95 0.7485134248622884 2227 96-97 0.6899366370712414 2227 98-99 0.8618549666897906 2227 100 0.69024127236802 2228 1 1.1733438582511901 2228 2 1.0508669390556253 2228 3 -0.12098472535285865 2228 4 -0.402341573127746 2228 5 3.0173134868263602 2228 6 0.7590997926543217 2228 7 0.8402702101894732 2228 8 1.0385230476486171 2228 9 1.0119981911352767 2228 10-11 0.6236896290944642 2228 12-13 0.5081886042904102 2228 14-15 0.531646732331275 2228 16-17 0.6876953432765021 2228 18-19 0.5606884198549977 2228 20-21 0.8240551114185095 2228 22-23 0.8701286003106858 2228 24-25 0.858698243118333 2228 26-27 0.9622285445085978 2228 28-29 0.722788700563413 2228 30-31 0.6899407694327948 2228 32-33 0.6459432788346859 2228 34-35 0.5935124428748324 2228 36-37 0.5791944409943781 2228 38-39 0.6071797734024571 2228 40-41 0.6578425816979276 2228 42-43 0.8590805146469123 2228 44-45 0.5886727718677705 2228 46-47 0.402527036991998 2228 48-49 0.49928174185350827 2228 50-51 0.5066158980084907 2228 52-53 0.45244882729006264 2228 54-55 0.692110874461882 2228 56-57 1.1257682179008128 2228 58-59 1.4791354647485946 2228 60-61 1.3581482631088804 2228 62-63 1.4396026306566014 2228 64-65 1.5481764375705893 2228 66-67 1.1813923593259688 2228 68-69 1.3010323119158755 2228 70-71 1.2541417351736257 2228 72-73 0.8968461901947009 2228 74-75 0.813518857120485 2228 76-77 0.9853480394615772 2228 78-79 0.7835932412078748 2228 80-81 0.803984565046683 2228 82-83 0.8151621120189532 2228 84-85 0.6444382198248846 2228 86-87 0.6490726177923634 2228 88-89 0.7259357634887422 2228 90-91 0.8375195471658614 2228 92-93 0.724349037625295 2228 94-95 0.9023736434145633 2228 96-97 0.6716001725663148 2228 98-99 0.8846749103286982 2228 100 0.9800047027326855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 36.0 13 35.0 14 214.0 15 741.0 16 2471.0 17 6327.0 18 11036.0 19 14651.0 20 18219.0 21 22209.0 22 26016.0 23 30303.0 24 35351.0 25 40042.0 26 45586.0 27 51113.0 28 58097.0 29 66257.0 30 75783.0 31 87320.0 32 103541.0 33 126410.0 34 158101.0 35 208029.0 36 294339.0 37 469384.0 38 895026.0 39 921332.0 40 2269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 3.5788113115247047 8.07281311569584 82.98151996182219 5.366855610957273 2 51.472846791148385 14.090533154304879 6.629826487051119 27.806793567495617 3 8.451827179079942 12.910139890372967 46.72174541766329 31.9162875128838 4 84.8735013545564 7.292033022848956 4.9528703492989035 2.881595273295744 5 17.348931287626932 36.016161313954186 26.091960242297702 20.542947156121176 6 17.736386933663074 28.104591805610152 30.885901632735123 23.273119627991655 7 20.366671812230422 26.94686648429091 35.9813624497976 16.705099253681066 8 20.821470686996417 28.00953149376777 33.874015380461394 17.294982438774422 9 20.916239240069196 28.221746213369027 27.877364771136463 22.984649775425318 10-11 19.684791782375544 30.15244660947134 28.520905046312727 21.64185656184039 12-13 24.258760322292652 26.591570611722652 27.361575051760656 21.788094014224036 14-15 21.85916379814749 27.354029109037683 28.521501825614195 22.265305267200638 16-17 20.973079153093252 29.504065791072076 28.302218586731133 21.220636469103543 18-19 21.5874037660222 29.481520795238918 29.648393549691026 19.282681889047854 20-21 23.219489061433258 28.839054722805297 29.6340841082181 18.307372107543344 22-23 21.99048176799449 30.507928146711162 28.49017754316836 19.01141254212599 24-25 22.27678995331329 28.707073664845563 26.4989372023729 22.517199179468246 26-27 20.402544879129646 26.730580403677433 29.806884870398104 23.059989846794817 28-29 19.890295686557742 27.98751692703792 30.61033086747729 21.51185651892705 30-31 20.25999119065079 30.052056330496224 28.168338413762743 21.51961406509024 32-33 20.876281550395493 30.206183270127774 28.585198069723983 20.33233710975275 34-35 21.485818667150454 29.2576489866157 29.776568481883636 19.479963864350207 36-37 21.882968661394848 28.169256158364536 28.856852007751232 21.090923172489376 38-39 21.594828283599885 29.33997648399992 28.850837847020806 20.21435738537939 40-41 21.081858160618324 29.73277137961583 29.77150958309683 19.413860876669016 42-43 20.621839469624362 29.8021992537067 29.285997803584312 20.289963473084622 44-45 20.658748864130065 30.013834670384202 28.80704082872222 20.520375636763514 46-47 20.090142302030422 27.881357505669534 28.92514886675596 23.10335132554408 48-49 21.0354520106492 28.12395770823456 30.205179381935487 20.635410899180755 50-51 21.90953197119121 30.60656913436234 28.266106808111317 19.217792086335134 52-53 20.356468155056152 30.281292749091058 28.33597577836086 21.026263317491924 54-55 22.4082442644002 28.27401366041249 28.38982460696768 20.927917468219636 56-57 21.413608496171673 29.039652585606845 29.175508887492995 20.371230030728483 58-59 21.170423879859015 28.39169806335716 28.921305829982774 21.516572226801046 60-61 19.50834823185167 30.8419819005713 29.297752298633277 20.351917568943747 62-63 20.24181702308549 31.7724251401105 27.128601905807812 20.857155930996203 64-65 19.7466151632253 30.930577641941802 28.450558831435774 20.872248363397123 66-67 20.066810105887214 30.708591871388492 29.24936038520645 19.975237637517843 68-69 20.790305545697645 30.38870490404054 29.041084408994873 19.779905141266944 70-71 19.626930713657863 31.09759914360844 29.107830858423263 20.167639284310436 72-73 19.991708746238302 31.02853188578546 28.387027556350553 20.592731811625686 74-75 20.033761794348262 29.492342393238836 30.254588702357783 20.21930711005512 76-77 19.552266992163357 30.718617763653118 28.646427626054376 21.08268761812915 78-79 19.59985019513357 29.655886445364988 28.480788215491966 22.263475144009476 80-81 21.01502043841098 29.486467600977605 28.220231686161277 21.278280274450136 82-83 20.453324166803263 28.163275103587626 29.792853925932526 21.590546803676585 84-85 20.834268287572296 29.270658775387652 28.087470870539207 21.80760206650084 86-87 21.128825289013584 28.990225550747724 28.441440566881987 21.439508593356706 88-89 20.458310589411067 29.204681508170044 28.13862148755596 22.198386414862934 90-91 20.343145445990412 29.76292212852345 27.131006053198764 22.762926372287374 92-93 20.522776015731633 29.40461583592335 28.130226792048674 21.94238135629634 94-95 20.610369955424567 29.617056005482944 27.584797034033393 22.187777005059097 96-97 20.419798431823136 29.4730200056336 27.753115320571276 22.354066241971992 98-99 21.745417663288098 30.301880146558386 26.86463029654892 21.088071893604596 100 20.890564468343907 31.501990060043955 26.068142117288083 21.53930335432405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 10.0 2 19.0 3 22.0 4 32.5 5 42.0 6 56.0 7 64.0 8 78.0 9 90.0 10 111.5 11 137.5 12 137.5 13 157.5 14 211.0 15 256.5 16 330.0 17 460.0 18 644.5 19 1011.5 20 1635.5 21 2545.5 22 3772.5 23 5698.5 24 8773.0 25 13076.5 26 19171.0 27 26670.5 28 34500.5 29 43716.0 30 54619.0 31 66327.0 32 79464.5 33 94356.5 34 109167.0 35 124682.5 36 143879.5 37 160299.0 38 169807.5 39 176771.0 40 180710.0 41 182454.5 42 181917.5 43 178694.5 44 178911.5 45 175059.0 46 160189.5 47 146281.5 48 137983.5 49 133886.0 50 131199.0 51 120375.0 52 102153.5 53 85540.5 54 72565.5 55 60147.0 56 46540.0 57 33807.0 58 23814.5 59 16990.5 60 13153.0 61 11125.0 62 9471.0 63 7224.5 64 5333.5 65 5199.0 66 5265.5 67 4027.5 68 2882.5 69 2633.0 70 3427.5 71 3602.0 72 2124.0 73 860.0 74 384.5 75 236.0 76 167.0 77 125.5 78 104.0 79 80.0 80 59.0 81 46.0 82 32.0 83 19.5 84 15.5 85 17.0 86 19.5 87 18.5 88 20.0 89 21.0 90 19.0 91 16.5 92 24.5 93 25.0 94 15.0 95 14.5 96 17.0 97 23.0 98 49.0 99 112.5 100 141.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.351617590189267 2 0.006896116372494256 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.315440563699162E-4 30-31 0.010065677551390654 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010344174558741385 40-41 0.0015383644215564113 42-43 1.1935586029316982E-4 44-45 0.0 46-47 0.0023340701568442097 48-49 1.3261762254796648E-5 50-51 0.0 52-53 0.0014720556102824278 54-55 0.019428481703277087 56-57 0.011829491931278609 58-59 0.012386485945980068 60-61 2.25449958331543E-4 62-63 0.011816230169023813 64-65 1.1935586029316982E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.25449958331543E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3770238.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.5178791165307 #Duplication Level Percentage of deduplicated Percentage of total 1 76.67935712638115 43.337546368020966 2 11.476407367243079 12.972444085478132 3 4.572939282065856 7.753584886530981 4 2.3303326906652093 5.268218452492642 5 1.3888330502293327 3.924694922295201 6 0.8981740322480246 3.045773482812048 7 0.6143797172778802 2.4306407014931675 8 0.4235330421788393 1.9149751423776111 9 0.3120598520050973 1.5873264893471892 >10 1.218947857481254 11.018588277807638 >50 0.05204694152101611 2.021740673796797 >100 0.029909776363606373 3.18780519571691 >500 0.0021651077492190773 0.8290520378974828 >1k 9.141565902921363E-4 0.7076092839332001 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.6523524509593293E-5 0.0 3 0.0 0.0 0.0 2.6523524509593293E-5 0.0 4 0.0 0.0 0.0 2.6523524509593293E-5 0.0 5 0.0 0.0 0.0 2.6523524509593293E-5 0.0 6 0.0 0.0 0.0 2.6523524509593293E-5 0.0 7 0.0 0.0 0.0 2.6523524509593293E-5 0.0 8 0.0 0.0 0.0 2.6523524509593293E-5 0.0 9 0.0 0.0 0.0 2.6523524509593293E-5 0.0 10-11 0.0 0.0 0.0 2.6523524509593293E-5 0.0 12-13 0.0 0.0 0.0 2.6523524509593293E-5 0.0 14-15 0.0 0.0 0.0 2.6523524509593293E-5 0.0 16-17 0.0 0.0 0.0 2.6523524509593293E-5 0.0 18-19 1.3261762254796647E-5 0.0 0.0 2.6523524509593293E-5 0.0 20-21 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 22-23 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 24-25 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 26-27 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 28-29 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 30-31 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 32-33 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 34-35 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 36-37 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 38-39 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 40-41 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 42-43 2.6523524509593293E-5 0.0 0.0 2.6523524509593293E-5 0.0 44-45 3.978528676438994E-5 0.0 0.0 2.6523524509593293E-5 0.0 46-47 5.304704901918659E-5 0.0 0.0 2.6523524509593293E-5 0.0 48-49 5.304704901918659E-5 0.0 0.0 5.304704901918659E-5 0.0 50-51 9.283233578357653E-5 0.0 0.0 7.957057352877989E-5 0.0 52-53 1.3261762254796646E-4 0.0 0.0 7.957057352877989E-5 0.0 54-55 1.5914114705755977E-4 0.0 0.0 7.957057352877989E-5 0.0 56-57 1.989264338219497E-4 0.0 1.989264338219497E-4 1.0609409803837317E-4 0.0 58-59 2.25449958331543E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 60-61 2.7849700735072956E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 62-63 3.580675808795095E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 64-65 3.978528676438994E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 66-67 5.437322524466624E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 68-69 6.100410637206457E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 70-71 6.76349874994629E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 72-73 7.559204485234089E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 74-75 8.620145465617821E-4 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 76-77 0.0011007262671481216 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 78-79 0.0011802968406769015 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 80-81 0.0014322703235180377 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 82-83 0.001763814379887954 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 84-85 0.0018168614289071406 0.0 3.978528676438994E-4 1.0609409803837317E-4 0.0 86-87 0.0018566467156715305 7.957057352877989E-5 3.978528676438994E-4 1.0609409803837317E-4 0.0 88 0.0018566467156715305 7.957057352877989E-5 3.978528676438994E-4 1.0609409803837317E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTCA 13440 0.0 81.444756 4 TGCGCCT 3230 0.0 79.831215 1 TCACCGG 14395 0.0 78.552315 8 CACCGGC 14135 0.0 76.9089 9 TGTGCCT 5045 0.0 76.404076 1 GTGCAGC 240 0.0 76.27891 2 GCGCCTT 3120 0.0 74.92484 2 CTTCACC 15530 0.0 74.56441 6 GTGCCTT 4750 0.0 73.820694 2 GTGCTGC 225 0.0 73.01913 2 TGCCTTC 8140 0.0 72.31143 3 TGCGACT 1830 0.0 70.30747 1 CCTTCAC 17450 0.0 69.72257 5 TGCTGCG 160 0.0 69.49373 1 TGCGAAC 1620 0.0 67.65527 1 CGGGGCA 1510 0.0 66.83368 94 CGCCTTC 5300 0.0 66.777374 3 TTCACCG 17645 0.0 65.946045 7 TGCGATG 2850 0.0 65.02337 1 CTGCTGC 190 0.0 64.23488 2 >>END_MODULE